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Genetic Parameter Estimates Of Fear, Growth, And Carcass Characteristics In Japanese Quail, Doğan Nari̇nç, Bariş Aybars Genç

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to estimate the heritability values of tonic immobility duration, several growth traits, and their genetic relationships with some slaughter traits. Fearfulness reaction was examined using tonic immobility (TI) response at 5 and 6 weeks of age. All birds were slaughtered at 6 weeks of age to measure carcass yield (CY), and percentage of breast (BY), leg (LY), wing (WY), abdominal fat (AFY). Heritability estimates for the TI durations and body weights measured at 5 and 6 weeks of age were 0.16, 0.20, and 0.46, 0.44, respectively. Heritability estimates for growth curve parameters and inflection …

Effects Of Dietary Guar Meal With Or Without Beta-Mannanase On The Egg Yolk Fatty Acids,Cholesterol, And Some Blood Parameters Of Laying Hens, Süleyman Çalişlar

Turkish Journal of Veterinary & Animal Sciences

In this study, the effects of adding enzyme to the diets containing guar meal (GM) on the egg yolk fatty acids, cholesterol, and some blood plasma parameters of hens were examined. This study was carried out in the chicken coop of the Experimental Animals Ethics Committee, Faculty of Agriculture. This study constitutes the second phase of the study titled ?Effects of dietary guar meal with or without beta-mannanase on performance and egg quality traits in laying hens?, previously published. The experimental period lasted 126 days. Ninety-six Lohman Brown hens at the age of 56 weeks were and kept in individual …

Pharmacokinetics And Reward-Related Behaviors Of Ethanol In Male And Female Japanese Quail (Coturnix Japonica), Shannon Eaton

Theses and Dissertations--Psychology

Ethanol is one of the most widely used and abused drugs. Problem use is associated with many different health problems and the economic burden is in the billions of dollars. Additionally, many people have difficulty controlling their ethanol consumption and about 5% of adults end up with an alcohol use disorder (AUD). Many people with an AUD often find themselves in a cycle of binge, remission, and relapse.

Following ethanol consumption ethanol enters the bloodstream from the small intestine where it gets distributed to peripheral tissues. Ethanol in the bloodstream is cleared from the system by the liver. The primary …

Small Unmanned Aircraft Systems Acoustic Analysis For Noninvasive Marine Mammal Response: An Exploratory Field Study, David Thirtyacre, Gennifer Brookshire, Sarah Callan, Brittany Arvizu, Patrick Sherman

International Journal of Aviation, Aeronautics, and Aerospace

As in countless other fields of human endeavor, small unmanned aircraft systems (sUAS) have the potential to benefit pinniped (Pinnipedia; e.g., Phocidae [seals], Otariidae [sea lions], and Odobenidae [walruses]) response efforts. The employment of sUAS could give responders a close-up look at animals in distress in order to determine their condition as well as develop a response strategy. However, unlike other subjects that are regularly inspected by sUAS (e.g., croplands and civil infrastructure) pinnipeds may respond to the distinctive sound generated by small, multirotor sUAS. This reaction may include retreating into the water en masse, which could put …

Salad With Clams: Prey Choice Of An Intentionally Carnivorous Turtle, Kayleigh R. Erazmus, Luca Luiselli, Russell L. Burke

Biology Faculty Publications

Prey choice is the non-random foraging and consumption of prey species by their predators, and is therefore the basis for studies of topics as diverse as quantifying food webs, predator–prey relationships, and optimal-foraging models. Malaclemys terrapin (Diamond-back Terrapin) is a diet generalist with a large geographic distribution: the US Atlantic and Gulf coasts from Massachusetts to Texas. Individual terrapins have relatively small home ranges and feed primarily on local mollusc species. In feeding trials with 1 prey species and 2 prey species, wild-caught terrapins from New York readily consumed Mya arenaria (Soft-shelled Clam) and Geukensia demissa (Atlantic Ribbed Mussel), preferring …

Flight And Dietary Antioxidants Influence Antioxidant Expression And Activity In A Migratory Bird, Kristen J. Demoranville, Wales A. Carter, Barbara J. Pierce, Scott R. Mcwilliams

