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Effect Of Redox-Cycling Agents On Nitric Oxide Synthase Activity In Vitro And Modulation Of Protein Expression In Vivo: No Nitric Oxide Synthase In Mitochondria, Priya Venkatakrishnan

Open Access Theses & Dissertations

This Dissertation describes studies conducted in three areas dealing with the interaction of redox-active compounds with the flavin- and heme- containing enzyme, neuronal nitric oxide synthase (NOS). They are as follows:

I. Studies were initiated using extracts from several varieties of tobacco to identify constituent(s) that could interact with nNOS. Results from [¹⁴C]-L-arginine to [¹⁴C]-L-citrulline conversion assays to which tobacco extracts were individually added, indicate that there are nNOS inhibitors present in tobacco and they are non-polar, lipophilic and non-volatile in nature. In addition, results from assays in which the tobacco-derived redox-active compound, 2,3,6- …

"A New Species Of Freshwater Crab From Western Kenya" , Neil Cumberlidge, Michael Dobson

Journal Articles

No abstract provided.

Out Of South America: Multiple Origins Of Non-Native Apple Snails In Asia, Kenneth Hayes

Department of Biology Faculty Publications

Apple snails (Ampullariidae: Pomacea) native to the New World have become agricultural and environmental pests widely in southern and eastern Asia since their introduction in about 1980. Although their impacts have been extensively documented, considerable confusion persists regarding their identities and geographical origins. Efforts to resolve the confusion have suffered from inadequate taxonomic and geographical sampling from both native and introduced ranges. Using phylogenetic and genealogical methods, we analysed 610–655 bp of cytochrome c oxidase subunit I DNA sequences from 783 apple snails from 164 Asian locations and 57 native South American locations. In Asia, we found four species of …

The Spinobulbar System In Lamprey, James T. Buchanan, James F. Einum

Biological Sciences Faculty Research and Publications

Locomotor networks in the spinal cord are controlled by descending systems which in turn receive feedback signals from ascending systems about the state of the locomotor networks. In lamprey, the ascending system consists of spinobulbar neurons which convey spinal network signals to the two descending systems, the reticulospinal and vestibulospinal neurons. Previous studies showed that spinobulbar neurons consist of both ipsilaterally and contralaterally projecting cells distributed at all rostrocaudal levels of the spinal cord, though most numerous near the obex. The axons of spinobulbar neurons ascend in the ventrolateral spinal cord and brainstem to the caudal mesencephalon and within the …

Waterhole Dynamics And Chemical Signals Of African Elephants ( Loxodonta Africana ), Stacie Marie Castelda

College of Graduate Studies: Theses & Dissertations

The distribution of mammals in a savanna environment is influenced by abiotic and biotic factors. The African savanna experiences distinct wet and dry seasons. Species become spatially restricted around water resources during the dry season, resulting in increased intraspecies and interspecies resource-based aggregation. A dominate species, such as the African elephant (Loxodonta africana), may influence this aggregation. I examined the influence of wet and dry seasons and ambient temperature on the use of a waterhole by elephants as well as the co-occurrence of elephants and other mammals at the waterhole. Biodiversity scans (October 2004-June 2007) and were performed at a …

Sensitivity Of Kinetoplastids To Aminoglycoside: Correlation With The 3’ Region Of The Small Subunit Rrna Gene, A. S. Hamad Elgazwy, Masoom M. Yasinzai

Turkish Journal of Biology

In vitro culture systems were used to assess the growth inhibition of kinetoplastids by a variety of aminoglycosides. The parasites were allowed to grow to the stationary phase in the presence of varying concentrations of the drugs. The IC-50 for every drug was calculated by comparison with the control. The sensitivity of various leishmanial strains to these drugs was in the order of: G418 > hygromycin > paromomycin > neomycin; however, under our assay conditions gentamycin and kanamycin were non-leishmanicidal. The effects of the drugs on the intracellular form of the parasite in the macrophage cell line were also tested. Other kinetoplastids, such …

Specific Individuals Of Rainbow Trout (Oncorhynchus Mykiss) Are Able To Show Time-Place Learning, M. Saeed Heydarnejad, G. J. Purser

Turkish Journal of Biology

Using demand-feeders, time-place learning association of food was investigated in rainbow trout (Oncorhynchus mykiss) in 2 small raceways (TR1 and TR2). In order for the fish to be visually identified, they were tagged individually and their behaviour and distribution were monitored by cameras. Equipping with a demand-feeder, TR1 was assigned to test 2 times/1 place (2T/1P) while 2 demand-feeders were used in TR2 assigned to test 2 times/2 places (2T/2P). After 26 days of restricted feeding (RF) the fish were deprived of food for 3 days. The results of the present study indicate that, at the group level, rainbow trout …

