Digital Commons Network^™

Alternatives To Antibiotics In Veterinary Medicine: Considerations For The Management Of Johne's Disease, Laura M. O'Connell, Aidan Coffey, Jim O'Mahony

Dept. of Biological Sciences Preprints

Antibiotic resistance has become a major health concern globally, with current predictions expecting deaths related to resistant infections to surpass those of cancer by 2050. Major efforts are being undertaken to develop derivative and novel alternatives to current antibiotic therapies in human medicine. What appears to be lacking however, are similar efforts into researching the application of those alternatives, such as (bacterio)phage therapy, in veterinary contexts. Agriculture is still undoubtedly the most prominent consumer of antibiotics, with up to 70 % of annual antibiotic usage attributed to this sector, despite policies to reduce their use in food animals. This not …

Microbiological Safety And Quality Of Ceviche, Poke, And Sushi Dishes Sold At Retail Outlets In Orange County, Ca, Grace E. Marquis, Samantha M. Covaia, Amanda M. Tabb, Courtney J. Kitch, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Raw, ready-to-eat (RTE) seafood products, such as ceviche, poke, and sushi, have experienced growing demand globally; however, these products have the potential to be contaminated with foodborne pathogens. The objective of this study was to determine the prevalence of Escherichiacoli/coliforms, Salmonella, and Listeria in ceviche, poke, and sushi dishes sold at the retail level in Orange County, CA, USA. Additional organisms detected during testing were also considered in the results. A total of 105 raw, RTE samples of ceviche, poke, and sushi were collected from restaurants and grocery stores in Orange County, CA. Samples were tested for Salmonella …

Reproduction, Early Development, And Larval Rearing Of The Climbing Perch Anabas Testudineus (Teleostei: Anabantidae) In Captivity, Frolan A. Aya, Reylan C. Gutierrez, Luis Maria B. Garcia

The Philippine Agricultural Scientist

Induced spawning, early development, and larval rearing of the climbing perch Anabas testudineus were examined under captive conditions. Five female climbing perch [125.33 ± 3.27 mm SL; 70.67 ± 5.59 g BW] were paired with apparently mature males (108.50 ± 3.97 mm SL; 39.27 ± 4.70 g BW) and induced to spawn with 5,000 IU human chorionic gonadotropin (hCG) + 0.5 mL Ovaprim kg BW^-1. All hCG + Ovaprim-injected fish spawned 9 – 11 h after hormone administration but saline-injected fish (control group) failed to spawn. Mean egg production per female, fertilization and hatching rates, and larval production …

Effect Of Vitamin C And Probiotics On Broilers Performance And Claudin-2 (Cldn-2) Expression Under Heat Stress [Research Note], Mohammad Chamani, Mohammad Chamani, Farhad Foroudi, Ali Asghar Sadeghi, Mehdi Aminafshar

The Philippine Agricultural Scientist

This study was conducted to investigate the effect of vitamin C and probiotic which consists of five species of beneficial bacteria and two species of fungi on performance, blood parameters, and intestinal CLDN-2 gene expression of broilers during heat stress (HS). A total of 400 broilers were randomly divided into five groups, each of which received a different treatment. The first group was considered a negative control and did not experience any HS (C^-); the second group was considered a positive control (C⁺) and was exposed to HS; the third group received only vitamin C (250 …

Effect Of Use Of Tsetse Repellant Collar Technology On The Farm Performance And Household Welfare Of Small-Scale Livestock Farmers In Kenya, Beatrice W. Muriithi, Kassie Menale, Gracious M. Diiro, Michael N. Okal, Daniel K. Masiga

All Peer-Reviewed Publications

Tsetse-transmitted Animal African Trypanosomosis (AAT) is a disease of economic importance to livestock development in Sub-Saharan Africa. The disease is widely managed using trypanocides that are expensive for smallholders and associated with health risks due to drug residues in animal products and drug resistance. Developed recently by the International Centre of Insect Physiology and Ecology (icipe) and partners, the novel Tsetse repellent collar technology (TRCT) provides an effective alternative control measure that targets the tsetse fly vector of AAT. This study assessed the impact of TRCT on household welfare and food security by using ex-post non-experimental data obtained from 632 …

