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Prevalence And Control Of Salmonella Spp. And Sanitary Indicator Microorganisms In Wild Caught And Farm Raised Catfish (Ictalurus Punctatus), Bilan Costley Jessie Oct 2019

Prevalence And Control Of Salmonella Spp. And Sanitary Indicator Microorganisms In Wild Caught And Farm Raised Catfish (Ictalurus Punctatus), Bilan Costley Jessie

LSU Doctoral Dissertations

Catfish inspections have transitioned from the U.S. Food and Drug Administration (FDA) to the United States Department of Agriculture (USDA). The USDA Food Safety and Inspection Service (FSIS) and Food Emergency Response Network (FERN) consider it important to assess the food safety risk associated with consuming catfish in the United States. Surveillance of farm-raised and wild-caught catfish for pathogens is relevant as a public safety protocol. The purpose of this study was to detect the prevalence of Salmonella spp., identify and examine isolates for antibiotic resistance to clinical treatments, evaluate the effectiveness of antimicrobial chemical treatments against Salmonella enterica, and …


Characterizing Louisiana's Freshwater Commercial Fisheries, Lauren Bonatakis Oct 2019

Characterizing Louisiana's Freshwater Commercial Fisheries, Lauren Bonatakis

LSU Master's Theses

As an important contributor to Louisiana’s economy, the commercial freshwater fisheries have been the subject of growing attention in recent years by resource managers. Compared to the marine sector, little is known about these fisheries. Anecdotally, the fisheries appear to be on the brink of collapse. Fewer young fishermen are entering the field, fish buyers and processors are closing, and market prices remain stagnant. Because of this, the fisheries lack the resources needed to perpetuate the success and sustainability for future generations. To better understand this industry, I conducted a two-part study to characterize the commercial freshwater fisheries in Louisiana. …


Dental And Mandibular Anomalies In White-Tailed Deer (Odocoileus Virginianus) From Central Georgia, Patrick M. Powers, Alfred J. Mead Oct 2019

Dental And Mandibular Anomalies In White-Tailed Deer (Odocoileus Virginianus) From Central Georgia, Patrick M. Powers, Alfred J. Mead

Georgia Journal of Science

The frequency of dental and mandibular anomalies in free-ranging white-tailed deer in the southeastern United States is not well documented. Characteristic irregularities include supernumerary and missing teeth, malocclusion, root abscesses due to bacterial infections, and tooth or bone damage due to trauma. In the present study, we examined 778 white-tailed deer dentaries collected from the Piedmont National Wildlife Refuge in central Georgia. All dentaries were inspected for lesions, tooth irregularities, developmental anomalies, and other pathologies. Thirty-two dentaries (4.1%) displayed signs of dental or bone abnormalities. More abnormalities were associated with infection or injury (22/778, 2.8%) compared to unusual tooth development …


Future Importance Of Healthy Oceans: Ecosystem Functions And Biodiversity, Marine Pollution, Carbon Sequestration, Ecosystem Goods And Services, Mohammad Mosharraf Hossain Oct 2019

Future Importance Of Healthy Oceans: Ecosystem Functions And Biodiversity, Marine Pollution, Carbon Sequestration, Ecosystem Goods And Services, Mohammad Mosharraf Hossain

Journal of Ocean and Coastal Economics

The paper provides a review the current status of ecosystem and resource extraction to provide a series of thoughts related to the future challenges in maintaining the health of the Marine and coastal ecosystems at the Bay of Bengal. The chapter highlighted the challenges current efforts and future interventions necessary to keep the Bay of Bengal large marine ecosystem healthy. They are mainly linked to climate change, environmental pollution from different sources, biodiversity conservation, sediment movement. marine spatial planning and adoption of protected area concept to design marine reserve, fish sanctuary and ecological critical areas in the coastal zone were …


Hypoxia In Mangroves: Occurrence And Impact On Valuable Tropical Fish Habitat, Alexia Dubuc, Ronald Baker, Cyril Marchand, Nathan Waltham, Marcus Sheaves Oct 2019

Hypoxia In Mangroves: Occurrence And Impact On Valuable Tropical Fish Habitat, Alexia Dubuc, Ronald Baker, Cyril Marchand, Nathan Waltham, Marcus Sheaves

School of Marine and Environmental Sciences Faculty and Staff Publications and Presentations