The Exploration Of Novel Symbiotic Bacteria That May Have Influential Roles In Sponge Life History, Brittany E. West

Honors Theses

Sponges produce an impressive variety of secondary metabolites that perform a variety of ecological functions. Many marine sponges even harbor diverse carotenoid compounds, an unusual class of secondary metabolites that animals are incapable of producing. Furthermore, sponges serve as hosts to an astonishingly diverse microbial community that can occupy up to sixty percent of a sponge's biomass. Our research ultimately hopes to link microbial species to the production of secondary compounds, like carotenoids, and to assess the ecological role of such compounds and their effect on sponge life history strategy. This study describes the ecological distribution of two species of …

Reproductive Allometry And The Size-Number Trade-Off For Lizards, Eric Charnov, Robin Warne

Biology Faculty & Staff Publications

The yearly reproductive allocation shows a 0.75 allometry with adult size across lizard species.

Medusan Morphospace: Phylogenetic Constraints, Biomechanical Solutions, And Ecological Consequences, John H. Costello, Sean Colin, John O. Dabiri

Arts & Sciences Faculty Publications

Medusae were the earliest animals to evolve muscle-powered swimming in the seas. Although medusae have achieved diverse and prominent ecological roles throughout the world's oceans, we argue that the primitive organization of cnidarian muscle tissue limits force production and, hence, the mechanical alternatives for swimming bell function. We use a recently developed model comparing the potential force production with the hydrodynamic requirements of jet propulsion, and conclude that jet production is possible only at relatively small bell diameters. In contrast, production of a more complex wake via what we term rowing propulsion permits much larger sizes but requires a different …

Qtl Analysis Of Ray Pattern In Caenorhabditis Elegans Recombinant Inbred Lines, Adam Joseph Guess

Browse all Theses and Dissertations

Ray pattern is the most divergent morphological character among rhabditid nematodes. The ancestral ray pattern of Elegans group nematodes is commonly referred to as a 2(1)3+3 pattern, with rays 1 and 2 located anterior of the cloaca, ray 3 positioned at the cloaca, and rays 4-6 and 7-9 grouped in two clusters posterior of the cloaca. C. elegans males primarily exhibit the 2(1)3+3 pattern; however some isolates display variant patterns at a low frequency. The Hawaiian strain CB4856 exhibits a 3/3+3 pattern at a frequency of 0.09. In this pattern, ray 3 is located anterior of the cloaca in a …

Using Geographic Information Systems To Select Sites For Wetland Restoration In West Central Ohio's Agricultural Areas, Janice M. Hartkorn

Browse all Theses and Dissertations

The Mississippi River Basin, includes a major part of Ohio has encountered increasing phosphorus and nitrogen loads from agricultural fields since the 1800's when intensive agriculture moved into the Midwest. Agriculture has drained ninety percent of Ohio's native wetlands. A portion of those drained wetlands can be restored to functional wetlands to intercept excess nutrients from non-productive or low yield agricultural fields to improve overall water quality. Little is known about finding potential restoration sites, partly because of the difficulty in locating sites capable of supporting successful restoration.

This study investigated the utility of Geographic Information Systems (GIS) as a …

Immunomodulation Of Human Skin Cells By Extracts Of The Scabies Mite, Sarcoptes Scabiei, Jeremi Stevan Mullins

Browse all Theses and Dissertations

Sarcoptes scabiei infests its host through burrowing into the stratum corneum layer of skin. Keratinocytes and fibroblasts responding to the burrowing mite(s) and/or mite products produce inflammatory and immune activating cytokines and chemokines. The purpose of this study was to determine if an extract of S. scabiei can modulate cytokine and chemokine expression from keratinocytes and fibroblasts in vitro. Normal human keratinocytes and fibroblasts were exposed to an extract of S. scabiei var. canis with or without various pro-inflammatory stimuli. Cytokine and chemokine expression were measured by enzyme-linked immunosorbent assay (ELISA). Scabies extract increased expression of interleukin-6 (IL-6), growth-related oncogene-α …

The Effects Of Nutritional Supplement, Mass Fx™, On Muscular Strength, Body Composition, And Blood Chemistries In Resistance Trained Adult Males, Patrick S. Dib

