Digital Commons Network^™

Linking Snake Behavior To Nest Predation In A Midwestern Bird Community, Patrick J. Weatherhead, Gerardo L.F. Carfagno, Jinelle H. Sperry, Jeffrey D. Brawn, Scott K. Robinson

Biology Faculty Publications

Nest predators can adversely affect the viability of songbird populations, and their impact is exacerbated in fragmented habitats. Despite substantial research on this predator-prey interaction, however, almost all of the focus has been on the birds rather than their nest predators, thereby limiting our understanding of the factors that bring predators and nests into contact. We used radiotelemetry to document the activity of two snake species (rat snakes, Elaphe obsoleta; racers, Coluber constrictor) known to prey on nests in Midwestern bird communities and simultaneously monitored 300 songbird nests and tested the hypothesis that predation risk should increase for nests when …

Grassland Root Communities: Species Distributions And How They Are Linked To Aboveground Abundance., Douglas Frank, Alyssa Pontes, Eleanor M. Maine, Julie Caruana, Ramesh Raina, Surahbi Raina, Jason Fridley

Biology - All Scholarship

There is little comprehensive information on the distribution of root systems among coexisting species, despite the expected importance of those distributions in determining the composition and diversity of plant communities. This gap in knowledge is particularly acute for grasslands, which possess large numbers of species with morphologically indistinguishable roots. In this study we adapted a molecular method, fluorescent fragment length polymorphism, to identify root fragments and determine species root distributions in two grasslands in Yellowstone National Park. Aboveground biomass was measured and soil cores (2 cm diam) were collected to 40 cm and 90 cm in an upland, dry grassland …

A Candidate Subspecies Discrimination System Involving A Vomeronasal Receptor Gene With Different Alleles Fixed In M. M. Domesticus And M. M. Musculus, Robert C. Karn, Janet M. Young, Christina M. Laukaitis

Scholarship and Professional Work - LAS

Assortative mating, a potentially efficient prezygotic reproductive barrier, may prevent loss of genetic potential by avoiding the production of unfit hybrids (i.e., because of hybrid infertility or hybrid breakdown) that occur at regions of secondary contact between incipient species. In the case of the mouse hybrid zone, where two subspecies of Mus musculus (M. m. domesticus and M. m. musculus) meet and exchange genes to a limited extent, assortative mating requires a means of subspecies recognition. We based the work reported here on the hypothesis that, if there is a pheromone sufficiently diverged between M. m. domesticus and …

Combined Effects Of A Parasite, Qpx, And The Harmful-Alga, Prorocentrum Minimum On Northern Quahogs, Mercenaria Mercenaria, Hélène Hégaret, Roxanna Smolowitz, Inke Sunila, Sandra E. Shumway, Jennifer Alix, Mark Dixon, Gary H. Wikfors

Arts & Sciences Faculty Publications

Northern quahogs, Mercenaria mercenaria (L.), frequently are infected with the parasite Quahog Parasite Unknown (QPX, Labyrintohomorpha, Thraustochytriales), which can cause morbidity and mortality of the quahogs. Possible interactions between this parasitic disease and exposure to the harmful dinoflagellate Prorocentrum minimum in M. mercenaria were studied experimentally. Quahogs from Massachusetts with variable intensity of QPX infection were exposed, under controlled laboratory conditions, to cultured P. minimum added to the natural plankton at a cell density equivalent to a natural bloom. After 5 days of exposure, individual clams were diagnosed histologically to assess prevalence and intensity of parasitic infection, as well as …

Two New Species Of Freshwater Crabs From Mt. Elgon, East Africa With A Redescription Of Potamonautes Niloticus And Notes On Their Natural History" , Neil Cumberlidge, Paul F. Clark

Journal Articles

No abstract provided.

