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Articles 4981 - 5010 of 20444

Full-Text Articles in Entire DC Network

Consensus Guidelines For Advancing Coral Holobiont Genome And Specimen Voucher Deposition, Christian R. Voolstra, Kate M. Quigley, Sarah W. Davies, John Everett Parkinson, Raquel S. Peixoto, Manuel Aranda, Andrew C. Baker, Adam R. Barno, Daniel J. Barshis, Francesca Benzoni, Victor Bonito, David G. Bourne, Carol Buitrago-López, Tom C.L. Bridge, Cheong Xin Chan, David J. Combosch, Jamie Craggs, Jörg C. Frommlet, Santiago Herrera, Andrea M. Quattrini, Till Röthig, James D. Reimer, Esther Rubio-Portillo, David J. Suggett, Helena Villela, Maren Ziegler, Michael Sweet Jan 2021

Consensus Guidelines For Advancing Coral Holobiont Genome And Specimen Voucher Deposition, Christian R. Voolstra, Kate M. Quigley, Sarah W. Davies, John Everett Parkinson, Raquel S. Peixoto, Manuel Aranda, Andrew C. Baker, Adam R. Barno, Daniel J. Barshis, Francesca Benzoni, Victor Bonito, David G. Bourne, Carol Buitrago-López, Tom C.L. Bridge, Cheong Xin Chan, David J. Combosch, Jamie Craggs, Jörg C. Frommlet, Santiago Herrera, Andrea M. Quattrini, Till Röthig, James D. Reimer, Esther Rubio-Portillo, David J. Suggett, Helena Villela, Maren Ziegler, Michael Sweet

Biological Sciences Faculty Publications

Coral research is being ushered into the genomic era. To fully capitalize on the potential discoveries from this genomic revolution, the rapidly increasing number of high-quality genomes requires effective pairing with rigorous taxonomic characterizations of specimens and the contextualization of their ecological relevance. However, to date there is no formal framework that genomicists, taxonomists, and coral scientists can collectively use to systematically acquire and link these data. Spurred by the recently announced “Coral symbiosis sensitivity to environmental change hub” under the “Aquatic Symbiosis Genomics Project” - a collaboration between the Wellcome Sanger Institute and the Gordon and Betty Moore Foundation …

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Spatio-Temporal Modelling Of Tick Life-Stage Count Data With Spatially Varying Coefficients, Thabo Lephoto, Henry Mwambi, Oliver Bodhlyera, Holly Gaff Jan 2021

Spatio-Temporal Modelling Of Tick Life-Stage Count Data With Spatially Varying Coefficients, Thabo Lephoto, Henry Mwambi, Oliver Bodhlyera, Holly Gaff

Biological Sciences Faculty Publications

There is a vast amount of geo-referenced data in many fields of study including ecological studies. Geo-referencing is usually by point referencing; that is, latitudes and longitudes or by areal referencing, which includes districts, counties, states, provinces and other administrative units. The availability of large geo-referenced datasets for modelling has necessitated the development and application of spatial statistical methods. However, spatial varying coefficients models exploring the abundance of tick counts remain limited. In this study we used data that was collected and prepared by researchers in the Department of Biological Sciences from the Old Dominion University, Virginia, USA. We modelled …

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Variation In Coral Thermotolerance Across A Pollution Gradient Erodes As Coral Symbionts Shift To More Heat-Tolerant Genera, Melissa S. Naugle, Thomas A. Oliver, Daniel J. Barshis, Ruth D. Gates, Cheryl A. Logan Jan 2021

Variation In Coral Thermotolerance Across A Pollution Gradient Erodes As Coral Symbionts Shift To More Heat-Tolerant Genera, Melissa S. Naugle, Thomas A. Oliver, Daniel J. Barshis, Ruth D. Gates, Cheryl A. Logan

Biological Sciences Faculty Publications

Phenotypic plasticity is one mechanism whereby species may cope with stressful environmental changes associated with climate change. Reef building corals present a good model for studying phenotypic plasticity because they have experienced rapid climate-driven declines in recent decades (within a single generation of many corals), often with differential survival among individuals during heat stress. Underlying differences in thermotolerance may be driven by differences in baseline levels of environmental stress, including pollution stress. To examine this possibility, acute heat stress experiments were conducted on Acropora hyacinthus from 10 sites around Tutuila, American Samoa with differing nutrient pollution impact. A threshold-based heat …

