Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Biology

Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 3991 - 4020 of 20626

Full-Text Articles in Entire DC Network

Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb Jan 2022

Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb

College of Graduate Studies: Theses & Dissertations

Nasonia is a parasitoid wasp genus that serves as an emerging model for studying speciation due to an incompatibility between nuclear and mitochondrial genomes between sister taxa. Short generation times, easy rearing in a lab setting, producing large amounts of progeny, and whole genome sequencing make Nasonia is an excellent candidate for studying incomplete reproductive isolation. Nasonia have five chromosomes and exhibit haplo-diploid sex determination in which fertilized eggs develop into diploid females and unfertilized eggs develop into haploid males. Recessive phenotypes are hidden in diploid females but are laid bare in haploid males as there is no interaction between …

Go to article

A Serological Analysis Of Sars-Cov-2 Infection In The Obstetric Population, Sophia Rose Jan 2022

A Serological Analysis Of Sars-Cov-2 Infection In The Obstetric Population, Sophia Rose

CMC Senior Theses

In December 2019, the surfacing and spread of a novel coronavirus, SARS-CoV-2, resulted in the global COVID-19 pandemic. As a viral antigen, SARS-CoV-2 poses a particular threat to the obstetric population due to physiological and immunological changes that women face during pregnancy. While recent studies have found that SARS-CoV-2 may have better clinical outcomes as compared to other betacoronaviruses, adverse pregnancy events such as ICU admission, preeclampsia, and/or preterm birth have been associated with COVID-19. Progress has been made in better understanding the pathophysiology of SARS-CoV-2 in pregnancy, but there is still much to be known about the interaction …

Go to article

Physician Assisted Dying (Pad): An Investigation Into The Mechanistic Action Of Opioid, Benzodiazepine, And Barbiturate Administration As An Alternative Measure To Forgoing Life Sustaining Treatment And Aggressive Palliation., Jordana Chanel Deighton Jan 2022

Physician Assisted Dying (Pad): An Investigation Into The Mechanistic Action Of Opioid, Benzodiazepine, And Barbiturate Administration As An Alternative Measure To Forgoing Life Sustaining Treatment And Aggressive Palliation., Jordana Chanel Deighton

CMC Senior Theses

Whilst modern medicine has increased longevity, the rise in life expectancy has brought about new struggles, namely that of aging and age related disease. Thus, humanity has been presented with a new problem: at what point is death preferable to aggressive life-prolonging treatment in the face of inevitable death? And if so, what right do individuals have to control the circumstances of their death? In the West, traditionally, individuals who seek to end their own lives in the face of terminal illness opt for forgoing artificial hydration and nutrition. Driven by an increase in desire for autonomous dignified death, a …

Go to article

Effects Of Drought On Habitat Quality For Native Bees In Residential Gardens Of Claremont, Ca, Max Proctor Jan 2022

Effects Of Drought On Habitat Quality For Native Bees In Residential Gardens Of Claremont, Ca, Max Proctor

CMC Senior Theses

Over 1,500 of the 4000 bee species found in North America inhabit California. Native bees are declining however, largely due to climate change and agricultural intensification. Previous research shows that cities can sustain diverse bee communities, due to the diversity of ornamental flowers. Urban green space represents an opportunity for native bee conservation. Residential gardens provide lots of green space and are urban pollinator hotspots. Managing yards for increased floral resources and nesting habitat can benefit native bee communities. Turfgrass provides few floral or nesting resources and negatively correlates with bee diversity. The 2011-2017 California drought caused many homeowners to …

Go to article

A Review Of Virus-Vectored Vaccines: Current Production Methods, Uses, Issues, And Future Perspectives, Jaden Marks Jan 2022

A Review Of Virus-Vectored Vaccines: Current Production Methods, Uses, Issues, And Future Perspectives, Jaden Marks

Biology and Microbiology Graduate Students Plan B Research Projects

Viral vector vaccines have recently risen to the forefront of vaccinology, but there are multiple factors that must be addressed before they achieve widespread approval and use. The concept of using viruses as vectors is not new, and numerous attempts to create vaccines that utilize virus vectors have been made in the past 25 years. Despite this, only one viral vector vaccine has gained full approval by the FDA. The goal of this review is to provide a comprehensive overview of the types of viral vector vaccines, production and purification methods, current and future viral vector vaccines, the issues and …