Intertidal mangrove forests are harsh environments that can naturally experience hypoxia in association with low tide. However, we know relatively little about dissolved oxygen (DO) fluctuations and DO-induced responses by fish, although DO is a fundamental water quality parameter. This study examines DO as a potential factor regulating the utilization of intertidal mangrove forests by fish and consequently their widely recognized feeding, refuge and nursery values. We deployed underwater video cameras, coupled with DO and depth loggers, in a mangrove forest to record changes in fish assemblages in response to tidal variations in DO and other associated environmental parameters. Our …


Green Club After School Program Curriculum, Megan Willburn Oct 2019

Green Club After School Program Curriculum, Megan Willburn

Honors Program: Expanded Learning Clubs

Green Club is an after school program which encourages students to develop environmental empathy, passions for environmental sustainability, and knowledge about the workings of environmental systems.


Forage News [2019-10], Department Of Plant And Soil Sciences, University Of Kentucky Oct 2019

Effects Of Poor Sanitation Procedures On Cross-Contamination Of Animal Species In Ground Meat Products, Sunjung Chung, Rosalee S. Hellberg Oct 2019

Effects Of Poor Sanitation Procedures On Cross-Contamination Of Animal Species In Ground Meat Products, Sunjung Chung, Rosalee S. Hellberg

Food Science Faculty Articles and Research

The presence of <1% of an undeclared species in ground meat is generally thought to be indicative of cross-contamination as opposed to intentional mislabeling; however, this has not been experimentally tested. The objective of this study was to quantify the effects of poor sanitation on the cross-contamination of animal species in ground meat products, with the example of undeclared pork in ground beef. Cross-contamination was quantified using real-time polymerase chain reaction (PCR). Three different sanitation treatments were tested with a commercial grinder (“no cleaning”, “partial cleaning”, or “complete cleaning”) in between grinding of pork and beef samples (13.6 kg each). A 100-g sample was collected for each 0.91 kg (2 lb) of beef processed with the grinder and each sanitation treatment was tested twice. For the “no cleaning” treatment, the first 100-g sample of ground beef run through the grinder contained 24.42 ± 10.41% pork, while subsequent samples (n = 14) contained <0.2% pork. With “partial cleaning,” the first sample of ground beef contained 4.60 ± 0.3% pork and subsequent samples contained <0.2% pork. Pork was not detected in ground beef following “complete cleaning.” These results indicate that incomplete cleaning of grinding equipment leads to species cross-contamination at levels of <1% in most cases. Proper sanitation procedures must be followed when grinding multiple species in order to prevent cross-contamination and product mislabeling.


The Influence Of Location, Positioning, And Seasonality On Feeding Behavior Of The Sydney Oyster (Saccostrea Glomerata) In New South Wales, Australia, Nathaniel Hess Oct 2019

The Influence Of Location, Positioning, And Seasonality On Feeding Behavior Of The Sydney Oyster (Saccostrea Glomerata) In New South Wales, Australia, Nathaniel Hess

Independent Study Project (ISP) Collection

Oysters are hard shelled bivalves that aggregate over time to create structures in estuarine systems, known as oyster reefs. These reefs are important for the promotion of estuarine biodiversity by supporting many species of fish, invertebrates, and plants. They also act as a key contributor to water health by using active suspension feeding mechanisms and selective feeding to remove nutrients and water borne pollutants from estuarine systems. They have been touted as possible bio-remediators. They also effect rates of sedimentation in estuaries.

Oyster reefs have historically been threatened by anthropogenic influences such as overharvesting, destructive fishing practices, water pollution, CO2 …


Effect Of Dietary Replacement Of Fishmeal By Insect Meal On Growth Performance, Blood Profiles And Economics Of Growing Pigs In Kenya, Shaphan Y. Chia, Chrysantus M. Tanga, Isaac M. Osuga, Alphonce O. Alaru, David M. Mwangi, Macdonald Githinji, Sevgan Subramanian, Komi K.M. Fiaboe, Sunday Ekesi, Joop J.A. Van Loon, Marcel Dicke Oct 2019

Effect Of Dietary Replacement Of Fishmeal By Insect Meal On Growth Performance, Blood Profiles And Economics Of Growing Pigs In Kenya, Shaphan Y. Chia, Chrysantus M. Tanga, Isaac M. Osuga, Alphonce O. Alaru, David M. Mwangi, Macdonald Githinji, Sevgan Subramanian, Komi K.M. Fiaboe, Sunday Ekesi, Joop J.A. Van Loon, Marcel Dicke

