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Effects Of Essential Oils Alone Or In Combination With Monensin In An In Vitro Model Of Ruminal Acidosis, Jorge L. Bonilla Urbina

Electronic Theses and Dissertations

The objective of this study was to evaluate the effect of essential oils and/or monensin on the concentration of volatile fatty acids (VFA), ammonia, and pH in a ruminal acidosis in vitro model. Red Angus steers (n = 4; BW = 435 ± 9 kg) with ruminal and duodenal cannulas were allocated in a 4 × 4 Latin square design. Treatments were 1) Fed no essential oils (RB) or monensin sodium (MON:CON); 2) Fed RB at a rate of 14 g daily with no Mon (RB+); 3) Fed no RB and fed Mon at 400 mg daily (MON+); 4) Fed …

An Edna Assessment Of The Diamond Darter (Crystallaria Cincotta) In Its Current And Historic Distribution Range, Cameron Moore Layne

Graduate Theses, Dissertations, and Problem Reports (ETD)

Environmental DNA (eDNA) can serve as a useful monitoring tool for cryptic and imperiled freshwater stream fishes. eDNA can fill knowledge gaps regarding species’ distribution, site abundance, and habitat usage. The last known stronghold of the Diamond Darter (Crystallaria cincotta) in the Elk River of West Virginia provides an excellent test case to examine the utility of this non-invasive method of surveying rare species. Diamond Darters are one of the most newly recognized freshwater fishes in North America and are among the most imperiled U.S. fishes, having been federally listed as endangered in 2013. The cryptic behavior and limitations of …

Longitudinal And Ensemble Modeling Of Beef Cattle Feed And Water Intakes, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Beef cattle production remains a cornerstone of global food systems, providing highly bioavailable protein and essential micronutrients that are difficult to secure from plant-based foods alone. Yet this production is also resource-intensive, requiring substantial land, feed, and water inputs while contributing significantly to greenhouse gas emissions. Efficiency of dry matter intake (DMI) and water intake (WI) is central to both productivity and sustainability, but accurate measurement of these traits—particularly in grazing systems—remains a major limitation. National WI standards continue to rely on pen-level data collected in the 1950s, while most existing DMI prediction equations perform poorly when applied beyond the …

Tailwater Trout Population Dynamics, Connor Dylan Cunningham

Graduate Theses/Dissertations

Dams have been constructed throughout the world and provide several resources for humans (e.g., flood control, hydropower). These structures heavily alter the upstream and downstream environment. One consequence of these alterations is the creation of “tailwaters”, which can create cold-water habitat via hypolimnetic releases. In the southeast United States these tailwater environments are often managed for the recreational use of nonnative salmonids. The unnatural flow regimes of tailwaters affect the biotic and abiotic conditions and ultimately the dynamic rate functions (i.e., recruitment, growth, mortality) of the fish inhabiting them. As such, the goal of this research is to provide fisheries …

Leukocyte Infiltration Profiles At The Site Of Intradermal Injection Of Silikon-1000 In Scleroderma-Prone Ucd200/206 And Healthy White Leghorn Chickens, Katee L. Johnson, Jossie M. Santamaria, Chrysta N. Beck, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Intradermal injections of silicone-based fillers for the face and body may cause chronic inflammation and fibrotic reactions in some individuals. Chickens from the UCD 200/206 White Leghorn (WL) line spontaneously develop a fibrotic autoimmune disease known as scleroderma/systemic sclerosis (SSc). Using the SSc-prone UCD and healthy WL chickens, a longitudinal study was conducted to examine the cellular inflammatory responses to intradermal (i.d.) injections of sterile SILIKON-1000. Specifically, the pulp (a skin derivative) of growing feathers (GFs) was i.d. injected with sterile SILIKON-1000 or phosphate-buffered saline (PBS) as injection control (8 and 3 birds/line, respectively; 10 µL/GF; 20 GFs/bird). Two GFs …