Go to article

Review Of A Chimeric Hemagglutinin-Based Influenza Vaccine Approach, Whitney Muck Jan 2022

Review Of A Chimeric Hemagglutinin-Based Influenza Vaccine Approach, Whitney Muck

Biology and Microbiology Graduate Students Plan B Research Projects

Influenza virus has affected human populations for centuries, causing an acute respiratory effect on the body. Even with scientific advancements in vaccine research, there are still serious limitations to the influenza vaccine. Varying strains and the constant recombination of the influenza virus has caused annual influenza circulation to be difficult to predict. The ongoing 40 race to develop a more efficient vaccine to broadly protect against the variety of influenza strains remains a public health priority. Many strategies have been studied to improve the current vaccine. The use of a chimeric hemagglutinin-based vaccine is in the first stages of human …

Go to article

The Relevance Of Pedigrees In The Conservation Genomics Era, Stephanie J. Galla, Julie A. Heath Jan 2022

The Relevance Of Pedigrees In The Conservation Genomics Era, Stephanie J. Galla, Julie A. Heath

Biology Faculty Publications and Presentations

Over the past 50 years conservation genetics has developed a substantive toolbox to inform species management. One of the most long-standing tools available to manage genetics—the pedigree—has been widely used to characterize diversity and maximize evolutionary potential in threatened populations. Now, with the ability to use high throughput sequencing to estimate relatedness, inbreeding, and genome-wide functional diversity, some have asked whether it is warranted for conservation biologists to continue collecting and collating pedigrees for species management. In this perspective, we argue that pedigrees remain a relevant tool, and when combined with genomic data, create an invaluable resource for conservation genomic …

Go to article

Chemosensitivity Of Locus Coeruleus Neurons Decreases With Postnatal Development, Yasmeen Samar Jan 2022

Chemosensitivity Of Locus Coeruleus Neurons Decreases With Postnatal Development, Yasmeen Samar

Browse all Theses and Dissertations

The locus coeruleus (LC) is a chemosensory area partially responsible for ventilation. Control over ventilation relies on inter-network communication via chemical and electrical synapses and chemosensitivity to CO2/pH. As the LC develops, it’s neural control over ventilation changes. This study investigates the chemosensitivity of the locus coeruleus over the course of development in postnatal rats. We hypothesize that 1) the CO2 sensitivity of LC neurons is highest at birth and declines with development 2) LC neurons are intrinsically chemosensitive and this chemosensitivity does not decrease with development and 3) the presence of gap junctions is highest at the beginning of …

Go to article

Cardiovascular Disease - Is A Whole Food Plant-Based Diet The Answer?, Shalom Katz Jan 2022

Cardiovascular Disease - Is A Whole Food Plant-Based Diet The Answer?, Shalom Katz

The Science Journal of the Lander College of Arts and Sciences

Objective: To determine if a whole food plant-based diet can prevent and cure cardiovascular disease. Results: People on whole food plant-based diets exhibit extremely low levels of plasma LDL. Another risk factor for developing atherosclerosis, is inhibited production of nitric oxide, which is vital for healthy blood flow. One of the main inhibitors of nitric oxide production is asymmetric dimethyl-l-arginine (ADMA). An inverse relationship was found between dietary fat and ADMA levels. The diets with the best results were the diets where oil intake was reduced to a minimum. Clinically, people who went on whole food plant-based diets had their …

Go to article

Inferring Phylogenetic Relationships Using The Smith-Waterman Algorithm And Hierarchical Clustering, Rafael Hidalgo, Anthony Devito, Nesreen Salah, Aparna S. Varde, Robert W. Meredith Jan 2022

Inferring Phylogenetic Relationships Using The Smith-Waterman Algorithm And Hierarchical Clustering, Rafael Hidalgo, Anthony Devito, Nesreen Salah, Aparna S. Varde, Robert W. Meredith

