Digital Commons Network^™

Purification, Characterization, And Submitochondrial Localization Of The 32-Kilodalton Nadh Dehydrogenase From Maize, Andrew F. Knudten, Jay J. Thelen, Michael Hans Luethy, Thomas Elthon

School of Biological Sciences: Faculty Publications

Plant mitochondria have the unique ability to directly oxidize exogenous NAD(P)H. We recently separated two NAD(P)H dehydrogenase activities from maize (Zea mays 1.) mitochondria using anion-exchange (Mono Q) chromatography. The first peak of activity oxidized only NADH, whereas the second oxidized both NADH and NADPH. In this paper we describe the purification of the first peak of activity to a 32-kD protein. Polyclonal antibodies to the 32-kD protein were used to show that it was present in mitochondria from several plant species. Two-dimensional gel analysis of the 32-kD NADH dehydrogenase indicated that it consisted of two major and one minor …

The Effect Of High Intensity Exercise Training Verses Low Intensity Exercise Training On Fractionated Plasma High-Density Lipoprotein Cholesterol, Teresa L. Spate

Masters Theses

Exercise training is thought to result in an elevation of high-density lipoprotein (HDL) and the cardioprotective subfraction HDL{dollar}\sb2{dollar}. Twenty-five healthy women (39 {dollar}\pm{dollar} 7.8 yrs; 70 {dollar}\pm{dollar} 14 kg) walked two miles three times per week for 12 weeks to examine the effect of exercise intensity on the HDL profile. The L.I. group (N = 12) walked at 60% of the heart rate reserve (HRR) and the H.I. group (N = 13) walked at 80% HRR both maintaining the prescribed distance and frequency. A 22% increase in total HDL (from 32 {dollar}\pm{dollar} 6 to 39 {dollar}\pm{dollar} 8 mg/dl) and a …

Identification Of Tire Leachate Toxicants And A Risk Assessment Of Water Quality Effects Using Tire Reefs In Canals, S. M. Nelson, G. Mueller, D. C. Hemphill, U.S. Bureau Of Reclamation

Publications (WR)

Cover is an important component of aquatic habitat and fisheries management. Fisheries biologists often try to improve habitats through the addition of natural and artificial material to improve cover diversity and complexity. Habitat-improvement programs range from submerging used Christmas trees to more complex programs using sophisticated artificial habitat modules. Used automobile tires have been employed in the large scale construction of reefs and fish attractors in marine environments and to a lesser extent in freshwater and have been recognized as a durable, inexpensive and long-lasting material which benefits fishery communities.

Recent studies by the U.S. Bureau of Reclamation have quantified …

Terrapene Carolina Carolina, Joseph C. Mitchell, Rafael O. De Sá

Biology Faculty Publications

TERRAPENE CAROLINA CAROLINA (Eastern Box Turtle). REPRODUCTION. Female Terrapene carolina containing oviductal eggs have been recorded in Virginia from 26 May to 25 July (Mitchell, in press. The Reptiles of Virginia. Smithsonian Inst. Press, Washington, D.C.). Other reports of reproduction in Terrapene (see list in Ernst and McBreen 1991. Cat. Amer. Amphib. Rept. 512.1-512.13) indicate that nesting occurs in late spring and early summer throughout its range.

Per-Like Immunoreactive Neurons Within The Brain Of Xenopus Innervate Both The Retina And Pineal Organ, J. M. Garcia-Fernandez, Kathleen King Siwicki, R. G. Foster

Biology Faculty Works

No abstract provided.

Innervation Patterns In Neuromasts Of The Mottled Sculpin, Cottus Bairdi, Jie He

Master's Theses

No abstract provided.

Morphometry Of Growth In Hatchling Striped Plateau Lizards (Iguanidae: Sceloporus Virgatus), Geoffrey R. Smith

Faculty Publications

No abstract provided.