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Self And Microbiota-Derived Epitopes Induce Cd4⁺ T Cell Anergy And Conversion Into Cd4⁺Foxp3⁺ Regulatory Cells, Michal P. Kuczma, Edyta A. Szurek, Anna Cebula, Vu L. Ngo, Maciej Pietrzak, Piotr Kraj, Timothy L. Denning, Leszek Ignatowicz Jan 2021

Self And Microbiota-Derived Epitopes Induce Cd4⁺ T Cell Anergy And Conversion Into Cd4⁺Foxp3⁺ Regulatory Cells, Michal P. Kuczma, Edyta A. Szurek, Anna Cebula, Vu L. Ngo, Maciej Pietrzak, Piotr Kraj, Timothy L. Denning, Leszek Ignatowicz

Biological Sciences Faculty Publications

The physiological role of T cell anergy induction as a key mechanism supporting self-tolerance remains undefined, and natural antigens that induce anergy are largely unknown. In this report, we used TCR sequencing to show that the recruitment of CD4+CD44+Foxp3−CD73+FR4+ anergic (Tan) cells expands the CD4+Foxp3+ (Tregs) repertoire. Next, we report that blockade in peripherally-induced Tregs (pTregs) formation due to mutation in CNS1 region of Foxp3 or chronic exposure to a selecting self-peptide result in an accumulation of Tan cells. Finally, we show that microbial antigens from Akkermansia muciniphila …

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Polar Bear Behavior: Morphologic And Physiologic Adaptations, John P. Whiteman Jan 2021

Polar Bear Behavior: Morphologic And Physiologic Adaptations, John P. Whiteman

Biological Sciences Faculty Publications

Polar bears possess morphologic and physiologic characteristics that reflect their terrestrial lineage as members of the bear family (Ursidae) as well as adaptations to the Arctic marine environment. Among marine mammals, they are the least adapted for aquatic life. They exhibit substantial seasonality in body mass, body condition, and many physiological functions, reflecting the annual cycle of both their Arctic sea ice habitat and the availability of their main prey, ringed seals. This hypercarnivorous diet has likely influenced the polar bear’s craniodental morphology and nutritional physiology. Similar to other marine mammal predators, polar bears exhibit a relatively high resting metabolic …

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Reproductive Biology Of The Spinner Shark Carcharhinus Brevipinna, Off The Southeast U.S. Coast, Kristin K. Palmrose Jan 2021

Reproductive Biology Of The Spinner Shark Carcharhinus Brevipinna, Off The Southeast U.S. Coast, Kristin K. Palmrose

UNF Graduate Theses and Dissertations

The spinner shark, Carcharhinus brevipinna, is a large coastal shark species that is common on the U.S. southeast coast and caught in both commercial and recreational fisheries. Little research has been conducted on the life history of C. brevipinna in the Northwestern Atlantic, presenting challenges for fishery management. This is especially true for reproductive biology, warranting a need to determine how rapidly individuals are reproducing and contributing to the population. This study aimed to characterize reproduction in C. brevipinna by determining size-at-maturity, reproductive seasonality, periodicity, and fecundity. This was accomplished by analyzing changes in reproductive tract morphology and histology, …

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Searching For Gold: Using A Novel Land Cover Classification To Identify Multiscale Drivers Of Site Occupancy By A Flagship Species For Early-Successional Habitat Conservation, Baron Lin Jan 2021

Searching For Gold: Using A Novel Land Cover Classification To Identify Multiscale Drivers Of Site Occupancy By A Flagship Species For Early-Successional Habitat Conservation, Baron Lin

Theses and Dissertations

Understanding habitat selection at multiple scales is an important step in guiding conservation programs and reversing species declines. This, however, is difficult for species that occupy early-successional habitats (ESH) due to a lack of accurate representation of shrub cover in publicly available land cover data. The Golden-winged Warbler (Vermivora chrysoptera; GWWA) is a threatened species of conservation concern and a flagship for ESH conservation. We used a novel, LIDAR-derived land cover classification that accurately identifies shrubs at a fine resolution (1m) to investigate how habitat composition and configuration influence GWWA site occupancy. We aggregated this same land cover …

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Impact Of Targeting Glycosylated Ceramides On Tumorigenic Properties, And Characterization Of Sphingolipids In Head And Neck Tumors, Katherine E. Hylton Jan 2021

Impact Of Targeting Glycosylated Ceramides On Tumorigenic Properties, And Characterization Of Sphingolipids In Head And Neck Tumors, Katherine E. Hylton

