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Transcriptome Sequencing Analysis Of Ovarian Granulosa Cells In Fecb Hybrid Ewe Lamb, Cuiling Wu, Chunxin Wang, Bo Zhai, Yunhui Zhao, Zhuo Zhao, Yuezhen Tian, Kechuan Tian, Mingxin Zhang Jan 2020

Transcriptome Sequencing Analysis Of Ovarian Granulosa Cells In Fecb Hybrid Ewe Lamb, Cuiling Wu, Chunxin Wang, Bo Zhai, Yunhui Zhao, Zhuo Zhao, Yuezhen Tian, Kechuan Tian, Mingxin Zhang

Turkish Journal of Veterinary & Animal Sciences

The reproductive trait of livestock is part of the most important economic traits in animal husbandry. FecB is a major gene for multiplet traits in sheep. However, studies on FecB in lamb are not clear and comprehensive. The 1-month ewe lamb of F1 generation cross between Booroola Merino sheep and Xinji fine wool sheep was superovulated. Transcriptome sequencing was performed on ovarian granulosa cells of the FecB gene wild homozygous type (++ genotype) and FecB gene mutant heterozygous type (B+ genotype). We found 306 DEGs in the ovarian granulosa cells of the two genotype (170 genes more highly in B+ …

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Investigation Of Propolis In Terms Of Hygienic Quality, Some Pathogenic Bacteria And Nosema Spp., Hayretti̇n Akkaya, Gülay Merve Bayrakal, Emek Dümen Jan 2020

Investigation Of Propolis In Terms Of Hygienic Quality, Some Pathogenic Bacteria And Nosema Spp., Hayretti̇n Akkaya, Gülay Merve Bayrakal, Emek Dümen

Turkish Journal of Veterinary & Animal Sciences

Propolis collected from plants by honeybees is used for the construction and protection of hives. In addition, propolis has been used in the treatment of many diseases since ancient times because of its antimicrobial, antiseptic, antiinflammatory and antioxidant properties. Despite all these positive health effects, propolis can be microbiologically risky for many reasons such as environmental contamination and insufficient personnel hygiene. Therefore, chemical, physical, antimicrobial properties as well as microbiological properties are among the parameters to be investigated. This study aims to explore propolis?s initial bacteriological and parasitological flora using 5 different parameters (total coliform group, Escherichia coli, Staphylococcus aureus, …

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Possible Ensiling Of Pumpkin (Cucurbita Pepo) Residues, İsmai̇l Ülger, Mahmut Kali̇ber, Selma Büyükkiliç Beyzi̇, Yusuf Konca Jan 2020

Possible Ensiling Of Pumpkin (Cucurbita Pepo) Residues, İsmai̇l Ülger, Mahmut Kali̇ber, Selma Büyükkiliç Beyzi̇, Yusuf Konca

Turkish Journal of Veterinary & Animal Sciences

The aim of the current study was to determine the feasibility of ensiling pumpkin residues alone and with forage. For this purpose, chemical composition, nutritive values, and in vitro gas production of pumpkin residue silages were investigated. Pumpkin residue (PR), maize fodder (M), sugar beet pulp (SBP), and pomegranate pulp (PP) were ensiled alone, and pumpkin residues were ensiled with maize (PRM), sugar beet pulp (PRSB), and pomegranate pulp (PRP) in 50% (50:50 w:w) of each, as well as with 5% wheat straw (PRS) and alfalfa hay (PRA). After 2 months of storage, chemical compositions and Fleig scores of the …

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Genetic Polymorphism Of Alpha S1-Casein In Bulgarian Sheep Breeds And Its Effect On Milk Composition, Deyana Gencheva, Petya Veleva, Nikolina Naidenova, Darina Pamukova Jan 2020

Genetic Polymorphism Of Alpha S1-Casein In Bulgarian Sheep Breeds And Its Effect On Milk Composition, Deyana Gencheva, Petya Veleva, Nikolina Naidenova, Darina Pamukova

