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Demonstration Of The Fine Structure Of Stereocilia In The Organ Of Corti Of The Guinea Pig By Field Emission Scanning Electron Microscopy, W. L. Jongebloed, E. A. Dunnebier, F. W. J. Albers, D. Kalicharan

Scanning Microscopy

A combined perfusion- and immersion prefixation with glutaraldehyde followed by a tannic acid/arginine/osmium tetroxide (TAO) treatment of the guinea pig cochlea is described for field-emission gun scanning electron microscopy (FEG-SEM) observation of the fine structure of the stereocilia of the organ of Corti. Conventional osmium tetroxide postfixation methods in combination with a thin conductive coating failed to show the fine structure of the glycocalyx of the epithelial lining in the endolymphatic compartment of the cochlea, in particular, on the stereocilia surface. The antennulae-like glycocalyx covering of the stereocilia surface of the more pronounced rows of outer hair cells has been …

Neural Transplant Staining With Dii And Vital Imaging By 2-Photon Laser-Scanning Microscopy, Steve M. Potter, Jerome Pine, Scott E. Fraser

Scanning Microscopy

We are developing a multielectrode silicon "neuroprobe" for maintaining a long-term, specific, two-way electrical interface with nervous tissue. Our approach involves trapping a neuron (from an embryonic rat hippocampus) in a small well with a stimulation/ recording electrode at its base. The well is covered with a grillwork through which the neuron's processes are allowed to grow, making synaptic contact with the host tissue, in our case a cultured slice from a rat hippocampus. Each neuroprobe can accommodate 15 neurons, one per well. As a first step in studying neurite outgrowth from the neuroprobe, it was necessary to develop new …

Diffuse Neuroendocrine System: Structural And Functional Effects Of Radiation Injury To Amine Precursor Uptake And Decarboxylation (Apud) Cells, I. M. Kvetnoy, V. V. Yuzhakov, A. O. Molotkov, L. N. Bandurko, R. A. Brodsky, N. D. Yakovleva

Scanning Microscopy

The paper presents a review of the results obtained by the authors on the study of external (gamma) and internal (I-131) radiation effects on the functional morphology and linkage of the diffuse neuroendocrine system (DNES) and amine precursor uptake and decarboxylation (APUD) cells of the stomach and duodenum. The investigations performed enabled us to determine that the morphological changes noted in APUD cells had a dose and time dependency. The present study supports the point of view that the radiation initiates serotonin release from APUD cells, which appears to initiate the mechanism of early postirradiation dysfunctions of the gastrointestinal tract …

Nitrogen Transformations In A Mitigated Wetland In The Green Bottom Wildlife Management Area, Mark Allen Fisher

Theses, Dissertations and Capstones

Soil nitrogen (N) dynamics have been shown to be quite sensitive to the soil hydrology. Creation of a mitigation freshwater wetland from an old field provided an opportunity to examine changes in N dynamics in terrestrial, aquatic (but formerly terrestrial), and transition (seasonally inundated) soils.

This thesis determined N availability using in situ incubations of soils taken from three distinct habitat conditions based on the degree of inundation following wetland creation: (1) old field (no inundation), (2) transition (seasonally inundated, initially beneath 10-20 cm of water), and (3) mitigation wetland (permanently inundated). Sample plots were located along five parallel transects …

Geographic Variation Of S̲O̲R̲E̲X̲ C̲I̲N̲E̲R̲E̲U̲S̲ In West Virginia, Lisa Jane Gatens

Theses, Dissertations and Capstones

The morphological and geographical diversity of Sorex cinereus is evident in West Virginia. In an attempt to identify patterns of morphological variation relative to age, sex, and geographic locality, and to clarify taxonomic status of the West Virginia soricids by defining diagnostic characters applicable to this area, a sample of 288 specimens representing three taxa were analyzed statistically. Standard external measurements were recorded, and a series of 12 cranial and dental characters were measured and recorded for each specimen. Morphological variation due to age was found in S. c. cinereus, but not in the smaller reference samples of S. …

Use Of The (3)H-Tetracycline Rat Model In The Study Of Skeletal Compartmentalization And Metabolism Of Calcium In The Maturing Male And Female Rat And The Role Of The Skeleton In Calcium Homeostasis In The Adolescent Male Rat, Darrin Lee Demoss