Theses and Dissertations

Sphingolipids play a crucial role in signaling, membrane structure, and migration, making them important molecules in cancer development, metastasis, and drug resistance. While some sphingolipids have been associated with pro-apoptotic or pro-survival behaviors, the role of many sphingolipids in cancer remains poorly defined. Chapter 1 of this study investigated the impact of three enzymes that compose most of the ceramide to Lc3 ceramide pathway – UGCG, GBA, and B3GNT5 – and how their alteration impacts chemoresistance and basal membrane invasion in vitro. It was found that the CΒE-induced inhibition of GBA, which converts glucosylceramide to ceramide, has no effects on …

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A Decision Tool To Identify Population Management Strategies For Common Ravens And Other Avian Predators, Andrea F. Currylow, Brenda J. Hanley, Kerry L. Holcomb, Timothy Shields, Stephen Boland, William I. Boarman, Mercy Vaughn Jan 2021

A Decision Tool To Identify Population Management Strategies For Common Ravens And Other Avian Predators, Andrea F. Currylow, Brenda J. Hanley, Kerry L. Holcomb, Timothy Shields, Stephen Boland, William I. Boarman, Mercy Vaughn

Human–Wildlife Interactions

Some avian species have developed the capacity to leverage resource subsidies associated with human manipulated landscapes to increase population densities in habitats with naturally low carrying capacities. Elevated corvid densities and new territory establishment have led to an unsustainable increase in depredation pressure on sympatric native wildlife prey populations as well as in crop damage. Yet, subsidized predator removal programs aimed at reducing densities are likely most effective longer-term when conducted in tandem with subsidy control, habitat management, and robust assessment monitoring programs. We developed decision support software that leverages stage structured Lefkovitch population matrices to compare and identify treatment …

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Predation Threat In A Variable Landscape: Connecting Predation Risk To Nesting Success For The Seaside Sparrow (Ammospiza Maritima Macgilivraii), Corina D. Newsome Jan 2021

Predation Threat In A Variable Landscape: Connecting Predation Risk To Nesting Success For The Seaside Sparrow (Ammospiza Maritima Macgilivraii), Corina D. Newsome

College of Graduate Studies: Theses & Dissertations

Predation, the leading cause of nest-failure in birds, not only exists as a direct threat to nesting success, but may exacerbate other sources of nest mortality. Birds inhabiting Atlantic coastal marshes, such as Seaside Sparrows (Ammospiza maritima; hereafter SESP), are experiencing significant and rapid changes to their habitat, particularly sea level rise (SLR) and encroaching urbanization, that may affect the relative influence of nest predation on overall productivity. For SESPs, SLR presents an inherent threat to nest success in its potential to increase the frequency of nest flooding. In addition to this direct threat, the ability of SESPs …

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Common Raven Impacts On Nesting Western Snowy Plovers: Integrating Management To Facilitate Species Recovery, Cheryl Strong, Kriss K. Neuman, Jenny L. Hutchinson, Jamie K. Miller, Amber L. Clark, Lena Chang, Joanna Iwanicha, Elizabeth Feucht, Matthew J. Lau, David J. Lauten, Sarah Markegard, Benjamin Pearl, David L. Sherer, Rachel Tertes, Susie Tharratt, Travis Wooten Jan 2021

Common Raven Impacts On Nesting Western Snowy Plovers: Integrating Management To Facilitate Species Recovery, Cheryl Strong, Kriss K. Neuman, Jenny L. Hutchinson, Jamie K. Miller, Amber L. Clark, Lena Chang, Joanna Iwanicha, Elizabeth Feucht, Matthew J. Lau, David J. Lauten, Sarah Markegard, Benjamin Pearl, David L. Sherer, Rachel Tertes, Susie Tharratt, Travis Wooten

Human–Wildlife Interactions

The U.S. Pacific coast population of the western snowy plover (Charadrius nivosus nivosus; plover) has declined due to loss and degradation of coastal habitats, predation, and anthropogenic disturbance. The U.S. Fish and Wildlife Service listed the subspecies in 1993 as threatened under the Endangered Species Act due to the population declines and habitat loss. Predation of nests and chicks has been identified as an important cause of historic population declines, and thus, most predator management actions for this subspecies are focused on reducing this pressure. In recent years, common ravens (Corvus corax; ravens) have become the most …

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Inter- And Intra-Annual Effects Of Lethal Removal On Common Raven Abundance In Nevada And California, Usa, Shawn T. O'Neil, Peter S. Coates, Julia C. Brockman, Pat J. Jackson, Jack O. Spencer Jr., Perry J. Williams Jan 2021