Turkish Journal of Veterinary & Animal Sciences

The genetic polymorphism of the alpha S1-casein (CSN1S1) was investigated in five sheep breeds reared in Bulgaria: Sofia (Elin-Pelin, SEPL), Copper-Red Shumen (CRSH), Local Karnobat (LKNB), Pleven Blackhead (PLBH), and Stara Zagora (STZG) sheep with an aim to establish the possible effect of a particular genotype on ovine milk composition. Based on nucleotide variation in exon III of the CSN1S1gene, two genetic variants (A and C) and three genotypes (AA, AC, and CC) have been identified using PCR-RFLP analysis on a total of 217 unrelated ewes. The allele frequencies determined a prevalence of the allele C (0.886) over the allele …

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Effects Of Sugar Beet Pulp Based Total Mixed Ration On Growth Performance And Blood Profile Status In Male Nili Ravi Buffalo Calves, Saeed Ahmed, Faisal Shahzad, Hifz-Ul- Rahman, Muhammad Rajab Jan 2020

Effects Of Sugar Beet Pulp Based Total Mixed Ration On Growth Performance And Blood Profile Status In Male Nili Ravi Buffalo Calves, Saeed Ahmed, Faisal Shahzad, Hifz-Ul- Rahman, Muhammad Rajab

Turkish Journal of Veterinary & Animal Sciences

Sugar beet pulp is comprised of highly digestible fibre that improves the growth rate in calves. The research was conducted to explore the effects of varying levels of sugar beet pulp (SBP) based total mixed ration (TMR) on growth performance and blood metabolites in buffalo male calves. Three TMRs were formulated based on quantity of SBP added i.e. 0% (TMR1), 15% (TMR2), 30% (TMR3) and assigned to 3 different groups (n = 6) of calves for a period of 60 days under a completely randomized statistical design. Calves were fed iso-nitrogenous and iso-caloric ration as 3% of BW/day on dry …

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Assessment Of Polymorphism On Kappa-Casein Gene Of Anatolian Water Buffalo Breedusing The Pcr-Rflp Method, Yusuf Özşensoy Jan 2020

Assessment Of Polymorphism On Kappa-Casein Gene Of Anatolian Water Buffalo Breedusing The Pcr-Rflp Method, Yusuf Özşensoy

Turkish Journal of Veterinary & Animal Sciences

Water buffalo breeding is practiced in various provinces of Turkey, and is also included in the National Breeding Project. It was aimed in this study to investigate polymorphisms on exon 4?intron 5 and exon 4 only of the kappa-casein (CSN3) gene for milk yields of water buffalo bred in Sivas Province. Blood samples were taken from 135 water buffaloes. The phenol/chloroform method for DNA extraction was conducted on collected blood. Polymerase chain reaction (PCR) was used to amplify CSN3-specific regions, and amplified products were run using 2% agarose gel electrophoresis (AGE). Various restriction endonucleases (RE) were utilized in respect to …

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An Atypical Localization Of Bacillus Anthracis In A Cow's Milk And Udder, Mi̇tat Şahi̇n, Fati̇h Büyük, Özgür Çelebi̇, Mustafa Reha Coşkun Jan 2020

An Atypical Localization Of Bacillus Anthracis In A Cow's Milk And Udder, Mi̇tat Şahi̇n, Fati̇h Büyük, Özgür Çelebi̇, Mustafa Reha Coşkun

Turkish Journal of Veterinary & Animal Sciences

A 6-year-old Simental-Swiss Brown cross-bred cow, which aborted in the first trimester of the gestation and involuntarily presented anthrax bacilli around the udder, was subjected to this study. The cow was vaccinated with an attenuated anthrax vaccine (Bacillus anthracis 34 F2) 6 months ago and had normal physical examination findings. Samples taken 10 days after the abortion were representative of all possible spaces that could carry the bacteria such as milk, blood, environment, and swabs from rectum, udder and tits of an infected cow. Microbiological examination was carried out by staining the smears with Azure and in vitro culture of …

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Determination Of Nutritional Value Of Some Quinoa Varieties, Tugay Ayaşan Jan 2020

Determination Of Nutritional Value Of Some Quinoa Varieties, Tugay Ayaşan

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the nutritive value of 5 quinoa varieties (Inia431- Altiplano, White, Titicaca, Illpa Inia, and Carmen). As a result of the study, it was found that there were statistically significant differences between the quinoa varieties in terms of dry matter (DM), crude ash (CA), crude protein (CP), crude fat (CF), neutral-detergent fiber (NDF), acid-detergent fiber (ADF), crude cellulose (CC), hemicellulose (HC), total digestible nutrients (TDN), digestible energy (DE), metabolizable energy (ME), and net energy lactation (NEL) (P < 0.05). This is a preliminary study. In vivo and vitro studies are required.