Peripheral And Central Impact Of Methionine Source And Level On Growth Performance, Circulating Methionine Levels And Metabolism In Broiler Chickens, Craig W. Maynard, Elizabeth Gilbert, Frances Yan, Mark A. Cline, Sami Dridi

Poultry Science Faculty Publications and Presentations

The present study was designed to evaluate the effects of DL-methionine (DL-Met) 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), or S-(5′-Adenosyl)-L-methionine chloride (SAM), using feeding trial and central administration, on live performance, plasma metabolites, and the expression of feeding-related hypothalamic neuropeptides in broilers raised to a market age (35 d). Final average body weight (BW) and feed conversion ratio (FCR) from the feeding trial exceeded the performance measurements published by the primary breeder. At d35, the MTBHa group had better BW and lower feed intake, which resulted in a better FCR than the DL-Met group at 87 TSAA to lysine. At the molecular …

Impact Of Phytase Supplementation On Meat Quality Of Heat-Stressed Broilers, C. J. Maynard, C. W. Maynard, Garrett J. Mullenix, Alison Ramser, Elizabeth S. Greene, Mike R. Bedford, Sami Dridi

Poultry Science Faculty Publications and Presentations

Heat stress (HS) is one of the most challenging stressors to poultry production sustainability. The adverse effects of HS range from feed intake and growth depression to alteration of meat quality and safety. As phytase supplementation is known to improve nutrient utilization and consequently growth, we undertook the present study to evaluate the effects of dietary phytase on growth and meat quality in heat-stressed broilers. A total of 720 day-old hatch Cobb 500 chicks were assigned to 24 pens within controlled environmental chambers and fed three diets: Negative Control (NC), Positive Control (PC), and NC diet supplemented with 2000 phytase …

Integrative Analyses Of Genomic And Metabolomic Data Reveal Molecular Mechanisms Associated With Uterine Disease Traits In Transitioning Dairy Cattle, Leanna C. Sanchez, Mohammed Abo-Ismail, Daniel Peterson, Fernando Campos-Chillon

Master's Theses

The metritis complex (MC), characterizing post-partum uterine diseases in dairy cattle has negative implications on animal welfare, production efficiency, and the economic stability of the dairy industry. The studies in this thesis aimed to investigate the genetic architecture of the metritis complex and identify genomic regions and metabolites associated with the development of MC. Thereby enhancing our understanding of the biological pathways and molecular mechanisms involved in the pathophysiology of MC during the transition period in Jersey and Holstein dairy cattle.

Chapter 2 sheds light on the previous work done on MC. The goals of this review were to (1) …

Exploring The Use Of Innovative Sustainable Proteins In Marine Finfish Diets, Luke Pilmer, Lindsey Woolley, Md Reaz Chaklader

Conference papers and presentations

No abstract provided.

Rapid Assessment Of Fish Freshness For Multiple Supply-Chain Nodes Using Multi-Mode Spectroscopy And Fusion-Based Artificial Intelligence, Hossein Kashani Zadeh, Mike Hardy, Mitchell Sueker, Yicong Li, Angela Tzouchas, Nicholas Mackinnon, Gregory Bearman, Simon A. Haughey, Alireza Akhbardeh, Insuck Baek, Chansong Hwang, Jianwei Qin, Amanda M. Tabb, Rosalee S. Hellberg, Shereen Ismail, Hassan Reza, Fartash Vasefi, Moon Kim, Kouhyar Tavakolian, Christopher T. Elliott

Food Science Faculty Articles and Research

This study is directed towards developing a fast, non-destructive, and easy-to-use handheld multimode spectroscopic system for fish quality assessment. We apply data fusion of visible near infra-red (VIS-NIR) and short wave infra-red (SWIR) reflectance and fluorescence (FL) spectroscopy data features to classify fish from fresh to spoiled condition. Farmed Atlantic and wild coho and chinook salmon and sablefish fillets were measured. Three hundred measurement points on each of four fillets were taken every two days over 14 days for a total of 8400 measurements for each spectral mode. Multiple machine learning techniques including principal component analysis, self-organized maps, linear and …