Inter- And Intra-Annual Effects Of Lethal Removal On Common Raven Abundance In Nevada And California, Usa, Shawn T. O'Neil, Peter S. Coates, Julia C. Brockman, Pat J. Jackson, Jack O. Spencer Jr., Perry J. Williams

Human–Wildlife Interactions

Populations of common ravens (Corvus corax; ravens) have increased rapidly within sagebrush (Artemisia spp.) ecosystems between 1960 and 2020. Although ravens are native to North America, their population densities have expanded to levels that negatively influence the population dynamics of other wildlife species of conservation concern, such as greater sage-grouse (Centrocercus urophasianus) and desert tortoises (Gopherus agassizii). For this reason, lethal removal, such as the application of the avicide DRC-1339, has been used to manage raven numbers at local scales and under certain circumstances. Because the relative effectiveness of DRC-1339 in reducing raven …

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Smart: A Science-Based Tiered Framework For Common Ravens, Seth J. Dettenmaier, Peter S. Coates, Cali L. Roth, Sarah C. Webster, Shawn T. O'Neil, Kerry L. Holcomb, John C. Tull, Pat J. Jackson Jan 2021

Smart: A Science-Based Tiered Framework For Common Ravens, Seth J. Dettenmaier, Peter S. Coates, Cali L. Roth, Sarah C. Webster, Shawn T. O'Neil, Kerry L. Holcomb, John C. Tull, Pat J. Jackson

Human–Wildlife Interactions

Large-scale increases and expansion of common raven (Corvus corax; raven) populations are occurring across much of North America, leading to increased negative consequences for livestock and agriculture, human health and safety, and sensitive species conservation. We describe a science-based adaptive management framework that incorporates recent quantitative analyses and mapping products for addressing areas with elevated raven numbers and minimizing potential adverse impacts to sensitive species, agricultural damage, and human safety. The framework comprises 5 steps: (1) desktop analysis; (2) field assessments; (3) comparison of raven density estimates to an ecological threshold (in terms of either density or density …

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Assessing The Utility Of Environmental Dna Techniques To Monitor White Shrimp (Litopenaeus Setiferus) Abundance On The Georgia Coast, Raven Hurt Jan 2021

Assessing The Utility Of Environmental Dna Techniques To Monitor White Shrimp (Litopenaeus Setiferus) Abundance On The Georgia Coast, Raven Hurt

College of Graduate Studies: Theses & Dissertations

Environmental DNA (eDNA) surveys have developed over the last decade from once being a novel tool to now acting as an effective technology often used in complement to traditional capture surveys for assessing the distribution of organisms in freshwater and marine environments. However, many uncertainties on how to properly develop, operate, and analyze eDNA based techniques still hinder this technology effectiveness in the field. The white shrimp, Litopenaeus setiferus, is a common, commercially and recreationally important species in the United States, with landings exceeding $254 million in 2020. L. setiferus is also used as a key indicator species to changes …

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Investigation Of The Efficacy Of Paclitaxel On Some Mirnas Profiles In Breast Cancer Stem Cells, Eli̇f Ertürk, Ferda Ari, Oğuzhan Akgün, Engi̇n Ulukaya, Cem İsmai̇l Küçükali̇, Şaki̇r Ümi̇t Zeybek Jan 2021

Investigation Of The Efficacy Of Paclitaxel On Some Mirnas Profiles In Breast Cancer Stem Cells, Eli̇f Ertürk, Ferda Ari, Oğuzhan Akgün, Engi̇n Ulukaya, Cem İsmai̇l Küçükali̇, Şaki̇r Ümi̇t Zeybek

Turkish Journal of Biology

Understanding of the functions of microRNAs in breast cancer and breast cancer stem cells have been a hope for the development of new molecular targeted therapies. Here, it is aimed to investigate the differences in the expression levels of let-7a, miR-10b, miR-21, miR-125b, miR-145, miR-155, miR-200c, miR-221, miR-222 and miR-335, which associated with gene and proteins in MCF-7 (parental) and MCF-7s (Mammosphere/stem cell-enriched population/CD44+/CD24-cells) cells treated with paclitaxel. MCF-7s were obtained from parental MCF-7 cells. Cytotoxic activity of paclitaxel was determined by ATP assay. Total RNA isolation and cDNA conversion were performed from the samples. Changes in expression levels of …

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Characteristics And Clinical Significance Of Cd163+/Cd206+M2 Mono-Macrophage In The Bladder Cancer Microenvironment, Xiangjie Kong, Ming Zhu, Zhirong Wang, Zhuoqun Xu, Jianfeng Shao Jan 2021