Susceptibility Of Eumeces Species Skinks To A Southeastern Isolate Of The Lyme Disease Spirochete (Borrelia Burgdorferi), Gregory A. Cummins

College of Graduate Studies: Theses & Dissertations (1964–2006)

The SI-4 strain of Borrelia burgdorferi obtained from field collected Ixodes scapularis on Sapelo Island, Georgia, was infective to seven of eight cotton mice (Peromyscus gossypinus) by needle inoculation. I. scapularis larvae were then fed on them, and nymphs molting from these larvae were then fed on laboratory mice, Mus musculus, in an attempt to transmit the spirochete. One of three mice was successfully infected; confirmation of the presence of B. burgdorferi was made using BSK II culture and the polymerase chain reaction (PGR). A subsequent attempt was made to determine if SI-4 was infective for Eumeces …

The Role Of Sexual Selection In Structuring West Indian Passerine Communities, C. Lynne Starling

College of Graduate Studies: Theses & Dissertations (1964–2006)

Patterns of plumage dimorphism were examined among species of passerines native to 19 West Indian islands. Based on sexual selection theory, the predictions were made that dimorphic species: (1) occur on fewer and larger islands than monomorphic species due to reduced colonization ability and reduced survivorship imposed by sexual selection pressure, and (2) should be added to island communities disproportionately as area increases, an idea which was tested using linear models.

No significant difference was observed between number of islands or minimum island area occupied by dimorphics versus monomorphics. The traditional ANCOVA model best fits the data and suggests that …

Toward An Understanding Of Alzheimer's Disease: The Effects Of B-Amyloid(1-42) And Ibotenic Acid On The Retention Of A Spatial Learning Task In Rats F:Ollowing Multiple Injections Into The Hippocampus, Jason Pequette '94

Honors Projects

Neuropathologically, Alzheimer's disease (AD) is characterized by neuritic plaques and neurofibrillary tangles. Evidence has suggested that a protein called B-amyloid (BA) is a major component of the neuritic plaques and may playa role in the neurodegeneration seen in AD. The cellular mechanisms by which BA induces neurotoxicity, however, are still unclear. Recent evidence suggests that the aggregational state of BA may be relevant to its neurotoxicity. Whether portions of the BA protein or the entire sequence produces neurotoxicity in neurons, however, remains a controversy. Still another controversy is whether BA is directly neurotoxic to neurons or whether it increases the …

Quantitative Elemental Mapping Of Biomedical Specimens Using The Nuclear Microprobe., Jan Pallon, John Knox

Scanning Microscopy

Quantitative and structural elemental information is available from the nuclear microprobe through a time consuming fit of the (X-ray) spectrum at each point. An alternative technique is proposed which greatly shortens the analysis procedure and allows an increased number of samples to be processed. The method discussed here is to create elemental maps which, when they are divided by the charge/pixel and multiplied by a scaling factor, will form quantitative maps. The scaling factors are obtained from a calibration procedure comparing a large number of fitted X-ray spectra with the corresponding contents of selected energy windows. The technique also allows …

Ischemia/Reperfusion Injury Of The Ascending Colon In Ponies: A Correlative Study Utilizing Microvascular Histopathology And Corrosion Casting, B. J. Darien, P. A. Sims, W. C. Stone, D. R. Schilly, R. R. Dubielzig, R. M. Albrecht

Scanning Microscopy

Volvulus of the ascending colon (ACV) in the horse results in microvascular injury and necrosis of the intestinal mucosa. This study investigated the site and type of microvascular injury which occurs within the mucosa and sub mucosa following ACV. Histopathology of volvulus treated ponies demonstrated mucosa! necrosis with microvascular hemorrhage and thrombosis. Thrombi occurred within the subepithelial capillaries and edema and hemorrhage developed throughout the mucosa and submucosa. Vascular casts allowed 3-D viewing of samples obtained from the entire pelvic flexure and demonstrated two distinct microvascular changes: 1) disruption of the colonic glandular capillary network occurred concomitantly with the …

Effects Of Modulated And Continuous Microwave Irradiation On Pyroantimonate Precipitable Calcium Content In Junctional Complex Of Mouse Small Intestine, Z. Somosy, G. Thuróczy, J. Kovács