Identification Of Salt Tolerance And Stress Response In Us Department Of Agriculture Tomato Germplasm At The Seedling Stage, Ibtisam Alatawi, Haizheng Xiong, Kenani Chiwina, Hanan Alkabkabi, Qun Luo, Neelendra K. Joshi, Xiaolun Sun, Xuan Zhuang, Kai-Shi Ling, Ainong Shi

Horticulture Faculty Publications and Presentations

Soil salinity is a significant abiotic factor that impedes sustainable crop production in key agricultural regions worldwide. Saline cultivation adversely affects soil quality, whereas the use of saline water for irrigation disrupts the physiological and biochemical processes of plants. Continuous irrigation with high salt concentrations leads to a gradual buildup of soil salinity, thus hindering optimal plant growth and development. Consequently, there is a growing emphasis on breeding salinity-tolerant cultivars of various crops. In this study, 71 tomato accessions sourced from 20 countries and provided by the US Department of Agriculture were evaluated under controlled greenhouse conditions and subjected to …

Spatiotemporal Variability In Invasive Carp Spawning Activity In Two Midwestern River Basins, Braden A. Whisler

Masters Theses

Across the United States, fisheries managers and agencies continue to battle the spread of invasive bigheaded carp (Hypophthalmichthys spp.). Although previous research has identified environmental parameters such as temperature and flow to be triggers of spawning activity, many of these studies focus on larger rivers, rarely focusing on the specific thresholds or timing of when a spawning event takes place. I utilized the differing hydrology of the Illinois and Wabash Rivers and their tributaries to examine the differences in environmental parameters needed to trigger bigheaded carp reproduction. Three mainstem sites and three tributaries of both basins were sampled …

Pharmacological Safety Profiling Of Nigella Sativa (Ns) And Hibiscus Sabdariffa (Hs) Extracts In Zebrafish (Danio Rerio) Embryos: An Integrative Vet-Pharmacotoxicological Evaluation Of Lc₅₀, Teratogenicity, And Developmental Outcomes, Ahmed E. A. Mostafa

Mansoura Veterinary Medical Journal

Herbal medicines are deeply rooted in Egyptian and Middle Eastern traditions, with Nigella sativa (NS) and Hibiscus sabdariffa (HS) being among the most widely consumed remedies. Despite extensive pharmacological evidence of their therapeutic benefits, systematic safety validation remains limited. Conventional toxicity assessments predominantly rely on rodents and higher vertebrates, which are costly, time-consuming, and ethically challenging. This gap necessitates alternative models that align with the 3R principles (Reduction, Replacement, Refinement). In this study, the zebrafish (Danio rerio) embryo model was employed to evaluate the developmental toxicity and median lethal concentration (LC50) of NS and HS aqueous extracts. Zebrafish embryos at …

Ocean Acidification Mitigation: Potential Expansion Of Sav Restoration Strategy In The Chesapeake Bay, Cree N. Taylor

Undergraduate Research Posters

Zostera marina is a species of marine macrophyte that has been largely prioritized in past submerged aquatic vegetation (SAV) restoration initiatives within the Chesapeake Bay because of its 1) previous abundance, 2) demonstrated carbon burial abilities, and 3) observed ability for meadows of Z. marina to ameliorate low pH conditions. Due to the temperature variability and poor water quality associated with anthropogenic disturbances in the Chesapeake Bay, Z. Marina beds have experienced several die-off events with limited capacity for regrowth. This systematic review explored the possible benefits of focusing future SAV recovery initiatives on ensuring a diverse variety of SAV …

Evaluation Of Blue Catfish Populations In Four Midwestern Power Cooling Reservoirs, Tyler Joseph Murray

Masters Theses

Blue Catfish (Ictalurus furcatus) are the largest catfish in North America and as such angler desire for trophy fisheries have grown in addition to harvest fisheries. The Illinois Department of Natural Resources (IDNR) has stocked Blue Catfish into several reservoirs in the state including four that have powerplants that utilize the water for cooling and discharge it back into the reservoir at increased temperatures. This study explored the potential impacts of these warm water effluents on Blue Catfish population demographics, as well as whether management changes need to be implemented in these systems. I sampled Powerton, LaSalle, Springfield, …