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Structural And Functional Changes In Corpus Luteum Of Single- And/Or Double-Ovulatedpregnant And Nonpregnant Ewes During The Spring And Autumn Seasons, Javad Habibizad, Hamed Karami, Mustafa Dolatabadi, Gaffari̇ Türk Jan 2020

Structural And Functional Changes In Corpus Luteum Of Single- And/Or Double-Ovulatedpregnant And Nonpregnant Ewes During The Spring And Autumn Seasons, Javad Habibizad, Hamed Karami, Mustafa Dolatabadi, Gaffari̇ Türk

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effect of ovulation numbers on the progesterone and luteal volume in nonpregnant and pregnant Lori-Bakhtiari ewes. In the spring season 48 reproductive tracts of nonpregnant ewes (treatments included single ovulation, unilateral double ovulations, and bilateral double ovulations) and in the autumn season 144 reproductive tracts of nonpregnant and pregnant ewes (single ovulation, unilateral double ovulations, bilateral double ovulations, single ovulation with 1 foetus, single ovulation with 2 foetuses, unilateral double ovulations with 1 foetus, unilateral double ovulations with 2 foetuses, bilateral double ovulations with 1 foetus, bilateral double ovulations with 2 foetuses) were …

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Functional And Whole Regression-Based Genome-Wide Association Analyses For Weightmeasurements Of Chicken Eggs, Burak Karacaören Jan 2020

Functional And Whole Regression-Based Genome-Wide Association Analyses For Weightmeasurements Of Chicken Eggs, Burak Karacaören

Turkish Journal of Veterinary & Animal Sciences

In genomic studies, complex traits can be modelled using repeated measures, thereby gaining a better understanding of the underlying biology. An increased number of measurements per individual might reduce measurement noise, thus increasing the likelihood of detecting true genomic signals. Here we aimed to predict genomic signals over a logistic curve referring to multiple underlying genetic architectures, for both simulated and longitudinal egg weight datasets. The chicken data were obtained from 92 sires and 801 dams of an 11th generation pure line, resulting in data from 1078 hens. We analysed longitudinal measurements of egg weights with 294,705 single nucleotide polymorphisms …

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The Determination Of Antimicrobial And Antibiofilm Activities Of Foodborne Lactic Acid Bacteria Against Enterobacter Cloacae Isolates, Beytullah Kenar, Mi̇ne Eri̇k, Sevi̇m Feyza Erdoğmuş, Safi̇ye Eli̇f Korcan, Zahi̇de Köse, Gül Durmaz Jan 2020

The Determination Of Antimicrobial And Antibiofilm Activities Of Foodborne Lactic Acid Bacteria Against Enterobacter Cloacae Isolates, Beytullah Kenar, Mi̇ne Eri̇k, Sevi̇m Feyza Erdoğmuş, Safi̇ye Eli̇f Korcan, Zahi̇de Köse, Gül Durmaz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the antimicrobial and antibiofilm activities of 10 different lactic acid bacteria (LAB) strains isolated from local food sources of animal origin against 4 Enterobacter cloacae isolates obtained from clinical cases and determine their adhesion potentials to intestinal epithelial cells. In this study, all Enterobacter cloacae isolates (P3, P4, P5, P7) identified with the BD Phoenix automation system were detected to form biofilm with both Congo red agar and Microtiter plate methods. Amoxicillinclavulanate, cefuroxime, and ampicillin resistance was determined in all isolates. It was determined that LAB strains producing exopolysaccharide (EPS) were able …

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In Vivo Biocompatibility And Fracture Healing Of Hydroxyapatite-Hexagonal Boron Nitridechitosan-Collagen Biocomposite Coating In Rats, Mehmet Zeki̇ Yilmaz Deveci̇, Ramazan Gönenci̇, İbrahi̇m Canpolat, Özgür Kanat Jan 2020

In Vivo Biocompatibility And Fracture Healing Of Hydroxyapatite-Hexagonal Boron Nitridechitosan-Collagen Biocomposite Coating In Rats, Mehmet Zeki̇ Yilmaz Deveci̇, Ramazan Gönenci̇, İbrahi̇m Canpolat, Özgür Kanat