Theses, Dissertations and Capstones

The (3)H-tetracycline bone labeling procedure was employed to monitor bone resorption from urinary loss curves in male and female rats of various ages. In addition, whole body dry skeletal mass and the loss of (3)H-tetracycline from individual bones was determined. It was found that the dry skeletal mass/body mass ratio of 24-week-old females was 30-40% greater than that for males. The urinary loss of (3)H-tetracycline was described by a double exponential equation indicating the presence of two distinct and independent exchangeable bone compartments. Both compartments decrease in size with age, but their label loss activities were different. The label loss …

Dietary Overview Of Hemidactylus Turcicus With Possible Implications Of Food Partitioning, Daniel Saenz

Faculty Publications

A stomach content analysis was conducted on Hemidactylus turcicus, the Mediterranean gecko, from 19 April 1990 to 15 October 1990, on the campus of Stephen F. Austin State University, Nacogdoches, Texas. Geckos (N = 167) were placed into four size groups based on snout-vent-length (5 29 mm, 30-39 mm, 40-49 mm, and z 50 mm) and three microhabitat groups based on perch height (those occupying perch sites < 1.52 m, 2 1.52 m to 5 3.05 m, and > 3.05 m). Stomach contents were analyzed and sex, size, and microhabitat groups were compared using Schoener's percent overlap index. Volumetrically, the most important prey items taken were Orthoptera, Lepidoptera, and Isopoda. Geckos of …

Effect Of Larval Swimming Duration On Success Of Metamorphosis And Size Of The Ancestrular Lophophore In Bugula Neritina (Bryozoa), Dean E. Wendt

Biological Sciences

There is a growing realization that events during one portion of an organism's life cycle can have both subtle and dramatic effects on other stages in the life history. Lethal and sublethal effects associated with the duration of larval swimming in marine invertebrates were examined for the bryozoan Bugula neritina. Larvae were kept swimming up to a maximum of 28 h at 20{deg}C by exposure to continuous bright fluorescent illumination. At 4-h intervals, samples of 20-40 larvae were removed from bright illumination and were exposed to seawater containing 10 mM excess KCI, an inducer of metamorphosis in this species. …

Analysing Ancient Dna, Raúl J. Cano

Biological Sciences

Much of what we know about extinct organisms comes from traits that are not preserved in the fossil record. Until recently, morphological analysis was the only tool available for scientists to determine relationships for extinct fossil organisms. We now know that ‘ancient’ DNA can be preserved in the remains of extinct organisms. By targeting specific gene sequences, it may be possible to deduce biochemical characteristics and through sequence comparisons, to estimate the extent of evolutionary divergence. By comparing the amount and type of these changes, one could estimate how quickly some DNA ‘evolves’ relative to other segments, or which genes …

Organic Carbon, Hydrogen, And Nitrogen Concentrations In Surficial Sediments From Western Long Island Sound, Connecticut And New York, Roman Zajac, Larry J. Poppe, Ralph S. Lewis, S E. Pratt, Zofia J. Mlodzinska, B. B. Taylor, Mary L. Digiacomo-Cohen

Biology and Environmental Science Faculty Publications

Total organic carbon, hydrogen, and nitrogen (CHN) analyses were performed on 147 surficial sediment samples from study areas off the Norwalk Islands and Milford, Connecticut, in western Long Island Sound. The CHN data and gross lithologic descriptions of the sediments are reported herein. The concentrations of total organic carbon (TOC), hydrogen, and nitrogen in these samples average 1.54, 1.40, and 0.17 weight percent, respectively. The individual CHN concentrations vary inversely with grain size, with CHN values increasing with the percent fines. Increasing nutrient inputs and decreasing circulation cause TOC and nitrogen values to generally increase westward within the Sound. C/N …

The Effect Of The Mammalian Hormone Estrogen On The Yeast C̲A̲N̲D̲I̲D̲A̲ A̲L̲B̲I̲C̲A̲N̲S̲, Marcia Clifton Finucane

Theses, Dissertations and Capstones

Changes in the balance between commensal microbes and mammalian hosts can cause disease if the scales tip in favor of the microbe. Although usually caused by a defective host immune response, overgrowth of or changes in the microbe may also be responsible for opportunistic infections. Clinical and laboratory investigations have indicated that mammalian hormones, including estrogen, can influence the production of symptomatic disease and the virulence attributes of Candida albicans.