Biology Faculty Publications

Ecologically-relevant factors such as exercise and diet quality can directly influence how physiological systems work including those involved in maintaining oxidative balance; however, to our knowledge, no studies to date have focused on how such factors directly affect expression of key components of the endogenous antioxidant system (i.e., transcription factors, select antioxidant genes, and corresponding antioxidant enzymes) in several metabolically active tissues of a migratory songbird. We conducted a 3-factor experiment that tested the following hypotheses: (H1) Daily flying over several weeks increases the expression of transcription factors NRF2 and PPARs as well as endogenous antioxidant genes (i.e., CAT, SOD1, …

Status Reports Of The Fisheries And Aquatic Resources Of Western Australia 2019/20. State Of The Fisheries, Daniel J. Gaughan, K. Santoro

Status Reports of the Fisheries and Aquatic Resources

The Status Reports of the Fisheries and Aquatic Resources of Western Australia (SRFAR) provide an annual update on the state of the fish stocks and other aquatic resources of Western Australia (WA). These reports outline the most recent assessments of the cumulative risk status for each of the aquatic resources (assets) within WA’s six Bioregions using an Ecosystem Based Fisheries Management (EBFM) approach.

Status Reports Of The Fisheries And Aquatic Resources Of Western Australia 2020/21, S.J. Newman, B.S. Wise, K.G. Santoro, D.J. Gaughan

Status Reports of the Fisheries and Aquatic Resources

The Status Reports of the Fisheries and Aquatic Resources of Western Australia (SRFAR) provide an annual update on the state of the fish stocks and other aquatic resources of Western Australia (WA). These reports outline the most recent assessments of the cumulative risk status for each of the aquatic resources (assets) within WA’s six Bioregions using an Ecosystem Based Fisheries Management (EBFM) approach. The Departments’ risk based EBFM framework is the State government’s basis for management of all Western Australia’s aquatic resources.

Use Of Redwood Basal Hollows By Bats: A Focus On The Townsend's Big-Eared Bat On The North Coast Of California, Amon Jotesh Armstrong

Cal Poly Humboldt theses and projects

Loss of roosting resources, either through disturbance or removal, negatively affects bats. For sensitive species, such as the Townsend’s big-eared bat (Corynorhinus townsendii), determining roost requirements is a critical component in conserving their habitat. Such cavity roosting bats on the North Coast of California may use hollows in large redwood trees. In this study, I examined the factors determining the use of basal tree hollows by different bat species at eight redwood forest sites in Del Norte, Humboldt, and Mendocino Counties, California. Bat guano was collected from 179 basal hollow roosts from 2017 to 2018, and guano mass …

A New Species Of Calomyscus Thomas, 1905 (Calomyscidae: Rodentia) From Western Iran, Mehdi Dezhman, Safie Akbarirad, Mansour Aliabadian, Roohollah Siahsarvie, Arya Shafaeipour, Omid Mirshamsi

Turkish Journal of Zoology

Eight extant species have been so far recognized for Calomyscus Thomas, 1905 which is distributed in the west of Asia. The only species which was reported from the Zagros Mountains in western and southern Iran was C. bailwardi. However, based on the integrative methods such as molecular, morphometric and karyological studies a new species is worth to be described other than C. bailwardi from the Zagros Mountains. In the present survey a new species which has been distributed in western regions of Iran was described as Calomyscus behzadi sp. nov. The new species constituted a monophyletic clade in phylogenetic tree …

Effects Of Using Processed Barley And Supplemented Multi-Enzymes In Laying Hen Rations On Egg Production, Egg Quality And Egg Fatty Acids, Yavuz Gürbüz, Osman Özyürür

Turkish Journal of Veterinary & Animal Sciences

In this study, we have observed different technological processes, which are commonly used in poultry production. Rations contained different amounts of barley and multi-enzyme, egg weight, egg mass, egg yield, feed intake, egg weight. The rations of the control groups were as follows: 0% barley (based of corn) (K), 15% untreated barley (A1), 15% pellet barley (A2), 15% flaked barley (A3), 30% untreated barley (A4), 30% pelleted barley (A5), 30% flaked barley (A6), 30% untreated barley + enzyme (0. 025%) (A7). In the research, 64 brown laying hens ATAK-S for 36 weeks were divided into 8 different treatments for egg …