Turkish Journal of Veterinary & Animal Sciences

The biocompatibility of orthopaedic implants and their effects on fracture healing have key roles for success. In this study, it was aimed to investigate the effects of a novel biocomposite consisting of hydroxyapatite (HA), hexagonal boron nitride (h-BN), chitosan (Cs), and type 1 collagen (Ct1) on biocompatibility and fracture healing in rats. A total of 60 adult male Wistar rats weighing 300?500 g were used in the study. The rats were randomly divided into 2 groups named A (uncoated/control) and B (biocomposite coated). Biocomposite (HA/h-BN/Cs/Ct1) coated and uncoated stainless-steel implants were used as intramedullary pins. Groups A and B were …

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Genotypic Diversity Of Salmonella Ser. Gallinarum Strains Isolated From 2012 To 2016 In Brazil, Diogo Luiz Gadotti, Priscila Belleza Maciel, Raquel Rebelatto, Sabrina Castilho Duarte, Diogenes Dezen Jan 2020

Genotypic Diversity Of Salmonella Ser. Gallinarum Strains Isolated From 2012 To 2016 In Brazil, Diogo Luiz Gadotti, Priscila Belleza Maciel, Raquel Rebelatto, Sabrina Castilho Duarte, Diogenes Dezen

Turkish Journal of Veterinary & Animal Sciences

Salmonella Gallinarum biovar Gallinarum (SG) is a host-specific avian pathogen causing fowl typhoid (FT), a notifiable disease that causes septicemia in poultry and significant economic losses. In the state of Santa Catarina, Brazil, several cases of FT were reported from 2012 to 2016. However, strains of these outbreaks have not yet been characterized, and the genotypic characterization of isolates is essential for epidemiological surveillance and outbreak investigations. The objective of this work was to study the genetic diversity of SG isolated from the FT outbreaks, aiming to identify similarity or dissimilarity among strains isolated from the reported foci. For this …

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Pistacia Atlantica Gum Aqueous Extract Modulates Humoral And Cellular Immuneresponses In Balb/C Mice, Hadi Esmeili Gouvarchin Ghaleh, Zaynab Shakarami Gandabeh, Bahman Mansouri Motlagh, Ali Sheikhian Jan 2020

Pistacia Atlantica Gum Aqueous Extract Modulates Humoral And Cellular Immuneresponses In Balb/C Mice, Hadi Esmeili Gouvarchin Ghaleh, Zaynab Shakarami Gandabeh, Bahman Mansouri Motlagh, Ali Sheikhian

Turkish Journal of Veterinary & Animal Sciences

Natural herbal products contain different compounds that can modulate immune and inflammatory diseases. This investigation aimed to examine the influence of Pistacia atlantica aqueous extract on cellular and humoral immunity in mice following the exposure to sheep red blood cells (SRBCs). For this purpose, male BALB/c mice (n = 40) were randomly assigned into 4 equivalent groups. The animals received 1 × 109 SRBCs in complete Freund?s adjuvant (CFA) intraperitoneally 3 times with two-week intervals. Five days after the final injection, blood samples were taken from the animals. Additionally, 1 × 109 SRBCs were also injected into the mice?s left …

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Antibody Detection Against Akabane (Aka) And Bluetongue (Bt) Viruses In Algeriandromedary Camels, Radhwane Saidi, Firat Doğan, Veysel Soydal Ataseven, Yaşar Ergün Jan 2020

Antibody Detection Against Akabane (Aka) And Bluetongue (Bt) Viruses In Algeriandromedary Camels, Radhwane Saidi, Firat Doğan, Veysel Soydal Ataseven, Yaşar Ergün

Turkish Journal of Veterinary & Animal Sciences

In this study, blood samples collected from dromedary camels in the Algerian provinces of Laghouat and Ghardaia, which were kept in the same pastures and in the same herds with sheep and goats, were examined to detect antibodies to the Bluetongue (BT) and Akabane (AKA) viruses using enzyme linked immunosorbent assay and serum neutralization tests, respectively. Overall, 16 (14.4%) and 2 (1.8%) of 111 tested camel serum samples were seropositive for the BT virus (BTV) and the AKA virus (AKAV), respectively. The present study determined that BTV infection was prevalent in camels in Algeria, and antibodies to the AKAV were …