Effects Of Flight Speed Upon Muscle Activity In Hummingbirds, Bret W. Tobalske, Andrew A. Biewener, Douglas R. Warrick, Tyson L. Hedrick, Donald R. Powers

Faculty Publications - Department of Biological & Molecular Science

Hummingbirds have the smallest body size and highest wingbeat frequencies of all flying vertebrates, so they represent one endpoint for evaluating the effects of body size on sustained muscle function and flight performance. Other bird species vary neuromuscular recruitment and contractile behavior to accomplish flight over a wide range of speeds, typically exhibiting a Ushaped curve with maxima at the slowest and fastest flight speeds. To test whether the high wingbeat frequencies and aerodynamically active upstroke of hummingbirds lead to different patterns, we flew rufous hummingbirds (Selasphorus rufus, 3��g body mass, 42��Hz wingbeat frequency) in a variable-speed wind tunnel (0–10��m��s–1). …

Arthropod Foraging By A Southeastern Arizona Hummingbird Guild, Don Powers, Jessamyn A. Van Hook, Elizabeth A. Sandlin, Todd J. Mcwhorter

Faculty Publications - Department of Biological & Molecular Science

We tested the hypothesis that foraging for arthropods may be a viable source of energy when hummingbirds are competitively excluded from sources of nectar. We hypothesized that the Magnificent Hummingbird (Eugenes fulgens) relies more upon arthropods than the Blue-throated Hummingbird (Lampornis clemenciae) or Blackchinned Hummingbird (Archilochus alexandri) in southeastern Arizona. We were unable to quantify arthropod foraging by A. alexandri, but measured frequent arthropod foraging by both E. fulgens and L. clemenciae. E. fulgens engaged in more aerial flycatching than L. clemenciae, and their rate of flycatching attempts was higher than by L. clemenciae. Analysis of gut contents showed that …

Mitochondrial Physiology Of Diapausing And Developing Embryos Of The Annual Killifsh Austrofundulus Limnaeus: Implications For Extreme Anoxia Tolerance, Jeff Duerr, Jason E. Podrabsky

Faculty Publications - Department of Biological & Molecular Science

Diapausing embryos of the annual killiWsh Austrofundulus limnaeus have the highest reported anoxia tolerance of any vertebrate and previous studies indicate modified mitochondrial physiology likely supports anoxic metabolism. Functional mitochondria isolated from diapausing and developing embryos of the annual killifish exhibited VO2, respiratory control ratios (RCR), and P:O ratios consistent with those obtained from other ectothermic vertebrate species. Reduced oxygen consumption associated with dormancy in whole animal respiration rates are correlated with maximal respiration rates of mitochondria isolated from diapausing versus developing embryos. P:O ratios for developing embryos were similar to those obtained from adult liver, but were diminished in …

Genetic Conflict And Sex Chromosome Evolution, Colin D. Meiklejohn, Yun Tao

School of Biological Sciences: Faculty Publications

Chromosomal sex determination systems create the opportunity for the evolution of selfish genetic elements that increase the transmission of one sex chromosome at the expense of its homolog. Because such selfish elements on sex chromosomes can reduce fertility and distort the sex ratio of progeny, unlinked suppressors are expected to evolve, bringing different regions of the genome into conflict over the meiotic transmission of the sex chromosomes. Here we argue that recurrent genetic conflict over sex chromosome transmission is an important evolutionary force that has shaped a wide range of seemingly disparate phenomena including the epigenetic regulation of genes expressed …

Effect Of Ingestion By Alligator Snapping Turtles (Macrochelys Temminckii) On Seeds Of Riparian Vegetation, Jean Pierre Elbers

Graduate Theses/Dissertations

The alligator snapping turtle (Macrochelys temminckii) is a large freshwater turtle that inhabits many lotic water bodies in the Southeastern United States. The species consumes primarily fish but also consumes large amounts of vegetation including seeds of common persimmon (Diospyros virginiana), tupelos (Nyssa sp.), willow oak (Quercus phellos), and pecan (Carya illinoensis). Captive specimens of M. temminckii were fed samples of the above-mentioned seeds to assess how the species affects ingested seeds in order to evaluate the potential role this species may play as a seed disperser. The proportion of seeds defecated intact varied with species (57−99 %), was lowest …

