Digital Commons Network^™

Apicomplexan Parasites Co-Opt Host Calpains To Facilitate Their Escape From Infected Cells, Rajesh Chandramohanadas, Paul H. Davis, Daniel P. Beiting, Michael B. Harbut, Claire Darling, Geetha Velmourougane, Ming Yeh Lee, Peter A. Greer, David S. Roos, Doron C. Greenbaum

Biology Faculty Publications

Apicomplexan parasites, including Plasmodium falciparum and Toxoplasma gondii (the causative agents of malaria and toxoplasmosis, respectively), are responsible for considerable morbidity and mortality worldwide. These pathogenic protozoa replicate within an intracellular vacuole inside of infected host cells, from which they must escape to initiate a new lytic cycle. By integrating cell biological, pharmacological, and genetic approaches, we provide evidence that both Plasmodium and Toxoplasma hijack host cell calpain proteases to facilitate parasite egress. Immunodepletion or inhibition of calpain-1 in hypotonically lysed and resealed erythrocytes prevented the escape of P. falciparum parasites, which was restored by adding purified calpain-1. Similarly, efficient …

Analysis And Classification Of Sounds Produced By Asian Elephants (Elephas Maximus), Sharon Stuart Glaeser

Dissertations and Theses

Relatively little is known about the vocal repertoire of Asian elephants (Elephas maximus), and a categorization of basic call types and modifications of these call types by quantitative acoustic parameters is needed to examine acoustic variability within and among call types, to examine individuality, to determine communicative function of calls via playback, to compare species and populations, and to develop rigorous call recognition algorithms for monitoring populations.

This study defines an acoustic repertoire of Asian elephants based on acoustic parameters, compares repertoire usage among groups and individuals, and validates structural distinction among call types through comparison of manual and automated …

Isolation And Characterization Of A Spontaneously Immortalized Bovine Retinal Pigmented Epithelial Cell Line, Thomas E. Liggett, T. Daniel Griffiths, Elizabeth R. Gaillard

Biological Sciences Faculty Research and Publications

Background

The Retinal Pigmented Epithelium (RPE) is juxtaposed with the photoreceptor outer segments of the eye. The proximity of the photoreceptor cells is a prerequisite for their survival, as they depend on the RPE to remove the outer segments and are also influenced by RPE cell paracrine factors. RPE cell death can cause a progressive loss of photoreceptor function, which can diminish vision and, over time, blindness ensues. Degeneration of the retina has been shown to induce a variety of retinopathies, such as Stargardt's disease, Cone-Rod Dystrophy (CRD), Retinitis Pigmentosa (RP), Fundus Flavimaculatus (FFM), Best's disease and Age-related Macular Degeneration …

Thyroid Hormone Regulation Of Mrnas Encoding Thyrotropin Β-Subunit, Glycoprotein Α-Subunit, And Thyroid Hormone Receptors Α And Β In Brain, Pituitary Gland, Liver, And Gonads Of An Adult Teleost, Pimephales Promelas, Sean C. Lema, Jon T. Dickey, Irvin R. Schultz, Penny Swanson

Biological Sciences

Thyroid hormones (THs) regulate growth, morphological development, and migratory behaviors in teleost fish, yet little is known about the transcriptional dynamics of gene targets for THs in these taxa. Here, we characterized TH regulation of mRNAs encoding thyrotropin subunits and thyroid hormone receptors (TRs) in an adult teleost fish model, the fathead minnow (Pimephales promelas). Breeding pairs of adult minnows were fed diets containing 3,5, 3^,-triiodo-_L-thyronine (T₃) or the goitrogen methimazole for 10 days. In males and females, dietary intake of exogenous T₃ elevated circulating total T₃, while methimazole …

The Effect Of Breeding Herd Parity Structure On Genetic Improvement Of The Sow Herd, Caitlyn E. Abell