Impact Of Cattle Grazing Corn Residue In Spring On Soil And Subsequent Soybean Yield, Morgan T. Grabau, J. C. Macdonald, Zach E. Carlson, M. E. Drewnoski

Department of Animal Science: Faculty Publications

The effects of spring corn (Zea mays) residue grazing and stocking density on soil physical properties and subsequent soybean (Glycine max) yield were evaluated in an experiment with three treatments: no grazing (NG), normal stocking density (NSD), and high stocking density (HSD). The study was conducted over 2 years with four replicates per treatment each year. Steers (277 ± 4.4 kg) were stocked at 7.5 steers/ha for NSD with a target grazing of 45 days starting in mid- February, and 22.5 steers/ha for HSD with a target grazing of 15 days in mid-March, such that the …

Comparing True Maternal And Paternal Care In Pair-Living, Ex-Situ Varecia Rubra (Red Variegated Lemurs) Contextualized Among Other Primate Cooperative Breeders, Payton Elizabeth Zuver

Dissertations and Theses

Observations of variegated lemurs in the wild have revealed a cooperative breeding strategy including extensive male care to young, primarily in the form of infant guarding. This thesis presents an analysis of the first quantitative assessment of true paternal and true maternal care from pair-housed, ex-situ red variegated lemurs (V. rubra) housed at the Lemur Conservation Foundation's Myakka City Lemur Reserve in western Florida. This study aims to answer specific research questions: How do paternal and maternal care compare when resources are abundant, and paternity is certain? Is male care instinctual? Is it driven by energetic necessity? If …

Raising A Bottle-Fed Calf On A Cow/Calf Operation, Reganne K. Briggs, Katelyn Barsnick, Cheyenne Reid, Ashley Longmore

All Current Publications

Having bottle-fed calves on the ranch is common for beef producers. The most likely scenarios are a cow has died, a cow isn’t giving enough milk, or a twin has been rejected. All these scenarios are likely to happen, which results in an orphan calf. Bottle-feeding a calf is straightforward; however, there are limited resources, specifically for beef producers, looking for bottle-fed calf management strategies. When bottle-feeding a beef calf, there are some things to consider, such as feeding colostrum, selecting and using milk replacers, and weaning the calf. Many of the resources on bottle-calf management come from the dairy …

Evaluation Of The Relationship Between Hair Coat Shedding Ability, Apparent Forage Digestibility, And Mineral Status In Angus Cattle, Margaret P. Loftin

Theses and Dissertations

The objective of this study was to evaluate the relationship between hair coat shedding ability, apparent forage digestibility, and mineral status in Angus cattle. Fecal samples were collected on fall calving Angus cows for one week in March, May, and July from 2019-2022. Liver and plasma samples were collected in March and July each year. Fecal samples were analyzed for DM, ash, NDF, and ADF while liver and plasma were analyzed for mineral concentration. Data were analyzed using MIXED procedure of SAS with significance declared at P0.05. Interactions were detected for DM, NDF, ADF, and liver Fe concentration. The model …

Molecular Characterization Of Seminal Plasma From Boars With Divergent Sperm Quality, Notsile Hleliwe Dlamini

Theses and Dissertations

Seminal plasma (SP) is the microenvironment of spermatozoa whose composition reflects semen quality and constitutes an excellent source for detecting non-invasive biomarkers. This study characterizes good vs. poor quality semen using seminal extracellular vesicles coupled proteins and miRNA. Fresh boar semen samples were screened, centrifuged, and SP supernatants were collected for near-infrared spectroscopy (NIRS) analysis. EVs were extracted from SP (SP-EVs), characterized, and those of extremely poor or good sperm quality groups were subjected to proteomics and small RNA sequencing. Significant differences were set for P