Identification Of A Nuclear Export Signal In The Catalytic Subunit Of Amp-Activated Protein Kinase, N. Kazgan, Tyisha Williams, L. J. Forsberg, J. E. Brenman

Biology Faculty Research

The metabolic regulator AMP-activated protein kinase (AMPK) maintains cellular homeostasis through regulation of proteins involved in energy-producing and -consuming pathways. Although AMPK phosphorylation targets include cytoplasmic and nuclear proteins, the precise mechanisms that regulate AMPK localization, and thus its access to these substrates, are unclear. We identify highly conserved carboxy-terminal hydrophobic amino acids that function as a leptomycin B–sensitive, CRM1-dependent nuclear export sequence (NES) in the AMPK catalytic subunit (AMPKα). When this sequence is modified AMPKα shows increased nuclear localization via a Ran-dependent import pathway. Cytoplasmic localization can be restored by substituting well-defined snurportin-1 or protein kinase A inhibitor (PKIA) …

Comparing The Genome Expression Profiles Of Verrucomicrobium Sp. Strain Tav2 Cells Grown Under Two Different Oxygen Concentrations, Austin Willis

Biology Theses - Archive

Termites are significantly different in their lifestyles and organization but most are linked by the common trait in degrading plant material, whether it is leaf litter, wood, soil or animal waste. They consume the cellulose, hemicelluloses and lignin that make up the plant material but rely on a food web consisting of about 270 different phylotypes of microorganisms to break down the biomass into usable nutrients. Isolated from the hindgut of the termite Reticulitermis flavipes, one such organism is Verrucomicrobium sp. strain TAV2. Members of this relatively new bacterium phylum have rigid peptidoglycan with some possessing prosthecae. They have been …

Insights Into The Transmission Of Helitrons And Their Impact On The Genome Architecture Of Myotis Lucifugus, The Little Brown Bat, Jainy Thomas

Biology Dissertations - Archive

Probably the most dynamic proportion of multicellular eukaryotic genomes is composed of teeming populations of parasitic mobile DNA, called transposable elements (TEs). Helitrons are rolling circle TEs that have a limited distribution among mammals, having been identified only in the genome of the little brown bat, Myotis lucifugus, and a few closely related species. One family of Helitrons, called Helibat, was estimated to make up as much as 3% of the M. lucifugus genome. In addition, Helibat was shown to have captured and amplified the promoter and 5' UTR of a highly conserved single copy gene to >1000 copies. Together …

First Report Of The Developmental Changes Inside The Eggs Of The Chinese Freshwater Crab , J Xue, Y Liu, Neil Cumberlidge, H X. Wu

Journal Articles

No abstract provided.

Integrating Spatial And Temporal Approaches To Understanding Species Richness, Ethan P. White, S. K. Morgan Ernest, Peter B. Adler, Allen H. Hurlbert, S. Kathleen Lyons

School of Biological Sciences: Faculty Publications

Understanding species richness patterns represents one of the most fundamental problems in ecology. Most research in this area has focused on spatial gradients of species richness, with a smaller area of emphasis dedicated to understanding the temporal dynamics of richness. However, few attempts have been made to understand the linkages between the spatial and temporal patterns related to richness. Here, we argue that spatial and temporal richness patterns and the processes that drive them are inherently linked, and that our understanding of richness will be substantially improved by considering them simultaneously. The species–time–area relationship provides a case in point: successful …

Fabrication Of Miniature Drug Screening Platform Using Low Cost Bioprinting Technology, Jorge Ivan Rodriguez