The Effects Of The L. Plantarum Strain Rc1.4 Starter Culture With Or Without Sucrose Addition On Fermentation Efficacy, Microbial Content, And Aerobic Stability Indicators Of Alfalfa Silage, Taylan Aksu, Shami̇l Vali̇dov, Li̇li̇i̇a Kari̇mova, Murat Demi̇rel, Taner Levendoğlu, Si̇bel Erdoğan, Mehtap Güney

Turkish Journal of Veterinary & Animal Sciences

To develop the approach for efficient alfalfa silage fermentation, L. plantarum strain RC1.4 (LP-RC1.4) was used as starter culture with or without sucrose. Trial groups consisted of ten repetitions for each treatment including (1) control, with added sterile water (10 mL); (2) LP, silages treated with LP-RC1.4 alone (106 cfu g$^{-1}$ FM); (3) LP-S1, silages treated with LP-RC1.4 (10$^{6}$ cfu g$^{-1}$ FM) plus sucrose at 10 g/kg fresh matter; (4) LP-S$_{2}$, silages treated with LP-RC1.4 (106 cfu g-1 FM) plus sucrose at 20 g kg$^{-1}$ fresh matter. Inoculation improved the quality of the silaging demonstrating a lower decrease of silage …

In Ovo Feeding Technology: Embryonic Development, Hatchability And Hatching Quality Ofbroiler Chicks, Brian Tainika, Özer Hakan Bayraktar

Turkish Journal of Veterinary & Animal Sciences

This paper reviewed the effects of in ovo feeding application on incubation performance and chick quality in broilers. Effectiveness of incubation and chick quality in commercial chicken broiler production have a decisive role in production cost as well as efficiency and are a prerequisite for sustainable production. To date, several studies have been done to improve incubation performance and chick quality, and significant gains have been achieved in this area in the last fifty years. The average hatchability and survivability in the sector have reached 85%-90% and 90%-95% respectively, and with new research, theoretical limits have been closely approached. In …

The Relation Of Fat Tissue Hormones And Some Galactopoietic Hormones With Milk Yield In Holstein And Simmental Cows, Zennure Demi̇r, Devri̇m Saripinar Aksu

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to investigate the physiological changes in leptin, adiponectin, and ghrelin hormones, which are effective in the regulation of energy metabolism, and the galactopoietic hormones including growth hormone (GH), insulin, insulin-like growth factor-I (IGF-I), cortisol, thyroid stimulating hormone (TSH) and thyroxine (T4 ) levels, which are also effective in the regulation of metabolic activities and their correlation with milk yield levels in Holstein and Simmental cows with low, medium, and high milk yield. Holstein (n = 30) and Simmental cows (n = 30) with low, medium, and high milk yield were used. Cows were divided into …

Evaluating The Effects Of Different Silage Additives On Silage Quality And In Vitro Digestionvalues Of The Silages Of Leguminous And Gramineous Forage Plants Grown Withoutfertilizer And Irrigation In Central Anatolian Arid Conditions, Kami̇l Kara, Kanber Kara, Taşkin Erol, Gökhan Şen, Mehmet Aki̇f Karsli

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determine the effects of molasses, and bacterial inoculants on silage quality, fermentation characteristics, nutrient contents, and in vitro digestibility of different forage species grown at terrestrial climate of Central Anatolia without any artificial fertilizer usage and irrigation. Forage peas (Pisum arvense L.), Hungarian vetch (Vicia pannonica Crantz), rye grass (Lolium multiflorum Lam.) and triticale (xTriticosecale Wittmack) harvested at the dough stage of triticale and conserved in 1.5 kg jars. Silages were treated with no additive (control silage), 5% molasses and 10 g/t bacterial inoculant. Sensory, pH, organic acid, chemical analyses and in vitro …

Insectum Non Grata: The Harlequin Ladybird, Harmonia Axyridis (Pallas, 1773)(Coleoptera, Coccinellidae) In Turkey, Ahmet Karataş, Ayşegül Karataş, Ni̇zametti̇n Yavuz, Eli̇f Deni̇z Ülker, Özgür Koçak, Burak Akbaba

Turkish Journal of Zoology

The Harlequin Ladybird, Harmonia axyridis (Pallas, 1773), is native to Eastern Asia but it has been introduced to many countries in Africa, America, and Eurasia. In Turkey, H. axyridis is one of the most fast-spreading invasive alien species. Since the collection of the first specimens in 2010, H. axyridis spread almost all over the country. The species spreads fast in Turkey according to the new records, obtained originally from the field studies and the citizen science data. It was recorded for the first time from 19 of 81 Turkish provinces (Ankara, Antalya, Balıkesir, Bolu, Bursa, İstanbul, İzmir, Karaman, Kastamonu, Kırklareli, …