This study demonstrated enhanced growth of some strains of C. albicans when 17β-estradiol was added to the growth medium. Measurement of extracellular protein under the influence of estradiol did …

Characterization Of A Basolateral Electroneutral Na+/H+ Antiporter In Atlantic Lobster (Homarus Americanus) Hepatopancreatic Epithelial Vesicles, Jeff Duerr, Gregory A. Ahearn

Faculty Publications - Department of Biological & Molecular Science

Purified basolateral membrane vesicles (BLMVs) were prepared from Atlantic lobster (Homarus americanus) hepatopancreas using a Percoll density gradient technique. Enrichments of the Na+/K+-ATPase and alkaline phosphatase activities of these vesicles were 15.4- and 1.2- fold, respectively. The presence of amiloride-sensitive Na+/H+ exchange was demonstrated. Contrary to electrogenic 2Na+/1H+ exchange on apical membranes from the same tissue, kinetic studies of Na+ transport by these basolateral membranes indicate an electroneutral antiport with a Km of 28±1.7 mmol l21 and a Jmax of 1.74±0.13 mmol mg21 min21. Amiloride interacted at a single binding site (Ki=39mmol l21) and external Li+ was shown to be …

Neotropical Bird Migration During The Ice Ages: Orientation And Ecology, Timothy C. Williams , '64, T. Webb Iii

Biology Faculty Works

Reconstruction of breeding habitat of North American Neotropical migrants 18,000 years ago and 9,000 years ago indicated major shifts in both location and composition of plant communities relative to present conditions. Increased vegetation in xeric areas may have compensated, at least in part, for the reduction in breeding habitat due to glaciation. Autumnal flights of Neotropical passerine migrants flying on constant headings from North America to Central and South America were simulated under present wind conditions and for winds during periods of glaciation at 18,000 and 9,000 years ago. The 155 degrees average headings currently observed for Atlantic migrants were …

Top-Down Cascade From A Bitrophic Predator In An Old-Field Community, Matthew D. Moran, Thomas P. Rooney, L. E. Hurd

Biological Sciences Faculty Publications

We tested the hypothesis that a bitrophic (third and fourth level) arthropod predator can exert a cascading, top-down influence on other arthropods and plants in an early successional old field. First-stadium mantids, Tenodera sinensis, were added to replicated open-field plots in numbers corresponding to naturally occurring egg hatch density and allowed to remain for ≈ 2 mo. Sticky-trap dispersal barriers around both control and mantid-addition plots allowed us to monitor emigration of arthropods continuously during the experiment. Biomass of herbivores, carnivores, and plants, and abundances of arthropod taxa within plots were determined at the beginning, middle, and end of …

Post-Conflict Behavior In Japanese Macaques At The Indianapolis Zoo: Age Of Opponents Influences Reconciliation, Scott Kight

Department of Biology Faculty Scholarship and Creative Works

In many primate species, opponents may affiliate soon after an agonistic encounter. This study examined how the individual characteris- tics of opponents affected post-conflict interactions in a captive group of Japan- ese macaques (Macaca fuscata). Reconciliation and selective attraction of opponents was shown to occur following agonistic encounters. The factor most strongly influencing post-conflict interactions was age. Juveniles were significantly more likely than adults to initiate reconciliation. Sex, kinship, and dominance status were not associated with contact initiation. Because juveniles were the most likely victims of aggression, juvenile reconciliatory contact initiative may reduce the threat of further aggression.

The Potential For Ontogenetic Vertical Migration By Larvae Of Bathyal Echinoderms, William Jaeckle, Craig M. Young, Michael G. Devin, Suki U.K. Ekaratne, Sophie B. George

Scholarship

Planktotrophy is a relatively common developmental mode among bathyal and abyssal echinoderms, but the sources of food used by deep-sea planktotrophic larvae remain generally unknown. Very few deep-sea echinoderm larvae have been collected in plankton samples, so we do not know whether larvae migrate to the euphotic zone to feed or if they rely on bacteria or detritus at greater depths. We approached this question indirectly by investigating whether larvae of bathyal echinoids can tolerate the temperatures they would encounter in the euphotic zone and whether they possess sufficient energy stores to migrate to the euphotic zone without feeding. Twenty-four …

Book Review Of "The Battle Of Beginnings: Why Neither Side Is Winning The Creation-Evolution Debate" By Del Ratzsch, Carl J. Bajema

Other Scholarly Publications

The book is interesting for two reasons. First, the author presents numerous brief negative critiques of many of the arguments that creationists and scientists have employed in debates. Second, this book is an example of the recent strategy by Phillip Johnson and others to redirect the creation-evolution debate to such religious issues as intelligent design. This review will concentrate on Ratszch's discussion of (1) the imperfect nature of science, (2) design arguments and (3) who is entitled to be called a "creationist."