Mahurin Honors College Capstone Experience/Thesis Projects

This study focuses on the value of the genetic lag associated with maintaining sows for additional parities in a commercial swine herd. Three traits were included in this study: number born alive (NBA), 21 day litter weight (W21), and days to market (D250). The economic values assigned to these traits were $22.00/pig, $0.70/lb., and $0.17/day, respectively. The genetic improvement per generation made for each trait was assumed to be 0.3 pigs, 3.0 lbs., and 3.0 days, respectively. It was estimated that the value of the genetic lag associated with retaining a sow to P3, P5, and P7 was $24.80, $46.89, …

Common Garden Comparisons Of Native And Introduced Plant Populations: Latitudinal Clines Can Obscure Evolutionary Inferences, Robert I. Colautti, John L. Maron, Spencer C.H. Barrett

Biological Sciences Faculty Publications

Common garden studies are increasingly used to identify differences in phenotypic traits between native and introduced genotypes, often ignoring sources of among-population variation within each range. We re-analyzed data from 32 common garden studies of 28 plant species that tested for rapid evolution associated with biological invasion. Our goals were: (i) to identify patterns of phenotypic trait variation among populations within native and introduced ranges, and (ii) to explore the consequences of this variation for how differences between the ranges are interpreted. We combined life history and physiologic traits into a single principal component (PC_ALL) and also compared …

Do Hypothermic Tissue Tolerances Limit Torpor Expression?, Clark J. Cotton, Henry J. Harlow

Biology Faculty Publications

1. Arrest temperatures and Q₁₀ values for extensor digitorum longus (EDL), soleus, trabecula, and jejunum muscle twitch strength, contraction time, and 0.5 relaxation time were calculated for a deep torpor hibernator, white-tailed prairie dog (WTPD) (Cynomys leucurus), a shallow torpor hibernator, black-tailed prairie dog (BTPD) (Cynomys ludovicianus), and a non-hibernator, lab rat (Rattus norvegicus) to test the hypothesis that tissue temperature tolerances limit the depth of expressed torpor.

2. There were no temperature tolerance differences between the tissues of the two species of hibernators. Both hibernating species had arrest temperatures and Q_{10 …}

Seed Banks Of Sagebrush Communities Seeded With Crested Wheatgrass, Kevin L. Gunnell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Crested wheatgrass (Agropyron cristatum [L.] Gaertn.) is one of the most commonly seeded exotic species in the western United States. Although many degraded Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis) plant communities have been seeded with crested wheatgrass during rehabilitation efforts, seed banks of these communities have not been characterized. I sought to characterize and explain the variation among 33 seeded communities in the northeastern Great Basin. Hierarchical clustering and principal components analysis identified four possible seed bank categories in these communities. Seed bank categories varied from high to low crested wheatgrass dominance. The crested wheatgrass-dominated category …

An Investigation Of Postzygotic Reproductive Isolation And Phenotypic Divergence In The Bark Beetle Dendroctonus Ponderosae, Ryan R. Bracewell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Understanding reproductive isolation and divergence is the focus of speciation research. Recent evidence suggested that some Dendroctonus ponderosae populations produced hybrids with reproductive incompatibilities, a reproductive boundary undetected by phylogeographic analyses using molecular markers. Additionally, the unique bifurcated distribution of D. ponderosae and the proposed isolation-by-distance gene flow pattern around the Great Basin Desert provided a unique opportunity to investigate the evolution of postmating (postyzygotic) isolation while also understanding phenotypic divergence along latitudinal (climatic) gradients. First, I characterized the strength, biological pattern, and geographic pattern of postzygotic isolation in D. ponderosae by crossing increasingly divergent populations in a common garden …

Consumer-Driven Nutrient Recycling In Arctic Alaskan Lakes: Controls, Importance For Primary Productivity, And Influence On Nutirient Limitation, Cody R. Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In lakes, fish and zooplankton can be both sources and sinks of nitrogen (N) and phosphorus (P) through the consumption of organic N and P, and subsequent excretion of bioavailable inorganic forms. These source/sink dynamics, known as consumer-driven nutrient recycling (CNR), may, in turn, control the availability of potentially limiting nutrients for algal primary production. In this dissertation I investigate the importance and controls of CNR as a source of inorganic N and P for primary production (Chapter 2). I then examine zooplankton CNR as a mechanism for increasing nutrient mean resident time (MRT) in the mixed layer of lakes …