Department of Biology Faculty Scholarship and Creative Works

All biological species undergo change over time due to the evolutionary process. These changes can occur rapidly and unpredictably. Due to their high potential to spread quickly, it is critical to be able to monitor changes and detect viral variants. Phylogenetic trees serve as good methods to study evolutionary relationships. Complex big data in biomedicine is plentiful in regards to viral data. In this paper, we analyze phylogenetic trees with reference to viruses and conduct dynamic programming using the Smith-Waterman algorithm, followed by hierarchical clustering. This methodology constitutes an intelligent approach for data mining, paving the way for examining variations …

Go to article

Influences Of Grazing On Habitat Characteristics, Avian Community Composition And Nesting Bird Abundance Within Cheyenne Bottoms, Ks, Kirsten Granstrom-Arndt Jan 2022

Influences Of Grazing On Habitat Characteristics, Avian Community Composition And Nesting Bird Abundance Within Cheyenne Bottoms, Ks, Kirsten Granstrom-Arndt

Master's Theses or Doctor of Nursing Practice

Cheyenne Bottoms is a 41,000-acre prairie-marsh ecosystem in central Kansas. Approximately 8,000 acres of mixed grassland are dedicated for the conservation of bird populations, but little is known about the status of bird communities within these areas. This study took place within grassland areas of Cheyenne Bottoms from May – July 2021. I investigated bird community composition, relative abundance of frequently observed bird species, vegetative characteristics, and similarity of sites across different grazing intensities (continuous, rotational, and non-grazed). The four most common bird species observed were dickcissel (Spiza americana), grasshopper sparrow (Ammodramus savannarum), meadowlark species ( …

Go to article

A 16s & Its Soil Microbiome Analysis Of Native & Old-World Bluestem Invaded Soils Of Kansas Grasslands, Zachary Nelson Jan 2022

A 16s & Its Soil Microbiome Analysis Of Native & Old-World Bluestem Invaded Soils Of Kansas Grasslands, Zachary Nelson

Master's Theses or Doctor of Nursing Practice

Invasive species are becoming an increasing problem throughout the world. Their effect on local ecosystems is detrimental and widespread, harming productive efforts such as biomass accumulation, forcing native species (plant, animals, microbes) out, and ultimately, reducing biodiversity. Investigations of how invasive plants spread are widely studied; however, there has been little to no examination of how native plants influence the microbiome in the surrounding soil. In this study, I performed a fungal and bacterial metagenomic analysis of the soil and root microbiomes of both native grasslands and grasslands invaded by Old-World Bluestem Grasses to help determine if they influence the …

Go to article

Efficacy Of Non-Lethal Molecular Methods In Elucidating Distribution Of Gray Treefrog Complex (Hyla Chrysoscelis/Versicolor) In Kansas, Nora K. Lazerus Jan 2022

Efficacy Of Non-Lethal Molecular Methods In Elucidating Distribution Of Gray Treefrog Complex (Hyla Chrysoscelis/Versicolor) In Kansas, Nora K. Lazerus

Master's Theses or Doctor of Nursing Practice

Globally, amphibians are the most imperiled vertebrate taxa in part because they rely on both aquatic and terrestrial ecosystems. Specifically, their permeable skin makes them uniquely susceptible to habitat degradation and alteration. Cope’s Gray Treefrog (Hyla chrysoscelis) and the Gray Treefrog (Hyla versicolor) are a diploid-tetraploid, morphologically indistinguishable sister pair of cryptic anurans native to Kansas. Since 1987, the distribution of gray treefrogs in Kanas has extended west but the status of each species in the complex in Kansas is not known beyond its documented combined western expansion. Currently, species identification cannot be determined by nonlethal …

Go to article

Community-Level Effects Of A Widescale Reduction In White-Tailed Deer (Odocoileus Virginianus) Density, Mariah Slaughter Jan 2022

Community-Level Effects Of A Widescale Reduction In White-Tailed Deer (Odocoileus Virginianus) Density, Mariah Slaughter

Browse all Theses and Dissertations

The prolonged overabundance of white-tailed deer (Odocoileus virginianus) throughout the deciduous forests of eastern North America has resulted in widespread changes in the understory community. Studies have demonstrated that the exclusion of deer can allow some aspects of the understory to recover, but there are strong time-lags for deer-preferred species. Additionally, these exclosures only test the presence/absence of deer, which is not a feasible management option or desirable as deer are a part of the community. Here I evaluate how large-scale deer density manipulations within the Kinzua Quality Deer Cooperative of Pennsylvania affected understory vegetation communities. Using data from six …

