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Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single ~52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity …

Investigation Of Biochemical Mechanisms Associated With Insulin Resistance In The Non-Insulin-Dependent Diabetes Mellitus, To-Yu Huang

Doctoral Dissertations

Non-insulin dependent diabetes mellitus (NIDDM) is a heterogeneous disease arising from metabolic defects in insulin secretion and insulin action. The inability of insulin to trigger full metabolic responses results in impaired glucose transport, which elicits compensatory hyperinsulinemia with increased lipogenesis. In order to identify the molecular defects responsible for insulin resistance, we have investigated three different model systems: the ability of sulfonylurea receptor (SUR) ligands to modify insulin regulated events in cultured rodent adipocytes; the ability of agouti and/or agouti related polypeptide (AGRP) to modify insulin regulated events; and the regulation of the activity and amount of the HNF-1a transcription …

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.

Analysis Of Events Governing The Meiotic Division In Mouse Spermatocytes, Shannon Stewart Eaker

Doctoral Dissertations

The meiotic division is essential for successful gametogenesis. However, many events occurring during male and female meiotic development remain poorly understood. While it is known that chromosomes must pair, recombine, and segregate to form gametes, critical questions remain. How and when do these events occur with respect to each other? What mechanisms monitor their developmental success? Insight into these questions is provided in this dissertation, using the mouse spermatocyte as a model. The purpose of this work is to aid in the overall understanding of mammalian meiosis.

After an introduction into mammalian meiosis in Part I, a temporal order of …

Protein Targeting And Translocation, Barry D. Bruce, D.A. Phoenix

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.

Some Aspects Of Lipid Metabolism Of Chicks Infected With Salomonella Pullorum, Charles Dean Jeffries

Doctoral Dissertations

The literature concerning the pathology accompanying infectious diseases has concerned, predominantly, gross and microscopic pathology. The biochemical changes in pathologic conditions have had little attention except for the generally accepted clinical procedures. Within the last decade, however, a change in emphasis has begun. A number on investigators have reported studies of enzymatic changes observed in infected animals. Many of these observations, however, have been with unnatural host-parasite systems.

The desirability of using a natural host-parasite system is obvious. Among the Enterobacteriaceae there are a limited number of complexes which are suitable for study with experimental animals. One of these is …