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The Clinical Outcome Of Three Procedures For Extraarticular Stabilization Of Cranial Cruciate Ligament Injuries In Dogs, Cornel Igna, Roxana Dascalu, Daniel Bumb, Bogdan Sicoe, Cristian Zaha, Larisa Schuszler Jan 2020

The Clinical Outcome Of Three Procedures For Extraarticular Stabilization Of Cranial Cruciate Ligament Injuries In Dogs, Cornel Igna, Roxana Dascalu, Daniel Bumb, Bogdan Sicoe, Cristian Zaha, Larisa Schuszler

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the long-term outcomes in dogs with cranial cruciate ligament injury in which the stifle was stabilized with lateral suture stabilization, lateral suture stabilization-modified, or Securos lateral bone anchor tibial suture and monofilament nylon sutures placed at quasi-isometric points of the stifle. It is a retrospective study comprising a sample population of 49 client-owned dogs. Medical records from 2014?2016 were reviewed to identify dogs that had a cranial cruciate ligament rupture in which the stifle was stabilized using one of the aforementioned methods. The overall complication rate was 16.3% and the documented complication …

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Effect Of Sesamin On Protection Of Equine Articular Cartilage Degradation In Vitro, Siriporn Peansukmanee, Siriwan Tangyuenyong, Siriwan Ongchai Jan 2020

Effect Of Sesamin On Protection Of Equine Articular Cartilage Degradation In Vitro, Siriporn Peansukmanee, Siriwan Tangyuenyong, Siriwan Ongchai

Turkish Journal of Veterinary & Animal Sciences

Black sesame seed is a potential herbal medicine for osteoarthritis treatment as it contains sesamin, which has antiinflammatory properties and has demonstrated chondroprotective effects in many species. Horses are particularly susceptible to osteoarthritis. Inflammatory mediators and matrix metalloproteinase are important factors causing cartilage degradation. This study aimed to investigate the protective potential of sesamin (0.5-2.0 μM) against interleukin (IL)-1ß-induced equine cartilage explant degradation. The cartilage degradation was monitored by measuring the release of cartilage matrix molecules in culture media including sulfatedglycosaminoglycans and hyaluronan by calorimetric assay and ELISA assay, respectively. The remaining contents of collagen and uronic acid (UA) within …

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Identification Of Microbiological, Physical, And Chemical Quality Of Milk From Milkcollection Centers In Kastamonu Province, Di̇lek Özdemi̇r, Deren Tahmas Kahyaoğlu Jan 2020

Identification Of Microbiological, Physical, And Chemical Quality Of Milk From Milkcollection Centers In Kastamonu Province, Di̇lek Özdemi̇r, Deren Tahmas Kahyaoğlu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study is to perform microbiological, physical, and chemical analyses of the samples from producers who continuously bring milk to 30 actively operating milk collection centers throughout the spring, summer, autumn, and winter seasons in Kastamonu Province and determine whether the identified values are within legal limits. For microbiological properties of milk included in the research, total aerobic mesophilic bacteria (TAMB) and coliform group bacteria counts were identified. For the determination of physical and chemical properties, dry matter, fat, nonfat dry matter, lactose, protein, ash, pH, titratable acidity, refractive index, and specific gravity values were identified and …

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Embryonic Mortality In Sheep: A Review, Pavitra Chundekkad, Barbara Blaszczyk, Tomasz Stankiewicz Jan 2020

Embryonic Mortality In Sheep: A Review, Pavitra Chundekkad, Barbara Blaszczyk, Tomasz Stankiewicz

Turkish Journal of Veterinary & Animal Sciences

Despite extensive research on the reproductive health of domestic animals, embryonic mortality continues to be a problem that brings about significant losses for both commercial breeding and scientific research. This review paper discusses the genetic and environmental factors that affect embryo mortality rates in sheep along with the different techniques developed to overcome this issue. Additionally, one of the objectives of this study is to emphasize the importance of the use of time-lapse cinematography and color Doppler ultrasonography as quick and reliable methods for early detection of pregnancy and embryonic death.