All Peer-Reviewed Publications

Pig production is one of the fastest growing livestock sectors. Development of this sector is hampered by rapidly increasing costs of fishmeal (FM), which is a common protein source in animal feeds. Here, we explored the potential of substituting FM with black soldier fly larval meal (BSFLM) on growth and blood parameters of pigs as well as economic aspects. At weaning, 40 hybrid pigs, i.e., crossbreeds of purebred Large White and Landrace were randomly assigned to five iso-nitrogenous and iso-energetic dietary treatments: Control (0% BSFLM and 100% FM (T0)), and FM replaced at 25% (T25), 50% (T50), 75% (T75) and …


Rose-Ringed Parakeets, Page E. Klug, William P. Bukoski, Aaron B. Shiels, Bryan M. Kluever, Shane R. Siers Oct 2019

Rose-Ringed Parakeets, Page E. Klug, William P. Bukoski, Aaron B. Shiels, Bryan M. Kluever, Shane R. Siers

Wildlife Damage Management Technical Series

Rose-ringed parakeets (Psittacula krameri; hereafter RRPA; Figure 1) are an invasive species in the United States, present in Alabama, Louisiana, Texas, and Virginia, and with established populations in California, Florida, and Hawaii. They are also the most successful species of invasive parakeet, worldwide. RRPA can cause significant damage to agriculture, including grains, oilseeds, fruits, and ornamental plants. Large flocks of RRPA roost near human infrastructure resulting in concerns about human health and safety (e.g., collisions with aircraft, disease transmission, feces accumulation, and noise complaints). The population growth and spread of RRPA is of conservation concern given the potential impact on …


Tracking Canada Geese Near Airports: Using Spatial Data To Better Inform Management, Ryan Askren, Brett E. Dorak, Heath M. Hagy, Michael W. Eichholz, Brian E. Washburn, Michael P. Ward Oct 2019

Tracking Canada Geese Near Airports: Using Spatial Data To Better Inform Management, Ryan Askren, Brett E. Dorak, Heath M. Hagy, Michael W. Eichholz, Brian E. Washburn, Michael P. Ward

United States Department of Agriculture Wildlife Services: Staff Publications

The adaptation of birds to urban environments has created direct hazards to air transportation with the potential for catastrophic incidents. Bird–aircraft collisions involving Canada geese (Branta canadensis; goose) pose greater risks to aircraft than many bird species due to their size and flocking behavior. However, information on factors driving movements of geese near airports and within aircraft arrival/departure areas for application to management are limited. To address this need, we deployed 31 neck collar-mounted global positioning system transmitters on Canada geese near Midway International Airport in Chicago, Illinois, USA during November 2015 to February 2016. We used the movement data …


Impact Of An Educational Program On A Year-Round Forage Production And Grazing Management System In Alabama, Lila Karki, Uma Karki Oct 2019

Impact Of An Educational Program On A Year-Round Forage Production And Grazing Management System In Alabama, Lila Karki, Uma Karki

Professional Agricultural Workers Journal

Abstract

Raising animals on supplementary feeds in the lean months is economically unfeasible due to the increasingly high price of grains and commercial feeds. The objective of this study was to estimate the economic impact of educational events on year-round pasture and grazing management. A survey was introduced through SurveyMonkey to 78 trainees to collect data on a pre-structured questionnaire. A conceptual framework of production function was applied to measure the impact of the events using a before vs. after impact assessment approach. Forty-six percent of respondents completed the survey. The results revealed that the educational events had a positive …


Barriers To Management Intensive Grazing By Southern Dairy Farmers, Mohammed Ibrahim, Nalini K. Pattanaik, Brian Cornish Oct 2019

Barriers To Management Intensive Grazing By Southern Dairy Farmers, Mohammed Ibrahim, Nalini K. Pattanaik, Brian Cornish

Professional Agricultural Workers Journal

Abstract

Interest in Management Intensive Grazing (MIG) [a situation where grazing animals are moved to a fresh pasture every few days in order to have access to adequate forage] practices by farmers have increased steadily over the years. Many research publications on grazing advocate the financial and environmental benefits of grazing. Understanding the challenges of MIG can be an important piece of information for a dairy farmer. A survey was conducted to determine how farmers in the southeastern region perceive the barriers to the adoption of MIG. A greater percentage of MIG southeastern farmers were satisfied or very satisfied with …


