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Social Grouping Behaviors Of Captive Female Hippopotamus Amphibius, Tracy Blowers

Electronic Theses and Dissertations

Levels of sociality vary depending on the costs and benefits associated with grouping behavior. Grouping species form either ephemeral aggregations due to resource availability, or structured groups based on familiarity of individuals. Because there are different costs and benefits associated with different types of groups, it is important to understand more about group structure before making predictions about specific behaviors. Female Hippopotamus amphibius are known to aggregate in the wild but the true nature of their grouping behavior is still not understood. My objective was to determine if captive female hippos form either ephemeral aggregations or social groups. Behavioral data, …

Analysis Of Wnt Signaling During Caenorhabditis Elegans Postembryonic Development, Samantha Van Hoffelen, Michael A. Herman

School of Biological Sciences: Faculty Publications

Wnts play a central role in the development of many cells and tissue types in all species studied to date. Like many other extracellular signaling pathways, secreted Wnt proteins are involved in many different processes; in C. elegans these include cell proliferation, differentiation, cell migration, control of cell polarity, axon outgrowth, and control of the stem cell niche. Perturbations in Wnt signaling are also key factors in cancer formation, and therefore of interest to oncobiologists. Wnts are secreted glycoproteins, which bind to Frizzled transmembrane receptors and signal either through, or independently of β-catenin. Both β-catenin-dependent (Wnt/β-catenin) and -independent pathways function …

"A Global Assessment Of Freshwater Crab Diversity" , Darren Cj Yeo, Peter Kl Ng, Neil Cumberlidge, Celio Magalhães, Savel R. Daniels, Martha R. Campos

Journal Articles

No abstract provided.

"A Revision Of The Higher Taxonomy Of The Afrotropical Freshwater Crabs With A Discussion Of Their Biogeography" , Neil Cumberlidge, Richard M. Von Sternberg, Savel R. Daniels

Journal Articles

No abstract provided.

Chihuahuan Desertkangaroo Rats: Nonlinear Effects Of Population Dynamics, Competition, And Rainfall, M. Lima, S.K. Morgan Ernest, J. H. Brown, A. Belgrano, N. C. Stenseth

Biology Faculty Publications

Using long-term data on two kangaroo rats in the Chihuahuan Desert of North America, we fitted logistic models including the exogenous effects of seasonal rainfall patterns. Our aim was to test the effects of intraspecific interactions and seasonal rainfall in explaining and predicting the numerical fluctuations of these two kangaroo rats. We found that logistic models fit both data sets quite well; Dipodomys merriami showed lower maximum per capita growth rates than Dipodomys ordii, and in both cases logistic models were nonlinear. Summer rainfall appears to be the most important exogenous effect for both rodent populations; models including this variable …

On Estimating The Exponent Of Power-Law Frequency Distributions, Ethan P. White, B. J. Enquist, J. L. Green

Biology Faculty Publications

Power-law frequency distributions characterize a wide array of natural phenomena. In ecology, biology, and many physical and social sciences, the exponents of these power-laws are estimated to draw inference about the processes underlying the phenomenon, to test theoretical models, and to scale up from local observations to global patterns. Therefore, it is essential that these exponents be estimated accurately. Unfortunately, the binning-based methods traditionally utilized in ecology and other disciplines perform quite poorly. Here we discuss more sophisticated methods for fitting these exponents based on cumulative distribution functions and maximum likelihood estimation. We illustrate their superior performance at estimating known …

On The Relationship Between Mass And Diameter Distributions In Tree Communities, J. C. Stegen, Ethan P. White

Biology Faculty Publications

It has been suggested that frequency distributions of individual tree masses in natural stands are characterized by power-law distributions with exponents near -3/4, and that therefore tree communities exhibit energetic equivalence among size classes. Because the mass of trees is not measured directly, but estimated from diameter, this supposition is based on the fact that the observed distribution of tree diameters is approximately characterized by a power-law with an exponent -2. Here we show that diameter distributions of this form are not equivalent to mass distributions with exponents of -3/4, but actually to mass distributions with exponents of -11/8. We …

Zero-Sum, The Niche,And Metacommunities: Long-Term Dynamics Of Community Assembly, S.K. Morgan Ernest, J. H. Brown, K. M. Thibault, Ethan P. White, J. R. Goheen