Go to article

Rickettsial Pathogen Perturbs Tick Circadian Gene To Infect The Vertebrate Host, Supreet Khanal, Vikas Taank, John F. Anderson, Hameeda Sultana, Girish Neelakanta Jan 2022

Rickettsial Pathogen Perturbs Tick Circadian Gene To Infect The Vertebrate Host, Supreet Khanal, Vikas Taank, John F. Anderson, Hameeda Sultana, Girish Neelakanta

Biological Sciences Faculty Publications

Ixodes scapularis is a medically important tick that transmits several microbes to humans, including rickettsial pathogen Anaplasma phagocytophilum. In nature, these ticks encounter several abiotic factors including changes in temperature, humidity, and light. Many organisms use endogenously generated circadian pathways to encounter abiotic factors. In this study, we provide evidence for the first time to show that A. phagocytophilum modulates the arthropod circadian gene for its transmission to the vertebrate host. We noted a circadian oscillation in the expression of arthropod clock, bmal1, period and timeless genes when ticks or tick cells were exposed to alternate 12 h …

Go to article

Editorial: Tick Saliva: Secret To Blood Feeding Success, Daniel E. Sonenshine, Patricia A. Nuttall, Sukanya Narasimhan Jan 2022

Editorial: Tick Saliva: Secret To Blood Feeding Success, Daniel E. Sonenshine, Patricia A. Nuttall, Sukanya Narasimhan

Biological Sciences Faculty Publications

No abstract provided.

Go to article

Population Dynamics And Seasonal Movements Of Blue Suckers (Cycleptus Elongatus) In The James River, South Dakota, Tanner Leigh Carlson Jan 2022

Population Dynamics And Seasonal Movements Of Blue Suckers (Cycleptus Elongatus) In The James River, South Dakota, Tanner Leigh Carlson

Dissertations and Theses

Blue Suckers are a declining long lived catostomid, native to the large rivers and tributaries of the central United States. Currently in decline throughout their range due to anthropogenic impacts such as river channelization and dam construction, there are few stable Blue Sucker populations remaining. This study sought to describe population demographics of a stable population of Blue Suckers located in the unchannelized reach of the Missouri River below Gavins Point Dam. Year class strength, growth, and natural mortality was estimated based on aging data collected from lapillus otoliths. Previous research was conflicting as to what aging structure, pectoral fin …

Go to article

A Flora Of Coyote Ridge And Flat, Inyo County, California, Martin Purdy Jan 2022

A Flora Of Coyote Ridge And Flat, Inyo County, California, Martin Purdy

CGU Theses & Dissertations

California’s Sierra Nevada mountain range contains a disproportionate share of the state’s plant diversity and is one of the most floristically diverse regions of its size in the United States. The high Sierra Nevada, in particular, has been identified as an important center of species richness and endemism within California. Anthropogenic climate warming is expected to disproportionately affect mountain ecosystems, and models have predicted serious habitat contraction and extirpation for many alpine plant taxa. A specimen-based inventory of the vascular and non-vascular plants of Coyote Ridge and Flat was conducted to establish baseline data for one such sensitive alpine and …

Go to article

The Acute Physiological Response Of Polar Bears To Helicopter Capture, John P. Whiteman, Henry J. Harlow, George M. Durner, Eric V. Regehr, Steven C. Amstrup, Anthony M. Pagano, Merav Ben-David Jan 2022

The Acute Physiological Response Of Polar Bears To Helicopter Capture, John P. Whiteman, Henry J. Harlow, George M. Durner, Eric V. Regehr, Steven C. Amstrup, Anthony M. Pagano, Merav Ben-David

Biological Sciences Faculty Publications

Many wildlife species are live captured, sampled, and released; for polar bears (Ursus maritimus) capture often requires chemical immobilization via helicopter darting. Polar bears reduce their activity for approximately 4 days after capture, likely reflecting stress recovery. To better understand this stress, we quantified polar bear activity (via collar‐mounted accelerometers) and body temperature (via loggers in the body core [Tabd] and periphery [Tper]) during 2–6 months of natural behavior, and during helicopter recapture and immobilization. Recapture induced bouts of peak activity higher than those that occurred during natural behavior for 2 of 5 bears, …

