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Geographic Variation In Blue Orchard Bee (Osmia Lignaria) Development And Performance As A Managed Pollinator In The Western United States, Morgan B. Scalici

All Graduate Theses and Dissertations, Fall 2023 to Present

Most flowering plants, including many cultivated food crops, will only produce well-developed fruits and seeds if pollen is transferred from one flower to another with the help of an animal pollinator. Honey bees are the most well-known and ubiquitous but are poor pollinators of some commercially important crops, or are in poor supply during crop bloom. In such cases, farmers will employ other managed pollinators such as bumble bees or solitary bees like mason and leafcutting bees. The blue orchard bee is North America's most agriculturally important native mason bee as effective pollinators of spring-blooming fruit crops. Differences in developmental …

The Impacts Of Maturation And Experience On Volumetric Neuroplasticity In Solitary And Social Bees, Mallory A. Hagadorn

All Graduate Theses and Dissertations, Fall 2023 to Present

Some animals are incredibly social, living and working together as one cohesive group. Alternatively, many animals are solitary, never living with and rarely interacting with others. A large body of biological research has focused on understanding the role that brains play in promoting these behavioral differences across species. Even so, it remains unclear why some brains facilitate social behavior while others do not. My dissertation aims to advance our understanding of this concept by characterizing bees’ brains and how they change over a lifetime. Bees are beneficial for investigating relationships between the brain and social behavior because some species are …

Phenology Of The Invasive Balsam Woolly Adelgid, Adelges Piceae (Ratz.) (Hemiptera: Adelgidae), On Subalpine Fir In Northern Utah, Elizabeth L. Rideout

All Graduate Theses and Dissertations, Fall 2023 to Present

Balsam woolly adelgid (BWA) is an invasive true fir pest in North America. Native to Europe, BWA was first discovered in Utah attacking subalpine fir in 2017. Recent BWA-caused subalpine fir mortality in northern Utah has prompted the need for baseline biological research to support pest management. Small-bodied and blending easily with its environment, BWA is a challenging pest to detect and study. Phenology, or the timing and characteristics of life stages through the year, of BWA varies depending on elevation and climate and is unstudied in Utah. This research focuses on defining aspects of BWA’s phenology, including the number …

The Mojave Poppy Bee (Perdita Meconis) And A Primary Plant Host, The Las Vegas Bear Poppy (Arctomecon Californica): Status And Interactions, Sarit Chanprame

All Graduate Theses and Dissertations, Fall 2023 to Present

The Mojave Desert is one of the most biodiverse places in North America. A harsh environment with many species results in complex relationships between animals and plants. This study focuses on one bee-plant relationship, the Mojave poppy bee, and the Las Vegas Bear Poppy.

The Mojave poppy bee is rare and has only been collected from Las Vegas Bear Poppy, Dwarf Bear Poppy, and a few species of prickly poppies. A 1995 report suggested the bee was a major contributor of pollination for the Las Vegas Bear Poppy in Clark County, Nevada. More recently (2019), their local extinction in southwestern …

Activity Patterns Of Whiptail Lizards (Aspidoscelis) Found In The Northern Chihuahuan Desert, Guillermo Alvarez

Open Access Theses & Dissertations

Traditional lizard pitfall traps were modified to allow organisms to escape, while collecting valuable ecological data. Modified camera traps were capable of detecting the same species as traditional traps in a semi-arid environment, without posing the associated mortality risks. Pitfall-camera traps were used to sample the activity of the Side-blotched Lizard (Uta stansburiana) and the Southwestern Fence Lizard (Sceloporus cowlesi) in an urban wetland during four continuous years. Evaluations on activity pattern and the activity overlap between the two species revealed extensive overlap, with minor but significant seasonal shifts mediating coexistence. Traps were also effective at detecting other reptiles, invertebrates, …

The Role Of The Cholesterol Synthesis Pathway In Primitive Erythropoiesis, Jose Hernandez

Open Access Theses & Dissertations

Erythropoiesis is the process by which new red blood cells (RBCs) are formed and defects in this process can lead to anemia or thalassemia. The GATA1 transcription factor is an established mediator of RBC development. However, the upstream mechanisms that regulate the expression of GATA1 are not completely characterized. Cholesterol is 1 potential upstream mediator of GATA1 expression because previously published studies suggest that defects in cholesterol synthesis disrupt RBC differentiation. Here we characterize RBC development in a zebrafish harboring a single missense mutation in the hmgcs1 gene (Vu57 allele). Hmgcs1 encodes the first enzyme in the cholesterol synthesis pathway …