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Ginkgo Biloba Extract Egb761 Improved Anti-Heat Stress Responses In Chickens In Vivo Via Regulation Of Heat-Shock Protein Expression And Distribution, Xiaohui Zhang, Miao Zhang, Endong Bao Jan 2020

Ginkgo Biloba Extract Egb761 Improved Anti-Heat Stress Responses In Chickens In Vivo Via Regulation Of Heat-Shock Protein Expression And Distribution, Xiaohui Zhang, Miao Zhang, Endong Bao

Turkish Journal of Veterinary & Animal Sciences

Heat stress is a lethal threat to chickens. This study investigated the protective effect of a Ginkgo biloba extract EGB761 against heat stress in chickens in vivo. A total of 200 one-day-old hens were separated randomly into control (Con), heat stress (HS), 0.1% EGB administration and heat stress (0.1% EGB+HS), 0.3% EGB administration and heat stress (0.3% EGB+HS), and 0.6% EGB administration and heat stress (0.6% EGB+HS) groups. After EGB761 administration for 45 days, ten chickens were selected randomly, assigned to one of the designated groups, and exposed to a one-time heat stress condition of 38 ± 1 °C for …

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The Forage Quality And The In Vitro Ruminal Digestibility, Gas Production, Organic Acids, And Some Estimated Digestion Parameters Of Tomato Herbage Silage With Molasses And Barley, Mahmut Teki̇n, Kanber Kara Jan 2020

The Forage Quality And The In Vitro Ruminal Digestibility, Gas Production, Organic Acids, And Some Estimated Digestion Parameters Of Tomato Herbage Silage With Molasses And Barley, Mahmut Teki̇n, Kanber Kara

Turkish Journal of Veterinary & Animal Sciences

In this study, nutrient composition and use of silage after harvest of waste green parts (herbage) of tomato ( Licopersicon esculentum L.) were researched as a wet forage source for ruminants. Three different silages of tomato herbage: without additive (TS), with 5% sugar beet molasses (TSm), or 5% crushed barley grain (TSb) were prepared. The forage quality and the in vitro ruminal digestibility, gas production, organic acids, and some estimated digestion parameters for different silages of tomato herbage were determined. Besides, the in vitro digestion values of using at 25%, 50%, 75%, and 100% rates instead of corn silage in …

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Determination Of Some Coagulation Parameters According To Age And Sex In Sivas Kangal Dogs, Burcu İnsal, İlksi̇n Pi̇şki̇n Jan 2020

Determination Of Some Coagulation Parameters According To Age And Sex In Sivas Kangal Dogs, Burcu İnsal, İlksi̇n Pi̇şki̇n

Turkish Journal of Veterinary & Animal Sciences

Hemostasis is stopping bleeding in a controlled manner whereas coagulation is a series criterion and to diagnose disorders caused by coagulation in an early period is to determine the coagulation time. These phases are controlled with various tests. In this study, the aim is to determine some coagulation parameters in Sivas Kangal dogs by taking the age and sex into consideration. Coagulation is vitally important in many physiological and pathological cases. The most important tests, complete blood count with peripheral blood smear, prothrombin time (PT), activated partial thromboplastin time (aPTT), and D-dimer fibrin degradation product assay were applied to all …

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Dmem And Fbs As Thawing Solutions For Frozen Semen Of Pigs Can Improve Sperm Motility And Sow Reproductive Performance, Peng Zheng, Ziming Wang, Xiaoyu Li, Fushuo Huang, Mingjun Ma, He Huang Jan 2020

Dmem And Fbs As Thawing Solutions For Frozen Semen Of Pigs Can Improve Sperm Motility And Sow Reproductive Performance, Peng Zheng, Ziming Wang, Xiaoyu Li, Fushuo Huang, Mingjun Ma, He Huang

Turkish Journal of Veterinary & Animal Sciences

The purpose of this experiment was to investigate the effects of Beltsville thawing solution (BTS), Androhep, and Dulbecco?s modified Eagle medium + Fatal bovine serum (D-F) as diluents of frozen semen on sperm motility and sow reproductive performance. In experiment 1, boar semen was collected and diluted to 2.0 × 109/60 mL, 1.5 × 109/60 mL, and 1.5 × 109/40 mL with Androhep. The appropriate semen dose was determined by postcervical insemination. In experiment 2, boar semen was fumigated with liquid nitrogen and thawed semen was diluted to 1.5 × 109/40 mL with D-F, Androhep, and BTS, respectively. The effects …