Scanning Microscopy

The pyroantimonate precipitable calcium content of intestinal epithelial cells was investigated in mice following total body irradiation with 2450 MHz continuous and low frequency (16 Hz) square modulated waves. In the control animals the reaction products appeared in the intercellular space of adjacent cells including intermediate junctions and desmosomes and were absent in the area of tight junctions. Immediately after low frequency modulated microwave irradiation at 0.5 and 1mW/cm² power densities, a rapid distribution of pyroantimonate precipitable calcium content was observed. The pyroantimonate deposits were located on the cytoplasmic side of lateral membrane, in the area of junctional complex, …

Intracellular And Extracellular Elemental Composition Of The Endolymphatic Sac Studied By X-Ray Microanalysis, Romuald Wroblewski

Scanning Microscopy

X-ray microanalysis was performed along with light microscopy (LM) on rapidly frozen and cryo-sectioned endolymphatic sac tissues of adult guinea pigs, to determine the elemental composition of the different cell types in this tissue as well as the content of the sac lumen. The morphological preservation and spatial resolution of cryo-sectioned endolymphatic sac was found adequate for the identification of the different cell types of the sac in the transmission electron microscope. Further cell type identification was performed by comparing scanning transmission electron microscopy images with LM images on adjacent serial sections. X-ray microanalysis demonstrated differences between epithelial and sub-epithelial …

Clock Control Of Circadian Changes Of Ommatidial Structure In The Cockroach, Leucophaea Maderae (L.), Zhuming Zhang

Masters Theses & Specialist Projects

All eukaryotic organisms display rhythms which persist under constant environmental conditions with periods of approximately, but very seldom exactly, 24 hours. Such rhythms are "circadian" and are driven by an internal "biological clock." Circadian rhythms of locomotor activity, retinal sensitivity to light and ommatidial morphology have been characterized in the cockroach, Leucophaea maderae (L.). It is not known whether the same clock(s) controls both circadian rhythms of electroretinogram (ERG) amplitude and morphological changes of the compound eye. In order to determine whether the location of the clock that controls morphological changes is in the same location as the one that …

Characterization Of Bartonella-Bacilliformis Flagella And Effect Of Antiflagellin Antibodies On Invasion Of Human Erythrocytes, David C. Scherer, Isaure Deburon-Connors, Michael F. Minnick

Biological Sciences Faculty Publications

Bartonella bacilliformis is the etiologic agent of Oroya fever in humans. Flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. To address this hypothesis, we purified and characterized flagella from strain KC584 and then assessed their role in human erythrocyte association and invasion. Electron microscopy of the flagellar preparation showed a high concentration of filaments with a mean wavelength of 800 nm. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblot analysis, and KBr density gradient centrifugation indicated that the flagellar filament is composed of a polypeptide of 42 kDa. The flagellin is partially (ca. 50%) resistant …

In Vivo & In Vitro Effects Of Light On Ommatidial Morphology In The Cockroach, Leucophaea Maderae, Channon Yule

Masters Theses & Specialist Projects

Clock-driven endogenous circadian rhythms in ommatidial morphology have been reported in Leucophaea maderae. The parameters used to measure changes in ommatidial morphology over time were palisade layer and rhabdom areas and the organization of lightscreening pigment granules about the rhabdom. The fact that the palisade layer area only partially returned to the light-adapted state in vivo during subjective midday was indicative of a passive mechanism, not involving the clock output. Complete return of the palisade layer area to the light-adapted state during the daytime in animals held under LD 12:12 indicated light might play an active role. Temporal changes …

The Mayfly Newsletter, Peter M. Grant

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Electrophysiological Properties Of Identified Classes Of Lamprey Spinal Neurons, James T. Buchanan