Repositioning Propranolol To Block Mitogenic Signaling In Breast Cancer, Alexa Noel Montoya

Open Access Theses & Dissertations

Breast cancer is the second most diagnosed cancer among women and is about 30% of all new cases of female cancers each year. It is projected that 1 in 8 every U.S. woman (about 13%) develop invasive breast cancer over the course of her lifetime. While advances in cancer research have made it possible to elucidate several breast cancer genomic subtypes, and develop new novel therapies, many of these agents are associated with significant toxicity, as well as high costs. A retrospective cross-sectional study of 404 breast cancer patients was performed to determine the effect of β-blocker usage on tumor …

Lizard Communities In A Shifting Landscape: The Current State Of Insectivorous Lizard Communities In The Jornada Basin Between Grassland, Mesquite Dune, And Creosote Bush Habitats In A Semi-Arid Landscape Undergoing Shrubification, Julie Marie Schlichte

Open Access Theses & Dissertations

Lizard communities in arid lands can act as model systems of how diversity and abundance are impacted in the face of ongoing climate variability and change, as they are sensitive to shifts in precipitation, temperature, and changing vegetation structure. The main research question we hope to answer is how the community composition of lizards varies between dominant vegetation types (Mesquite dunes, grasslands, and Creosote Bush flats). Nine sites (3 of each dominant vegetation type) with 16 pitfall traps each (n = 144) were surveyed from June 2016 -- June 2017. 12 lizard species were captured, consisting of 923 total captures …

Assessing Risks For Bovine And Zoonotic Tuberculosis Through Spatial Analysis And A Questionnaire Survey In Fiji – A Pilot Study, Jenny-Ann L. M. L. Toribio, Keresi Lomata, Sam Fullman, Aaron Jenkins, Elva Borja, Shumaila Arif, Jarrad Mckercher, David Blake, Anabel Garcia, Richard J. Whittington, Frank Underwood, Ben J. Marais

Research outputs 2022 to 2026

Mycobacterium bovis causes tuberculosis in cattle and when transmitted to humans typically causes extra-pulmonary tuberculosis (EPTB). Bovine tuberculosis (bTB) has a global distribution and is controlled in most countries to protect animal and public health. Recent studies revealed that bTB is established on dairy farms in Fiji where EPTB cases have been reported in people. The aims of this pilot investigation were to look for putative zoonotic TB (EPTB) cases in people and to evaluate practices that might contribute to the persistence and transmission of M. bovis between cattle and to humans. Existing data sets were shared between the Fiji …

Trehalose Enhances Mitochondria Deficits In Human Npc1 Mutant Fibroblasts But Disrupts Mouse Purkinje Cell Dendritic Growth Ex Vivo., Collin M Macleod, Fawad A K Yousufzai, Liam T Spencer, Sarah Kim, Lucianne A Rivera-Rosario, Zerian D Barrera, Lindsay Walsh, Claude Krummenacher, Benjamin Carone, Ileana Soto

College of Science & Mathematics Departmental Research

Lysosomes play important roles in catabolism, nutrient sensing, metabolic signaling, and homeostasis. NPC1 deficiency disrupts lysosomal function by inducing cholesterol accumulation that leads to early neurodegeneration in Niemann-Pick type C (NPC) disease. Mitochondria pathology and deficits in NPC1 deficient cells are associated with impaired lysosomal proteolysis and metabolic signaling. It is thought that activation of the transcription factor TFEB, an inducer of lysosome biogenesis, restores lysosomal-autophagy activity in lysosomal storage disorders. Here, we investigated the effect of trehalose, a TFEB activator, in the mitochondria pathology of NPC1 mutant fibroblasts in vitro and in mouse developmental Purkinje cells ex vivo. We …

Lionfish (Pterois Volitans) Neurocranium, Kate Heffner, Hosanna Gattle, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 30 cm total length (TL) Lionfish (Pterois volitans), collected from Second Reef on 15/10/2023 by Kate Heffner.