Prospective Use Of Detection Dogs As A Screening Tool For Mycoplasma Ovipneumoniae In Bighorn Sheep (Ovis Canadensis), Michele C. Lovara

Electronic Theses and Dissertations

Mycoplasma ovipneumoniae is a bacterium that can spill over from domestic sheep (Ovis aries) to bighorn sheep (Ovis canadensis), with effects in bighorn sheep ranging from low to no lamb recruitment to whole-herd all-age die-offs from resulting pneumonia. Fast and reliable pathogen or disease detection is essential for managing outbreaks in bighorn sheep and emergences in affected populations. Detection dogs are increasingly being enlisted to close gaps and further complement various disease, pathogen and virus research and response efforts. This thesis explores the use of conservation detection dogs as a potential tool for M. ovipneumoniae surveillance in bighorn sheep through …

Phytogenic Feed Additives Pre- And Post-Calving And Effects Of Substituting Ddgs With Soybean Meal On Protein Metabolism In Beef Cattle, Grace H. Jardon

Electronic Theses and Dissertations

The objective of these studies was to evaluate nutritional strategies to improve beef cattle productivity through targeted supplementation and protein source manipulation. The first study investigated the effects of a phytogenic supplement (PHYT; a blend of turmeric, capsicum, and black pepper oleoresin) on colostrum yield and composition, calf passive immunity, and cow–calf performance. Twenty-three Angus-based cows were assigned to either a control group or received 250 or 500 mg/head/day of PHYT from 30 days pre-calving to 60 days post-calving. Supplementation with PHYT linearly increased colostrum fat and calf serum IgA concentrations. Milk fat, calf serum IgG, and colostrum IgM tended …

Investigating The Ruminal Metagenome Of Grass And Forage-Fed Bison To Uncover Metabolic Activities That Impact The Efficiency Of Plant Fiber Utilization, Anlly Fresno Rueda

Electronic Theses and Dissertations

This dissertation investigates the ruminal microbiome of American plains bison (Bison bison) to uncover the metabolic activities that enable efficient utilization of plant fiber, with a particular focus on bacterial composition and functional capabilities in response to forage quality. Using both 16S rRNA gene sequencing and metagenomic approaches, this research provides a comprehensive view of microbial diversity and metabolic potential in the rumen of bison, offering insights into their ecological resilience and efficiency in nutrient extraction. Chapter 2 focuses on the metabolic potential of bacterial communities in the rumen of bison fed a grass-based diet. Using high-throughput sequencing and metagenomic …

Identification Of Gut Bacterial Symbionts From Atlantic Salmon That Are Involved In Metabolizing Feed Ingredients Derived From Soybean Meal, Emily Fowler

Electronic Theses and Dissertations

In the aquaculture industry, plant-based protein sources have emerged as an economically and environmentally sustainable replacement for traditional animal protein sources. Yet, due to reduced digestibility, the presence of anti-nutritional factors, as well as an increased risk of inflammation of the digestive system, the inclusion of plant-based protein ingredients must be limited, especially in the diets of carnivorous fishes. To gain further insight on how to adapt the fish digestive tract to more effectively utilize or tolerate plant-based protein ingredients, slurry from cultured Atlantic Salmon (Salmo salar) was used as a proxy in an in vitro model system to identify …

Synchronization Protocols Utilizing Progesterone Implants Associated With Estrus Expression, Vaginal Microbiome, And Immune Response In Beef Cattle, Riley James Puck

Electronic Theses and Dissertations

This thesis aims to provide a deep understanding of presynchronization strategies and the varying factors affecting their efficacy. Beginning with a literature review of the estrous cycle and the endocrinology driving it. Building on this foundation, the physiological basis for estrous synchronization is described, followed by the advancement of pre-synchronization protocols designed to increase estrus and conception. Further, we will focus on the influence of estrus expression on insemination time and conception rates. Finally, how the immune system interacts with the conception process. In addition, the review goes beyond exogenous endocrine manipulation and dives into the emerging role of the …