Evaluation Of Nutrient Digestibility Of Weaned Calves From Early And Late Shedding Dams, Jennifer Keele

Theses and Dissertations

Researchers have investigated several factors that could alter fetal growth, including nutrient restriction (Valiente et al., 2021), hair shedding (Gray et al., 2011), and extreme hot and cold temperatures (Davidson et al., 2022). Hot temperatures and increased humidity percentages in the southeast United States caused researchers to investigate the hair coats of Angus cattle in the commercial production setting. An improvement in fiber digestibility and calf birth and weaning weights has been observed in Angus dams that shed 50% of the winter hair coat by May (Gray et al., 2011; Burnett et al., 2021). Our objective of this experiment was …

Roles Of Limestone Particle Size Ratios And Phytase Levels On Performance, Eggshell Quality, And Bone Health In Post-Peak And Late-Lay Single-Cycled W-36 Laying Hens, Charis Waters

Theses and Dissertations

The objective of this study was to investigate the role of fine (F) and coarse (C) limestone ratios and exogenous phytase at standard and superdosing activity on performance, egg quality, and bone quality of post-peak and late-lay Hy-Line W-36 hens. For post-peak, treatments with 40F:60C at 0 and 400 FTU/kg (standard phytase activity) increased hen-day egg production (HDEP). Treatments with 15F:85C decreased feed intake (FI) but also increased unsaleable eggs (UE) and eggshell-breaking strength (EBS). Treatments with 0 and 400 FTU/kg increased albumen quality and treatments with 1500 FTU/kg increased bone quality and phytate breakdown. For late-lay, interaction effects of …

Remote Physiological Monitoring Of The Giant Panda And Red Panda Using Near Infrared Spectroscopy And Machine Learning, Qingyu Sheng

Theses and Dissertations

Appropriate conservation decisions and efforts must be based on real−time and accurate information about wildlife populations. However, it is extremely challenging to monitor the population demography and physiological traits of many threatened and secretive animal species through direct observation and capture. Near infrared spectroscopy (NIRS) has the potential to be a remote tool to address questions concerning wildlife physiology and demography by analyzing “signs” of animals without seeing or capturing them. In this dissertation, two species, the giant panda (Ailuropoda melanoleuca) and red panda (Ailurus fulgens) are used as a case study, to demonstrate NIRS’ feasibility …

Identifying Barriers To Data Use On U.S. Beef Cow-Calf Operations And Developing Solutions To Improve Cow-Calf Record-Keeping, William Isaac Jumper

Theses and Dissertations

Cattle health and production records (CHPR) are data collected by cattle producers and veterinarians in the form of measurements, observations, counts of events over time, and physiologic attributes that describe individual and group-level health and production. These data are useful to both veterinarians and cattle producers for making evidence-based decisions on cow-calf operations. Currently, there are no uniform, industry-wide methods of capturing and recording CHPR in the U.S. cow-calf industry. Although many cow-calf producers in the U.S. are thought to collect some form of CHPR, it is believed that relatively few are doing so in an electronic manner that facilitates …

Evolution And Epidemiology Of Channel Catfish Virus (Ccv), Arun Venugopalan

Theses and Dissertations

Channel catfish virus disease (CCVD) is the principal viral disease in the United States catfish industry. The CCVD is caused by channel catfish virus (CCV), with mortality reaching up to 100% in fingerlings. CCV is assigned taxonomically to the family Alloherpesviridae, genus Ictalurivirus, species Ictalurid herpesvirus 1 (IcHV-1). To date, virulence, immunogenicity, and genome plasticity of the CCV field isolates have not been investigated. Three genotypes of CCV (IcHV-1A, IcHV-1B, and BCAHV) were identified using restriction fragment length polymorphism (RFLP) analysis. Virulence assessment of three representative isolates of RFLP groups suggests that IcHV-1B (pooled survival [mean ± SE]: 58.3% ± …