Go to article

Benchmarking Ultra-High Molecular Weight Dna Preservation Methods For Long-Read And Long-Range Sequencing, Hollis A. Dahn, Jacquelyn Mountcastle, Jennifer Balacco, Sylke Winkler, Iliana Bista, Anthony D. Schmitt, Olga Vinnere Pettersson, Giulio Formenti, Karen Oliver, Michelle Smith, Wenhua Tan, Anne Kraus, Stephen Mac, Lisa M. Komoroske, Tanya Lama, Andrew J. Crawford, Robert W. Murphy, Samara Brown, Alan F. Scott, Phillip A. Morin, Erich D. Jarvis, Olivier Fedrigo Jan 2022

Benchmarking Ultra-High Molecular Weight Dna Preservation Methods For Long-Read And Long-Range Sequencing, Hollis A. Dahn, Jacquelyn Mountcastle, Jennifer Balacco, Sylke Winkler, Iliana Bista, Anthony D. Schmitt, Olga Vinnere Pettersson, Giulio Formenti, Karen Oliver, Michelle Smith, Wenhua Tan, Anne Kraus, Stephen Mac, Lisa M. Komoroske, Tanya Lama, Andrew J. Crawford, Robert W. Murphy, Samara Brown, Alan F. Scott, Phillip A. Morin, Erich D. Jarvis, Olivier Fedrigo

Biological Sciences: Faculty Publications

Background: Studies in vertebrate genomics require sampling from a broad range of tissue types, taxa, and localities. Recent advancements in long-read and long-range genome sequencing have made it possible to produce high-quality chromosome-level genome assemblies for almost any organism. However, adequate tissue preservation for the requisite ultra-high molecular weight DNA (uHMW DNA) remains a major challenge. Here we present a comparative study of preservation methods for field and laboratory tissue sampling, across vertebrate classes and different tissue types. Results: We find that storage temperature was the strongest predictor of uHMW fragment lengths. While immediate flash-freezing remains the sample preservation gold …

Go to article

Genome-Wide Identification Of Wrky Transcription Factor Encoding Genes In Amborella Trichopoda Baill. (Amborellaceae), Bijaya Pradhan Jan 2022

Genome-Wide Identification Of Wrky Transcription Factor Encoding Genes In Amborella Trichopoda Baill. (Amborellaceae), Bijaya Pradhan

Electronic Theses and Dissertations

WRKY transcription factors (TFs) are DNA-binding proteins, which play important roles in managing plant stresses and regulating growth and development processes. The WRKY TF family has expanded significantly through genome/gene duplications in the plant evolutionary process. Although the WRKY TFs have been identified in hundreds of plant species, they are yet to be identified in Amborella trichopoda, a species that represents the most basal group of Angiosperms. The main objectives of this study were to conduct genome-wide identification of the WRKY gene family in A. trichopoda and to assess their homologs in Angiosperms. In this study, we identified …

Go to article

Estrogen Regulation Of Daily Metabolic Rhythms In Female Mice, Oluwabukola Omotola Jan 2022

Estrogen Regulation Of Daily Metabolic Rhythms In Female Mice, Oluwabukola Omotola

Theses and Dissertations--Biology

Circadian rhythms are approximately 24-hour cycles of behavior, physiology, and gene expression. In mammals, these circadian rhythms are generated by clocks located in nearly every tissue in the body. The function of circadian clocks is to synchronize physiology and behavior with environmental cycles such as the light-dark cycle. After menopause, when circulating levels of estrogens are very low, women are more susceptible to obesity comorbidities such as metabolic syndrome and cardiovascular disease, suggesting that estrogens regulate these processes. Estrogens could protect females from metabolic dysfunction by regulating circadian rhythms. Consumption of diets that are high in fat contribute to obesity. …

Go to article

Activity Patterns Among Head-Started Juvenile Alligator Snapping Turtles (Macrochelys Temminckii), Parker R. Golliglee Jan 2022

Activity Patterns Among Head-Started Juvenile Alligator Snapping Turtles (Macrochelys Temminckii), Parker R. Golliglee

