Digital Commons Network^™

The Use Of Antibiotic Resistance Profiles To Access The Source Of Fecal Contamination In The Rowan County, Kentucky Watershed, Logan Harrison Burns

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Logan Harrison Burns on December 15, 2005.

Response Of Stress Levels In Domestic Canines To Calming Pheromone, Kathleen Barrett

Honors Capstones

Capstone submitted as a graduation requirement for the BSU Honors Program.

Exposure To Methamphetamine And Phencyclidine During Development And Subsequent Behavioral Change, Takehiro Minamoto

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Takehiro Minamoto on December 2, 2005.

Select Listeria Monocytogenes Subtypes Commonly Found In Foods Carry Distinct Nonsense Mutations In Inla, Leading To Expression Of Truncated And Secreted Internalin A, And Are Associated With A Reduced Invasion Phenotype For Human Intestinal Epithelial Cells, K. K. Nightingale, K. Windham, K. E. Martin, M. Yeung, M. Wiedmann

Biological Sciences

The surface protein internalin A (InlA) contributes to the invasion of human intestinal epithelial cells by Listeria monocytogenes. Screening of L. monocytogenes strains isolated from human clinical cases (n=46), foods (n=118), and healthy animals (n=58) in the United States revealed mutations in inlA leading to premature stop codons (PMSCs) in L. monocytogenes ribotypes DUP-1052A and DUP-16635A (PMSC mutation type 1), DUP-1025A and DUP-1031A (PMSC mutation type 2), and DUP-1046B and DUP-1062A (PMSC mutation type 3). While all DUP-1046B, DUP-1062A, DUP-16635A, and DUP-1031A isolates (n=76) contained inlA PMSCs, ribotypes DUP-1052A and DUP-1025A ( …

[Accepted Article Manuscript Version (Postprint)] Identification And Functional Characterization Of Arabidopsis Peroxin4 And The Interacting Protein Peroxin22, Bethany Zolman, Melanie Monroe-Augustus, Illeana Silva, Bonnie Bartel

Biology Department Faculty Works

Peroxins are genetically defined as proteins necessary for peroxisome biogenesis. By screening for reduced response to indole-3-butyric acid, which is metabolized to active auxin in peroxisomes, we isolated an Arabidopsis thaliana peroxin4 (pex4) mutant. This mutant displays sucrose-dependent seedling development and reduced lateral root production, characteristics of plant peroxisome malfunction. We used yeast two-hybrid analysis to determine that PEX4, an apparent ubiquitinconjugating enzyme, interacts with a previously unidentified Arabidopsis protein, PEX22. A pex4 pex22 double mutant enhanced pex4 defects, confirming that PEX22 is a peroxin. Expression of both Arabidopsis genes together complemented yeast pex4 or pex22 mutant defects, whereas expression …

Aquatic Animal Health Subprogram: Technical Guidelines For The Translocation Of Live Aquatic Animals - With Reference To Barramundi Nodavirus, Brian Jones

Fisheries Research Reports

FRDC Project No. 2005/640

A workshop was conducted involving input from most jurisdictions. Technical issues including hazard identification and risk management associated with the translocation of live finfish were documented.

A series of recommendations, which resulted from the workshop process, will be progressed through the National Aquatic Animal Health Technical Working group and the Aquatic Animal Health Committee.

Sal-Site: Integrating New And Existing Ambystomatid Salamander Research And Informational Resources, Jeramiah J. Smith, Srikrishna Putta, John A. Walker, D. Kevin Kump, Amy K. Samuels, James R. Monaghan, David W. Weisrock, Chuck Staben, S. Randal Voss

Biology Faculty Publications

Salamanders of the genus Ambystoma are a unique model organism system because they enable natural history and biomedical research in the laboratory or field. We developed Sal-Site to integrate new and existing ambystomatid salamander research resources in support of this model system. Sal-Site hosts six important resources: 1) Salamander Genome Project: an information-based web-site describing progress in genome resource development, 2) Ambystoma EST Database: a database of manually edited and analyzed contigs assembled from ESTs that were collected from A. tigrinum tigrinum and A. mexicanum, 3) Ambystoma Gene Collection: a database containing full-length protein-coding sequences, 4) Ambystoma Map and Marker …

Smooth Muscle Adherens Junctions Associated Proteins Are Stable At The Cell Periphery During Relaxation And Activation, Thomas J. Eddinger, Jessen D. Schiebout, Darl R. Swartz