Open Access Theses & Dissertations

In the pharmaceutical industry, new chemicals and substances are being tested to find appropriate compounds or mix for treating a specific disease. The demand for screening large compound collections against and increasing number of therapeutic targets has stimulated technology development in the areas of assay automation and miniaturization. Current methods for evaluating the reactions of cells use a volume in the range of microliters. We have developed a new and low-cost deposition method to fabricate miniature drug screening platform that can realistically and inexpensively evaluate biochemical reactions up to 4 substances per trial in a picoliter-scale volume. This paper describes …

Spatial Segregation Of The Sexes In A Salt Marsh Grass Distichlis Spicata (Poaceae), Charlene Ashley Mercer

Dissertations and Theses

Understanding the maintenance of sexual systems is of great interest to evolutionary and ecological biologists because plant systems are extremely varied. Plant sexual systems have evolved to include not only complete plants with both male and female reproduction occurring on one plant (i.e., monoecious and hermaphroditic) but also plants with male and female function on separate plants (dioecious). The dioecious reproductive system can be used to test theories on niche differentiation given that having separate plants potentially allows for the exploitation of a broader niche. This increase in the realized niche is due to the ability for separate sexes to …

The Combined Influence Of The Local Environment Andregional Enrichment On Bird Species Richness, Ethan P. White, A. H. Hurlbert

Biology Faculty Publications

It is generally accepted that local species richness at a site reflects the combined influence of local and regional processes. However, most empirical studies evaluate the influence of either local environmental variables or regional enrichment but not both simultaneously. Here we demonstrate the importance of combining these processes to understand continental scale richness patterns in breeding birds. We show that neither regional enrichment nor the local environment in isolation is sufficient to characterize observed patterns of species richness. Combining both sets of variables into a single model results in improved model fit and the removal of residual spatial autocorrelation. At …

Loss Of Foundation Species Increases Population Growth Of Exotic Forbs In Sagebrush Steppe, J S. Prevey, M J. Germino, Nancy J. Huntly

Biology Faculty Publications

The invasion and spread of exotic plants following land disturbance threatens semiarid ecosystems. In sagebrush steppe, soil water is scarce and is partitioned between deeprooted perennial shrubs and shallower-rooted native forbs and grasses. Disturbances commonly remove shrubs, leaving grass-dominated communities, and may allow for the exploitation of water resources by the many species of invasive, tap-rooted forbs that are increasingly successful in this habitat. We hypothesized that exotic forb populations would benefit from increased soil water made available by removal of sagebrush, a foundation species capable of deep-rooting, in semiarid shrub-steppe ecosystems. To test this hypothesis, we used periodic matrix …

Multiple Endpoints Of Endocrine Disruption In Gravid Watersnakes (Colubridae:Nerodia) As A Function Of Ingestion Of A Common Herbicide, Lorin Anne Neuman-Lee

Masters Theses

Ecotoxicological studies that focus on a single endpoint might not accurately represent the true ecological effects of a contaminant. Exposure to atrazine, a widelyused herbicide, disrupts endocrine function and sexual development in amphibians, but studies involving reptilian species are lacking. This study examines several effects of atrazine ingestion on gravid females and neonates exposed in utero of the Northern Watersnake (Nerodia sipedon). Twenty-five gravid N. sipedon were collected and kept in the lab for the entirety of their gestation period. Each snake received one of four doses of atrazine: control, 2, 20, or 200 ppb. Blood samples were drawn each …

A Spatially Explicit Model To Predict Radiocesium Body Burdens In White-Tailed Deer On The Department Of Energy's Savannah River Site, Christopher W. Bobryk

Masters Theses

A spatially explicit exposure model was developed to interpolate and predict radiocesium (137Cs) body burdens found in white-tailed deer (Odocoileus virginianus) on the U.S. Department of Energy's Savannah River Site (SRS) in west-central South Carolina. Since 1965 hunting has been allowed from permanent stands as a mechanism to manage the herd and all animals have been monitored in the field for gross beta/gamma activity levels providing a long-term spatially explicit dataset. Until now no study has attempted to model deer 137Cs at the hunt-stand level, but rather at the compartment level (1,000 - 5,200 ha). The models described here use …