Utilizing NuTrack To Access The Activity Levels In Pigs With Varying Degrees Of Genetic Potential For Growth And Feed Intake, Dalton Obermier, Melanie Trenahile-Grannemann, Ty B. Schmidt, Tom Rathje, Benny Mote

Department of Animal Science: Faculty Publications

Feed cost accounts for over two-thirds of the variable cost of production. In order to reduce feed costs without sacrificing production numbers, feed efficiency must be improved. Calorie expenditure has been difficult to quantify in the past but is understood to impact residual feed intake (RFI) greatly. The objective of this work was to utilize an advanced computer vision system to evaluate activity levels across sex and sire groups with different expected breeding value combinations for growth and feed intake. A total of 199 pigs from four different sire groups (DNA Genetics Line 600) High Feed Intake/High Growth (HIHG), Low …

Channel Island Foxes (Urocyon Littoralis) Are Viable Seed Dispersal Agents Of Toyon, Savannah L. Bartel, Juliann T. Schamel, Bridget A. Parrino, Melissa K. Marshall, John L. Orrock

Western North American Naturalist

Mammals that consume fleshy fruit and deposit viable seeds in their scat can be important agents of seed dispersal for plants. Despite the knowledge that the island fox (Urocyon littoralis) consumes large quantities of fleshy fruits, it is unknown whether seeds survive digestion by island foxes and are able to germinate. In order to evaluate whether island foxes are agents of viable seed dispersal on the Channel Islands, we collected island fox scat on Santa Cruz Island in February 2021 and conducted germination assays on digested seeds found in scat. We compared the germinability and germination rates of …

Appearance Of Choline Metabolites In Plasma And Milk When Choline Is Infused Into The Abomasum With Or Without Methionine, C. J.R. Jenkins, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Four lactating, ruminally cannulated Jersey cows, (mean ± standard deviation) 264 ± 54.2 d in milk and 484 ± 24.1 kg of body weight, were arranged in a 4 × 4 Latin square design to measure the effects of abomasal infusion of choline chloride with or without dl-Met on milk and plasma choline metabolites and plasma AA in cows fed a Met-deficient diet. Cows were randomly assigned to 1 of 4 experimental treatments: (1) control; no supplemental Met or choline (CON), (2) 13 g/d of choline ion delivered via abomasal infusion (CHO), (3) 13 g/d of Met delivered via abomasal …

Effects Of Exercise When Transitioning From Free Choice Tall Fescue (Lolium Arundinaceum) Grass Diet In Late Summer To Free Choice Tall Fescue Hay Diet In Late Fall On Plasma Fructosamine Concentrations, Insulin Levels, Body Weight, And Body Condition Scores Of Stock Horse Mares And Gelding, Riley Brown

Masters Theses & Specialist Projects

Equine exercise may influence a horse’s metabolic and physiological functions. Studies can help to prove this linkage, and to better understand the nutritional requirements of the horse. The parameters that are being evaluated are plasma-fructosamine concentrations, insulin levels, body weight (lbs.), and body condition score (BCS) as a measurement of obesity. Eight horses used in this study were split into two groups; Worked horses were exercised three times a week while the unworked horses were not exercised. All eight horses were fed an ad libitum diet of tall fescue grass and hay for 127 days.

Forage samples were collected to …

Perspectives Of Dairy Employees At The Beginning Of The Covid-19 Pandemic: A Survey Of Health Risks And Educational Needs, Fernanda Ferreira, Maristela Rovai, Mireille Chahine, Mario E. De Haro-Martí, John Wenz, Joseph Dalton, Noelia Silva-Del-Rio

Dairy Science and Food Science Faculty Publications: 2022 to Present

Our objective was to understand dairy employees' perceptions and educational needs at the onset of the COVID-19 pandemic. A bilingual (English and Spanish), anonymous survey targeted at dairy employees was circulated nationwide via university and allied industry media outlets. Responses (n = 63) from 11 states were received (May–Sep. 2020). Respondents worked in herds ranging from 50 to 40,000 animals in size. Dairy managers (33%) responded mostly to the English survey (52%), whereas entry-level workers (67%) chose the Spanish format (76%). Survey results highlighted different perspectives, educational needs, and preferred sources of information between English- and Spanish-speaking dairy workers. Overall, …