Biological Sciences Faculty Research and Publications

This study was performed to determine the stability of the adherens junction (AJ)-associated proteins at the smooth muscle cell (SMC) plasma membrane during relaxing and activating conditions. Dog stomach, ileum, colon, and trachea tissues were stored in Ca²⁺-free PSS or regular PSS or were activated in 10 μM carbachol in PSS before rapid freezing. The tissues were subsequently sectioned and immunoreacted using antibodies for vinculin, talin, fibronectin, and caveolin to determine their cellular distribution in these tissues under these conditions. In all four tissues and under all three conditions, the distribution of these four proteins remained localized to …

Disentangling Above‐ And Below‐Ground Competition Between Lianas And Trees In A Tropical Forest, Stefan A. Schnitzer, Mirjam E. Kuzee, Frans Bongers

Biological Sciences Faculty Research and Publications

1. Light is thought to be the most limiting resource in tropical forests, and thus above‐ground competition is commonly accepted as the mechanism that structures these communities. In many tropical forests, trees compete not only with other trees, but also with lianas, which compete aggressively for below‐ground resources and thus may limit tree growth and regeneration.
2. Using a replicated experiment, we tested the relative strengths of above‐ and below‐ground competition from lianas on tree saplings in a disturbed forest in Côte d’Ivoire with a heterogeneous canopy and relatively high light penetration. We planted seedlings of three tree species and subjected them …

Avian Hosts For West Nile Virus In St. Tammany Parish, Louisiana, 2002, Nicholas Komar, Nicholas A. Panella, Stanley A. Langevin, Aaron C. Brault, Manuel Amador, Eric Edwards, Jennifer C. Owen

Faculty Publications

West Nile virus (WNV) infections in free-ranging birds were studied in Slidell, St. Tammany Parish, Louisiana, after a human encephalitis outbreak peaked there in July 2002. Seroprevalence in resident, free-ranging wild birds in one suburban site was 25% and 24% in August and October, respectively, indicating that most transmission had ceased by early August. Mortality rates, seroprevalence rates, host competence, and crude population estimates were used in mathematical models to predict actual infection rates, population impacts, and importance as amplifying hosts for several common passerine birds. Northern cardinal (Cardinalis cardinalis) and house sparrow (Passer domesticus) were the principal amplifying hosts, …

Applications Of Phylogenetics To Issues In Freshwater Crayfish Biology, Keith A. Crandall

Faculty Publications

Freshwater crayfish have served as model organisms for over 125 years in scientific research, from areas such as neurobiology and vision research to conservation biology and evolution. Recently, evolutionary histories in the form of phylogenies have served as a critical foundation for testing hypotheses in such diverse research areas as well. In this article, I review the amazing diversity of freshwater crayfish, especially in a phylogenetic context and explore how these evolutionary histories have informed crayfish biology and can be used powerfully in the future to guide research in a diversity of areas. Throughout the article, I draw on examples …

Hybridization Of Lythrurus Fasciolaris (Cope) And Lythrurus Umbratilis (Girard) (Cypriniformes: Cyprinidae) In The Ohio River Basin, Robert L. Hopkins Ii

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Robert L. Hopkins, II on November 14, 2005.

The Depressive Effect Of PI On The Force-Pca Relationship In Skinned Single Muscle Fibers Is Temperature Dependent, Edward Patrick Debold, Janell Romatowski, Robert H. Fitts

Biological Sciences Faculty Research and Publications

Increases in P_i combined with decreases in myoplasmic Ca2+ are believed to cause a significant portion of the decrease in muscular force during fatigue. To investigate this further, we determined the effect of 30 mM P_ion the force-Ca²⁺ relationship of chemically skinned single muscle fibers at near-physiological temperature (30°C). Fibers isolated from rat soleus (slow) and gastrocnemius (fast) muscle were subjected to a series of solutions with an increasing free Ca²⁺ concentration in the presence and absence of 30 mM P_iat both low (15°C) and high (30°C) temperature. In slow fibers, 30 mM …

Ego-1, A Putative Rna-Dependent Rna Polymerase, Is Required For Heterochromatin Assembly On Unpaired Dna During C. Elegans Meiosis, Eleanor M. Maine, Jessica Hauth, Thomas Ratliff, Valarie E. Vought, Xingyu She, William G. Kelly