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Expression Profiles Of Lincrna And Mrna Related To Milk Yield And Milk Composition Traits In The Milk-Derived Exosomes Of Holstein And Doğu Anadolu Kırmızısı Cows, Selçuk Özdemi̇r, Ömer Eltas, Muhammed Hüdai̇ Çulha Jan 2020

Expression Profiles Of Lincrna And Mrna Related To Milk Yield And Milk Composition Traits In The Milk-Derived Exosomes Of Holstein And Doğu Anadolu Kırmızısı Cows, Selçuk Özdemi̇r, Ömer Eltas, Muhammed Hüdai̇ Çulha

Turkish Journal of Veterinary & Animal Sciences

This study aimed to demonstrate the expression profiles of lincRNAs and mRNAs affecting milk yield and composition traits in the milk-derived exosomes of Holstein and Doğu Anadolu Kırmızısı (DAK) cows. For this purpose, the locations of these specific lincRNAs and mRNAs were confirmed in quantitative trait loci. Then RT-PCR analysis was performed to identify the expression profiles of the lincRNAs and mRNAs. Lastly, correlation analysis was carried out between milk yield data from Holstein and DAK cows and expression levels of the lincRNAs and mRNAs. The findings showed that while lincRNAs and mRNAs associated with milk yield traits were upregulated …

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Bioaccumulation Monitoring Of Chemical Contaminants In Mussels Mytilus Galloprovincialis From The Southern Coast Of The Marmara Sea, Turkey, Serhat Çolakoğlu, İbrahi̇m Ender Küni̇li̇, Fatma Çolakoğlu Jan 2020

Bioaccumulation Monitoring Of Chemical Contaminants In Mussels Mytilus Galloprovincialis From The Southern Coast Of The Marmara Sea, Turkey, Serhat Çolakoğlu, İbrahi̇m Ender Küni̇li̇, Fatma Çolakoğlu

Turkish Journal of Veterinary & Animal Sciences

Mytilus galloprovincialis , the Mediterranean mussel, is an important shellfish species that constitutes the majority of production and consumption among bivalve mollusks in Turkey. Since shellfish can accumulate toxic chemicals from seawater, it is important to monitor bioaccumulation from their natural beds. For this reason, in this study, the aim was to monitor the levels of dioxins and dioxin-like polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and toxic metals (Pb, Cd, and Hg) in M. galloprovincialis collected from the southern coast of the Marmara Sea from 2014 to 2017. According to the results, the maximum levels of dioxins, PAHs, and …

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Effects Of Boron Compounds And Ozonated Olive Oil On Experimental Microsporum Canis Infection In Rats, Ayhan Hi̇lal Gezer, Abuzer Acar Jan 2020

Effects Of Boron Compounds And Ozonated Olive Oil On Experimental Microsporum Canis Infection In Rats, Ayhan Hi̇lal Gezer, Abuzer Acar

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the treatment outcomes of topical application of boric acid, boron-doped gel, and ozonated olive oil in cases of Microsporum canis -induced dermatophytosis. Furthermore, the outcomes were compared to those of terbinafine administration. We included 39 female Wistar albino rats weighing 200-250 g and created an M. canis-infected area on the skin of their backs. The rats were clinically scored on days 0, 7, 14, 21, and 28 and underwent histopathological evaluation. All the treated groups demonstrated significantly lower clinical scores than the control group (P < 0.05). Fewer inflammation cells were observed in the samples of groups treated with 3% boric acid and sodium pentaborate pentahydrate gel than in those of the control group. According to the histopathological evaluation, the groups treated with 3% boric acid and sodium pentaborate pentahydrate gel were statistically different from the control and other treatment groups (P < 0.05). Our results indicated that treatment with 3% boric acid and sodium pentaborate gel was adequate in resolving M. canis -induced infection in rats. Therefore, gels containing 3% boric acid and sodium pentaborate pentahydrate may be alternatives to antifungal agents such as terbinafine by ensuring easy, reliable, inexpensive, and effective treatment modalities.