Determining The Effectiveness Of Rosemary Essential Oil On The Shelf Life Of Ground Beef Under Different Lighting Conditions, Jordan Looper

Animal Science Undergraduate Honors Theses

The objective of this study was to determine the effectiveness of rosemary extract on the shelf life of ground beef patties under different retail display conditions. Ground beef patties were produced from an 85% lean and 15% fat blend purchased from a local grocery retailer, finely ground through a 0.953 cm plate and separated into 151.2 g patties (n=64). Patties were randomly assigned into a control group (no antioxidants added) or a 0.20% concentration rosemary extract group. Patties were individually packaged using polyvinyl chloride overwrap. Antioxidant and control patties were randomly assigned into one of two lighting intensity …

Pharmacokinetics Of Cbd Supplementation In Horses: Single Dose Vs. Long-Term Feeding, Madilyn Adamchik

Honors College Theses

Research about supplemental feeding of cannabidiol (CBD) to horses is still in its early stages, but many horse owners are feeding CBD supplements for supposed benefits, including behavior and pain management. Cannabinoids work through the endocannabinoid system to modulate nervous and hormonal actions in the body, working to maintain a healthy homeostatic balance. Safety and efficacy have been tested for dosages of up to 500 mg of CBD per day for horses, but further research about the pharmacokinetics based on dosage amount and form of CBD are still needed. The objectives of this study were to evaluate the pharmacokinetics of …

Effect Of Soy Co-Products In Supplements For Grazing Cattle On Growth, Complete Blood Cell Counts, And Physiological Response Following A Lipopolysaccharide Challenge, Bralee Lansdell

Biological Sciences Undergraduate Honors Theses

The objective of this study was to determine the effect of soy co-products (soybean meal and soy oil) in the diet on the growth and hemocytology of cattle during a 56-day growing phase and the physiological/behavioral response to an endotoxin challenge. Angus crossbred steers (n = 36; 289 ± 31 kg, initial body weight ± SD) were stratified by body weight and sire; and assigned randomly to pastures (n = 9; 0.45 ha/mixed-grass pasture). Pastures were assigned randomly to 1 of 3 dietary treatments: 1) a control supplement containing no soy co-products, 2) a supplement containing soybean meal, or 3) …

Environmental Drivers Of Stress In Red Pandas (Ailurus Fulgens) : Behavior And Space Use Changes In Response To Redesigned Naturalistic Enclosures, Alexis Michelle Lawson

Theses, Dissertations and Culminating Projects

Animal welfare encompasses the physical and mental state of an animal, as well as how that animal is cared for. Recently, zoological settings have shifted from strictly housing animals to improving the welfare of the animal such that the goal of captivity is for residents to thrive. This is especially crucial as global wildlife numbers and biodiversity continues to decrease due to anthropogenic impacts such as poaching, mining, habitat destruction, fragmentation. Further confounding this issue is climate change, air pollution, invasive species, ocean acidification, and more. In particular, the red panda (Ailurus fulgens) is an endangered species whose declined 50% …

Early Solid Diet Supplementation Influences The Proteomics Of Rumen Epithelium In Goat Kids, Yimin Zhuang, Xiaokang Lv, Kai Cui, Jianmin Chai, Naifeng Zhang

Animal Science Faculty Publications and Presentations

It is well known that solid diet supplementation in early life can significantly promote rumen development and metabolic function in young ruminants. However, the changes in the expressed proteome and related metabolism in rumen epithelium in response to a supplemented solid diet remain unclear. In this study, rumen epithelial tissue from goats in three diet regimes including milk replacer only (MRO), milk replacer supplemented concentrate (MRC), and milk replacer supplemented concentrate plus alfalfa pellets (MCA) were collected for measurement of the expression of epithelial proteins using proteomic technology (six per group). The results showed that solid diet significantly improved the …