Characteristics And Clinical Significance Of Cd163+/Cd206+M2 Mono-Macrophage In The Bladder Cancer Microenvironment, Xiangjie Kong, Ming Zhu, Zhirong Wang, Zhuoqun Xu, Jianfeng Shao

Turkish Journal of Biology

The tumor microenvironment may recruit monocytes, with a protumoral macrophage phenotype (M2) that plays an important role in solid tumor progression and metastasis. Therefore, it is necessary to understand the characteristics of these cells for cancer prevention and treatment. Bladder cancer tissue samples and paracarcinoma tissues samples were collected, and the expression of CD163+ cells in tumor tissues was observed. Then, we observed the expression of infiltrating CD45+CD14+CD163+ cell subset and analyzed the molecular expressions related to immunity and angiogenesis. C57/BL6 mice were inoculated subcutaneously, and dynamic changes of CD11b+F4/80+CD206+ mononuclear macrophages expression for tumor-bearing mice were detected. The results …

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Investigation Of Neuro-Inflammatory Parameters In A Cuprizone Induced Mouse Model Of Multiple Sclerosis, Ti̇muçi̇n Avşar, Gökçe Erdem, Gökhan Terzi̇oğlu, Eda Tahi̇r Turanli Jan 2021

Investigation Of Neuro-Inflammatory Parameters In A Cuprizone Induced Mouse Model Of Multiple Sclerosis, Ti̇muçi̇n Avşar, Gökçe Erdem, Gökhan Terzi̇oğlu, Eda Tahi̇r Turanli

Turkish Journal of Biology

Cuprizone, copper chelator, treatment of mouse is a toxic model of multiple sclerosis (MS) in which oligodendrocyte death, demyelination and remyelination can be observed. Understanding T and B cell subset as well as their cytokines involved in MS pathogenesis still requires further scrutiny to better understand immune component of MS. The study presented here, aimed to evaluate relevant cytokines, lymphocytes, and gene expressions profiles during demyelination and remyelination in the cuprizone mouse model of MS. Eighty male C57BL/6J mice fed with 0.2% cuprizone for eight weeks. Cuprizone has been removed from the diet in the following eight weeks. Cuprizone treated …

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Classification Of Bladder Cancer Cell Lines According To Regulon Activity, Aleyna Eray, Serap Erkek Özhan Jan 2021

Classification Of Bladder Cancer Cell Lines According To Regulon Activity, Aleyna Eray, Serap Erkek Özhan

Turkish Journal of Biology

Bladder cancer is one of the most frequent cancers and causes more than 150.000 deaths each year. During the last decade, several studies provided important aspects about genomic characterization, consensus subgroup definition, and transcriptional regulation of bladder cancer. Still, much more research needs to be done to characterize molecular signatures of this cancer in depth. At this point, the use of bladder cancer cell lines is quite useful for the identification and test of new signatures. In this study, we classified the bladder cancer cell lines according to the activities of regulons implicated in the regulation of primary bladder tumors. …

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Fluid Shear Stress And Endothelial Cells Synergistically Promote Osteogenesis Of Mesenchymal Stem Cells Via Integrin Ss1-Fak-Erk1/2 Pathway, Mingli Jiang, Qihua Shen, Yi Zhou, Wenxia Ren, Miaomiao Chai, Yan Zhou, Wen-Song Tan Jan 2021

Fluid Shear Stress And Endothelial Cells Synergistically Promote Osteogenesis Of Mesenchymal Stem Cells Via Integrin Ss1-Fak-Erk1/2 Pathway, Mingli Jiang, Qihua Shen, Yi Zhou, Wenxia Ren, Miaomiao Chai, Yan Zhou, Wen-Song Tan

Turkish Journal of Biology

Prevascularization and mechanical stimulation have been reported as effective methods for the construction of functional bone tissue. However, their combined effects on osteogenic differentiation and its mechanism remain to be explored. Here, the effects of fluid shear stress (FSS) on osteogenic differentiation of rat bone-marrow-derived mesenchymal stem cells (BMSCs) when cocultured with human umbilical vein endothelial cells (HUVECs) were investigated, and underlying signaling mechanisms were further explored. FSS stimulation for 1-4 h/day increased alkaline phosphatase (ALP) activity and calcium deposition in coculture systems and promoted the proliferation of cocultured cells. FSS stimulation for 2 h/day was selected as the optimized …