Graduate Theses/Dissertations

In contrast to many species of aquatic turtle, Alligator Snapping Turtles (Macrochelys temminckii) are putatively nocturnal. Data supporting this assertion are chiefly anecdotal, however, and two recent studies cast doubt on this generalization. Differences in activity patterns may be related to variability in temperature and photoperiod across the species’ range and may be influenced by ontogenetic changes, as well. To assess this, I equipped juvenile Alligator Snapping Turtles spanning a range of different ages with activity data loggers. I used 4-year-old turtles were to measure latitudinal differences in activity which were reared in hatchery ponds spanning the species’ …

Go to article

Largemouth Bass In The Upper Mississippi River: An Evaluation Of Management Strategies And Understanding Potential Factors Influencing Dynamic Rate Functions, Kylie Beth Sterling Jan 2022

Largemouth Bass In The Upper Mississippi River: An Evaluation Of Management Strategies And Understanding Potential Factors Influencing Dynamic Rate Functions, Kylie Beth Sterling

Graduate Theses/Dissertations

The Upper Mississippi River (UMR) supports ecologically and economically important commercial and recreational fisheries. One recreational fishery in the UMR is the Largemouth Bass fishery. Recreational fisheries can be effectively managed using information on population dynamics, though little is known about Largemouth Bass population dynamics in large river ecosystems. Therefore, the objectives of this study were to 1) evaluate recruitment, growth, and mortality of three Largemouth Bass populations in the UMR, specifically within Pools 4, 8, and 13, and 2) to use those estimates of recruitment, growth and mortality to inform exploitation models to evaluate best management practices for each …

Go to article

Investigation Of The Neuroprotective Effects Of Grape Seed Extract On Trigeminal Ganglion Primary Cultures, Sophia Rose Antonopoulos Jan 2022

Investigation Of The Neuroprotective Effects Of Grape Seed Extract On Trigeminal Ganglion Primary Cultures, Sophia Rose Antonopoulos

Graduate Theses/Dissertations

Migraine and temporomandibular disorders (TMD) are prevalent, debilitating orofacial pain conditions involving peripheral and central sensitization of the trigeminal system. The pro-inflammatory neuropeptide calcitonin gene-related peptide (CGRP), which is synthesized and secreted from trigeminal ganglion neurons, is implicated in the underlying pathology of migraine and TMD. Secreted CGRP modulates the excitability state of neurons and glial cells that express CGRP receptors. Recent studies from our lab in preclinical models of migraine and TMD have provided evidence that dietary supplementation with a proanthocyanin-enriched grape seed extract (GSE) inhibits trigeminal pain signaling. The effect of GSE was blocked by an antagonist of …

Go to article

Decomposition And Macroinvertebrate Shredder Colonization Of Autumnal Shed Sycamore Leaves In Mining-Contaminated Streams, Leslie Marie Hatch Jan 2022

Decomposition And Macroinvertebrate Shredder Colonization Of Autumnal Shed Sycamore Leaves In Mining-Contaminated Streams, Leslie Marie Hatch

Graduate Theses/Dissertations

Leaf decomposition in streams is an important ecological function facilitated by bacteria, fungi, and macroinvertebrates. Metal contamination can decrease leaf decomposition rates and reduce macroinvertebrate abundance and diversity. In the current study, I focused on mining-contamination in well-buffered streams in two different Missouri mining districts with varying extents of mining contamination, Big River and Pierson Creek. I measured decomposition rates of sycamore leaves, and abundance and diversity of macroinvertebrate shredders in leaf pack experiments with a full-factorial design to determine the effects of metal contamination of leaves and stream substrates. Comparisons were made between leaf packs upstream and downstream of …

Go to article

Environmental Effects On Biomass Allocation And Small Plot Evaluations Of Aquatic Pesticides For Control Of Nitellopsis Obtusa (Starry Stonewort) Collected From Lake Koronis In Stearns County, Minnesota, Patrick J. Carver Jan 2022

Environmental Effects On Biomass Allocation And Small Plot Evaluations Of Aquatic Pesticides For Control Of Nitellopsis Obtusa (Starry Stonewort) Collected From Lake Koronis In Stearns County, Minnesota, Patrick J. Carver

