Digital Commons Network^™

Host-Parasite Associations Of Small Mammal Communities And Implications For The Spread Of Lyme Disease, Matthew J. Buchholz

Masters Theses & Specialist Projects

Many zoonotic pathogens of concern to human and veterinary health are maintained in the environment within small mammal reservoirs and vectored to new hosts by ectoparasitic arthropods. While the ecological relationships among small mammals, ectoparasites, and disease-causing symbiotic microorganisms are important to these dynamics, little is known about them across much of North America. The sylvatic cycle of Borrelia burgdorferi, the etiologic agent of Lyme disease, is of particular interest because Lyme disease is the most common vector-borne disease of humans in the United States. However, cases of Lyme disease are primarily confined to the northeastern and Midwestern United States, …

How Microbial Communities Differ In Ruminants On A Glycerin Vs Common Diet, Makala Muller, Nirosh D. Aluthge, Allison L. Knoell, Morgan Kaiser, Samodha C. Fernando

UCARE: Research Products

Can we reduce methane by using dietary interventions to reduce carbon footprint and increase feed efficiency? Ruminant livestock account for a significant amount of the anthropogenic methane produced in the U.S. All ruminant livestock produce 28% of the earth’s methane emissions. The methane within ruminants is produced by a microbial fermentation, especially by a group of bacteria known as the methanogens. However, the different species of methanogens and their pathways of methane production within ruminants are not fully understood. Cattle are considered one of the main producers of methane. They produce 20% of the U.S. methane emissions. Methane absorbs more …

Examining Maternal Obesity Effects On Oogenesis, Follicular Development, And Embryonic Myogenesis, Chris Lindeman, Jennifer Wood

UCARE: Research Products

The CDC estimates that more than one-third of U.S. adults (approximately 78.6 million) are obese. Despite national efforts to combat this with diet and exercise, the number of obese adults and children continues to climb. It is critical to emphasize that obesity can lead to lifelong, chronic complications and health risks including hypertension, heart disease, and diabetes.

Diet and obesity also have important impacts on the reproductive health of female including the ability to become pregnant (fertility) and risk for complications during pregnancy. Furthermore, for women who do become pregnant, obesity can affect the development of the embryo and fetus, …

Beef Systems Management Strategies: Calving Date Selection, Estrus Synchronization, And Post-Weaning Management, Benjamin T. Tibbitts

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of an invited review and four experiments was to evaluate production implications and economic efficiencies of beef systems strategies involving calving date selection, utilization of reproductive technology, and post-weaning management of heifers and steers. The invited review in chapter II considers factors that influence a producer’s decision on when to calve beef females. The calving date decision impacts the entire beef production cycle and must take into account any environmental conditions, available resources, and cite specific advantages and/or disadvantages. Understanding the importance of varying physiological state and nutrient demands associated with lactation and gestation is critical to optimizing …

The Effect Of Diet On The Bovine Rumen Microbial Community Structure And Composition And Its Effects On Methane Production In Growing And Finishing Cattle, Allison L. Knoell

Department of Animal Science: Dissertations, Theses, and Student Research

Methane is a potent greenhouse gas (GHG) that contributes to global warming. A natural by-product of ruminant fermentation is the production and eructation of methane. Methane is produced by a small unique group of microorganism’s called methanogens that belong to the domain Archaea. Enteric methane represents 2-12% energy loss in ruminants. It is well established that diet affects the microbial community structure and composition. Fermentative products of the mixed microbial population (bacteria, fungi, and protozoa) become the substrates for methanogens. These substrates influence which microorganisms will thrive. However, the effect of diet on the microbial community while simultaneously calculating …

Section Abstracts: Agriculture, Forestry And Aquaculture

Virginia Journal of Science

Abstracts of the Agriculture, Forestry and Aquaculture Section for the 94th Annual Virginia Academy of Science Meeting, May 18-20, 2016, at University of Mary Washington, Fredericksburg, VA.

Is Diet Selection By Greater Sage-Grouse Influenced By Biomass Availability Or Toxins?, Jacqueline Peña, Marcella Fremgen

McNair Scholars Research Journal

Foraging herbivores must meet nutritional requirements by not only finding enough plant biomass to consume, but also finding plants with high protein content and low concentrations of potentially toxic plant secondary metabolites (PSMs). Greater sage-grouse (Centrocercus urophasianus; hereafter, sage-grouse) are sagebrush obligate herbivores that consume relatively high concentrations of PSMs. To meet their nutritional needs and avoid ingesting high amounts of PSMs, sage-grouse may select species of sagebrush for food that have lower concentrations of PSMs than a more abundant species with higher concentration of PSMs. Diet selection by sage-grouse may also be driven by chemical factors at finer scales …

Growing South Dakota (Winter 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2015 SDSU Extension Annual Report.