Captive Environmental Influences On Behavior In Zoo Drills And Mandrills (Mandrillus), A Threatened Genus Of Primate, Erik Terdal

Dissertations and Theses

Drills (Mandrillus leucophaeus) are an endangered species of African monkey (Cercopithecidae), and their sole congener the mandrill (Mandrillus sphinx) is vulnerable to extinction. Both species are threatened in the wild by deforestation and hunting.

Drills have a poor record of captive reproduction. Many individuals appear to have behavioral deficiencies which interfere with reproduction. Thus, the zoo population of drills does not serve as a “hedge” against the species’ total extinction: drills are endangered in captivity as well as in the wild. Mandrills, by contrast, reproduce well in captivity. Information on the behavior of mandrills in captivity may help zoo managers …

Communities Of Cloacal Bacteria In Tree Swallow Families, Michael P. Lombardo, Patrick A. Thorpe, R. Cichewicz, M. Henshaw, C. Millard, C. Steen, T. K. Zeller

Peer Reviewed Publications

Our aim in this study was to survey the communities of bacteria found in the cloacae of adult and nestling Tree Swallows (Tachycineta bicolor), determine if there were familial patterns of prevalence, and determine if there were relationships between bacteria loads and nestling size when 12 days old and fledging success.

A Pcr Assay For The Detection Of Wuchereria Bancrofti In Blood, Min Zhong, James Mccarthy, Lou Ann Bierwert, Michelle Lizotte-Waniewski, Suzanne Chanteau, Thomas Nutman, Eric A. Ottesen, Steven A. Williams

Biological Sciences: Faculty Publications

To identify Wuchereria bancrofti DNA sequences that could be used as the basis for a simple and rapid parasite detection assay, a genomic library of W. bancrofti was constructed and screened for highly repeated DNA. The repeat found with the highest copy number was 195 basepairs (bps) long, 77% AT, and 300 copies per haploid genome. This sequence was designated the Ssp I repeat because it has a unique recognition site for that restriction endonuclease in all or most of the repeat copies. The Ssp I repeat DNA family is dispersed, genus-specific, and exists in all of the different geographic …

The Effects Of Thyroid Hormone On Myosin Heavy Chain A-7 Production In Xenopus Laevis Tail And Leg Muscles, Andrew R. Noble

Honors Theses

The present research examines the expression of myosin heavy chain genes in muscle fibers in Xenopus laevis. Characteristically different muscle fibers are expressed before and after metamorphosis. The embryonic myosin proteins that are synthesized during the tadpole stages are replaced with adult myosin heavy chains at metamorphosis. At least one of the adult myosin genes, called A-7, is expressed only in the adult animals, not in tadpoles. The controlling factor or trigger for the expression of this adult gene still remains unknown. There are a number of possible explanations for the A-7 regulation at metamorphosis, including changes in particular hormone …

Genetic Variability And Gene Flow In Metepeira Ventura (Araneae, Araneidae), Martina G. Ramirez

Biology Faculty Works

Levels of genetic variability and gene flow among three populations of Metepeira ventura o n Santa Catalina Island, California, were evaluated based on variation at 10 gene loci . Mean heterozygosity (observed) per population was 10 .4% and mean polymorphism was 36 .7%, consistent with levels of variabilit y in other arthropods. Values of FST for the five polymorphic loci (mean FST = 0 .009) suggest that gene flow prevents the genetic differentiation of these populations . The average number of migrants per generation (Nm) among these populations is estimated to be 28 .6 . The lack of inter-population genetic …

Leptodactylus Silvanimbus (Amphibia: Anura: Leptodactylidae): Natural History Notes, Advertisement Call, And Relationships, W. Ronald Heyer, Rafael O. De Sá, James R. Mccranie, Larry David Wilson

Biology Faculty Publications

In July 1995, we visited two of the three previously known localities for Leprodactylus silvanimbus in Departamento de Ocotepeque, Honduras. We found a new breeding site for the species, recorded its advertisement call, and collected and observed its adults and larvae. The purpose of this paper is to present the results of these new findings and to comment on the relationships L. silvanimbus.