Biology - All Scholarship

During meiosis in C. elegans, unpaired chromosomes and chromosomal regions accumulate high levels of histone H3 lysine 9 dimethylation (H3K9me2), a modification associated with facultative heterochromatin assembly and the resulting transcriptional silencing [1, 2]. Meiotic silencing of unpaired DNA may be a widely conserved genome defense mechanism [3–5]. The mechanisms of meiotic silencing remain unclear, although both transcriptional and posttranscriptional processes are implicated [3–5]. Cellular RNA-dependent RNA polymerases (RdRPs) function in development and RNA-mediated silencing in many species [3, 6, 7] and in heterochromatin assembly in S. pombe [3, 8]. There are four C. elegans RdRPs, including two with known …

Plumage Convergence In Picoides Woodpeckers Based On A Molecular Phylogeny, With Emphasis On Convergence In Downy And Hairy Woodpeckers, Amy C. Weibel, William S. Moore

Biological Sciences Faculty Research Publications

Adult and juvenile plumage characters were traced onto a well-resolved molecular based phylogeny for Picoides woodpeckers, and a simple phylogenetic test of homology, parallelism, and convergence of plumage characters was performed. Reconstruction of ancestral character states revealed multiple events of independent evolution of derived character states in most characters studied, and a concentrated changes test revealed that some plumage characters evolved in association with habitat type. For example, there was a statistically significant association between loss of dorsal barring and use of densely vegetated habitats among Picoides species. Two analyses indicated that convergence, as opposed to parallel evolution or shared …

Flexible Social Structure Of A Desert Rodent, Rhombomys Opimus: Philopatry, Kinship, And Ecological Constraints, Jan Randall, Konstantin Rogovin, Patricia Parker, John Eimes

Biology Department Faculty Works

We tested hypotheses based on philopatry, kinship, and ecological constraints to explain sociality in a semifossorial desert rodent, the great gerbil, Rhombomys opimus. Data were collected in the field in Uzbekistan in the spring and fall of 1996 and 1998–2004. Population densities fluctuated dramatically with high turnover in both males and females to reveal that dispersal and social structure were density dependent. Fewer gerbils dispersed at higher densities and members of family groups dispersed together. A majority of females lived in groups at high densities, but as population densities declined, proportionally more females were solitary. DNA analysis revealed that group-living …

Bateman Gradients In Field And Laboratory Studies: A Cautionary Tale, Patricia Parker, Zuleyma Tang-Martinez

Biology Department Faculty Works

Since tools of molecular genetics became readily available, our understanding of bird mating systems has undergone a revolution. The majority of passerine species investigated are socially monogamous, but have been shown to be genetically polygamous. Data sets from natural populations of juncos suggest that multiple mating by females results in a sexual selection gradient as steep for females as for males (a result that does not support Bateman's predictions). However, in males, fitness is enhanced directly through fertilization success with multiple matings; in females fitness benefits may be enhanced immediately through direct access to food, protection against predators, or other …

Modulation Of Tyramine Signaling By Osmolality In An Insect Secretory Epithelium, Edward M. Blumenthal

Biological Sciences Faculty Research and Publications

The control of water balance in multicellular organisms depends on absorptive and secretory processes across epithelia. This study concerns the effects of osmolality on the function of the Malpighian tubules (MTs), a major component of the insect excretory system. Previous work has shown that the biogenic amine tyramine increases transepithelial chloride conductance and urine secretion in Drosophila MTs. This study demonstrates that the response of MTs to tyramine, as measured by the depolarization of the transepithelial potential (TEP), is modulated by the osmolality of the surrounding medium. An increase in osmolality caused decreased tyramine sensitivity, whereas a decrease in osmolality …

Rapid Senescence In Pacific Salmon, Yolanda E. Morbey, Chad Brassil, Andrew P. Hendry

School of Biological Sciences: Faculty Publications

Any useful evolutionary theory of senescence must be able to explain variation within and among natural populations and species. This requires a careful characterization of age-specific mortality rates in nature as well as the intrinsic and extrinsic factors that influence these rates. We perform this task for two populations of semelparous Pacific salmon. During the breeding season, estimated daily mortality rates increased from 0 to 0.2–0.5 (depending on the year) over the course of several weeks. Early-arriving individuals had a later onset and/or a lower rate of senescence in each breeding season, consistent with adaptive expectations based on temporal variation …

Contributions Of Heterosis And Epistasis To Hybrid Fitness, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Early-generation hybrid fitness is difficult to interpret because heterosis can obscure the effects of hybrid breakdown. We used controlled reciprocal crosses and common garden experiments to distinguish between effects of heterosis and nuclear and cytonuclear epistasis among morphotypes and advanced-generation hybrid derivative populations in the Piriqueta caroliniana (Turneraceae) plant complex. Seed germination, growth, and sexual reproduction of first-generation hybrids, inbred parental lines, and outbred parental lines were compared under field conditions. Average vegetative performance was greater for hybrids than for inbred lines, and firstseason growth was similar for hybrids and outbred parental lines. Hybrid survival surpassed that of inbred lines …