Rediscovery Of Pearly Tree Frog, Nyctixalus Margaritifer Boulenger, 1882 (Amphibia: Rhacophoridae) From Mt. Wilis After 135 Years, Bagus Priambodo, Richo Firmansyah, Dicky Candra Pranata, Afina Nur Aninnas, Muhamad Azmi Dwi Susanto, Fajar Dwi Nur Aji, Tri Wahyu Widodo, Gunawan Gunawan, Danafia Permana, Faisal Yanuar Adiba, Yuri Ristanto, Muhamad Prayogi Erfanda, Ahmad Muammar Kadafi

Turkish Journal of Zoology

For more than a century, pearly tree frog from its type locality (East Indies; Java; Mt. Wilis) are undiscovered. Current studies about the distribution of Nyctixalus margaritifer are reported as known only from West Java and Central Java. In this paper, we collected one individual of pearly tree frog, N. margaritifer in the Western part of Mt. Wilis, which belongs to Gunung Sigogor Nature Reserve, Ponorogo, East Java. This is the third specimen, which is discovered from the Mt. Wilis after the lost holotype in 1882 and the neotype in 1885. Poorly known on its ecology and its habitat preferences …

The Determination Some Biological Parameters Of Phenacoccus Madeirensis Green (Hemiptera: Pseudococcidae)) On Vegetable Plants, Mustafa Gökhan Bi̇lgi̇n, Asi̇me Fi̇li̇z Çalişkan Keçe, Mehmet Ri̇fat Ulusoy

Turkish Journal of Zoology

This study has been conducted to determine some biological parameters of the Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae) on four vegetable plants (7 different varieties); tomato (Hazera and Torry) ( Solanum lycopersicum L.), eggplant (Adana Topağı and Anamur) ( Solanum melongena L.), pepper (Safran and Zafer) ( Capsicum annuum L.), and Pepino (Minski) ( Solanum muricatum L.). Experiments were carried out in climate cabinets at 25 ± 2 °C, 60 ± 10% R.H., and 16L: 8D) in Plant Protection Department Nedim UYGUN Biological Control Laboratory of Agriculture Faculty, Çukurova University between 2017 and 2018. Life table data were analyzed by using …

The Morphometric And Erythrometric Analyses Of Pelophylax Ridibundus Living In Anthropogenic Pollution Resources, Mesut Dönmez, Turgay Şi̇şman

Turkish Journal of Zoology

The health status of Pelophylax ridibundus species living in anthropogenic pollution sources in Erzurum (Turkey) were investigated. Firstly, heavy metal concentrations in liver of the frog species, and in water and sediment samples taken from the habitats of the frogs were determined by instrumental analysis. Secondly, morphometric (fluctuating asymmetry-FA, hepatosomatic index- HSI) and erythrometric analyses were performed to reveal the health status of the frogs in the areas. Our study provides the first morphometric datas on the frog in Turkey. Although the metal concentrations in the water were high, it was determined that they did not exceed the limit values. …

Physiological Impacts Of Lampricides On Invasive Sea Lamprey (Petromyzon Marinus) And Non-Target Fishes, Razvan, Adrian Ionescu

Theses and Dissertations (Comprehensive)

Parasitic sea lamprey (Petromyzon marinus) ingest large quantities of blood from fishes using their oral disc and rasping tongue, most often killing the host. In the early 1900s, sea lamprey invaded the Laurentian Great Lakes, decimating sport, commercial and culturally significant fisheries. Since the early 1960s, chemical control using the lampricides 3- trifluoromethyl-4-nitrophenol (TFM) and niclosamide have helped to reduce sea lamprey populations by 90%. Lampricides are applied to larval lamprey nursery streams targeting many generations of lamprey at once. However, there is concern about the potential adverse effects of lampricides on other fishes, particularly vulnerable lake sturgeon (Acipenser fulvescens) …

Habitat Use Of Wintering Henslow's Sparrows (Centronyx Henslowii) In Power Line Right-Of-Ways, Abigail W. Dwire