Browse all Theses and Dissertations

The purpose of this study was to determine the effects of a nutritional supplement, Mass FX, on muscular strength, body composition, and blood chemistries in resistance-trained adult males. Eight subjects, mean age 25 +/- 3.02 years, were randomly assigned to two groups (n=4). Each group was given either Mass FX or a Placebo in a double-blind manner to be taken orally for six weeks (4caps/day regardless of bodyweight). For the duration of the study, both groups were following the same training program and a diet customized to each subject's bodyweight in conjunction to the supplementation. Data were analyzed using Analysis …

Effects Of Forest Fragmentation And Honeysuckle Invasion On Forest Lepidoptera In Southwest Ohio, Annie Lynn Doyle

Browse all Theses and Dissertations

Habitat loss and exotic species invasion is a rapidly growing threat facing forest animal communities worldwide. The goal of the current study is to assess the impact of forest fragmentation and the associated invasion of honeysuckle on immature tree-feeding Lepidoptera communities in southwestern Ohio. Caterpillar abundance, richness, and honeysuckle density were sampled along 100 meter transects conducted in ten forest fragments. Generalized linear models were developed to determine the effects of fragment area, landscape forest cover, and honeysuckle density on caterpillar abundance and richness. Caterpillar abundance and richness was positively related to landscape forest cover and fragment area. However, these …

The Community Structure Of Methanogenic, Methanotrophic, And Ammonia Oxidizing Bacteria In Vertical Flow Greenhouse Wetland Mesocosms Exposed To Pce, William Evan Gruner

Browse all Theses and Dissertations

Chlorinated ethenes are among the top contaminants on the EPA National Priorities List due to their abundance in contaminated sites and their recalcitrant nature. These compounds, which are used in large scale degreasing and textile processes, are known liver and kidney toxins, and suspected carcinogens. Wetland ecosystems are capable of degrading PCE and its breakdown products TCE, DCE, and VC by providing anaerobic and aerobic growth conditions for microbes that express enzymes such as dehalogenases and monooxygenases with dual affinity for chlorinated solvents. Greenhouse mesocosms at Wright State University are used to mimic field conditions at a treatment wetland at …

Evaluation Of Aerial Cluster Sampling Of Double-Crested Cormorants On Aquaculture Ponds In Mississippi, Brian S. Dorr, Loren W. Burger Jr., Scott C. Barras

United States Department of Agriculture Wildlife Services: Staff Publications

Concern over increasing numbers of double-crested cormorants (Phalacrocorax auritus) and their impacts on channel catfish (Ictalurus punctatus) aquaculture has resulted in increased need for quantitative information to develop and evaluate depredation management efforts. We evaluated aerial surveys in a stratified cluster sampling (SCS) design to estimate and monitor abundance of cormorants on catfish aquaculture ponds in the Yazoo River Basin of Mississippi, USA (hereafter Yazoo Basin). Twice monthly abundance estimates and coefficient of variation during winter averaged 8,128 (n = 29, SE = 1,233) and 33% (n = 29, SE = 0.02), respectively. Counts of cormorants …

The Spectrum Of Mitochondrial Mutation Differs Across Species, Kristi L. Montooth, David M. Rand

School of Biological Sciences: Faculty Publications

Mutations are ubiquitous, and many arise during the very process of replicating and transmitting genomes. This process is the source of the genetic variation that provides the raw material for both evolutionary novelty and human disease. Mutation rates are known to vary among nucleotides, across genomic regions, and between taxa. It is conventional wisdom that animal mitochondrial DNA (mtDNA) is one genomic region that has a particularly high mutation rate. Until recently, this high rate of mutation has been predominantly inferred from high levels of mitochondrial sequence divergence between species. However, the apparently simple process of mutation and sequence divergence …

Oviposition Behavior Partitions Aquatic Landscapes Along Predation And Nutrient Gradients, C. A. Binckley, W. J. Resetarits Jr.

Biological Sciences Faculty Publications

That individuals attempt to minimize the ratio of mortality risk/growth rate (μ/g) when foraging within individual habitat patches is well established. Do species partition among spatially discrete communities embedded in complex landscapes in a similar manner? We investigated how 3 ovipositing species (2 Hyla treefrogs and a hydrophilid beetle, Tropisternus lateralis) responded to simultaneous gradients of nutrients and predation risk. Species partitioned our experimental metacommunity primarily by reducing oviposition with fish. Tropisternus positively responded to increased nutrients, but the effect decreased with increasing risk, as predicted by μ/g theory. Use of fish habitats by Tropisternus was unrelated to breeding …

Swimming Dynamics And Propulsive Efficiency Of Squids Throughout Ontogeny, Ian K. Bartol, Paul S. Krueger, Joseph T. Thompson, William J. Stewart