Carotenoid Availability Affects The Development Of A Colour-Based Mate Preference And The Sensory Bias To Which It Is Genetically Linked, Gregory F. Grether, Gita R. Kolluru, F. Helen Rodd, Jennifer De La Cerda, Kaori Shimazaki

Biological Sciences

Regardless of their origins, mate preferences should, in theory, be shaped by their benefits in a mating context. Here we show that the female preference for carotenoid colouration in guppies (Poecilia reticulata) exhibits a phenotypically plastic response to carotenoid availability, confirming a key prediction of sexual selection theory. Earlier work indicated that this mate preference is genetically linked to, and may be derived from, a sensory bias that occurs in both sexes: attraction to orange objects. The original function of this sensory bias is unknown, but it may help guppies find orange-coloured fruits in the rainforest streams of …

Regulatory Functions Of Phospholipase D And Phosphatidic Acid In Plant Growth, Development, And Stress Responses, Xuemin Wang

Biology Department Faculty Works

No abstract provided.

Evolution Of Sexual Dimorphism And Male Dimorphism In The Expression Of Beetle Horns: Phylogenetic Evidence For Modularity, Evolutionary Lability, And Constraint, Douglas J. Emlen, John Hunt, Leigh W. Simmons

Biological Sciences Faculty Publications

Beetle horns are enlarged outgrowths of the head or thorax that are used as weapons in contests over access to mates. Horn development is typically confined to males (sexual dimorphism) and often only to the largest males (male dimorphism). Both types of dimorphism result from endocrine threshold mechanisms that coordinate cell proliferation near the end of the larval period. Here, we map the presence/absence of each type of dimorphism onto a recent phylogeny for the genus Onthophagits (Coleoptera: Scarabaeidae) to explore how horn development has changed over time. Our results provide empirical support for several recent predictions regarding the evolutionary …

Fisheries Occasional Publication No. 32 - Allocation Of The Western Australian Abalone Resource Between User Groups, Department Of Fisheries Western Australia

Fisheries Occasional Publications

In this submission to the Integrated Fisheries Allocation Advisory Committee (IFAAC), the Department will outline what it believes are the most practical and cost effective ways of managing a system for allocating the abalone resource between the commercial, customary and recreational sectors.

In preparing this submission, it has been noted that because of the limited data on recreational catch outside of the metropolitan area, the Sustainable Harvest Limit (SHL) set for the fishery relates only to the area that is equivalent to Area 7 of the commercial fishery. Further, it is acknowledged that the Minister has approved that proportional catch …

A Phylogenetic Analysis Of Vanzolinius Heyer, 1974 (Amphibia, Anura, Leptodactylidae): Taxonomic And Life History Implications, Rafael O. De Sá, W. Ronald Heyer, Arley Camargo

Biology Faculty Publications

The validity of the monotypic leptodactylid frog genus Vanzolinius Heyer, 1974 has been questioned recently. We explore the relationship of Vanzolinius discodactylus within the cluster of closely related genera Adenomera, Leptodacylus, and Lithodytes with both morphological and molecular data sets. Morphological and combined morphological and molecular data were analyzed using maximum parsimony; molecular data sets were analyzed with maximum likelihood methods. The resultant relationships are unambiguous in Vanzolinius being imbedded within Leptodactylus. In order to maintain Leptodactylus as a monophyletic genus, Vanzolinius is placed in the synonymy of Leptodactylus Fitzinger, 1826. The implications of relationships analyzed in …

Changes In Bird Abundance After Wildfire: Importance Of Fire Severity And Time Since Fire, Kristina M. Smucker, Richard L. Hutto, Brian M. Steele

Biological Sciences Faculty Publications

Fire can cause profound changes in the composition and abundance of plant and animal species, but logistics, unpredictability of weather, and inherent danger make it nearly impossible to study high-severity fire effects experimentally. We took advantage of a unique opportunity to use a before–after/control–impact (BACI) approach to analyze changes in bird assemblages after the severe fires of 2000 in the Bitterroot Valley, Montana. Observers surveyed birds using 10-minute point counts and collected vegetation data from 13 burned and 13 unburned transects for five years before fire and three years after fire. We compared changes in vegetation variables and relative bird …