Biology Faculty Publications

Recent models of community assembly, structure, and dynamics have incorporated, to varying degrees, three mechanistic processes: resource limitation and interspecific competition, niche requirements of species, and exchanges between a local community and a regional species pool. Synthesizing 30 years of data from an intensively studied desert rodent community, we show that all of these processes, separately and in combination, have influenced the structural organization of this community and affected its dynamical response to both natural environmental changes and experimental perturbations. In addition, our analyses suggest that zero-sum constraints, niche differences, and metacommunity processes are inextricably linked in the ways that …

The Effect Of Road Crossing On Fish Movements In Small Etowah Basin Streams, Paul D. Benton, William E. Ensign, Byron J. Freeman

Faculty Articles

Increased road construction associated with urbanization may result in fragmentation and loss offish populations in streams. In this study, we documented frequency of movement of fishes through three separate types of road-crossings (clear-span bridges, box culverts, and tube culverts) in six small streams using mark-recapture sampling. Upstream movement between areas separated by either box or tube culverts was lower than upstream movement between similar areas not separated by a road crossing. Downstream movement between areas separated by box culverts was also lower than downstream movement between areas without obstructions. Upstream and downstream movement between areas separated by clear-span bridges was …

Conservation Of Selection On Matk In Ferns Following An Ancient Loss Its Flanking Intron, A. M. Duffy, S. A. Kelchner, Paul G. Wolf

Biology Faculty Publications

The chloroplast gene trnK and its associated group II intron appear to be absent in a large and ancient clade that includes nearly 90% of fern species. However, the maturase protein encoded within the intron (matK) is still present and located on the boundary of a large-scale inversion. We surveyed the chloroplast genome sequence of clade-member Adiantum capillus-veneris for evidence of a still present but fragmented trnK intron. Lack of signature structural domains and sequence motifs in the genome indicate loss of the trnK intron through degradation in an ancestor of the clade. In plants, matK preferentially catalyzes splicing of …

Wind, Waves, And Wing Loading: Their Relative Importance To The At-Sea Distribution And Movements Of North And Central Pacific Albatrosses, R M. Suryan, D J. Anderson, Scott A. Shaffer, D D. Roby, Y Tremblay, D P. Costa, F Sato, K Ozaki, G Balogh

Faculty Publications, Biological Sciences

Among the varied adaptations for avian flight, the morphological traits allowing large-bodied albatrosses to capitalize on wind and wave energy for efficient long-distance flight are unparalleled. Consequently, the biogeographic distribution of most albatrosses is limited to the windiest oceanic regions on earth; however, exceptions exist. Species breeding in the North and Central Pacific Ocean (Phoebastria spp.) inhabit regions of lower wind speed and wave height than southern hemisphere genera, and have large intrageneric variation in body size and aerodynamic performance. Here, we test the hypothesis that regional wind and wave regimes explain observed differences in Phoebastria albatross morphology and we …

Perspectives In Ornithology: Application Of Tracking And Data-Logging Technology In Research And Conservation Of Seabirds, A E. Burger, Scott A. Shaffer

Faculty Publications, Biological Sciences

No abstract provided.

Diet Of Western Slimy Salamander, Plethodon Albagula (Caudata: Plethodontidae), From Two Mountain Ranges In Arkansas, Joseph Milanovich, Stanley E. Trauth, Tanja Mckay

Biology: Faculty Publications and Other Works

We identified stomach contents of 80 Plethodon albagula (Western Slimy Salamander) from two mountain ranges in Arkansas (Ozark and Ouachita) to examine if regional differences in diet occur. Museum specimens from 1985 to 2005 were used from locations throughout each mountain range. Although a wide variety of prey were found in stomachs, Hymenoptera: Formicidae and Coleoptera: Carabidae were found to be the most important food items in the diet of P. albagula. Ants and beetles constituted 85% abundance of their total diet (79.6% Ozark and 90.1% Ouachita) and 52.2% of their total biomass (42.6% Ozark and 64.1% Ouachita). Seventy-eight and …

Selective Gene Expression By Postnatal Electroporation During Olfactory Interneuron Nurogenesis, Alexander T. Chesler, Claire E. Le Pichon, Jessica H. Brann, Ricardo C. Araneda