Biological Sciences Faculty Research and Publications

1. As part of a continuing analysis of the mechanisms of the central pattern generator underlying fictive swimming in lamprey, a systematic survey of electrophysiological properties of lamprey neurons was made in the in vitro spinal cord preparation with the use of intracellular current-clamp recordings. A total of 70 neurons was included in the study, representing 6 classes of spinal neurons. The classes were myotomal motoneurons, three classes of interneurons involved in fictive swimming [lateral interneurons, nerve cells with contralateral and caudal projecting axons (CC interneurons), and excitatory interneurons], and two classes of interneurons involved in sensory processes (edge cells …

Complete Nucleotide Sequence Of Psti-L Fragment Of Tanapox Virus Genomic Dna, Sarah J. Neering

Masters Theses

Tanapox virus (TPV), of the genus Yatapoxvirus, is a human poxvirus that was originally isolated from the Tana River Valley in Kenya in 1962. TPV produces a mild disease characterized by a short febrile illness associated with one or more nodular skin lesions. Although there have been limited studies on TPV, other poxviruses such as vaccinia virus, have been extensively characterized and their genomic DNAs sequenced. In order to examine the molecular features of TPV, we have cloned approximately 17% of the genomic DNA. TPV DNA was digested with Pstl restriction endonuclease and cloned into pUC19. The Pst …

Xenopus Laevis As A Model Organism, David C. Cannatella, Rafael O. De Sá

Biology Faculty Publications

Model organisms are often assumed to be representative of some more inclusive taxon of which the species is a part. This assumption leads to mistaken generalizations about the evolutionary and comparative significance of the data gathered. This paper reviews com? parative and evolutionary studies of Xenopus laevis and its relatives. Phylogenetic analysis of data from DNA sequences and morphology indicate that Xenopus is monophyletic and that Silurana is its sister group. The most basal lineages of Pipidae diverged prior to the breakup of Gondwana. The bizarre morphology of Xenopus is in part due to changes in the mode of meta? …

Migration Of Epithelium During Phenytoin-Dependent Gingival Overgrowth In Mice, S. H. Ashrafi, B. Atassi, R. Erickson, T. Sabet

Scanning Microscopy

A small cavity was made in the mesiopalatal area of the maxillary first molar adjacent to the gingiva. Mice were maintained on 40 mg/kg phenytoin (or on diluent for control) by daily intraperitoneal injections. After 9 weeks, light microscopic observations revealed that in experimental mice, epithelial cells migrated towards the cavity and covered it. In controls, epithelial cell migration towards the cavity did not occur. For scanning electron microscopic (SEM) studies, specimens were fixed in 4% glutaraldehyde in 0.1 M phosphate buffer, pH 7.2, for 2 hours, dehydrated, critical point dried and coated with gold. The surface of the outer …

Body Size In Amblyrhiza Inundata (Rodentia, Caviomorpha), An Extinct Megafaunal Rodent From The Anguilla Bank, West Indies: Estimates And Implications, Audrone R. Biknevicius, Donald A. Mcfarlane, Ross D. E. Macphee

WM Keck Science Faculty Papers

Rodent species typically evolve larger mean body sizes when isolated on islands, but the extinct caviomorph Amblyrhiza inundata, known only from Quaternary cave deposits on the islands of Anguilla and St. Martin (northern Lesser Antilles), provides an unusually dramatic example of insular gigantism. Here we report on a series of body mass estimates for Amblyrhiza using predictive equations based on anteroposterior diameters and cortical cross-sectional areas of humeral and femoral diaphyses. Analyses of 14 isolated specimens (5 femoral, 9 humeral), all representing adult or near adult animals, yield body mass estimates ranging from slightly less than 50 kg to more …

Proceedings Of The Charter Boat Management Workshop Held As Part Of The First National Fisheries Manager Conference Terrigal Nsw 21 September 1993, A. E. Magee

Fisheries Management Papers

General concern of all states was the actual AND potential impact of charter fishing on the resource and the inability to measure such impacts. The perceived problem with dual licensing was unregulated effort and the abilit to circumvent management controls. This paper provides current charter boat management arrangements in Australia, why they should be managed, types of charter vessels, Licensing, Management of recreational only charter vessels, Non-consumptive charters, Management guides and small scale charter operators, Commonwealth jurisdiction, Development of agreed strategies and Identification of future needs.