College of Graduate Studies: Theses & Dissertations

Habitat loss and degradation are the leading causes of grassland bird declines worldwide. The Henslow’s Sparrow (Centronyx henslowii; hereafter HESP) is a grassland bird species of conservation concern that has historically relied on the herbaceous ground-layer of longleaf pine savannas and similar habitats in the southeastern U.S. for food and shelter in the winter. However, due to human development, alterations of habitat, and fire suppression, only fragments of these habitats remain. Over the last decade, surveys have annually documented HESPs using power line right-of-ways (hereafter, ROWs) at several sites in the coastal plain of Georgia as alternative habitat for …

Effects Of Large Wood Restoration On Coho Salmon In A Northern California Watershed: A Before-After-Control-Impact Experiment, Natalie B. Okun

Cal Poly Humboldt theses and projects

Substantial time, money, and effort are invested in river and stream restoration projects to aid in the recovery of imperiled salmonid populations, but there is little evidence that these efforts have had lasting positive impacts on juvenile fish growth and survival. To assess the effectiveness of large woody debris (LWD) restoration, which is one of the most common restoration practices, I evaluated the growth and survival response of endangered Central California Coast coho salmon (Oncorhynchus kisutch) in a paired watershed before-after impact-control (BACI) study. To determine if LWD supplementation influenced coho salmon growth and survival, two neighboring, similar …

Animal Sentience: History, Science, And Politics, Andrew N. Rowan, Joyce M. D'Silva, Ian J.H. Duncan, Nicholas Palmer

Animal Sentience

This target article has three parts. The first briefly reviews the thinking about nonhuman animals’ sentience in the Western canon: what we might know about their capacity for feeling, leading up to Bentham’s famous question “can they suffer?” The second part sketches the modern development of animal welfare science and the role that animal-sentience considerations have played therein. The third part describes the launching, by Compassion in World Farming, of efforts to incorporate animal sentience language into public policy and regulations concerning human treatment of animals.

Assessing Habitat Suitability Of Ribbed Mussels (Geukensia Demissa) In Georgia Salt Marshes By Examining Predicted Mussel Densities And Mussel Population Parameters, William K. Annis Jr

College of Graduate Studies: Theses & Dissertations

Geukensia demissa (ribbed mussel) is an ecology important bivalve that has the potential to contribute to salt marsh restoration. Understanding the factors that contribute to the distribution of mussels can help inform managers on choosing locations to optimize the survivorship of mussels in restoration projects. This study sought to model mussel densities across the coast of Georgia and to compare predicted mussel densities with mussel population parameters as means to gauge habitat suitability. Mussel densities were collected through field surveys across a range of salt marshes along the coast of Georgia and were compared with spatial data such as distance …

Sexual Dimorphism Of Thermal Preference In Florida Scrub Lizards (Sceloporus Woodi) And Predicting Response To Climate Change In Two Rare Habitats, Sidney E. Anderson

College of Graduate Studies: Theses & Dissertations

The Florida Scrub Lizard (Sceloporus woodi) is endemic to Florida, where it inhabits fragments of xeric sandhill uplands including endangered long-leaf pine and sand pine scrub habitats. Lizards depend on a predictable range of temperatures to maximize their growth and productivity, and to do so, they shuttle among various thermal micro-environments. Thus, the spatial distribution of temperatures in the habitat is important. Habitats dominated by either high or low extremes of an organism’s preference are energetically costly and dangerous (less optimal), especially to gravid females. This study examines thermal preference of a near-threatened species that also inhabits increasingly rare habitats. …

The Effect Of Jatropha Multifida L. As A Natural Dietary Additive On Anthelmintic Activity And Performance In Lambs Infected By Trichostrongylus Spp., Juju Julaeha, Diky Ramdani, Endang Yuni Setyowati

Turkish Journal of Veterinary & Animal Sciences

Jatropha multifida leaf powder (JMLP) is rich in phenolic tannins and it has the potential to be a natural dietary additive for ruminants. A 4 × 5 factorial design was used to study the effects of 4 different doses of JMLP treatment (dry matter, DM basis) at 0% (JMLP-0), 0.5% (JMLP-0.5), 0.75% (JMLP-0.75), and 1% (JMLP-1) in the diet of ewe lambs infected by Trichostrongylus spp. at 2332 ± 248 eggs/g feces on fecal egg counts (FEC, eggs/g feces) of 5 different measurement times (0, 14, 28, 42, and 84 days) using 5 replicates. Average daily gain (ADG, g/head/day) and …