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A Cytoplasmic Escapee: Desmin Is Going Nuclear, Ecem Kural Mangit, Ni̇lüfer Düz, Pervi̇n Ruki̇ye Di̇nçer Jan 2021

A Cytoplasmic Escapee: Desmin Is Going Nuclear, Ecem Kural Mangit, Ni̇lüfer Düz, Pervi̇n Ruki̇ye Di̇nçer

Turkish Journal of Biology

It has been a long time since researchers have focused on the cytoskeletal proteins' unconventional functions in the nucleus. Subcellular localization of a protein not only affects its functions but also determines the accessibility for cellular processes. Desmin is a muscle-specific, cytoplasmic intermediate filament protein, the cytoplasmic roles of which are defined. Yet, there is some evidence pointing out nuclear functions for desmin. In silico and wet lab analysis shows that desmin can enter and function in the nucleus. Furthermore, the candidate nuclear partners of desmin support the notion that desmin can serve as a transcriptional regulator inside the nucleus. …

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Neuropathy In Covid-19 Associated With Dysbiosis-Related Inflammation, Busra Aktas, Belma Aslim Jan 2021

Neuropathy In Covid-19 Associated With Dysbiosis-Related Inflammation, Busra Aktas, Belma Aslim

Turkish Journal of Biology

Although COVID-19 affects mainly lungs with a hyperactive and imbalanced immune response, gastrointestinal and neurological symptoms such as diarrhea and neuropathic pains have been described as well in patients with COVID-19. Studies indicate that gut-lung axis maintains host homeostasis and disease development with the association of immune system, and gut microbiota is involved in the COVID-19 severity in patients with extrapulmonary conditions. Gut microbiota dysbiosis impairs the gut permeability resulting in translocation of gut microbes and their metabolites into the circulatory system and induce systemic inflammation which, in turn, can affect distal organs such as the brain. Moreover, gut microbiota …

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The Current State Of Validated Small Molecules Inhibiting Sars-Cov-2 Non-Structural Proteins, Fati̇h Kocabaş, Merve Uslu Jan 2021

The Current State Of Validated Small Molecules Inhibiting Sars-Cov-2 Non-Structural Proteins, Fati̇h Kocabaş, Merve Uslu

Turkish Journal of Biology

The current COVID-19 outbreak has had a profound influence on public health and daily life. Despite all restrictions and vaccination programs, COVID-19 still can lead to fatality due to a lack of COVID-19-specific treatments. A number of studies have demonstrated the feasibility to develop therapeutics by targeting underlying components of the viral proteome. Here we reviewed recently developed and validated small molecule inhibitors of SARS-CoV-2's nonstructural proteins. We described the validation level of identified compounds specific for SARS-CoV-2 in the presence of in vitro and in vivo supporting data. The mechanisms of pharmacological activity, as well as approaches for developing …

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Determination Of The Interaction Between The Receptor Binding Domain Of 2019-Ncov Spike Protein, Tmprss2, Cathepsin B And Cathepsin L And Glycosidic And Aglycon Forms Of Some Flavonols, Erman Sali̇h İsti̇fli̇, Arzuhan Şihoğlu Tepe, Paulo Augusto Netz, Cengi̇z Sarikürkcü, İbrahi̇m Hali̇l Kiliç, Bektaş Tepe Jan 2021

Determination Of The Interaction Between The Receptor Binding Domain Of 2019-Ncov Spike Protein, Tmprss2, Cathepsin B And Cathepsin L And Glycosidic And Aglycon Forms Of Some Flavonols, Erman Sali̇h İsti̇fli̇, Arzuhan Şihoğlu Tepe, Paulo Augusto Netz, Cengi̇z Sarikürkcü, İbrahi̇m Hali̇l Kiliç, Bektaş Tepe

Turkish Journal of Biology

The novel coronavirus (COVID-19, SARS-CoV-2) is a rapidly spreading disease with a high mortality. In this research, the interactions between specific flavonols and the 2019-nCoV receptor binding domain (RBD), transmembrane protease, serine 2 (TMPRSS2), and cathepsins (CatB and CatL) were analyzed. According to the relative binding capacity index (RBCI) calculated based on the free energy of binding and calculated inhibition constants, it was determined that robinin (ROB) and gossypetin (GOS) were the most effective flavonols on all targets. While the binding free energy of ROB with the spike glycoprotein RBD, TMPRSS2, CatB, and CatL were -5.02, -7.57, -10.10, and -6.11 …