Prey Preference As A Function Of Feeding Experience And Prey Type In Neonate Gartersnakes (Colubridae: Thamnophis), Kathryn B. Hale

Masters Theses

Several species of potential prey have been introduced into the geographic range of the Two-striped Gartersnake, Thamnophis hammondii. Some of these prey species possess defenses that present novel challenges to the snake predator. Despite novel predator defense mechanisms (including secretions from skin glands), adult T. hammondii ingest several of these prey species without adverse effects. Although ~dult T hammondii are dietary generalists, almost nothing is known about prey preferences in newborn individuals that lack feeding experience. My study determined whether or not T. hammondii innately exhibits a preference among native and introduced prey types. Furthermore, I compared the degree of …

Introduction: Protistan Biology, Horizontal Gene Transfer, And Common Descent Uncover Faulty Logic In Intelligent Design, Avelina Espinosa

Arts & Sciences Faculty Publications

THE International Society of Protistologists (ISOP) organized a pre-meeting workshop entitled ‘‘Horizontal Gene Transfer and Phylogenetic Evolution Debunk Intelligent Design,’’ as part of the 1st North American Section meeting held June 11–13, 2009, at Roger Williams University, Bristol, RI, USA. This workshop focused on the acceptance of Darwinian evolution in the United States and the role of intelligent design (ID) in the ongoing controversy between scientific knowledge and popular belief. Intelligent design, a doctrine born in the 1980s, proposes that a ‘‘Designer’’ is responsible for the complexity in biological systems and that Darwinism cannot explain holistically the origin and evolution …

Integrating Horizontal Gene Transfer And Common Descent To Depict Evolution And Contrast It With ‘‘Common Design, Guillermo Paz-Y-Mino C., Avelina Espinosa

Arts & Sciences Faculty Publications

Horizontal gene transfer (HGT) and common descent interact in space and time. Because events of HGT co-occur with phylogenetic evolution, it is difficult to depict evolutionary patterns graphically. Tree-like representations of life’s diversification are useful, but they ignore the significance of HGT in evolutionary history, particularly of unicellular organisms, ancestors of multicellular life. Here we integrate the reticulated-tree model, ring of life, symbiogenesis whole-organism model, and eliminative pattern pluralism to represent evolution. Using Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a bifunctional enzyme in the glycolytic pathway of amoeba, we illustrate how EhADH2 could be the product of both horizontally acquired …

Bacterial Acquisition In Juveniles Of Several Broadcast Spawning Coral Species, Koty H. Sharp, Kim B. Ritchie

Arts & Sciences Faculty Publications

Coral animals harbor diverse microorganisms in their tissues, including archaea, bacteria, viruses, and zooxanthellae. The extent to which coral-bacterial associations are specific and the mechanisms for their maintenance across generations in the environment are unknown. The high diversity of bacteria in adult coral colonies has made it challenging to identify species-specific patterns. Localization of bacteria in gametes and larvae of corals presents an opportunity for determining when bacterial-coral associations are initiated and whether they are dynamic throughout early development. This study focuses on the early onset of bacterial associations in the mass spawning corals Montastraea annularis, M. franksi, M. faveolata, …

Stealth Predation And The Predatory Success Of The Invasive Ctenophore Mnemiopsis Leidyi, Sean Colin, John H. Costello, Lars J. Hansson, Josefin Titelman, John O. Dabiri

Arts & Sciences Faculty Publications

In contrast to higher metazoans such as copepods and fish, ctenophores are a basal metazoan lineage possessing a relatively narrow set of sensory-motor capabilities. Yet lobate ctenophores can capture prey at rates comparable to sophisticated predatory copepods and fish, and they are capable of altering the composition of coastal planktonic communities. Here, we demonstrate that the predatory success of the lobate ctenophore Mnemiopsis leidyi lies in its use of cilia to generate a feeding current that continuously entrains large volumes of fluid, yet is virtually undetectable to its prey. This form of stealth predation enables M. leidyi to feed as …