Production Period Of Different Browse Species Suitable For Grazing Small Ruminants, Bidur Paneru, Uma Karki, Shailes Bhattrai, Nevershi Ellis Oct 2019

Production Period Of Different Browse Species Suitable For Grazing Small Ruminants, Bidur Paneru, Uma Karki, Shailes Bhattrai, Nevershi Ellis

Professional Agricultural Workers Journal

Abstract

Browse (shrubs, trees, and vines) species are important sources of feed for small ruminants in Alabama and many other states of the U.S. Information on the production period of these species is important to prepare a year-round grazing plan to utilize them well. The objective of the study was to determine the leaf-shedding time of common browse species suitable for small ruminants. This study was conducted at the Atkins Agroforestry and Browse Research and Demonstration Site of Tuskegee University, Alabama. Ten samples of each browse species were tagged, and leaf-yellowing and leaf-fall data collected using photoplots every 14 days …


Economic And Livestock Health Impacts Of Birds On Dairies: Evidence From A Survey Of Washington Dairy Operators, Julie L. Elser, Amber L. Adams Progar, Karen M.M. Steensma, Tyler P. Caskin, Susan R. Kerr, Stephanie A. Shwiff Sep 2019

Economic And Livestock Health Impacts Of Birds On Dairies: Evidence From A Survey Of Washington Dairy Operators, Julie L. Elser, Amber L. Adams Progar, Karen M.M. Steensma, Tyler P. Caskin, Susan R. Kerr, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

The survey described in this research paper aimed to investigate the economic and health impacts of birds on dairies. Birds are common pests on dairies, consuming and contaminating feed intended for cattle. As a result, dairy operators experience increased feed costs and increased pathogen and disease risk. We surveyed dairy operators attending the 2017 Washington Dairy Conference to examine the impact of birds on dairies in Washington State. Dairy operators reported feed losses valued at $55 per cow resulting in annual losses totaling $5.5 million in the Western region of the state and $9.2 million in the Eastern region of …


Use Of Probiotic Bacterial Strains And Cell Extracts To Inhibit Acidosis And Liver Abscesses In Cattle, Samodha Charaka Fernand Sep 2019

Use Of Probiotic Bacterial Strains And Cell Extracts To Inhibit Acidosis And Liver Abscesses In Cattle, Samodha Charaka Fernand

Department of Animal Science: Faculty Publications

Disclosed are methods of using probiotic bacterial strains Aneurinibacillus migulanus, Aneurinibacillus aneurinilyti cus and / or extracts thereof for inhibiting the growth of Streptococcus bovis , Fusobacterium necrophorum , and Acranobacterium ( Actinomyces ) pyogenes . These bacterial strains and / or their extracts can be used in cattle to treat acidosis and / or liver abscesses , as well as inhibit foot rot, among other infections and conditions caused by Strepto coccus bovis and / or Fusobacterium necrophorum and / or Acranobacterium (Actinomyces) pyogenes. Compositions for use in these methods are also provided.


Predicting Functional Responses In Agro-Ecosystems From Animal Movement Data To Improve Management Of Invasive Pests, Mark Q. Wilber, Sarah M. Chinn, James C. Beasley, Raoul K. Bourghton, Ryan K. Brooks, Stephen S. Ditchkoff, Justin W. Fischer, Steve B. Hartley, Lindsey K. Holmstrom, John C. Kilgo, Jesse S. Lewis, Ryan S. Miller, Nathan P. Snow, Kurt C. Vercauteren, Samantha M. Wisely, Colleen T. Webb, Kim M. Peen Sep 2019

Predicting Functional Responses In Agro-Ecosystems From Animal Movement Data To Improve Management Of Invasive Pests, Mark Q. Wilber, Sarah M. Chinn, James C. Beasley, Raoul K. Bourghton, Ryan K. Brooks, Stephen S. Ditchkoff, Justin W. Fischer, Steve B. Hartley, Lindsey K. Holmstrom, John C. Kilgo, Jesse S. Lewis, Ryan S. Miller, Nathan P. Snow, Kurt C. Vercauteren, Samantha M. Wisely, Colleen T. Webb, Kim M. Peen