Características De La Osificación Craneal En Phyllomedusa Boliviana (Anura: Hylidae), Rafael O. De Sá, E. O. Lavilla

Biology Faculty Publications

RESUMEN. Dentro de la familia Hylidae disponemos de información sobre las secuencias de osificación craneal para menos del 4.0% de sus miembros. El presente trabajo describe la secuencia de osificación craneal de Phyllomedusa boliviana. El patrón general de osificación es similar a lo reportado para otras especies de la familia, pero existen algunas diferencias importantes tales como la osificación tardía de los próoticos y la osificación premetamórfica de neopalatino, vómer, cuadrado-yugal, pterigoides y columela. El lento desarrollo ulterior de la osificación resulta en juveniles con cráneos extensamente cartilaginosos.

ABSTRACT. Characteristics of the cranial ossification in Phyllomedusa …

Hyla Multifasciata, Rafael O. De Sá

Biology Faculty Publications

Hyla multifasciata is a medium to large species in the albopunctata species group, adults ranging in size from 42 - 61 mm SVL (mean male SVL = 48 mm, mean female SVL = 53 mm). The head is longer than wide. The snout is rounded in dorsal view, whereas in lateral view it is rounded and protrudes slightly beyond the mandible. The canthus rostralis is rounded and distinct, the loreal region is concave, and the internarial region is convex. The eyes are large, approximately twice the diameter of the tympanum. The tympanum is separated from the eye by a distance …

Afferent And Efferent Connections Of The Octavolateral Cerebellum In The Channel Catfish, Ictalurus Punctatus, Richard C. Rayborn

Master's Theses

No abstract provided.

The Distribution Of Gamma-Aminobutyric Acid (Gaba) And Glutamate In The Electrosensory System Of The Channel Catfish, Ictalurus Punctatus, Christian M. Fay

Master's Theses

No abstract provided.

Fate Of Biological Control Introductions: Monitoring An Australian Fungal Pathogen Of Grasshoppers In North America, Michael J. Bidochka, Scott R. A. Walsh, Mark E. Ramos, Raymond J. St. Leger, Julie Cl Silver, Donald W. Roberts

Biology Faculty Publications

In North America there are two generally recognized pathotypes (pathotypes 1 and 2) of the fungus Entomophaga grylli which show host-preferential infection of grasshopper subfamilies. Pathotype 3, discovered in Austra- lia,hasabroadergrasshopperhostrangeandwasconsidered to be a good biocontrol agent. Between 1989 and 1991 patho- type3wasintroducedattwofieldsitesinNorthDakota.Since resting spores are morphologically indistinguishable among pathotypes, we used pathotype-specific DNA probes to con- firm pathotype identification in E. grylli-infected grasshop- pers collected at the release sites in 1992, 1993, and 1994. In 1992, up to 23% of E. grylli-infected grasshoppers of the subfamilies Melanoplinae, Oedipodinae, and Gomphocerinae were infected by pathotype 3,with no infections >1 km …

Comparison Of The Effects Of Aerobic Dance To Water Aerobic Training On Maximal Oxygen Consumption, Terri L. Bedford, Tamara A. Dusterwinkle, Darcy J. Hoppman

Masters Theses

Water aerobics is one mode of exercise that is gaining popularity. Water aerobics allows adults who participate in an exercise program to improve their cardiorespiratory fitness. The purpose of this study was to determine if water aerobic training is as effective as land dance aerobic training in improving cardiorespiratory fitness in sedentary healthy adults. Eighteen subjects participated in this study; nine in dance aerobics and nine in water aerobics. Before and after eight weeks of training, subjects performed a graded maximal exercise test on a Schwinn Air-Dyne while maximal oxygen uptake was measured via a Beckman Metabolic Cart. ANCOVA was …

Sculpins As Predators Of Lake Trout Eggs And Larvae: An Assessment Of Their Predatory Capabilities And Constraints, Heather C. Biga

Master's Theses

No abstract provided.