Redescripción De La Morfología Larval Externa De Dos Especies Del Grupo De Leptodactylus Fuscus (Anura, Leptodactylidae), José A. Langone, Rafael O. De Sá

Biology Faculty Publications

Re-description of the larval external morphology of two species of the Leptodactylus fuscus group (Anura, Leptodactylidae). The larvae of Leptodactylus gracilis (D’Orbigny y Bibron, 1840) and L. mystacinus Bumeister, 1861 are re-described and compared with previous descriptions noting intraspecific variation in oral disc characteristics (arrangement of papillae and rows of cornified teeth). The external morphological analysis of larvae of the Leptodactylus fuscus group suggest that the lack of comparable descriptions among species, as well as the lack of analyses of their intraspecific variation, limits the use of larval characteristics for diagnostic purposes.

Online Dictionary Of Invertebrate Zoology: F, Mary Ann Basinger Maggenti, Armand R. Maggenti, Scott L. Gardner

Armand R. Maggenti Online Dictionary of Invertebrate Zoology

face facet facette facetted facial facialium facies facio-orbital factor facultative faeces Fahrenholz's falcate falces falciform falciger falcis falculate falsadentes false falx famulus fan fang farctate farinaceous fascia fasciate fascicle fasciculus fasciole fascio-maculata fascio-punctate fastening fastigiate fastigium fat fate fauces fauna faunal faveolate feces fecula fecundate fecundity feedback feeder feeding feeler feet felt female femoro-alary femorogenu femorotibial femur fenestra fenestrate fenestration fenestrella fenestrule fente feral ferment fermentation ferreous ferruginous ferrule fertile fertility fertilization fertilizin festivus festoon festoons fetid fiber fibril fibrillar fibrin fibrinogen fibrocyte fibroin fibroplasm fibrous fibula fide filament filamentary filaria filariform filate filator file filial filiation filibranch filicornia filiform …

Online Dictionary Of Invertebrate Zoology: B, Mary Ann Basinger Maggenti, Armand R. Maggenti, Scott L. Gardner

Armand R. Maggenti Online Dictionary of Invertebrate Zoology

baccate bacillary bacilliform back-cross back-mutation bacteremia bacteriophagous baculiform baculite baenomere baenopoda baenosome Baer's bailer balanced balancers balanoid balantin balantine Balbiani Balbiani's bald Baldwin ballonets ballooning band barb barbate, barbatus barbula bark baroceptor, baroreceptor barotropism barrier basad basal basalar basalia basalis base basement bases basicarnal basicerite basiconic basicosta basicostal basicoxite basifemoral basifemur basilaire basilar basilateral basilemma basimandibula basimaxilla basimetrical basinym basioccludent basiophthalmite basiperiphallus basipharynx basipod basipodite basiproboscis basipulvilli basirostral basis basiscopic basiscutal basisternum basistylus basitarsal basitarsus basitergal basitibial basitrich basitrichous basivalvula basket basopinacocytes batatiform Batesian bathmis bathyal bathylimnetic bathymetric bathypelagic battery batumen bave B-chromosome, bdelloid bead beaded beak beaked bean beard …

Online Dictionary Of Invertebrate Zoology: A, Mary Ann Basinger Maggenti, Armand R. Maggenti, Scott L. Gardner

Armand R. Maggenti Online Dictionary of Invertebrate Zoology

abactinal abambulacral A-band abapertural abapical abaxial abbreviate abcauline abdomen abduct aberrant abient abiocoen abiogenesis abiology abiotic abjugal ablation aboral aboriginal abortion abranchiate abreptor abscission absorption abterminal abullate abyssal abyssobenthos abyssopelagic acanthaceous acanthella acanthocephalans acanthocyst acanthodion acanthodrilin acanthoparia acanthophore acanthophorites acanthopod acanthosphenote acanthostegous acanthostyle acanthozooid acariasis Acarina acarinarium acarocecidium acarology acarophily acaryote acaudal acclimation acclivous accretion acelomate acentric Acephala acephalocyst acephalous acerata acervate acescence acetabulum achaetous achlamydate achroacyte achroglobin achroic achromasia achromatic A-chromosome achroous acia acicle aciculiform acidic acidobiontic acidophilic acidophobic acidotheca aciduric acies aciform acinacicate acinaciform acinarious aciniform acinose aclid acline aclitellate acme Acoelomata acoelous acone acontioids acraein Acraspeda acrembolic …