Biology: Faculty Publications and Other Works

Neurogenesis persists in the olfactory system throughout life. The mechanisms of how new neurons are generated, how they integrate into circuits, and their role in coding remain mysteries. Here we report a technique that will greatly facilitate research into these questions. We found that electroporation can be used to robustly and selectively label progenitors in the Subventicular Zone. The approach was performed postnatally, without surgery, and with near 100% success rates. Labeling was found in all classes of interneurons in the olfactory bulb, persisted to adulthood and had no adverse effects. The broad utility of electroporation was demonstrated by encoding …

Comparative Diet Of Three Sympatric Sceloporus In The Semiarid Zapotitlan Valley, Mexico, V. Hugo Serrano-Cardozo, J. A. Lemos-Espinal, Geoffrey R. Smith

Faculty Publications

No abstract provided.

Towards Cloning The Clk-3 Gene In Caenorhabditis Elegans, Suchita Umesh Desai

University of Kentucky Master's Theses

Mutations in the clk-1, clk-2, clk-3 and gro-1 genes in Caenorhabditis elegans show alterations in developmental and behavioral timing and lifespan, collectively termed the Clk phenotype. While the clk-1, clk-2, and gro-1 genes have been cloned, clk-3 gene has not been identified. Gene expression changes in clk-3 mutant worms were determined using microarray expression data. I examined genes in the region to which clk-3 gene maps, for strongly reduced expression in the clk-3 mutants and identified thirteen clk-3 candidate genes. RNAi feeding vectors for all these candidate genes were picked and cultured from the RNAi library. …

Functional Characterization Of Upd3 In Drosophila Development, Liqun Wang

University of Kentucky Doctoral Dissertations

The JAK/STAT pathway is a non-receptor tyrosine kinase signaling pathway that is well conserved and highly re-utilized in many mammalian and Drosophila developmental processes. Compared to dozens of ligands and receptors in mammalian JAK/STAT, Drosophila JAK/STAT pathway is simpler with one receptor and three ligands, Upd, Upd2 and Upd3, which have similar amino acid sequences. Previous literature shows that upd and upd2 exhibit the same dynamic striped expression pattern in embryos and have semi-redundant functions during embryogenesis. Do Upd and Upd3 also have redundant functions? To answer this question, the functions of Upd3 in Drosophila development were investigated in this …

The Influence Of Ca2+ Regulation In Synaptic Facilitation Of Motor Nerve Terminals In Crayfish And Drosophila As Well As In The Physiological Regulation Of Larval Drosophila Heart, Mohati Desai-Shah

University of Kentucky Doctoral Dissertations

Intracellular Ca²⁺ ions are highly regulated in animal cells for them to function normally. Since the tight regulation of [Ca²⁺]i is so ubiquitous among cells, it is not surprising that altered function in [Ca²⁺]i regulation is associated with a myriad of disease states in humans. This is particularly evident in pacing myocytes and nerve terminals related to synaptic transmission. A common thread through this dissertation is on the role of three regulators proteins that are common to many cell types. These are the plasmalemmal Na⁺/Ca²⁺ exchanger (NCX), the Ca²⁺-ATPase (PMCA) …

Rapid Dissemination Of Francisella Tularensis And The Effect Of Route Of Infection, S. S. Ojeda, Z. J. Wang, Chris A. Mares, T. A. Chang, Q. Li, E. G. Morris, P. A. Jerabek, J. M. Teale

Biology Faculty Publications (Archived)

Background. Francisella tularensis subsp. tularensis is classified as a Category A bioweapon that is capable of establishing a lethal infection in humans upon inhalation of very few organisms. However, the virulence mechanisms of this organism are not well characterized. Francisella tularensis subsp. novicida, which is an equally virulent subspecies in mice, was used in concert with a microPET scanner to better understand its temporal dissemination in vivo upon intranasal infection and how such dissemination compares with other routes of infection. Adult mice were inoculated intranasally with F. tularensis subsp. novicida radiolabeled with 64Cu and imaged by microPET at 0.25, 2 …