Biological Sciences Faculty Publications

Synopsis Squids encounter vastly different flow regimes throughout ontogeny as they undergo critical morphological changes to their two locomotive systems: the fins and jet. Squid hatchlings (paralarvae) operate at low and intermediate Reynolds numbers (Re) and typically have rounded bodies, small fins, and relatively large funnel apertures whereas juveniles and adults operate at higher Re and generally have more streamlined bodies, larger fins, and relatively small funnel apertures. These morphological changes and varying flow conditions affect swimming performance in squids. To determine how swimming dynamics and propulsive efficiency change throughout ontogeny, digital particle image velocimetry (DPIV) and kinematic …

Historical Mammal Extinction On Christmas Island (Indian Ocean) Correlates With Introduced Infectious Disease, Kelly B. Wyatt, Paula F. Campos, M. Thomas P. Gilbert, Sergios-Orestis Kolokotronis, Wayne H. Hynes, Rob Desalle, Peter Daszak, Ross D.E. Macphee, Alex D. Greenwood

Biological Sciences Faculty Publications

It is now widely accepted that novel infectious disease can be a leading cause of serious population decline and even outright extinction in some invertebrate and vertebrate groups (e. g., amphibians). In the case of mammals, however, there are still no well-corroborated instances of such diseases having caused or significantly contributed to the complete collapse of species. A case in point is the extinction of the endemic Christmas Island rat (Rattus macleari): although it has been argued that its disappearance ca. AD 1900 may have been partly or wholly caused by a pathogenic trypanosome carried by fleas hosted …

Amp-Activated Protein Kinase Is Involved In Hormone-Induced Mouse Oocyte Meiotic Maturation In Vitro, Jing Chen, Stephen M. Downs

Biological Sciences Faculty Research and Publications

We have previously shown that AMP-activated protein kinase (AMPK) can induce the resumption of meiosis in mouse oocytes maintained in meiotic arrest in vitro. The present study was carried out to determine whether AMPK activation is involved in hormone-induced maturation. Follicle-stimulating hormone (FSH) and the EGF-like peptide, amphiregulin (AR), are potent inducers of maturation in cumulus cell-enclosed oocytes (CEO). Within 3 h of FSH treatment, phospho-acetyl CoA carboxylase (ACC) levels were increased in germinal vesicle (GV)-stage oocytes when compared to non-stimulated controls and remained elevated throughout 9 h of culture, indicating AMPK activation. A similar response to AR was observed …

Foraging Site Selection In Prairie Kingsnakes (Lampropeltis C. Calligaster): An Experimental Approach, Angela M. Fornell

Masters Theses

A critical component to a predator’s survival is the choice of foraging sites that yield enough predatory success to meet energetic demands. To elucidate cues used to select foraging sites, I examined foraging site selection in Prairie Kingsnakes (Lampropeltis c. calligaster), a generalist species whose foraging mode is flexible. My objectives were to determine: the pattern of foraging site selection; the importance of certain cues that kingsnakes use when selecting foraging sites; and, whether or not kingsnakes adjust their foraging patterns in response to alterations in the microhabitat. I tested subjects in a large experimental arena under several treatment conditions …

Terrestrial Macrofungi Of Old-Growth Prairie Groves, Vincent P. Hustad

Masters Theses

The purpose of this study was to evaluate and contrast the communities of terrestrial macrofungi of Brownfield and Trelease Woods, Champaign Co., IL; old-growth prairie groves isolated for> 120 years. This study represents the first known analysis of fungal communities in old-growth prairie grove ecosystems in the Midwestern United States, and provides an excellent opportunity to characterize the fungal biodiversity of established old-growth reference communities for comparisons with other sites, both local and distant. Fungal communities were sampled during the fall fruiting seasons of 2006 and 2007 using a plot frequency study of fungal abundance. Several estimators of species richness …

Leptodactylus Cunicularius Sazima And Bokermann Rabbit-Burrow Frog, W. R. Heyer, M. M. Heyer, Rafael O. De Sá

Biology Faculty Publications

Adult Leptodactylus cunicularius are moderately small. The head is longer than wide and the hind limbs are long (Table 1; Heyer and Thompson 2000 provided definitions of adult size and leg length categories for Leptodactylus). Male vocal sacs are internal, not externally expanded. The snout is protruding, not sexually dimorphic. Male forearms are not hypertrophied and males lack asperities on the thumbs and chest. The dorsum is variegated with small, often confluent, spots and blotches. There is a very thin interrupted mid-dorsal light stripe (pinstripe). Usually, there is a noticeable light, irregular, elongate, mid-dorsal blotch in the scapular region. …