United States Department of Agriculture Wildlife Services: Staff Publications

Functional responses describe how changing resource availability affects consumer resource use, thus providing a mechanistic approach to prediction of the invasibility and potential damage of invasive alien species (IAS). However, functional responses can be context dependent, varying with resource characteristics and availability, consumer attributes, and environmental variables. Identifying context dependencies can allow invasion and damage risk to be predicted across different ecoregions. Understanding how ecological factors shape the functional response in agro-ecosystems can improve predictions of hotspots of highest impact and inform strategies to mitigate damage across locations with varying crop types and availability. We linked heterogeneous movement data across …


Evaluation Of Chilling Efficiency, Meat Tenderness, And Microbial Analysis Of Broiler Carcasses Using Sub-Zero Saline Solutions, Samira Viliani Sep 2019

Evaluation Of Chilling Efficiency, Meat Tenderness, And Microbial Analysis Of Broiler Carcasses Using Sub-Zero Saline Solutions, Samira Viliani

Master's Theses

The poultry industry is seeking an advanced chilling system that can improve chilling efficiency, microbial safety, and water consumption without compromising meat quality. The objective of this study was to evaluate the effects of sub-zero saline chilling methods on chilling efficiency, breast fillet tenderness and microbial reduction of broiler carcasses. Following evisceration and rinsing, broiler carcasses were randomly assigned to one of three chilling solutions: 1) 0% salt or ice water control (0% NaCl/0.5oC), 2) 3% salt (3% NaCl/-1.8oC), and 3) 4% salt (4% NaCl/-2.41oC) solutions. Broiler carcasses in sub-zero saline solutions reached the …


Brangus Cows Have Ovarian Reserve Parameters More Like Brahman Than Angus Cows, Robert A. Cushman, Émerson M. Soares, Hannah K. Yake, Allyson L. Patterson, Shelby L. Rosasco, Joslyn K. Beard, Emmalee J. Northrop, Jerica J.J. Rich, Jeremy R. Miles, Chadwick C. Chase Jr., Michael G. Gonda, George A. Perry, Anthony K. Mcneel, Adam F. Summers Aug 2019

Brangus Cows Have Ovarian Reserve Parameters More Like Brahman Than Angus Cows, Robert A. Cushman, Émerson M. Soares, Hannah K. Yake, Allyson L. Patterson, Shelby L. Rosasco, Joslyn K. Beard, Emmalee J. Northrop, Jerica J.J. Rich, Jeremy R. Miles, Chadwick C. Chase Jr., Michael G. Gonda, George A. Perry, Anthony K. Mcneel, Adam F. Summers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Bos indicus females have more surface antral follicles than Bos taurus females; however, histological studies demonstrated no difference in total number of primordial follicles between these two biological types of cattle. Primordial follicle density in the ovary was less in Nelore ovaries compared to Angus ovaries, but no studies have examined the primordial follicle density in Bos indicus cross-bred females. It, therefore, was hypothesized that primordial follicle density in the ovary would decrease as percentage Bos indicus increased. Ovaries were collected from cross-bred Angus (n=32, no Bos indicus influence), Brangus (n=15), or Brahman (n=9) …


Comparison Of The Efficacy Of Four Drug Combinations For Immobilization Of Wild Pigs, Christine K. Ellis, Morgan E. Wehtje, Lisa L. Wolfe, Peregrine L. Wolff, Clayton D. Hilton, Mark C. Fisher, Shari Green, Michael P. Glow, Joeseph M. Halseth, Michael J. Lavelle, Nathan P. Snow, Eric H. Vannatta, Jack C. Rhyan, Kurt C. Vercauteren, William R. Lance, Pauline Nol Aug 2019

Comparison Of The Efficacy Of Four Drug Combinations For Immobilization Of Wild Pigs, Christine K. Ellis, Morgan E. Wehtje, Lisa L. Wolfe, Peregrine L. Wolff, Clayton D. Hilton, Mark C. Fisher, Shari Green, Michael P. Glow, Joeseph M. Halseth, Michael J. Lavelle, Nathan P. Snow, Eric H. Vannatta, Jack C. Rhyan, Kurt C. Vercauteren, William R. Lance, Pauline Nol