Mechanisms Of Induced Cell Death In Bluetongue Virus Challenged Human Cell Lines, Justin Darrel Hoopes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bluetongue virus (BTV) is a pathogenic member of the Reoviridae family. BTV does not cause disease in humans, but is capable of selectively infecting and killing certain transformed human cell lines. Understanding BTV's oncotrophism may lead to new therapeutics for treating cancer. This study focused on the underlying mechanisms of BTV-induced cell death in carcinoma cell lines. It was our hypothesis that BTV infects human carcinoma transformed cells, produces mRNA and protein, induces a strong inflammatory response, induces mitogen activated protein kinase (MAPK)-based pro-apoptotic signaling, inhibits PKB-based signaling, and eventually kills the cell by inducing apoptosis.

Three carcinoma cell lines …

Consequences Of Vegetation Change On The Dynamics Of Labile Organic Matter And Soil Nitrogen Cycling In A Semiarid Ecosystem, Toby D. Hooker

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sagebrush-dominated ecosystems are being transformed by wildfire, rangeland improvement techniques, and exotic plant invasions. These disturbances have substantial effects on the composition and structure of native vegetation, but the effects on ecosystem C and N dynamics are poorly understood. To examine whether differences in dominant vegetation affect the quantity and quality of plant organic matter inputs to soil, ecosystem C and N pools and rates of plant turnover were compared among historically grazed Wyoming big sagebrush, introduced perennial crested wheatgrass, and invasive annual cheatgrass communities. Since low soil moisture during the summer may inhibit the microbial colonization of plant detrital …

The Regulation Of Epithelial Sodium Channels In Mammalian Taste Receptor Cells, Arian F. Baquero Gonzalez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nutrient recognition is one of the main physiological roles of the gustatory system. In mammals, it is well established that the taste of sodium salts is primarily mediated by sodium influx through the epithelial sodium channel. The epithelial sodium channel is a sodium-specific ion channel that is expressed across a wide range of transporting epithelia such as colon, kidney, and taste. In addition to its role as a salt taste receptor, sodium influx through the epithelial sodium channel is important systemically for maintaining sodium balance and blood pressure. Following our earlier work on the endocrine regulation of salt taste at …

Targeting Fat-Sensitive Pathways In Enteroendocrine Cells Using Nanoparticle-Mediated Drug Delivery, Bhavik P. Shah

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The current epidemic of obesity has been linked to an increase in fat intake associated with the Western diet. Nutrient-induced stimulation of enteroendocrine cells in the small intestine leads to the release of hormones that contribute to satiety and the control of food intake. In particular, ingested fat, specifically in the form of free fatty acids, is potent activator of enteroendocrine cells in the proximal small intestine. However, the underlying signaling cascade that free fatty acids initiate in these enteroendocrine cells, which leads to secretion of satiety hormones, is not known. In general, my research is focused on identifying nutrient-responsive …

Punta Toro Virus Infection In Mice: Strain Differences In Pathogenesis And Regulation Of Interferon Response Pathways, Michelle Mendenhall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Adames strain of Punta Toro virus (PTV-A) causes acute hepatic disease in hamsters and mice similar to that seen in natural Rift Valley fever virus (RVFV) infection, while the Balliet strain (PTV-B) is apathogenic. The ability of PTV-A to suppress the interferon (IFN) response has been demonstrated in hamsters and is thought to be a contributing factor to PTV-A's pathogenicity in hamsters. PTV-B is not assumed to exhibit this IFN-antagonistic activity, as it stimulates production of significantly higher IFN-β levels. To elucidate the role of IFN in resistance of mice to PTV-B infection, we utilized mice deficient in a …

Inducing A Normal Phenotype In Breast Epithelial Cells Using A Three-Dimensional Basement Membrane Extract Culture System: A Study On The Reversion Of Cancer, Ross H. Booth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Experimentally, traditional developmental models and transgenic animals consistently underscore the importance of studying cell behavior in the correct tissue context. However, live animal experimentation is inherently complex, and systematic assessment of the effects of individual variables, such as cell shape and matrix compliance on cell behavior, is extremely difficult at best. Two-dimensional monolayer culture of key individual cell types has provided abundant, fundamental information on cell response, but cannot be used to show the normal phenotype of breast epithelial cells. Furthermore, their results often fail to translate into in vivo and clinical studies. It has been previously established that normal …