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Targeting Cov-2 Spike Rbd And Ace-2 Interaction With Flavonoids Of Anatolian Propolis By In Silico And In Vitro Studies In Terms Of Possible Covid-19 Therapeutics, Hali̇l İbrahi̇m Güler, Fulya Ay Şal, Zehra Can, Yakup Kara, Oktay Yildiz, Ali̇ Osman Beldüz, Sabri̇ye Çanakçi, Sevgi̇ Kolayli Jan 2021

Targeting Cov-2 Spike Rbd And Ace-2 Interaction With Flavonoids Of Anatolian Propolis By In Silico And In Vitro Studies In Terms Of Possible Covid-19 Therapeutics, Hali̇l İbrahi̇m Güler, Fulya Ay Şal, Zehra Can, Yakup Kara, Oktay Yildiz, Ali̇ Osman Beldüz, Sabri̇ye Çanakçi, Sevgi̇ Kolayli

Turkish Journal of Biology

Propolis is a multi-functional bee product rich in polyphenols. In this study, the inhibitory effect of Anatolian propolis against SARS-coronavirus-2 (SARS-CoV-2) was investigated in vitro and in silico. Raw and commercial propolis samples were used, and both samples were found to be rich in caffeic acid, p-coumaric acid, ferulic acid, t-cinnamic acid, hesperetin, chrysin, pinocembrin, and caffeic acid phenethyl ester (CAPE) at HPLC-UV analysis. Ethanolic propolis extracts (EPE) were used in the ELISA screening test against the spike S1 protein (SARS-CoV-2): ACE-2 interaction for in vitro study. The binding energy values of these polyphenols to the SARS-CoV-2 spike and ACE-2 …

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Coronavac (Sinovac) Covid-19 Vaccine-Induced Molecular Changes In Healthy Human Serum By Infrared Spectroscopy Coupled With Chemometrics, Ayça Doğan Mollaoğlu, Rafi̇g Gurbanov, Mete Severcan, Feri̇de Severcan Jan 2021

Coronavac (Sinovac) Covid-19 Vaccine-Induced Molecular Changes In Healthy Human Serum By Infrared Spectroscopy Coupled With Chemometrics, Ayça Doğan Mollaoğlu, Rafi̇g Gurbanov, Mete Severcan, Feri̇de Severcan

Turkish Journal of Biology

From the beginning of the COVID-19 coronavirus pandemic in December of 2019, the disease has infected millions of people worldwide and caused hundreds of thousands of deaths. Since then, several vaccines have been developed. One of those vaccines is inactivated CoronaVac-Sinovac COVID-19 vaccine. In this proof of concept study, we first aimed to determine CoronaVac-induced biomolecular changes in healthy human serum using infrared spectroscopy. Our second aim was to see whether the vaccinated group can be separated or not from the non-vaccinated group by applying chemometric techniques to spectral data. The results revealed that the vaccine administration induced significant changes …

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The Potential Use Of Drosophila As An In Vivo Model Organism For Covid-19-Related Research: A Review, Eşref Demi̇r Jan 2021

The Potential Use Of Drosophila As An In Vivo Model Organism For Covid-19-Related Research: A Review, Eşref Demi̇r

Turkish Journal of Biology

The world urgently needs effective antiviral approaches against emerging viruses, as shown by the coronavirus disease 2019 (COVID-19) pandemic, which has become an exponentially growing health crisis. Scientists from diverse backgrounds have directed their efforts towards identifying key features of SARS-CoV-2 and clinical manifestations of COVID-19 infection. Reports of more transmissible variants of SARS-CoV-2 also raise concerns over the possibility of an explosive trajectory of the pandemic, so scientific attention should focus on developing new weapons to help win the fight against coronaviruses that may undergo further mutations in the future. Drosophila melanogaster offers a powerful and potential in vivo …

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Cannabinoids For Sars-Cov-2 And Is There Evidence Of Their Therapeutic Efficacy?, Ahmet Onay, Abdulselam Ertaş, Veysel Süzerer, İsmai̇l Yener, Mustafa Abdullah Yilmaz, Emi̇ne Ayaz Ti̇lkat, Remzi̇ Eki̇nci̇, Nesri̇n Bozhan, Sevgi̇ İrtegün Jan 2021

Cannabinoids For Sars-Cov-2 And Is There Evidence Of Their Therapeutic Efficacy?, Ahmet Onay, Abdulselam Ertaş, Veysel Süzerer, İsmai̇l Yener, Mustafa Abdullah Yilmaz, Emi̇ne Ayaz Ti̇lkat, Remzi̇ Eki̇nci̇, Nesri̇n Bozhan, Sevgi̇ İrtegün