[Page] 2 Contours Program Launched: Aims to Provide Insights & Prompt Discussions
[Page] 4 Local Momentum: Formation of State's First “Food Hub” Comes To Fruition [Page] 6 Spotlight On Soil Health: New Coalition Formed
[Page] 8 Exploring A Range Of Options: Youth Programs Cultivate Interest In Natural Resource Careers
[Page] 9 Profiles In Leadership: Our Mission Continues… Ensuring All Citizens Have Access To Education & Emerging Innovation
[Page] 10 Encouraging Animal Well-Being Workshops & Trainings Help Livestock Producers Become Better Stewards
[Page] 12 Wellness Wisdom: Variety Of Efforts Available To Encourage …

Effects Of Nutrition On Female Leopard Geckos, Lisa Keys, Dennis M. Ferraro

UCARE: Research Products

Nutrition has a dramatic effect on animal’s bodies and behaviors as they go through their lives. Looking at the effects of nutrition on captive animals is crucial to providing future animals with better lives. Since captive animals don’t get the variety of food items that they would get in the wild, supplementation is often used to meet the minimum dietary requirements. Looking at how supplementation affects female leopard geckos at breeding size, there were both positive and negative correlations with different supplementations used. After taking body measurements on females over several months on one kind of additional supplement, the control …

Intrauterine Growth-Restricted Sheep Fetuses Exhibit Smaller Hindlimb Muscle Fibers And Lower Proportions Of Insulin-Sensitive Type I Fibers Near Term, Dustin T. Yates, Caitlin N. Cadaret, Kristin A. Beede, Hannah E. Riley, Antoni R. Macko, Miranda J. Anderson, Leticia E. Camacho, Sean W. Limesand

Department of Animal Science: Faculty Publications

Intrauterine growthrestricted sheep fetuses exhibit smaller hindlimb muscle fibers and lower proportions of insulin-sensitive Type I fibers near term. Am J Physiol Regul Integr Comp Physiol 310: R1020–R1029, 2016. First published April 6, 2016; doi:10.1152/ajpregu.00528.2015.—Intrauterine growth restriction (IUGR) reduces muscle mass and insulin sensitivity in offspring. Insulin sensitivity varies among muscle fiber types, with Type I fibers being most sensitive. Differences in fibertype ratios are associated with insulin resistance in adults, and thus we hypothesized that near-term IUGR sheep fetuses exhibit reduced size and proportions of Type I fibers. Placental insufficiency-induced IUGR fetuses were 54% smaller (P < 0.05) than controls and exhibited hypoxemia and hypoglycemia, which contributed to 6.9- fold greater (P < 0.05) plasma norepinephrine and 53% lower (P < 0.05) plasma insulin concentrations. IUGR semitendinosus muscles contained less (P < 0.05) myosin heavy chain-I protein (MyHC-I) and proportionally fewer (P < 0.05) Type I and Type I/IIa fibers than controls, but MyHC-II protein concentrations, Type II fibers, and Type IIx fibers were not different. IUGR biceps femoris muscles exhibited similar albeit less dramatic differences in fiber type proportions. Type I and IIa fibers are more responsive to adrenergic and insulin regulation than Type IIx and may be more profoundly impaired by the high catecholamines and low insulin in our IUGR fetuses, leading to their proportional reduction. In both muscles, fibers of each type were uniformly smaller (P < 0.05) in IUGR fetuses than controls, which indicates that fiber hypertrophy is not dependent on type but rather on other factors such as myoblast differentiation or protein synthesis. Together, our findings show that IUGR fetal muscles develop smaller fibers and have proportionally fewer Type I fibers, which is indicative of developmental adaptations that may help explain the link between IUGR and adulthood insulin resistance

Low-Intensity, Stocker-Based Channel Catfish Culture, Bartholomew W. Green, Carole R. Engle, Rebecca Lochmann, Harold Phillips

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Low-intensity production of Channel Catfish Ictalurus punctatus is characterized by low stocking rates, low installed aeration capacity, and no automated dissolved oxygen monitoring. Two studies conducted in nine 0.25-acre ponds quantified production characteristics of stocker Channel Catfish stocked for low-intensity food-fish production in single- or multiple-batch culture. In the multiple-batch study, 0.5-lb stockers (carryover fish) were stocked at 4,500 fish/acre and 0.044-lb fingerlings at 6,000 fish/acre. Stockers were grown to mean individual weights of 1.25, 1.75, or 2.25 lb. In the single-batch study, 0.5-lb stockers were stocked at 3,500, 4,500, and 5,500 fish/acre. All fish were fed a feed containing …