Polyomaviruses In Pediatric Renal Disease, Julie Moran

Masters

BK virus (BKV) and JC virus (JCV) are ubiquitous in the human population and frequently reactivate during periods of immune suppression. The emergence of polyomavirus-associated diseases among renal and hematopoietic stem cell transplant recipients has highlighted the lack of knowledge of the natural history of BKV and JCV in humans. In the pediatric population, in particular, immune suppressive therapy is commonly used as a treatment for primary renal diseases. To date, there has been limited investigation of polyomavirus reactivation in pediatric patients with renal transplants or with primary renal disease. Given the association of polyomavirus reactivation with the use of …

Role Of Microtubules In Budding Yeast Cytokinesis, Su Young Park

Masters Theses

"Cytokinesis is an important step to finish cell cycle events and separate into two daughter cells. This event is driven by actomyosin ring contraction. In budding yeast, the mitotic exit network (MEN) controls completion of ana/telophase by signaling events. Bub2 is a mitotic exit network regulator and binds to Tem1p which is an important component in MEN. Mad2 is a component of a separate spindle checkpoint. Mad2 dependent pathway prevents the metaphase to anaphase transition under spindle damage. Because yeast cells have cell walls, septum formation follows actin ring contraction. Here it was investigated how microtubule defects affect cytokinesis in …

Validation Of An Nsp-Based (Negative Selection Pattern) Gene Family Identification Strategy, Ronald L. Frank, Cyriac Kandoth, Fikret Erçal

Biological Sciences Faculty Research & Creative Works

Background: Gene family identification from ESTs can be a valuable resource for analysis of genome evolution but presents unique challenges in organisms for which the entire genome is not yet sequenced. We have developed a novel gene family identification method based on negative selection patterns (NSP) between family members to screen EST-generated contigs. This strategy was tested on five known gene families in Arabidopsis to see if individual paralogs could be identified with accuracy from EST data alone when compared to the actual gene sequences in this fully sequenced genome. Results: The NSP method uniquely identified family members in all …

An Expression Profile Of Cadherin-11 In Developing Chicken Embryos, Kacie Flaherty

Dissertations and Theses @ UNI

The properties of cadherin-11 adhesiveness allow it to play a dynamic role in development. Known primarily as a mesenchymal cadherin, cadherin-11 expression may be correlated with morphogenetic events in developing embryos. Although cadherin-11 expression has been studied in the mouse and Xenopus, its expression pattern in the important avian model has not been described. We investigated cadherin-11 's expression profile using a monoclonal antibody in fluorescence immunolocalization, assisted by microwave antigen retrieval; in Hamburger-Hamilton stages 13-22 chicken embryos (approx. 46-96 hrs). Cadherin-11 was expressed in mesenchymal tissue surrounding the eye, pharynx and aortic arches, auditory vesicles, lung buds and stomach, …

Series Activity: Watching Wildlife [Iowa Academy Of Science National Wildlife Audio Series], Iowa Academy Of Science

Open Educational Resources

Observing wildlife can be fun whether you are at one of Iowa’s National Wildlife Refuges, a local park or your own back yard. Make watching wildlife a hobby and you will begin to recognize habits and patterns in the actions of the wildlife around you. You might be able to predict when certain wildlife will come to an area to feed, the sounds a bird makes when attempting to protect its young or what will trigger a rabbit to freeze in place. You’ll also be practicing the skills scientists use to make their observations: comparing and contrasting, measuring, counting and …

Stressor- And Corticotropin Releasing Factor-Induced Reinstatement And Active Stress-Related Behavioral Responses Are Augmented Following Long-Access Cocaine Self-Administration By Rats, John R. Mantsch, David A. Baker, David M. Francis, Eric S. Katz, Michael A. Hoks, Joseph P. Serge

Biomedical Sciences Faculty Research and Publications

Rationale Stressful events during periods of drug abstinence likely contribute to relapse in cocaine-dependent individuals. Excessive cocaine use may increase susceptibility to stressor-induced relapse through alterations in brain corticotropin-releasing factor (CRF) responsiveness.

Objectives This study examined stressor- and CRF-induced cocaine seeking and other stress-related behaviors in rats with different histories of cocaine self-administration (SA).