Yellowtail Snapper Ocyurus Chrysurus, Kevin Rafferty, Manuel Ploner, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

YELLOWTAIL SNAPPER OCYURUS CHRYSURUS 7.5CM Total length

The Neuronal Calcium Sensor Ncs-1 Regulates The Phosphorylation State And Activity Of The Gα Chaperone And Gef Ric-8a, Daniel Muñoz-Reyes, Levi J. Mcclelland, Sandra Arroyo-Urea, Sonia Sánchez-Yepes, Juan Sabín, Sara Pérez-Suárez, Margarita Menendez, Alicia Mansilla, Javier García-Nafría, Stephen Sprang, Maria Jose Sanchez-Barrena

Biological Sciences Faculty Publications

The neuronal calcium sensor 1 (NCS-1), an EF-hand Ca binding protein, and Ric-8A coregulate synapse number and probability of neurotransmitter release. Recently, the structures of Ric-8A bound to Gα have revealed how Ric-8A phosphorylation promotes Gα recognition and activity as a chaperone and guanine nucleotide exchange factor. However, the molecular mechanism by which NCS-1 regulates Ric-8A activity and its interaction with Gα subunits is not well understood. Given the interest in the NCS-1/Ric-8A complex as a therapeutic target in nervous system disorders, it is necessary to shed light on this molecular mechanism of action at atomic level. We have reconstituted …

Yellow Edge Grouper (Hyporthodus Flavolimbatus) Neurocranium, Lili Mcelhill, Alison Novara, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium (11 cm) of Yellow Edge Grouper (Hyporthodus flavolimbatus), collected from South Florida local fish market on 02/11/2023 by Alison Novara and Lili McElhill.

Antibiotic Resistance At Sequence, Population, And Global Scales, Kevin Blake

Arts & Sciences Graduate Student Theses and Dissertations

Antibiotic resistance (AR) can be studied at multiple scales. The smallest is at the level of the DNA or amino acid sequence of AR genes, characterizing how AR genes are encoded, regulated, and expressed. Next is the level of protein structure, deciphering the 3D shape of AR proteins and how that shape affects function. The next higher scale is that of biochemistry, studying the kinetics of these proteins’ mechanism of action and interactions with drug molecules. The level of populations studies individual species of bacteria that are resistant to specific antibiotics. The next higher scale, of microbiomes, examines many species …

Jackfish (Caranx Lugubris) Neurocranium, Lauren Groves, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 9 cm total length (TL) Jackfish, Caranx lugubris, collected from Ideal Market on September 18th, by Lauren Groves

Brha, Kevin Rafferty, Briana K. Zaffiro, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Skull from Broad-winged Hawk (Buteo platypterus).

Northern Red Snapper (Lutjanus Campechnus) Neurocranium, Ajani K. Saldana, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 7.5 cm total length (TL) 37 cm Lutjanus campechanus (Northern Red Snapper), collected from Mango Tree Supermarket in September 2023 by Ajani Saldana

Blsk 3d Scan, Kevin Rafferty, Briana K. Zaffiro, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Skull from Black Skimmer (Rhnchops niger)

Ictalurus Punctatus (Channel Catfish) Neurocranium, Kayla D. Jones, Maura P. Wondra, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 9.70 cm total length (TL) Ictalurus punctatus (Channel Catfish), collected from Davie, Florida in June 2023 by Katrina Sawickij.

Changes In Fecal Glucocorticoid Metabolites In Captive Coyotes (Canis Latrans): Influence Of Gender, Time, And Reproductive Status, Eric M. Gese, Patricia A. Terletzky, Cole A. Bleke, Erika T. Stevenson, Susannah S. French

Wildland Resources Faculty Publications

Reproduction is considered an energetically and physiologically demanding time in the life of an animal. Changes in physiological stress are partly reflected in changes in glucocorticoid metabolites and can be measured from fecal samples. We examined levels of fecal glucocorticoid metabolites (fGCMs) in 24 captive coyotes (Canis latrans) to investigate responses to the demands of reproduction. Using 12 pairs of coyotes (five pairs produced pups, seven pairs did not), we analyzed 633 fecal samples covering 11 biological periods (e.g., breeding, gestation, and lactation). Levels of fGCMs showed high individual variability, with females having higher fGCM levels than males. …

[Caranx Hippos (Jack Crevalle)] Neurocranium, Liat N. Castillo, Dylan J. Seiffert, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from [80cm TL] Caranx hippos (Jack Crevalle), collected from Merrit Island, Florida [9/18/2023] by Dylan Seiffert.