Bone As A Spring, Frank E. Fish

Biology Faculty Publications

No abstract provided.

Reduction Of Sunburn Damage To Skin By Topical Application Of Vitamin E Acetate Following Exposure To Ultraviolet B Radiation: Effect Of Delaying Application Or Of Reducing Concentration Of Vitamin E Acetate Applied, John R. Trevithick, David T. Shum, Sahle Redae, Kenneth P. Mitton, Christopher Norley, Stephen J. Karlik, Alan C. Groom, E. E. Schmidt

Scanning Microscopy

The skin of the skh-1 mouse after ultraviolet B (280-320 nm, UVB) irradiation shows the pathological changes typical of sunburn damage: spongiosis (edematous spaces) around some cells, necrosis of keratinocytes, giving rise to sunburn cells, inflammatory infiltration ofpolymorphonuclear leucocytes, etc. In our previous study, these were accompanied by erythema, increased skin sensitivity, and edematous swelling. The topical application of tocopherol acetate (TA) immediately after the UVB exposure decreased these changes. In this paper, multiple measurements of the skin thickness were made at different locations along the magnetic resonance imaging (MRI) cross-sectional image of the skin. This permits effects to be …

Biological Inventory, Natural History, And Human Impact Of Palo Alto National Battlefield, Norman L. Richard, Alfred T. Richardson

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

During 1993 a floral and fauna inventory was conducted on the Palo Alto National Battlefield Historic Site . Vegetational associations were mapped, and the impacts of agriculture and ranching were assessed. Attention was given to identifying endangered, threatened, or candidate species present within the site boundaries, and also species which may have been extirpated. An attempt has been made to envision where historic roadways were located and how the 1846 landscape may have appeared.

Review Of Recreational Gill, Haul And Cast Netting In Western Australia., F. B. Prokop

Fisheries Management Papers

In this paper the Recreational Netting Committee has addressed an extremely complex and controversial issue in a forthright manner. They have developed a set of management proposals which accommodates recreational netting while recognizing the concerns of non-netters and the general community. The Committee would like to stress that it considers this plan as a package. For example the Committee would be strongly opposed to the considerable operational restrictions contained under Section 5 if there were not a trade-off in terms of a fairer criteria for which estuaries are open as proposed in Section 6.2. Similarly, the Committee would not be …

Management Arrangements For The Southern Demersal Gillnet And Demersal Longline Fishery 1994/95 Season., Southern Demersal Gillnet And Demersal Longline Management Advisory Committee

Fisheries Management Papers

At its meeting of 7 April 1993 the Southern Demersal Gillnet and Demersal Longline Fishery Management Advisory Committee (hereafter called "the Committee) was advised of the Western Australian Fisheries Department's Research Division's concern over the status of shark stocks. All the data available indicate that whiskery sharks are severely over-exploited with stock levels declining by around 10% to 30% of original levels. Current levels of fishing effort are expected to cause the stock to decline further. Gummy sharks have high risk of over exploitation (stock has declined to 40% -50% of original levels. Dusty whaler stocks (bronze whaler) is uncertain …

A Simple Empirical Calibration Of Energy Dispersive X-Ray Analysis (Edxa) On The Cornea, N. F. Schrage, K. Benz, P. Beaujean, W. -G. Burchard, M. Reim

Scanning Microscopy

Monitoring of the corneal electrolyte content is important for the study of chemical eye burns. This paper describes quantitative measurements on gelatin standards, corneas and a cornea homogenate with an energy dispersive X-ray analyzer (EDX) in the scanning electron microscope (SEM). Ten micrometers thick cryosections were freeze-dried and mounted on solid carbon supports. The applied quantification procedure was a local peak background analysis with a specifically designed computer program. Similar chemical and physical properties of gelatin, cornea homogenate, and cornea were proven by EDX-analysis and wet chemical analysis. Gelatin standards with known concentrations of different added salts showed linear correlations …