Mitigation Of Ergot Vasoconstriction By Clover Isoflavones In Goats (Capra Hircus), Glen E. Aiken, Michael D. Flythe, Isabelle A. Kagan, Huihua Ji, Lowell P. Bush

Kentucky Tobacco Research and Development Center Faculty Publications

Ergot alkaloids produced by a fungal endophyte (Epichloë coenophiala; formerly Neotyphodium coenophialum) that infects tall fescue (Lolium arundinaceum) can induce persistent constriction of the vasculature in ruminants, hindering their capability to thermo-regulate core body temperature. There is evidence that isoflavones produced by legumes can relax the vasculature, which suggests that they could relieve ergot alkaloid-induced vasoconstriction and mitigate the vulnerability to severe heat stress in ruminants that graze tall fescue. To test if isoflavones can relieve alkaloid-induced vasoconstriction, two pen experiments were conducted with rumen-fistulated goats (Capra hircus) to determine with ultrasonograpy if …

Use Of The Mitochondrial Control Region As A Potential Dna Mini-Barcoding Target For The Identification Of Canned Tuna Species, Jacquelyn K. Mitchell, Rosalee S. Hellberg

Food Science Faculty Articles and Research

In this study, a DNA mini-barcoding methodology was developed for the differentiation of species commonly found in canned tuna. Primers were designed to target a 236-base pair (bp) fragment of the mitochondrial control region (CR) and a 179-bp fragment of the first internal transcribed spacer region (ITS1). Phylogenetic analysis revealed the ability to differentiate 13 tuna species on the basis of the CR mini-barcode, except in a few cases of species introgression. Supplementary use of ITS1 allowed for differentiation of introgressed Atlantic bluefin tuna (Thunnus thynnus) and albacore tuna (Thunnus alalunga), while differentiation of introgressed Atlantic …

Animal Suffering And Human Bias, Stijn Bruers

Animal Sentience

Ng proposes concrete ways to decrease animal suffering on the basis of commonsense economic logic and research in welfare biology. But to reduce animal suffering effectively in livestock farming, animal experimentation or the natural environment we have to become more aware of our pervasive and spontaneous but unreliable intuitive moral judgments. These can generate biases that prevent us from decreasing animal suffering effectively.

Circulating Amino Acids In Blood Plasma During The Peripartal Period In Dairy Cows With Different Liver Functionality Index, Z. Zhou, J. J. Loor, F. Piccioli-Cappelli, F. Librandi, G. E. Lobley, E. Trevisi

Physiology Collection

The liver functionality index (LFI) measures the changes of albumin, cholesterol, and bilirubin concentrations between 3 and 28 d postpartum. This composite index, based on variables with direct relevance to liver-specific plasma protein synthesis (albumin), hepatic/intestinal lipoprotein synthesis (cholesterol), and clearance of breakdown products of heme catabolism (bilirubin), provides a tool for evaluating manifestations of hepatic disease. Both energy and protein metabolism are likely to be affected by various physiological challenges in this period but have not been tested systematically. The present study was conducted to profile AA in cows with high or low LFI during the peripartal period …

Challenges And Opportunities In The Culture Of Giant Freshwater Prawn In The Philippines, Janice A. Ragaza

Biology Faculty Publications

Amid the irremediable decline of capture fisheries, the Philippine government has identified aquaculture as a sector that will ensure food security and generate new job opportunities geared toward the country’s goal of economic development (Nagothu and Ortiz 2007). Aquaculture of giant freshwater prawn Macrobrachium rosenbergii (Fig. 1) is an emerging program that is intended to expand and vary the aquatic species used in freshwater aquaculture, which is still predominantly based on tilapia (Rosario and Tayamen 2004). Attractive characteristics of freshwater prawn culture include ease of breeding, high hatching rate, superior quality of meat and high market value (Keysami and Mohammadpour …

Life Line March 2016, Otterbein Biology And Earth Science Department

Life Line - The Biology Department Newsletter

Department welcomes Dr. Elizabeth Berkeley - Ben Titus '08 - Panama Research - Fracing in Toga County, PA. - Students Intern in China - Faculty Partnerships in Africa

Gascoyne Demersal Scalefish Managed Fishery, Department Of Fisheries

Fisheries Occasional Publications

An operators’ guide to the management arrangements 1 September 2015 – 31 August 2016.