The Use Of Reverse Genetics To Clone And Rescue Infectious, Recombinant Human Parainfluenza Type 3 Viruses, Jason Peter Roth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Reverse genetics is a discipline that involves the use of genetic manipulation and modification to study an organism's altered phenotype. In this study, infectious recombinant viruses were rescued from altered cDNA clones encoding the antigenome of human parainfluenza virus type 3 and the resulting phenotypes were examined. In one clone, the gene for the enhanced green fluorescent protein was inserted into the virus antigenome to be expressed during viral replication, resulting in infected cells emitting green fluorescence. Viral titers, mRNA replication, and genomic replication for the virus expressing the enhanced green fluorescent protein were reduced when compared to the human …

Greater Sage-Grouse And Energy Development In Northeastern Utah: Implications For Management, Leah Suzanne Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Concern regarding the effect of energy development on greater sage-grouse (Centrocercus urophasianus) is increasing as the search for fossil fuel intensifies. Sage-grouse may be especially sensitive to energy development because they require large, diverse areas of sagebrush (Artemisia spp.) habitat to complete their life cycle. Additionally, the network of pipelines, roads, and wells required by energy development may fragment sagebrush habitat isolating populations and contributing to genetic drift, inbreeding, local extinction, or rapid divergence.

Seep Ridge, located in northeastern Utah, is one area where sage-grouse habitat and energy development plans overlap. Approved leases call for the construction …

Application Of Electrified Fladry To Decrease Risk Of Livestock Depredation By Wolves (Canis Lupus), Nathan J. Lance

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wolf (Canis lupus) predation on livestock can cause economic and emotional hardships for livestock producers, complicating the balance of wolf conservation with other human interests. New management tools that decrease risk of predation may offer additional flexibility or efficiency for both livestock producers and management agencies. I examined 1) the efficacy of electrified fladry compared to fladry at protecting a food source from wolves in captivity, 2) the efficacy of electrified fladry for reducing wolf use of pastures and preventing depredations, and 3) the applicability of electrified-fladry. In captivity I tested the reaction from 15 groups (46 wolves) …

Evaluation Of Macroinvertebrates As A Food Resource In The Assessment Of Lotic Salmonid Habitat, Nicholas P. Weber

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Criteria used to characterize lotic salmonid habitat are often based on observed correlations between physical habitat characteristics and salmonid abundances. A focus on physical habitat features ignores other habitat components, such as an adequate supply of food that set the physiological limitations on salmonid growth and survival. This study outlines the development of a habitat assessment approach that focuses on how invertebrate food availability interacts with stream temperatures to determine salmonid growth potentials. Abundances of benthic and drifting invertebrate communities, stream temperatures, and juvenile steelhead trout (Onchorhynchus mykiss gairdneri) summer growth rates and abundances were measured within 10 …

Surgical Sterilization Of Coyotes To Reduce Predation On Pronghorn Fawns, Renee Seidler

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Coyote (Canis latrans) predation accounts for the majority of neonatal pronghorn (Antilocapra americana) mortality in many areas and may influence local population declines. Current techniques used to manage coyote predation on wildlife species generally focus on lethal control methods. However, these methods may be controversial to the general public. Coyote sterilization is an alternative predation control method which is more acceptable to the public and has been shown to be effective in reducing sheep predation. We hypothesized that surgical sterilization of coyotes may increase pronghorn fawn survival; in the same way it reduces coyote predation on …

Linking Space Use And Behavior In Clark's Nutcracker: An Explanation For Seasonal Variation In Space Use, Teresa J. Lorenz