All Graduate Theses, Dissertations, and Other Capstone Projects

Phenology is the study of critical life stages in plants in relation to shifts in environmental factors during seasonal changes. Starry stonewort is a green macro alga in the family Characeae native to Eurasia. Much of the Midwestern United States has been invaded by this species. Starry stonewort has been seen to have late season growth patterns in its invaded range. This study found that this growth pattern was dependent upon water temperature and light transmittance both of which affect biomass production and senescence. It was hypothesized that peak biomass would occur in the late summer (August), however we observed …

Go to article

Sheepshead (Archosargus Probatocephalus) Neurocranium, Andrew J. Hartmann, Steven Peterson, David W. Kerstetter Jan 2022

Sheepshead (Archosargus Probatocephalus) Neurocranium, Andrew J. Hartmann, Steven Peterson, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from Sheepshead (Archosargus probatocephalus)

Go to article

Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi Jan 2022

Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi

Electronic Theses and Dissertations

Globally, canola is the second largest oilseed crop after soybean, which is processed for the commercial production of high value oil. The industrial processing steps for canola oil extraction include preprocessing (cleaning, pressing, flaking, and cooking), mechanical pressing, and/or organic solvents. This process results in large quantities of protein-rich (~ 40% dry basis) meal as a co-product. The meal is used partially in animal diet (<30% inclusion) due to high levels of antinutritional factors (ANFs) such as high fibers, phytic acid, and glucosinolates, and low metabolizable energy. Thus, this research was designed to examine various bioprocessing technologies (i.e., traditional sprouting, solid state fermentation, submerged state fermentation, co-culture fermentation, mild pretreatments, and their combinations) for possible advancement in canola use. Canola seed sprouting for 6-day period led to an increase in protein content and a reduction in ANFs and oil content of sprouts compared to ungerminated seed. Subsequent submerged state fermentation of 6- day old sprouts using three different strains (Aureobasidium pullulans, Trichoderma reesei and Neurospora crassa) further increased protein content and lowered ANFs. Solid-state fermentation of 144 h old sprouts using A. pullulans, N. crassa and T. reesei enhanced the protein content and reduced ANFs of sprouts. Sprouting canola seed for three days helped in hull removal, leading to high protein meal accompanied by low fiber and phytic acid level. Three-day seed sprouting had no effect on oil yield, but free fatty acid content was higher compared to seed oil. Co-culture fermentation of HECM under solid state process showed maximum reduction in fiber content with co-culture of A. pullulans and N. crassa while a combination of A. pullulans and T. reesei promoted the highest GLS and phytic acid reductions compared to other combinations, which indicated the advantage of coculture inoculation over monoculture in terms of ANFs reduction. Mild pretreatment of HECM using deionized water resulted in washed HECM (WHECM) with lower soluble sugars and GLS compared to untreated HECM, whereas protein and amino acid were concentrated due to the removal of soluble components. WHECM compared to HECM showed higher protein digestibility when fed to rainbow trout. Subsequent mono- and coculture fermentation of HECM and WHECM under submerged process resulted in higher protein and amino acid content and lower ANFs levels compared to uninoculated controls. The results of cellulase, endoglucanase and β-glucosidase activity indicated the crucial role of used substrates, fungi, fermentation modes (solid state/submerged stated) and inoculation methods (mono-/co-culture) on enzyme activities.

Go to article

Evolutionary Conservation Of The Dream Subcomplex Muvb, Spencer Snider Jan 2022

Evolutionary Conservation Of The Dream Subcomplex Muvb, Spencer Snider

Dissertations, Master's Theses and Master's Reports

As the publications of annotated genomes from species representing most domains of life continue to grow exponentially, we are gaining more insight into how proteins, cellular pathways, and protein complexes evolved. We are interested in understanding how each protein in the 8-subunit transcriptional repressor complex called DREAM interacts with each other. DREAM is comprised of 3 main components: an E2F-DP transcription factor heterodimer, a pocket protein, and the highly conserved 5-subunit subcomplex called MuvB. We hypothesize that the mechanism of DREAM’s formation on chromatin dictates how DREAM functions to turn off target gene expression. Unfortunately, many interaction surfaces remain unknown, …

Go to article

First Prev Next Last