Effect Of Grazing Prairie Dog—Colonized Rangeland On Cattle Nutrition And Performance: A Progress Report, Kenneth C. Olson, Christopher Schauer, Chanda Engel, Janna J. Kincheloe, Jameson R. Brennan, Ben L. Hauptman

Natural Resource Management Faculty Publications

On the Ground

• One objective of the ongoing Renewal on Standing Rock Reservation project is to evaluate the response of grazing steers to the level of prairie dog colonization on Northern Mixed Grass Prairie.
• We fenced four pastures to create an increasing gradient of a proportion of the pasture area colonized by prairie dogs. Pastures are stocked with yearling steers during each growing season.
• Comparing steer performance, Global Positioning System (GPS) locations of grazing, diet samples, and ingestive behavior at each proportion of the prairie dog colony per pasture allows prediction of the optimal proportion of colonization, which enables selection …

Hepatic Transcriptomic And Metabolic Responses Of Hybrid Striped Bass (Morone Saxatilis × Morone Chrysops) To Acute And Chronic Hypoxic Insult, Benjamin H. Beck, S. Adam Fuller, Bartholomew W. Green, Honggang Zhao, Steven D. Rawles, Carl D. Webster, Eric Peatman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of fish prized for recreational angling in the United States, and there and abroad as a high-value farmed fish. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we examined the …

Hepatic Transcriptomic And Metabolic Responses Of Hybrid Striped Bass (Morone Saxatilis × Morone Chrysops) To Acute And Chronic Hypoxic Insult, Benjamin H. Beck, S. Adam Fuller, Chao Li, Bartholomew W. Green, Honggang Zhao, Steven D. Rawles, Carl D. Webster, Eric Peatman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of fish prized for recreational angling in the United States, and there and abroad as a high-value farmed fish. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we …

Effect Of Green Tea On Interaction Of Lipid Oxidation Products With Sarcoplasmic And Myofibrillar Protein Homogenates Extracted From Bovine Top Round Muscle, Nahathai Stapornkul, Tatiana Prytkova, Lilian Were

Food Science Faculty Articles and Research

The interaction between lipid oxidation products and bovine sarcoplasmic (SP) and myofibrillar protein (MP) homogenates in the presence of green tea was investigated. To monitor the effect of green tea on lipid oxidation, aldehydes were measured while effect on protein was monitored via changes in myoglobin, thiols, and tryptophan fluorescence over nine days of refrigerated storage. The presence of SP and MP decreased free aldehydes in the buffers. The SP bound more aldehydes than MP. The tea compounds exhibited more favorable binding energies than aldehydes near histidine 64 close to the heme moiety of myoglobin. Addition of tea lowered tryptophan …

Observations On Growth And Maturation Of A Male Alloscorpiops Wongpromi (Scorpiones: Euscorpiidae), František Kovařík, Michael E. Soleglad, Graeme Lowe, Jana Plíškova, František Šťáhlavský

Euscorpius

An adult male of Alloscorpiops wongpromi Kovařík et al., 2013 was raised from birth, and its five exuviae (instars 2 through 6) were compared to the resulting adult. This adult was contrasted with the original holotype male specimen, which was determined to be only a subadult after the fifth ecdysis. The hemispermatophore of A. wongpromi is illustrated and described here for the first time. In addition to morphological analysis we also describe the karyotype of Alloscorpiops wongpromi (2n=95). Based on comparative analysis of pectinal tooth counts, it is determined that the holotype of A. troglodytes Lourenço & Pham (2015) represents …

Optimal Feeding Time And Duration Of Hybrid Crappie Kept Indoors, Wiles N. Brandon, Gregory A. Dudenhoeffer, Thomas R. Omara-Alwala

Cooperative Extension Research Publications

Crappie (Pomoxis spp.) is a popular sport fish with food fish potential. Feeding behavioral patterns should be considered prior to nutrtitional studies. The objective of this study was to determine the best feeding time and duration for hybrid crappie raised in indoor recirculating aquaculture systems. Three feeding treatments with three replciates consisted of two 12 hour feeding periods initiated at 0800 (12h-amLD) and 1700 (12h-pmLD) and a 24 feeding period started at 0800 (24h-amLD). Treatments in experimental tanks were arranged in a random block design and stocked with 14 hybrid fingerlings fed for 12 weeks. Fingerling fish were spwaned in …

Reproduction And Birth In The “Vorhiesi” Group Of The Genus Vaejovis (Scorpiones: Vaejovidae). Part I. Clutch Size, Richard F. Ayrey

Euscorpius

A total of 2,287 newborn scorpions were counted and photographed on 100 females of all 12 described species of the “vorhiesi” group of the genus Vaejovis (Scorpiones: Vaejovidae) found in Arizona, USA, and several undescribed species. Average clutch size for the “vorhiesi” group and for each individual species is reported. The data clearly shows that the larger species have more young, per parturition, than the smaller species. Representative photographs are presented for each species discussed.