Actions Of Ncx, Pmca And Serca On Short-Term Facilitation And Maintenance Of Transmission In Nerve Terminals, Mohati Desai-Shah, Robin L. Cooper

Biology Faculty Publications

Residual Ca²⁺ can accumulate in the nerve terminal during repetitive stimulation; thus, the basis for short-term facilitation (STF). The plasmalemmal Na⁺/Ca²⁺ exchanger [NCX], the Ca²⁺-ATPase (PMCA) and the sarcoplasmic/endoplasmic reticulum Ca²⁺-ATPase (SERCA) on the endoplasmic reticulum are three important Ca²⁺ regulatory processes in controlling [Ca²⁺]_i. The role of these [Ca²⁺]_i regulators in the development and maintenance of STF was addressed at the neuromuscular junction. When the NCX is compromised by reduced [Na+]o, the EPSP amplitudes decrease, but with KB-R7943 (a reverse blocker of NCX) …

Phenotypic Divergence During The Invasion Of Phyla Canescens In Australia And France: Evidence For Selection-Driven Evolution, Cheng-Yuan Xu, Mic H. Julien, Mohammad Fatemi, Christophe Girod, Rieks D. Van Klinken, Caroline L. Gross, Stephen J. Novak

Biology Faculty Publications and Presentations

Rapid adaptive evolution has been advocated as a mechanism that promotes invasion. Demonstrating adaptive evolution in invasive species requires rigorous analysis of phenotypic shifts driven by selection. Here, we document selection-driven evolution of Phyla canescens, an Argentine weed, in two invaded regions (Australia and France). Invasive populations possessed similar or higher diversity than native populations, and displayed mixed lineages from different sources, suggesting that genetic bottlenecks in both countries might have been alleviated by multiple introductions. Compared to native populations, Australian populations displayed more investment in sexual reproduction, whereas French populations possessed enhanced vegetative reproduction and growth. We partitioned …

Roles Of The Sarcoplasmic/Endoplasmic Reticulum Ca2+-Atpase, Plasma Membrane Ca2+-Atpase And Na+/Ca2+ Exchanger In Regulation Of Heart Rate In Larval Drosophila, Mohati Desai-Shah, A. R. Papoy, M. Ward, Robin L. Cooper

Biology Faculty Publications

We investigated the roles of three regulatory proteins that impact [Ca²⁺]_i within cardiac myocytes of Drosophila melanogaster. The NCX (Na⁺/Ca²⁺ exchanger), PMCA (plasma membrane Ca²⁺-ATPase) and SERCA (sarcoplasmic/endoplasmic reticulum Ca²⁺-ATPase) were compromised by ionic, pharmacological, mutationalmanipulation, and with a combination of approaches, while heart rate (HR) was monitored. A decrease in SERCA function reduced HR more for intact larva in comparison to a dissected larva. Dissected preparations were used to expose the heart directly to agents. A compromised PMCA also reduced HR; however, attenuated NCX function by low [Na^{+ …}

Ohio Conservation Plan: Plains Gartersnake, Thamnophis Radix, Norman Reichenbach, M. Barrie, K. Becka, G. Burghardt, S. Butterworth, C. Caldwell, F. Dierkes, P. Johantgen, K. Stanford, D. Wynn

Faculty Publications and Presentations

This plan outlines strategies and methods used in an ongoing study initiated in 1999 to restore a selfsustaining population of the Plains gartersnake (Thamnophis radix) in Ohio. Restoring a self-sustaining population would require increases in the current population to where the ratios of T. radix to T. sirtalis are approximately 1:1 in multiple locations in Killdeer Plains Wildlife Area (KPWA). This ratio would be similar to what was seen earlier by Reichenbach and Dalrymple (1986) at one site in KPWA.

The plan was developed by a team of enthusiastic conservationists representing, the Division of Wildlife (ODW), the Columbus and Cleveland …