United States Department of Agriculture Wildlife Services: Staff Publications

Field immobilization of native or invasive wild pigs (Sus scrofa) is challenging. Drug combinations commonly used often result in unsatisfactory immobilization, poor recovery, and adverse side effects, leading to unsafe handling conditions for both animals and humans. We compared four chemical immobilization combinations, medetomidine–midazolam–butorphanol (MMB), butorphanol–azaperone–medetomidine (BAM™), nalbuphine–medetomidine–azaperone (NalMed-A), and tiletamine– zolazepam–xylazine (TZX), to determine which drug combinations might provide better chemical immobilization of wild pigs. We achieved adequate immobilization with no post-recovery morbidity withMMB. Adequate immobilization was achieved with BAM™; however, we observed post-recovery morbidity. Both MMB and BAM™ produced more optimal results relative to body temperature, recovery, and …


Negative Impacts Of The Beef Industry: Lab-Grown Meat, Stephanie Grass Aug 2019

Negative Impacts Of The Beef Industry: Lab-Grown Meat, Stephanie Grass

WRIT: Journal of First-Year Writing

The beef industry is harmful to the environment and human health and alternative solutions must be implemented in order to mitigate the effects of climate change. Water and grain are used in agriculture in abundance despite the negative environmental effects it causes. Cattle are the biggest contributors to greenhouse gas emissions in the sector, also contributing to climate change. Antibiotics are used in large quantities without regard to potential future consequences. One potential solution for this problem is lab-grown beef, which demands very little from the consumer and would take pressure off the environmental issues the beef industry creates. Lab-grown …


Effects Of Adrenergic Or A Combination Of Adrenergic And Opioid Drugs On Assessments Of Insulin Sensitivity In Mares, Lauren Kerrigan Aug 2019

Effects Of Adrenergic Or A Combination Of Adrenergic And Opioid Drugs On Assessments Of Insulin Sensitivity In Mares, Lauren Kerrigan

LSU Master's Theses

Excitement is a problem when conducting endocrine tests on fractious horses. Epinephrine (EPI), for example, was shown to obliterate the insulin-induced decrease in blood glucose concentrations. Sedation may be a solution; however, perturbation of results may preclude useful information. The objectives of the four experiments presented herein were 1) to determine the effects of EPI on insulin response to glucose infusion (IR2G), 2) to assess the effects of the α-adrenergic sedative, detomidine (DET), alone or in combination with the opioid agonist, butorphanol (BUT), on IR2G and glucose response to insulin (GR2I), and 3) to assess the effects of BUT alone …


An Epidemiological Study Of Diabetes Mellitus In Dogs Attending First Opinion Practice In The Uk, Madeleine Mattin, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt Aug 2019

An Epidemiological Study Of Diabetes Mellitus In Dogs Attending First Opinion Practice In The Uk, Madeleine Mattin, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt

Epidemiology Collection

Objectives: To estimate the prevalence of canine diabetes mellitus (DM) in primarycare clinics in England, to identify risk factors associated with DM and to describe the survival of affected dogs.

Methods: Cases of DM were identified within the electronic patient records of 89 small-animal practices. A nested case-control study identified risk factors for the diagnosis of DM using logistic regression models. Cox proportional hazards models were used to analyse variables associated with survival.

Results: Four-hundred and thirty-nine canine DM cases were identified, giving an apparent prevalence of 0.34% (95% confidence interval 0.31 - 0.37%). Neutered males were at an increased …


Incidence And Prevalence Of Francisella Noatunensis Subsp. Orientalis (Fno) Pathogenic Infections In Feral Hawaiian Tilapia, Daquille Peppers Aug 2019

Incidence And Prevalence Of Francisella Noatunensis Subsp. Orientalis (Fno) Pathogenic Infections In Feral Hawaiian Tilapia, Daquille Peppers

Biology Theses

Aquaculture has become a major food source for many countries and continues to grow each year. However, many of the fishes that are farmed are susceptible to pathogenic infections such as Francisellosis, a bacterial infection that contributes to disease and high mortality rate in many populations of farmed and wild fish around the world. In 1994, feral and farmed populations of tilapia (e.g. Oreochromis mossambicus) on Oahu, Hawaii were found to be infected with a novel Francisellosis strain, Francisella noatunensis subsp. orientalis (Fno). To prevent the spread of this pathogen, the Hawaii Department of Aquaculture (HDOA) initiated …


Investigation Of Microbiota In Health And Disease Of Poultry, Bishnu Adhikari Aug 2019