Materials and methods Rats self-administered cocaine under short-access (ShA; 2 h daily) or long-access (LgA; 6 h daily) conditions for 14 days or were provided access to saline and were tested for reinstatement by a stressor (electric footshock), cocaine or an icv injection of CRF and …

Effect Of Thyroid Hormone Concentration On The Transcriptional Response Underlying Induced Metamorphosis In The Mexican Axolotl (Ambystoma), Robert B. Page, S. R. Voss, A. K. Samuels, J. J. Smith, S. Putta, C. K. Beachy

Biology Faculty Publications (Archived)

Background: Thyroid hormones (TH) induce gene expression programs that orchestrate amphibian metamorphosis. In contrast to anurans, many salamanders do not undergo metamorphosis in nature. However, they can be induced to undergo metamorphosis via exposure to thyroxine (T4). We induced metamorphosis in juvenile Mexican axolotls (Ambystoma mexicanum) using 5 and 50 nM T4, collected epidermal tissue from the head at four time points (Days 0, 2, 12, 28), and used microarray analysis to quantify mRNA abundances. Results: Individuals reared in the higher T4 concentration initiated morphological and transcriptional changes earlier and completed metamorphosis by Day 28. In contrast, initiation of metamorphosis …

Transcriptional Response Of Mexican Axolotls To Ambystoma Tigrinum Virus (Atv) Infection, J. D. Cotter, A. Storfer, Robert B. Page, C. K. Beachy, S. R. Voss

Biology Faculty Publications (Archived)

Background: Very little is known about the immunological responses of amphibians to pathogens that are causing global population declines. We used a custom microarray gene chip to characterize gene expression responses of axolotls (Ambystoma mexicanum) to an emerging viral pathogen, Ambystoma tigrinum virus (ATV). Result: At 0, 24, 72, and 144 hours post-infection, spleen and lung samples were removed for estimation of host mRNA abundance and viral load. A total of 158 up-regulated and 105 down-regulated genes were identified across all time points using statistical and fold level criteria. The presumptive functions of these genes suggest a robust innate immune …

Failure To Replicate Evidence Of Decapod Crustacean Nociception, Sakshi Puri, Zen Faulkes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Introduction Here, we attempt to replicate their experiments with two other species of decapod crustaceans that bracket P. elegans phylogenetically: white shrimp (Litopenaeus setiferus) and Louisiana red swamp crayfish (Procambarus clarkii). Litopenaeus setiferus have a similar ecology to prawns. Procambarus clarkii is widely used in the study of neurobiology, and would be an excellent candidate for further neurophysiological studies.

Discussion We found no behavioral evidence of nociception in either L. setiferus or P. clarkii. This outcome contrasts sharply with results that indicated P. elegans had nociception (Barr et al. 2007).

Additions To The Breeding Avifauna Of The Lower Rio Grande Valley Of Texas, Timothy Brush

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The breeding avifauna of the Lower Rio Grande Valley (LRGV) of Texas has experienced many changes during the 20th century, primarily because of declines in native habitats due to land being converted to agriculture and urban habitats. This paper summarizes changes in breeding avifauna from 2003–2007 in the area. Breeding has been confi rmed for Cooper’s Hawk (Accipiter cooperii), Eurasian Collared-Dove (Streptopelia decaocto), and Mangrove Yellow Warbler (Dendroica petechia oraria) for the fi rst time, and for Gray-crowned Yellowthroat (Geothlypis poliocephala) and Ferruginous Pygmy-Owl (Glaucidium brasilianum) after periods of apparent absence. Short-tailed Hawk (Buteo brachyurus) is a possible breeder, based …

Retinal Tissue Engineering Using Mouse Retinal Progenitor Cells And A Novel Biodegradable, Thin-Film Poly(E-Caprolactone) Nanowire Scaffold, Stephen Redenti, Sarah Tao, Jing Yang, Ping Gu, Henry Klassen, Sunita Saigal, Tejal Desai, Michael J. Young

Publications and Research

Retinal progenitor cells (RPCs) can be combined with nanostructured polymer scaffolds to generate compos- ite grafts in culture. One strategy for repair of diseased retinal tissue involves implantation of composite grafts of this type in the subretinal space. In the present study, mouse retinal progenitor cells (RPCs) were cultured on laminin- coated novel nanowire poly(e-caprolactone)(PCL) scaf- folds, and the survival, differentiation, and migration of these cells into the retina of C57bl/6 and rhodospsin −/−mouse retinal explants and transplant recipients were analyzed. RPCs were cultured on smooth PCL and both short (2.5μm) and long (27μ m) nanowire PCL scaffolds. Scaffolds with …