In Vivo Volumetric Depth-Resolved Imaging Of Cilia Metachronal Waves Using Dynamic Optical Coherence Tomography, Tian Xia, Kohei Umezu, Deirdre M Scully, Shang Wang, Irina V Larina

Faculty, Staff and Students Publications

Motile cilia are dynamic hair-like structures covering epithelial surfaces in multiple organs. The periodic coordinated beating of cilia creates waves propagating along the surface, known as the metachronal waves, which transport fluids and mucus along the epithelium. Motile ciliopathies result from disrupted coordinated cilia beating and are associated with serious clinical complications, including reproductive disorders. Despite the recognized clinical significance, research of cilia dynamics is extremely limited. Here, we present quantitative imaging of cilia metachronal waves volumetrically through tissue layers using dynamic optical coherence tomography (OCT). Our method relies on spatiotemporal mapping of the phase of intensity fluctuations in OCT …

Keynote Address: Seasonal Changes In The Avian Community Of The Wright State Woods, Jeffrey L. Peters

Runkle Woods Symposia

Dr. Jeffrey Peters is a Professor of Biological Sciences at Wright State University. He earned his B.S. in Biology at Indiana University of Pennsylvania. For his M.S. in Applied Ecology and Conservation Biology at Frostburg State University in Maryland, Dr. Peters used DNA analyses to examine mating strategies in a species of duck, the Gadwall. Continuing his genetics research, he studied geographic variation in ducks for his Ph.D. in Biology at the University of Maryland, Baltimore County. Dr. Peters continued this work at the University of Alaska, Fairbanks, as a post-doctoral researcher, and at Wright State University. During his career, …

The Insult Of Road Salt, Rebecca N. Tuttle, Benson Sparkman, Landon Shackleford, Audrey E. Mcgowin

Runkle Woods Symposia

Wright State students Abby Tuttle, Benson Sparkman, and Landon Shackleford present their undergraduate research on the high chloride levels in the Wright State Woods, it's soil and water, caused by the use of winter road salt.

Pawpaws: Ohio’S State Fruit, Don Cipollini

Runkle Woods Symposia

Dr. Don Cipollini presents his episode on Pawpaws from his show The Naturalist followed by a Q&A.

Dr. Cipollini takes us on a walk through the Wright State University Woods and his own backyard to talk about Ohio's state fruit, the Pawpaw and the trees that grow it. He discusses how to grow pawpaw, the biology of the tree, and how you can use and eat the fruit it produces.

Economics Of Conservation And The Application To The Runkle Woods, Indigenous American Cultural Student Association, Brad Kerry, Eliza Hendrix, Mateo Bush, Ryan Diza

Runkle Woods Symposia

Student Eliza Hendrix presents a brief history of local Native American culture to give context to Adjunct Professor Brad Kerry's portion of the presentation focusing on conservation through an economic lens.

A Novel Subcluster Of Closely Related Bacillus Phages With Distinct Tail Fiber/Lysin Gene Combinations, Rachel E. Loney, Veronique A. Delesalle, Brianne E. Chaudhry, Megan Czerpak, Alexandra A. Guffey, Leo Goubet-Mccall, Michael Mccarty, Madison S. Strine, Natalie T. Tanke, Albert C. Vill, Gregory P. Krukonis

Biology Faculty Publications

Bacteriophages (phages) are the most numerous entities on Earth, but we have only scratched the surface of describing phage diversity. We isolated seven Bacillus subtilis phages from desert soil in the southwest United States and then sequenced and characterized their genomes. Comparative analyses revealed high nucleotide and amino acid similarity between these seven phages, which constitute a novel subcluster. Interestingly, the tail fiber and lysin genes of these phages seem to come from different origins and carry out slightly different functions. These genes were likely acquired by this subcluster of phages via horizontal gene transfer. In conjunction with host range …

A Snapshot Into The Lives Of Elephants: Camera Traps And Conservation In Etosha National Park, Namibia, Jodie L. Berezin, Amanda J. Odom, Virginia Hayssen, Caitlin E. O'Connell-Rodwell

Biological Sciences: Faculty Publications

Knowledge of elephant movement and grouping patterns in the wild is critical for their management and conservation. Much of these data come from GPS collar data and aerial surveys, which have provided invaluable information, but data from these methods are often limited to small groups or entire populations. Effective elephant management requires both generalized and localized methodologies. Here, we propose the expanded use of camera traps in research relating to elephant localized movements and grouping patterns as an additional tool for elephant conservation management. In this study, we use a battery-powered camera trap to provide daily high-resolution data of African …

Channel Catfish (Ictalurus Punctatus), Neurocranium, Corinne Renshaw, Brianna Buckley, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 4.9 cm total length 20.6 cm Ictalurus punctatus (Channel Catfish), collected from Dr. Kerstetter's dissection specimens.