One Rung At A Time: Climbing The Ladder Toward Aquatic Connectivity In Eastern South Dakota, Evan Spencer

Electronic Theses and Dissertations

Roads and streams are synonymous with their connection to movement, the acquisition of resources, and as pathways to new opportunities. However, the factors that define good roads and ecologically connected streams are rarely without conflict. Road infrastructure at stream-road crossings has the potential to fragment stream networks. Tube culverts, where streams pass under the road through metal pipes, are particularly concerning for stream connectivity. Undersized, aging, or inappropriately installed culverts can develop vertical drops at the outflow due to high velocities and stream bed scouring. The outlet drop can function as a barrier, preventing the upstream movement of fish. Due …

Evaluating And Mapping Habitat Suitability And Effects Of Woodland Treatment On Breeding Pinyon Jays In Colorado, Emily Diane Macklin

Electronic Theses and Dissertations

The pinyon jay (Gymnorhinus cyanocephalus) is a highly social, colonially nesting corvid endemic to the interior western United States whose populations have declined by ~72% since 1966, prompting the species’ current review for listing under the Endangered Species Act. Pinyon jays inhabit pinyon pine (Pinus subsect. Cembroides) and juniper (Juniperus spp.) woodlands and other dry conifer woodlands, portions of which are treated through tree thinning and removal for fuels reduction, ecological restoration, and habitat improvement for wildlife. As a result there is pressing need to better understand how woodland treatment and other potential drivers impact pinyon jay habitat suitability and …

Spatiotemporal Habitat Use, Sex-Specific Movement, And Site Fidelity Of Walleye In Lake Sharpe, Sd, Carter S. Dollen

Electronic Theses and Dissertations

Walleye Sander vitreus are a popular sportfish among anglers in South Dakota with large economic impacts to local communities. A popular destination for Walleye anglers is Lake Sharpe, a 25,000-ha reservoir on the Missouri River, in central South Dakota. Gill net catches in Lake Sharpe show high annual variation, prompting concern that gill net catch rates may be poorly related to population abundance. A better understanding of Walleye movement and distribution could lead to increased precision in annual abundance estimates and improved active management that increases angler success. The goals of this study were: 1) determine spatiotemporal movement patterns of …

Extended Days On Feed: Influences On Growth Performance, Efficiency, And Carcass Characteristics Of Steers And Heifers Of Different Proportions Of Angus And Limousin Genetics, Grace Henley Olinger

Electronic Theses and Dissertations

Recently, cattle feeders have extended days on feed (DOF) resulting in hot carcass weights (HCW) exceeding the limits of existing serial slaughter datasets. This study's goal was to determine the impact extended DOF exerts on growth performance, feed efficiency, and carcass characteristics of steers and heifers with varying proportions of Angus (AN) and Limousin (LM) genetics. In 2022, commercial AN cows from two MT ranches were artificially inseminated to 9 sires from 3 breeds (AN, LM, and Limflex (LF)) with parentage confirmed by DNA. In 2023, 216 calves (sired by 72 AN, 73 LF, and 71 LM; 105 steers and …

Binoculars To Bytes: Development And Field Validation Of An Ai-Driven System For Avian Monitoring, Christian J. Dupree

Graduate Student Theses, Dissertations, & Professional Papers

Autonomous camera-trap arrays coupled with artificial-intelligence (AI) vision can lift bird monitoring beyond the spatial, temporal, and labor limits of traditional field surveys. We present Binoculars to Bytes (B2B), an open-source pipeline that turns 180–360° time-lapse imagery into analysis-ready avian data. At Freezout Lake Wildlife Management Area (Montana, USA) the system ran four-hour morning deployments during spring and fall migrations (2023–2024). A YOLO-NAS detector, incrementally refined with a “Specialized Localized Iterative Model” workflow, quadrupled local accuracy and, after confidence-based species-binning, cut false-positive rates in half. Daily AI species lists were benchmarked against contemporaneous eBird citizen-science checklists and recovered ≥ …