Turkish Journal of Biology

To combat the coronaviruses and their novel variants, therapeutic drugs and the development of vaccines that are to be effective throughout human life are urgently needed. The endocannabinoid system (ECS) acts as a modulator in the activation of the microcirculation, immune system, and autonomic nervous system, along with controlling pharmacological functions such as emotional responses, homeostasis, motor functions, cognition, and motivation. The ECS contains endogenous cannabinoids, cannabinoid receptor (CBRs), and enzymes that regulate their biosynthesis, transport, and degradation. Moreover, phytocannabinoids and synthetic cannabinoids that mimic the action of endocannabinoids also play an essential role in the modulation of the ECS. …

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Effects Of Bradykinin On Proliferation, Apoptosis, And Cycle Of Glomerular Mesangial Cells Via The Tgf-Ss1/Smad Signaling Pathway, Ji Dong, Li Ding, Liuwei Wang, Zijun Yang, Yulin Wang, Ying Zang, Xuexia Cao, Lin Tang Jan 2021

Effects Of Bradykinin On Proliferation, Apoptosis, And Cycle Of Glomerular Mesangial Cells Via The Tgf-Ss1/Smad Signaling Pathway, Ji Dong, Li Ding, Liuwei Wang, Zijun Yang, Yulin Wang, Ying Zang, Xuexia Cao, Lin Tang

Turkish Journal of Biology

We aimed to assess the effects of bradykinin (BK) on the proliferation, apoptosis, and cycle of glomerular mesangial cells via the transforming growth factor-ß 1 (TGF-ß1)/Smad signaling pathway. Rat glomerular mesangial cells, HBZY-1, were divided into normal group (untreated), model group (5 ng/L TGF-ß1), BK group (5 ng/L TGF-ß1 + 1 ng/L BK), and inhibitor group [5 ng/L TGF-ß1 + 1 ng/L LY2109761 (TGF-ß1-specific inhibitor)]. The cell proliferation, cycle, apoptosis, expression of type I collagen (Col-1), and protein expressions of Col-1, TGF-ß1, and phosphorylated Smad2 (p-Smad2) were detected by EdU labeling, flow cytometry, acridine orange/ethidium bromide (AO/EB) dual staining, immunofluorescence …

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Cellular Iron Storage And Trafficking Are Affected By Gtn Stimulation In Primary Glial And Meningeal Cell Culture, Lati̇fe Arzu Aral, Mehmet Ali̇ Ergün, Hayrunni̇sa Bolay Belen Jan 2021

Cellular Iron Storage And Trafficking Are Affected By Gtn Stimulation In Primary Glial And Meningeal Cell Culture, Lati̇fe Arzu Aral, Mehmet Ali̇ Ergün, Hayrunni̇sa Bolay Belen

Turkish Journal of Biology

A well-balanced intracellular iron trafficking in glial cells plays a role in homeostatic processes. Elevated intracellular iron triggers oxidative stress and cell damage in many neurological disorders, including migraine. This study aimed to investigate the effects of glyceryl trinitrate (GTN), on cellular iron homeostasis, matrixmetalloproteinase (MMP)-9, and calcitonin gene related peptide (CGRP) receptor (CRLR/CGRPR1) production in microglia, astrocyte, and meningeal cell cultures. Primary glial and meningeal cells in culture were exposed to GTN for 24 h. Messenger RNA expression was assessed using qPCR. Iron accumulation was visualized via modified Perl?s histochemistry. MMP-9 levels in cell culture supernatants were measured using …

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Current Mutatome Of Sars-Cov-2 In Turkey Reveals Mutations Of Interest, Doğa Eski̇er, Evren Akalp, Özlem Dalan, Gökhan Karakülah, Yavuz Oktay Jan 2021

Current Mutatome Of Sars-Cov-2 In Turkey Reveals Mutations Of Interest, Doğa Eski̇er, Evren Akalp, Özlem Dalan, Gökhan Karakülah, Yavuz Oktay

Turkish Journal of Biology

As the underlying pathogen for the COVID-19 pandemic that has affected tens of millions of lives worldwide, SARS-CoV-2 and its mutations are among the most urgent research topics worldwide. Mutations in the virus genome can complicate attempts at accurate testing or developing a working treatment for the disease. Furthermore, because the virus uses its own proteins to replicate its genome, rather than host proteins, mutations in the replication proteins can have cascading effects on the mutation load of the virus genome. Due to the global, rapidly developing nature of the COVID-19 pandemic, local demographics of the virus can be difficult …

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