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Polymorphism Of The Stat5a And Myf-5 Genes In Anatolian Water Buffalo, Fadi̇me Daldaban, Korhan Arslan, Esma Gamze Aksel, Bi̇lal Akyüz Jan 2020

Polymorphism Of The Stat5a And Myf-5 Genes In Anatolian Water Buffalo, Fadi̇me Daldaban, Korhan Arslan, Esma Gamze Aksel, Bi̇lal Akyüz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the genetic variation of MYF-5 and STAT5A genes in Anatolian water buffalo which was the only buffalo breed reared in Turkey by using the PCR-RFLP method. In this study, 120 Anatolian water buffalo were examined. After PCR amplification for MYF-5 gene, 512 bp PCR products were digested with TaqI enzyme. Although no AA genotype was found, the frequency of GG and AG genotypes were 0.77 and 0.33. PCR products of 215 bp for STAT5A gene were digested with AvaI enzyme and showed that all of the Anatolian water buffalo examined had monomorphic …

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The Potential Of Salvia Officinalis As A Suppressor Of Cell Proliferation In Animal Feed And Human Nutrition: An Experimental Stud, Muhammet Kuddusi̇ Erhan Jan 2020

The Potential Of Salvia Officinalis As A Suppressor Of Cell Proliferation In Animal Feed And Human Nutrition: An Experimental Stud, Muhammet Kuddusi̇ Erhan

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to examine the in vitro cytotoxic activities of Salvia officinalis (sage) oil on human immortalized keratinocyte (HaCaT) cell lines by using an [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] (MTT) cytotoxicity assay after the Salvia officinalis oil administration in different doses and time-points. In vitro cytotoxic activities of Salvia officinalis oil on HaCaT cell lines were assessed, and MTT assays were used to determine cell viability. The HaCaT cells (100 µL) were cultured in 96well plates at 2 × 104 cells per well and treated with different concentrations of Salvia officinalis oil (25 µM, 50 µM, 100 µM, 150 µM, …

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Effect Of Supplementing Additives In Leptin-Enriched Maturation Medium During In Vitro Maturation And Vitrification Of Goat Oocytes, Reema Talukdar, Ranjan Kumar Biswas, Govindasamy Kadirvel, Bharat Chandra Deka, Sudip Sinha, Lukumoni Buragohain, Birina Bora Jan 2020

Effect Of Supplementing Additives In Leptin-Enriched Maturation Medium During In Vitro Maturation And Vitrification Of Goat Oocytes, Reema Talukdar, Ranjan Kumar Biswas, Govindasamy Kadirvel, Bharat Chandra Deka, Sudip Sinha, Lukumoni Buragohain, Birina Bora

Turkish Journal of Veterinary & Animal Sciences

The present study aimed to evaluate the effect of supplementing epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), fetal bovine serum (FBS), and bovine serum albumin (BSA) in different combinations in a maturation medium (MM) comprising TCM-199 + 5 µg/mL 17-ß estradiol + 5 µg/mL ovine-luteinizing hormone (oLH) + 5 µg/mL pure follicle-stimulating hormone (pFSH) + 100 µM/mL cysteamine containing the best concentration of leptin among 10/20/25/30 ng/mL on in vitro maturation (IVM) and subsequent vitrification of matured goat oocytes. The IVM of oocytes was carried out in a CO2 incubator for 27 h at 38.5 °C. The percentage of …

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Growth Performance, Survival Rate, Flesh, And Proximate Composition Of Sex-Grouped Triploid And Diploid Nile Tilapia (Oreochromis Niloticus), Akhmad Taufiq Mukti, Odang Carman, Alimuddin Alimuddin, Muhammad Zairin Jr., Muhammad Agus Suprayudi Jan 2020

Growth Performance, Survival Rate, Flesh, And Proximate Composition Of Sex-Grouped Triploid And Diploid Nile Tilapia (Oreochromis Niloticus), Akhmad Taufiq Mukti, Odang Carman, Alimuddin Alimuddin, Muhammad Zairin Jr., Muhammad Agus Suprayudi

Turkish Journal of Veterinary & Animal Sciences

This study aimed to compare the growth performance, survival rate, flesh, and proximate composition of sex-grouped triploid and diploid Nile tilapia. The triploid population was obtained through heat shock at 41 ºC for 4 min, 4 min after fertilization. Before sexing, 50 fish were reared in aquaria at a density of 1 fish L$^{-1}$ for 2 months. After sexing, both triploid and diploid fish were grouped into all-male, all-female, and mixed-sex groups and reared in hapas at a density of 10 fish m$^{-2}$ for 4 months. Each group was replicated three times. The highest body weight, body length, and growth …

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