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Clark's Nutcrackers (Nucifraga columbiana) are important seed dispersers for at least 10 species of conifer in western North America and are obligate mutualists for the subalpine tree, whitebark pine (Pinus albicaulis). Despite the important role they play in forest regeneration, space use by nutcrackers has not been formally studied. Several hypotheses exist to explain their year-round space use patterns. I tested one hypothesis that Clark's Nutcrackers migrate altitudinally between summer and autumn in one population in the Cascade Range, Washington. In 2006 and 2007, I compared seasonal differences in summer and autumn space use by 26 …

Speciation, Species Concepts, And Biogeography Illustrated By A Buckwheat Complex (Eriogonum Corymbosum), Mark W. Ellis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The focus of this research project is the complex of infraspecific taxa that make up the crisp-leaf buckwheat species Eriogonum corymbosum (Polygonaceae), which is distributed widely across southwestern North America. This complex provides an ideal taxonomic group for research into population relationships and speciation. To avoid unnecessary debates about taxonomic validity or contentious issues regarding appropriate species definitions, the historical evolution of the species concept is first reviewed in detail, demythologizing an often-assumed species problem. Following that review, the E. corymbosum complex is examined specifically.

Although eight varieties of E. corymbosum are currently recognized based on morphological characters, this group …

The Four-And-A-Half Lim Domain Protein 2 Regulates Vascular Smooth Muscle Phenotype And Vascular Tone, Nicole A. Neuman, Susan Ma, Gavin R. Schnitzler, Yan Zhu, Giorgio Lagna, Akiko Hata

Biology

In responsetovascular injury, differentiated vascular smooth muscle cells (vSMCs) undergo a uniqueprocess knownas“phenotype modulation,” transitioning from a quiescent, “contractile” phenotype to a proliferative, “synthetic” state. We have demonstrated previously that the signaling pathway of bone morphogenetic proteins, members of the transforming growth factor family, play a role in the induction and maintenance of a contractile phenotype in human primary pulmonary artery smooth muscle cells. In this study, we show that a four-and-ahalf LIM domainprotein 2(FHL2)inhibits transcriptional activation ofvSMC-specificgenesmediatedbythebonemorphogenetic protein signaling pathway through the CArG box-binding proteins, such as serum response factor and members of the myocardin (Myocd) family. Interestingly, FHL2 …

The Effects Of Stream Discharge And Channelization On The Macroinvertebrate Community In A Semi-Arid Landscape, Thomas L. Kennedy

Biology ETDs

The Middle Rio Grande is the second largest watershed in the southwestern United States and is a vitally important component for the biodiversity in the region. Similar to many other rivers, the MRG is a temporally dynamic system that unfortunately, has been subjected to river regulation. As a result, the river has become channelised in many reaches disconnecting it from the surrounding floodplain. The effects of river regulation on the MRG and its surrounding riparian zone were largely unknown. I tested three main hypothesis with regards to the macroinvertebrates in the MRG: 1) Does flow variability structure the aquatic macroinvertebrate …

Controls On Microbial Community Structure In Thermal Environments: Exploring Bacterial Diversity And The Relative Influence Of Geochemistry And Geography, Kendra Renee Mitchell

Biology ETDs

Community wide molecular surveys have revealed incredible hidden phylogenetic and metabolic diversity in microbial habitats. We have conducted the first microbial survey of Yellowstone National Park thermal environments, sampling 103 communities from across the park and across the range of conditions found. Yellowstone is particularly suited for this type of research because of the large number and wide variety of thermal springs, which are naturally occurring chemostats enabling examination of the factors that control microbes and drive community structure. In addition to samples for molecular microbial analysis, we collected water for extensive geochemical analysis in order to begin to deduce …

Oxidative Stress Induced Hu Protein Expression In Deinococcus Radiodurans, Cara Bevinetto

Honors Capstones

No abstract provided.

Cytokine Production During Microsporidia Infection Of Macrophages, Anne Louise Hotard

Honors Capstones

No abstract provided.

Cytokine Production During Microsporidia Infection Of Macrophages, Anne Hotard

Honors Capstones

No abstract provided.

Conservation Genetics Of Boelen‟S Python (Morelia Boeleni) From New Guinea: Reduced Genetic Diversity And Divergence Of Captive And Wild Animals, Joshua Lee Jordan

Honors Capstones

No abstract provided.