Exploring The Ruminal Microbiome And Its Impacts On Feed Efficiency In Beef Cattle, Taylor Simone Sidney

Graduate Theses, Dissertations, and Problem Reports (ETD)

The ability for beef cattle to utilize feed efficiently is a critical determinant for both economic and environmental sustainability in livestock production. However, despite extensive research, the complex microbial ecosystem within the rumen and its relationship to feed efficiency remain incompletely understood. This dissertation explores the physiological and microbial foundations of feed efficiency by characterizing how rumen microbial communities and their functions differ among beef cattle with divergent residual feed intake (RFI) phenotypes and how the RFI status of beef cattle influenced their response to dietary interventions such as supplementation with a Bacillus-based feed additive. In the first study, …

The Prize Biter Of Modernity? Investigating Killer Whale Bite Mechanics, Daniel Hong

West Chester University Graduate Theses, Dissertations, and Final Projects

Although the killer whale (Orcinus orca) is a cosmopolitan apex predator, very little work has been done on its feeding biomechanics. Here I use finite element analysis to investigate the mandibular stiffness of a juvenile male Bigg’s killer whale, and compare it to that of the false killer whale (Pseudorca crassidens) and the saltwater crocodile (Crocodylus porosus). Full mandibles and left and right halves of mandibles are simulated under FEA. I also estimate the total jaw muscle force produced by the Bigg’s killer whale using the dry skull method. The killer whale lacks an …

Exploring Coastal Plain Fish Ecology Across A Range Of Early-Life-History Traits And River Types, Bridgette Nicolosi

College of Graduate Studies: Theses & Dissertations

The ecological complexity of southeastern Coastal Plain rivers is reflected in the diversity of fish communities they support, yet the processes structuring early life dynamics remain poorly understood. This thesis explores the influence of environmental gradients on larval fish assemblage composition and American Shad (Alosa sapidissima) spawning phenology across two Georgia Coastal Plain river systems.

In the unregulated Ogeechee River, I investigated spatial and temporal variation in larval fish communities by comparing mainstem and tributary habitats to understand the role of tributaries in watershed-wide larval production. Larvae were collected biweekly from May to September 2024 at eight paired mainstem-tributary sites …

Biological Control Of Ticks In Domestic Environments: Modeling The Potential Impact Of Entomopathogenic Fungi On The Transmission Of East Coast Fever In Cattle, Joseph Wang Ang A. Oundo, Nienke Hartemink, Mart C.M. De Jong, Constantianus J.M. Koenraadt, Shewit Kalayou, Daniel Masiga, Quirine Ten Bosch

All Peer-Reviewed Publications

Biological control of ticks using entomopathogenic fungi (EPF) is a highly desired alternative to chemical acaricides for the control of tick-borne pathogens. For Metarhizium anisopliae isolate ICIPE 7, one of these EPFs, efficacy against multiple tick species has been demonstrated in laboratory and field settings. However, we currently have little quantitative understanding of how EPFs can impact transmission. We developed a deterministic model of tick–host–pathogen interactions to explore how the effects of EPF on Rhipicephalus appendiculatus ticks may impact the transmission dynamics of East Coast fever (ECF) in cattle populations. We parameterized the multi-faceted effects of EPFs on tick dynamics …

Stress And Behavior In Beef Calves During Weaning, Kathleen E. Tierney

Theses and Dissertations

Animal welfare in commercial livestock operations has been an increasing topic of concern. It is well known that stress can have significant behavioral and physiological repercussions. It is especially important in commercial operations to reduce stress, as animal efficiency is the leading priority. In an effort to reduce stress on animals and improve welfare in livestock operations, several studies have evaluated common husbandry practices. One of the most widely studied practices is weaning, which encapsulates the process of removing the young from the dam. Several studies have evaluated the effects of weaning on beef cattle through a variety of behavioral …