Mating Bias In The Red Imported Fire Ant, Solenopsis Invicta, Kendra Carroll

Masters Theses

The two social forms in the red imported fire ant, Solenopsis invicta, are believed to be governed by the expression of one specific gene. This gene, known as Gp-9, is responsible for the production of general protein-9, thought to be a pheromone binding protein. Among the social aspects thought to be controlled by this locus is reproduction. Many aspects of breeding within and between the two colonial forms have been investigated over the past years since in introduction of this species to the United States. In this study, several features of the currently accepted model were examined. Newlymated queens were …

Spatial And Ecological Patterns Of Tent Location In The Eastern Tent Caterpillar, Malacosoma Americana (Lepidoptera:Lasiocampidae), Jennifer Pleasant

Masters Theses

The eastern tent caterpillar, Malacosoma americanum (Fabricius) (Lepidoptera: Lasiocampidae) is a widely studied social caterpillar. The nests (i.e. tents) of the tent caterpillar are most frequently found on Black Cherry trees (Prunus serotina). However, dispersions of tents are often clumped with one cherry tree having five or more tent caterpillar nests in it and another cherry tree 3 meters away having only one nest, or none at all. I tried to determine why this happens. Because of the short field season, much of the research on the caterpillars has been done in the laboratory. I tried to determine host selection …

A Survey Of Carrion And Burying Beetles (Coleoptera:Silphidae) In Mchenry County, Illinois, David Suhrbur

Masters Theses

This study compared the occurrence of beetles in the family Silphidae using historical collection records with that of field collections from five different sites within McHenry County. The five sites include: Glacial Park, Woodstock, Silver Creek, Rush Creek, and Coral Woods. Historically, 11 known silphid species have been found in McHenry County, compared with seven species found during this study' collection period.

Percentages of silphid species at each site was calculated, as well as total number of individuals sampled, and two indices of diversity (Shannon-Weiner and Simpson's) were applied to both current and historical collection data. The historical collection (when …

Movement Patterns And Feeding Behavior Of The Limpet Tectura Testudinalis (Müller) Along The Mid-Maine Coast, Joshua Lord

Honors Theses

Tectura testudinalis is a limpet that lives in the mid-intertidal zone along the coast of Maine and grazes on a variety of encrusting algae. A previous study asserted that T. testudinalis preferred to feed and rest on the encrusting alga Clathromorphum circumscriptum and that this species of limpet displayed homing behavior. However, I show that T. testudinalis does not home or return to any specific substrate while resting. Conclusive evidence was found for nocturnal movement. I show that C. circumscriptum was the preferred food source for this limpet, closely followed by Hildenbrandia rubra, another encrusting alga. Field and lab experiments …

Prevalence And Severity Of Fibropapillomatosis In Juvenile Green Turtles (Chelonia Mydas) In Three Habitats On Florida's Eas, Kelly Borrowman

Electronic Theses and Dissertations

Fibropapillomatosis (FP) is a tumor-forming disease mainly found in juvenile green turtles (Chelonia mydas) inhabiting Florida's east coast. Despite increased research on the herpes virus that putatively causes it, long-term assessment is still needed of the distribution and severity of FP. Using the decades-long database compiled by the University of Central Florida Marine Turtle Research Group, I determined FP severity and distribution at three different sites: Indian River Lagoon (IRL), Sabellariid Worm Reef (SWR) and Trident Submarine Basin (TSB). Fibropapillomatosis occurred in > 50% of IRL turtles, 18% of SWR turtles and <1% of TSB turtles. Regression of FP tumors was correlated with its prevalence, i.e. when and where FP was common, tumor regression was common. The probability of a turtle being non-afflicted or mildly afflicted increased with both increasing straight-line carapace length (SCL) and increasing relative body condition (residuals of log body mass versus log SCL). Mean annual growth rates of IRL and SWR turtles did not vary with FP severity; however, mean annual growth rates in IRL (1.10 cm/y) were significantly higher than in SWR (1.05 cm/y) and TSB (1.04 cm/y). Annual apparent survival estimates for IRL and TSB turtles were 0.72 and 0.73, respectively, and were constant over time. Even with increased prevalence of FP, annual apparent survival estimates were constant and similar to survival estimates of juvenile green turtles in areas without FP. In IRL, survival rates among FP Categories 0, 1 and 2 were similar (0.74, 0.74 and 0.81 respectively) and lower only for FP Category 3 (0.63). Thus, while FP occurred in > 50% of turtles in some Florida east coast populations, …