Effect Of Rice Distillers Dried Grains With Solubles And Rice Gluten Meal Along With Enzyme Supplementation On The Growth Performance, Nutrient Utilization, Production Efficiency, And Cost Economics Of Broiler Chicken Production, Om Prakash Dinani, Pramod Kumar Tyagi, Nasir Akbar Mir, Asit Baran Mandal, Divya Sharma, Rokade Jaydip Jaywant

Turkish Journal of Veterinary & Animal Sciences

An experiment was conducted to evaluate the effect of feeding rice distillers dried grains with solubles (rDDGS) and rice gluten meal (RGM) combination with enzyme supplementation on the production performance of broiler chickens. The experimental diets consisted of the following: T1 (no rDDGS/RGM/enzyme), T2 (no rDDGS/RGM, multienzyme), T3 (12.5% rDDGS, 15.0% RGM, no enzyme), T4 (12.5% rDDGS, 15.0% RGM, protease enzyme), T5 (10.0% rDDGS, 12.5% RGM, no enzyme), and T6 (10.0% rDDGS, 12.5% RGM, protease enzyme). Each treatment was assigned 6 replicate groups of birds randomly with 8 birds in each group (48 birds/ treatment). The results revealed the poor …

Investigation Of G1 (C.260g>A) Polymorphism In Exon 1 Of Gdf9 Gene In Turkish Sheepbreed Karayaka, Koray Kirikçi, Mehmet Aki̇f Çam, Levent Mercan

Turkish Journal of Veterinary & Animal Sciences

GDF9 is an essential gene for normal follicular development and prolificacy in sheep. The aim of this study was to determine the genetic polymorphism and G1 mutation of GDF9 gene exon 1 in 100 Karayaka ewes, belonging to four subpopulations in the Black Sea Region provinces of Turkey. Based on DNA sequence results, two alleles (A and G) and two genotypes (GG and GA) were determined with the restriction fragment length polymorphism analysis of PCR-amplified fragments (PCR-RFLP) method. The frequencies of mutant (A) and wild-type (G) alleles were detected as 0.10 and 0.90. The frequencies of GG and GA genotypes …

Determination Of Slaughter And Carcass Traits In Male Turkish Anatolian Buffaloes Atdifferent Slaughter Weights, Zafer Ulutaş, Azi̇z Şahi̇n, Yüksel Aksoy, Emre Uğurlutepe

Turkish Journal of Veterinary & Animal Sciences

Nowadays, deficits in red meat resources in Turkey are caused by the import of red meat from different countries. Anatolian buffaloes used in this research are a species that can contribute to red meat production in Turkey. This study was performed to determine the slaughter and carcass traits of Anatolian buffaloes at different slaughter weights. Twenty weaned male Anatolian buffalo calves of about 5 months old, at an average live weight of 100 kg, were used as the animal specimen in the present study. The calves were fed with 30:70 roughage (alfalfa hay = 18.07% crude protein, 2186.90 kcal kg$^{-1}$ …

Effect Of Different Doses Of Hydrated C$_{60}$ Fullerene Nanoparticles On Ram Semen Duringcool Storage, Şeyma Özer Kaya, İbrahi̇m Hali̇l Güngör, Serap Dayan Ci̇nkara, Tutku Can Acisu, Recep Hakki Koca, Serkan Ali̇ Akarsu, Coşkun Can, Aslihan Çakir, İrfan Yilmaz, Muhammed Sami̇ Halici, Seyfetti̇n Gür, Mustafa Sönmez, Gaffari̇ Türk

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effect of different doses of hydrated C$_{60}$ fullerene (C$_{60}$HyFn) nanoparticles on ram semen during cool storage at 5 °C. Semen samples were collected from 7 healthy Akkaraman rams by artificial vagina during nonbreeding season (April) 3 times for two weeks (3 replications), and samples having good initial sperm motility (> 70 %) and count (> 2 billion / mL) were pooled and diluted with tris-egg yolk based extender at 37 °C. Then, the diluted semen samples were divided into 15 aliquots control and 14 different C60HyFn groups (25 nM, 50 nM, …