Urban Coyotes As A Source Of Conflict With Humans: An Evaluation Of Common Management Practices, Paul D. Curtis, David L. Bergman, Angeline Barker, Daniel Bogan, Mary Ann Bonnell, Pierre Canac-Marquis, David Drake, Dave Kay, Brian F. Wakeling, H. Bryant White

Human–Wildlife Interactions Monographs

Wildlife often serves as a catalyst for conflict among stakeholders. Management actions that are intended to address sources of disagreement may initiate additional conflict. The Association of Fish and Wildlife Agencies Human–Wildlife Conflict Working Group initiated an effort to identify management actions commonly used to address unwanted coyote (Canis latrans) interactions within urban environments and identify the benefits and challenges associated with each action. Challenges with coyotes in urban environments include aggressive behavior toward humans and pets, attacks, disease exposure, and domestic livestock damage. Although elimination of all attractants would prove preventative in most instances, in practice this …

Effects Of A Diet Supplemented With A Blend Of Protected Organic Acids And Essential Oilson Growth Performance And Cecal Microbiota Composition In Broilers, Mehmet Akan, Kadi̇r Serdar Di̇ker, Serdar Özlü, Murat Çelebi̇, Seyyi̇de Sariçam İnce, Barişhan Doğan, Okan Eli̇bol

Turkish Journal of Veterinary & Animal Sciences

Organic acids (OAs) and essential oils (EOs) have several positive effects on the gut microbiota of broilers. In this study, weaimed to determine the efficacy of a blend of protected OAs and EOs [P(OA+EO)] on the growth performance and cecal microbiota ofbroiler chickens. A total of 160 Ross 308 broiler chickens, including 80 male and 80 female birds, all 1 day old, were randomly distributedbetween the control group (basal diet) and the treatment group [basal diet + 300 g/t P(OA+EO)] according to an 8-pen arrangementwith 4 replicates of 20 birds. Body weight (BW), body weight gain (BWG), feed consumption (FC), …

Précis Of The Edge Of Sentience: Risk And Precaution In Humans, Other Animals, And Ai, Jonathan Birch

Animal Sentience

We often face grave practical decisions that seem to hinge on whether a system is sentient. This family of cases includes invertebrate animals, people who are unresponsive after brain injury, fetuses, neural organoids, and now AI technologies. We must decide what to do despite ongoing disagreement about the nature of sentience. In our state of uncertainty, we should pragmatically transform the question from “Is it sentient?” to “Is it a sentience candidate, an investigation priority, or neither?”. When a system is a sentience candidate, it is negligent to fail to consider precautions. We should instead evaluate precautions for their proportionality …

Food Finding Ability Does Not Predict Annual Survival In Winter Resident Birds, Aidan Hand

Honors Program: Senior Projects (Public)

Small non-migratory birds in temperate locations face many challenges to survive the winter. They must be able to find enough food, which is in decreased supply, to fuel the energetic demands of staying warm. Prior work has demonstrated that birds show individual variation in their foraging behaviors that could contribute to the discovery of new foraging patches, such as novel environment exploration. A better ability to discover new food sources could increase a bird’s chance of surviving the winter by increasing its access to new food patches. To test this hypothesis, we used radio frequency identification (RFID)-enabled bird feeders to …

Addressing The Productivity-Susceptibility Analysis In Data-Limited Fisheries For Understudied Fish In The Marine Aquarium Trade, Alice Wynn, Jemelyn Baldisimo, Gabrielle Baillargeon, Michael Tlusty, Andrew Rhyne

Biological Sciences Faculty Publications

The marine aquarium trade (MAT) harvests millions of coral reef fishes from the wild yearly. While the MAT supports fishers, hobbyists and marine education, harvested fishes are crucial in maintaining coral reef health and balanced marine cycles within their native habitats. Understanding the impact of the MAT on fish populations is critical for sustaining the trade in the long-term. Productivity-susceptibility analyses (PSAs) offer semiquantitative methods of analyzing the productive capacity of each fish stock, as well as its susceptibility to overfishing. The present study revises and expands upon a PSA conducted by Baillargeon et al. (2020) that focused on 32 …