Biweekly Growth Characteristics Of Juvenile Bluegill Fed Different Protein Diets, Wiles N. Brandon, Gregory A. Dudenhoeffer, Yongfang Zhang, Thomas R. Omara-Alwala

Cooperative Extension Research Publications

This study evaluated growth rates of juvenile bluegill (Lepomis macrochius) fed different protein levels, raised indoors. Six experimental diets containing protein levels of 32, 35, 38, 41, 44, and 47% with 346 kcal/g energy were fed to four replicates of 12 fish with initial mean weight of 24.9±0.51g. Fish were fed by hand to satiation three times daily for 16 weeks. Percent weight gain, for fish fed 32, 35, 38, 41, 44, and 47% protein diets were 180.5±43.8, 203.6±32.33, 256.4±19.4, 235.8±20.63, 249.1±19.59, and 275.2±47.3, and specific growth rates 0.91±0.15, 0.98±0.10, 1.13±0.05, 1.08±0.05, 1.11±0.05, and 1.17±0.11, respectively for the study. Biweekly …

The Effects Of Dietary Protein-To-Energy Ratio On Juvenile Bluegill (Lepomis Macrochirus) Growth With Observations Of Sexual Dimorphism, K. Vandeloecht, Gregory A. Dudenhoeffer, Yongfang Zhang, T.R. Omara-Alwala

Cooperative Extension Research Publications

Bluegill is a promising aquaculture species in the Midwest. males are typically larger than females. However, there is little or no information on nutritional differences between sexes and protein-to-energy ratio. The objective of this study was to characterize the protein-to-energy ratio in juvenile bluegill with observations on gender differences. Bluegill (20-30g) were fed diets consisting of 35% protein with 14, 16, and 18% lipids and 38% protein with 12, 14, and 16% lipids. Four replicates of 12 fish each were fed the diets for 16 weeks. Terminal mean body weights, lengths, liver wights, visceral weights, hepatic somatics indices (HSI), and …

The Distribution Of Α-Amylase Activity Along The Digestive Tract Of Juvenile Bluegill (Lepomis Macrochirus) And Its Comparison With Selected Fish Species, Namrata Giri, Yongfang Zhang, Gregory A. Dudenhoeffer, Thomas R. Omara-Alwala

Cooperative Extension Research Publications

Carbohydrates are the least expensive form of dietary energy for animals. Although fish don't have specific requirements for dietary carbohydrates, they must be provided with the appropriate levels of carbohydrates in their diets. Moderate levels of carbohydrate could support fishs' better growth performance and may decrease the use of the more expensive protein and lipids in the diet. Different fish have different ability to utilize digestible carbohydrates, which may relate to the relative amount of amylase activity present in the digestive system of the various species. α-amylase is a key enzyme for carbohydrate digestion. Bluegill, an important forage and recreational …

Amino Acid Profiles In The Tissues Of Juvenile Bluegill (Lepomis Macrochirus) From The Wild And Commercially-Fed Fish, Yongfang Zhang, Gregory A. Dudenhoeffer, Namrata Giri, James E. Wetzel

Cooperative Extension Research Publications

Amino acid profiles of fish tissues and fish eggs have been extensively investigated in some fish species. Amino acid profiles are helpful in quantifying the indispensable amino acid requirements for these fish. Bluegill is a major forage fish for the largemouth bass (Micropterus samoides) and an important recreational fish throughout the US. The demand for bleugill as a food fish has increased recently. However, the information on the amino acid profiles of its eggs and tissues is lacking. The objectve of this study was to determine the amino acid profiles of the muscles and whole body of the 1-2 g …

Effects Of Soybean Meal Substitution For Fish Meal In Diets Of Juvenile Bluegill (Lepomis Macrochrius), K. Stuckenschneider, A. Allen, A. Weckenborg, Gregory A. Dudenhoeffer

Cooperative Extension Research Publications

Bluegill has potential for production as a food fish. Feed is 40-60% of total production costs. Few studies have been conducted on bluegill replacing fish meal with soybean meal, which is a less expensive plant protein source. The objective of this experiment was to determine the effect of 25, 50, 75% soybean meal substitution of fish meal protein in juvenile bluegill diets. Treatments consisted of a 38% crude protein control diet, and diets with 25, 50, and 75% of fish meal replaced with soybean meal (SB Sub). All diets were insonitrogenous and isocaloric. A completely randomized block design was used …