Investigation Of Microbiota In Health And Disease Of Poultry, Bishnu Adhikari

Graduate Theses and Dissertations

The microbiotas play vital roles in health and diseases of both humans and animals. 16S rRNA genes sequence analysis is one of the most popular and commonly used methods in the analysis of microbiotas associated with hosts. In this dissertation, the microbiotas of chickens (broilers, breeders, and layers) and turkeys were evaluated by 16S rRNA gene sequencing. Characterization of the culturable subpopulations of Lactobacillus in the chicken gut can serve as a valuable resource for probiotic development. In Chapter 2, Lactobacillus subpopulations recovered on MRS from chicken gut were defined comprehensively for the first time using 16S rRNA gene profiling, …


Characterization Of The Swine Gut Microbiome Dynamics, Culturomics And Probiotics, Xiaofan Wang Aug 2019

Characterization Of The Swine Gut Microbiome Dynamics, Culturomics And Probiotics, Xiaofan Wang

Graduate Theses and Dissertations

The gastrointestinal tract is a critical place where the immune system concentrates many of its protective functions. It exerts both physical and biochemical defenses against invading foodborne pathogens and other hazards. In pigs, the gut microbiota community assembled postnatal plays a very important role in the development of multiple physiologic aspects, such as development of the gastrointestinal tract, nutrient exchange, immune defense against foreign pathogens and growth performance. Microbiome-host communication changes with age. Despite recent advances in understanding the swine gut microbiome at different growth stages, a comprehensive longitudinal study of the lifelong dynamics of the swine gut microbiome is …


Effects Of Late Gestation Supplementation And Creep Feeding On Spring Calving Beef Cows In The Nebraska Sandhills, Devin Lynn Broadhead Aug 2019

Effects Of Late Gestation Supplementation And Creep Feeding On Spring Calving Beef Cows In The Nebraska Sandhills, Devin Lynn Broadhead

Department of Animal Science: Dissertations, Theses, and Student Research

The objectives of this research were to 1) evaluate the effects of late gestation supplementation, synchronization and creep feeding on cow and calf production traits 2) evaluate the pooled effects of 5 studies involving late gestation supplementation on cow and calf traits. Experiment 1 was a three year study conducted on 8 pastures at the Gudmundsen Sandhills Laboratory. Cows were assigned to 1 of 4 late-gestation supplementation treatments, postpartum progestin or control, and 1 of 2 creep feed treatments to make up a 4 x 2 x 2 factorial arrangement. Calves were followed through to slaughter.

Experiment 2 involved 5 …


The Effect Of Lactation And Energy Status On Gene Expression In The Main Reproductive Tissues Of Lactating Dairy Cattle, Sameer M. Alhojaily Aug 2019

The Effect Of Lactation And Energy Status On Gene Expression In The Main Reproductive Tissues Of Lactating Dairy Cattle, Sameer M. Alhojaily

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Modern high-yielding dairy cows are currently producing far more milk than their ancestors due to a prolonged and intensive genetic selection for milk production trait accompanied by the revolutionary improvement in technology, management, and nutrition. On the other hand, a noticeable decline in fertility and reproductive performance was undeniably consistent with the increase in milk yield. This decline in fertility and reproductive performance are recognized worldwide and well documented in several studies. Dairy cows typically experience a period of energy deficit during the first few months of lactation due to the rapid increase in milk production and limited feed intake. …


Effects Of Alternative Carbohydrate Sources And Soybean-Derived Isoflavones On The Microbiome And Immune System Of Nursery Piglets And Intestinal Porcine Epithelial Cell Line J-2, Sydney Kinstler Aug 2019

Effects Of Alternative Carbohydrate Sources And Soybean-Derived Isoflavones On The Microbiome And Immune System Of Nursery Piglets And Intestinal Porcine Epithelial Cell Line J-2, Sydney Kinstler

Department of Animal Science: Dissertations, Theses, and Student Research

Four experiments were conducted to characterize the microbiome of the piglet and observe effects of diet on growth performance and immunity. In Exp. 1, 96 nursery piglets were fed varying carbohydrate sources and in Exp. 2, 64 piglets were fed a soy protein concentrate or soybean meal as-is or supplemented with an isoflavone concentrate. Body and feeder weights were measured for growth performance estimation. Luminal contents and scrapings were isolated for microbial composition analysis. Fecal and blood samples were extracted in Exp. 2 for microbiome and immunity analysis. Diets did not affect growth performance (P > 0.10); however, Exp. 1 …