Digital Commons Network^™

Crop Registration: The Pathway To Public Access Of Plant Genetic Materials To Build Crops For The Future, D. D. Ellis, K. A. Garland-Campbell, J. A. Grotenhuis, M. M. Jenderek, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Starting in 1926 and continuing for 80+ yr, 11,241 crop genetic materials have been registered as of 31 Dec. 2008. The crop registration process is an important pathway to publically describe and document new and useful genetic materials and to incorporate these into the public domain via the National Plant Germplasm System (NPGS). Crop Registration materials are now searchable via the Germplasm Resources Information Network (GRIN) and demand for registered materials remains strong with more than 9150 registered accessions distributed in the past 26 yr by the NPGS. Guidelines continue to evolve to accommodate global factors effecting germplasm exchange such …

Efficacy Of Singular And Stacked Brown Midrib 6 And 12 In The Modification Of Lignocellulose And Grain Chemistry, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

In sorghum, brown midrib (bmr) 6 and 12 impair the last two steps of monolignol synthesis. bmr genes were introduced into grain sorghum to improve the digestibility of lignocellulosic tissues for grazing or bioenergy uses following grain harvest. Near-isogenic grain sorghum hybrids (AWheatland X RTx430) were developed containing bmr6, bmr12, and the bmr6 bmr12 double mutant (stacked), and their impacts were assessed in a two-year field study. The bmr genes did not significantly impact grain or lignocellulosic tissue yield. Lignocellulosic tissue from bmr6, bmr12, and stacked hybrids had reduced lignin content and increased in …

Heterosis In Sweet Sorghum And Selection Of A New Sweet Sorghum Hybrid For Use In Syrup Production In Appalachia, T. W. Pfeiffer, M. J. Bitzer, J. J. Toy, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Although heterosis is well established in grain and forage sorghum [Sorghum bicolor (L.) Moench], reports of heterosis in sweet sorghum are limited to results from grain sorghum × sweet sorghum hybrids. Recent development of cytoplasmic male-sterile sweet sorghum lines allows creation of sweet sorghum hybrids for research and industry. Male sterility may also affect allocation of photosynthate to plant parts, creating the potential to increase sugar content in stems by eliminating seed as a sink. The objectives of this study were to compare performance of A₃ cytoplasmic male-sterile lines and A₃ cytoplasmic male-sterile hybrids to fertile B …

Influences Of Wind And Sandblasting On The Endangered Blowout Penstemon, James Stubbendieck, Cheryl D. Dunn, Heidi L. Hillhouse, L. M. Landholt

Department of Agronomy and Horticulture: Faculty Publications

Blowout penstemon Penstemon haydenii is a federally endangered species growing only in areas with active wind erosion in sand dunes of the central United States. This early seral species declines as the blowout habitat stabilizes, allowing later seral species to increase. Blowout penstemon populations and plant size declined in the 1990s when precipitation was higher than normal, resulting in reduced sand movement. We conducted a greenhouse experiment to determine whether blowing sand influenced vigor and persistence of blowout penstemon seedlings. Treatments were wind, sandblasting, wind with sandblasting, and a control. The wind treatment was a constant 14 km h^{–1 …}

Fine Mapping Of The Scn Resistance Locus Rhg1-B From Pi 88788, Myungsik Kim, David L. Hyten, Andrew F. Bent, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is the most economically damaging soybean [Glycine max (L.) Merr.] pest in the USA and genetic resistance is a key component for its control. Although SCN resistance is quantitative, the rhg1 locus on chromosome 18 (formerly known as Linkage Group G) confers a high level of resistance. The objective of this study was to fi ne-map the rhg1-b allele that is derived from plant introduction (PI) 88788. F₂ and F₃ plants and F_3:4 lines from crosses between SCN resistant and susceptible genotypes were tested with genetic markers to …

High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of …

Inoculation With Arbuscular Mycorrhizal Fungi Or Crop Rotation With Mycorrhizal Plants Improves The Growth Of Maize In Limed Acid Sulfate Soil, Hasao Higo, Katsunori Isobe, Dong-Jin Kang, Kazuhiro Ujie, Rhae A. Drijber, Ryuichi Ishii

Department of Agronomy and Horticulture: Faculty Publications

Arbuscular mycorrhizal fungi (AMF) improve the uptake of immobile mineral nutrients such as phosphate, thereby improving plant growth. In acid sulfate soil (ASS), AMF spore density is generally low which impacts root colonization and phosphate uptake. Thus, inoculation may help increase AMF colonization of crops grown in ASS. AMF spore density decreases after cultivation of a non-host crop or bare fallow. In addition, preceding crops affect the growth and yield of subsequent crops. The production of AMF inocula requires AMF-compatible plants. The objective of the present study is to elucidate the effect of preceding crops on the persistence of inoculated …

Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping …

Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map-based cloning and for marker-assisted selection in plant breeding. The objectives of this study were to determine the abundance of SSRs in the soybean genome and to develop and test soybean SSR markers to create a database of locus-specific markers with a high likelihood of polymorphism. A total of 210,990 SSRs with di-, tri-, and tetranucleotide repeats of five or more were identified in the soybean whole genome sequence (WGS) which included 61,458 SSRs consisting of repeat units of di- (≥10), tri- (≥8), and tetranucleotide (≥7). Among …

Phylloplanins: Novel Antifungal Proteins On Plant Leaf Surfaces, Ryan William Shepherd

University of Kentucky Doctoral Dissertations

Secreted surface proteins are an innate immune defense component employed by animals to inhibit invading microbes. Surface proteins have not been documented in plants, even though the aerial leaf surface, or phylloplane, is a major site of pathogen ingress. We have discovered novel proteins, termed phylloplanins, which accumulate on leaf surfaces of Nicotiana tabacum, and we have isolated the gene Phylloplanin that is unique in gene databases. Natural and E. coli-expressed phylloplanins inhibit spore germination and limit leaf infection by the oomycete pathogen Peronospora tabacina.

We investigated the site of phylloplanin biosynthesis using biochemical techniques. These techniques …

Whole Genome Wide Expression Profiles Of Vitis Amurensis Grape Responding To Downy Mildew By Using Solexa Sequencing Technology, Jiao Wu, Yali Zhang, Huiqin Zhang, Hong Huang, Kevin M. Folta, Jiang Lu

School of Information Faculty Publications

Background: Downy mildew (DM), caused by pathogen Plasmopara viticola (PV) is the single most damaging disease of grapes (Vitis L.) worldwide. However, the mechanisms of the disease development in grapes are poorly understood. A method for estimating gene expression levels using Solexa sequencing of Type I restriction-endonuclease-generated cDNA fragments was used for deep sequencing the transcriptomes resulting from PV infected leaves of Vitis amurensis Rupr. cv. Zuoshan-1. Our goal is to identify genes that are involved in resistance to grape DM disease.

Results: Approximately 8.5 million (M) 21-nt cDNA tags were sequenced in the cDNA library derived from PV pathogen-infected …

Lucerne Guidelines For Western Australia Principles For Integrating A Perennial Pasture Into Broadacre Dryland Farming Systems, Diana Fedorenko, Perry Dolling, Christopher Loo, Tom Bailey, Roy Latta

Bulletins 4000 -

Lucerne, often regarded as the queen of forages, has been used in agriculture for centuries. In Western Australia, however, its potential has never been fully realised despite its well-documented ability to dewater soils and reduce groundwater recharge. Over the years adoption has been limited by a range of factors including pests and diseases, soil acidity, grazing management and economics. During the last decade research has addressed these issues, paving the way for much greater impact at the farm and catchment level.

Heirloom And Hybrid Tomato Yield And Quality In Organic And Conventional Production Systems, Diana J. Edlin

Masters Theses & Specialist Projects

Due to the recent changes in the economy of Kentucky tobacco production, some producers are seeking an alternative crop that will provide similar economic gains to tobacco without needing more acreage. Tomatoes are an existing crop in Kentucky that have been declining in acreage over the last five years. There is evidence to suggest that, through niche and local marketing, tomatoes may be able to fill the void left by tobacco. However, there is concern among producers that they will lose yield and/or quality if they switch to one of these niche production systems or cultivars.

A two year study …

Detection And Measurement Of Water Stress In Vegetation Using Visible Spectrum Reflectance, Arthur Zygielbaum

Department of Geography: Dissertations, Theses, and Student Research

At any scale, from a single microbe to the planet that nurtures us, water defines our place in the universe. It provides the hydraulic forces needed to give plants structure, and the medium enabling photosynthesis, the basis for most life on Earth, to occur. Knowledge of plant water status is vital to understanding the state or condition of vegetation, information which is essential to disciplines as diverse as agriculture, geography, and climatology. Non-destructive and remote sensing of plant water status allows the gathering of such information across wide geographic extents and over long periods of time. Monitoring vegetation remotely requires …

Herbicide, Salinity, And Flooding Tolerance Of Foxtail Barley (Hordeum Jubatum L.) And Desirable Pasture Grasses, Karl R. Israelsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Research trials performed in the greenhouse compared the tolerance and response of Hordeum jubatum and desirable pasture grass species to herbicides, salinity, and flooding. Desirable grass species used in this study included: 'Fawn' tall fescue (Festuca arundinaceae), 'Garrison' creeping foxtail (Alopecurus arundinaceus), 'Palaton' reed canarygrass (Phalaris arundinacea), 'Climax' timothy (Phleum pratense), 'Alkar' tall wheatgrass (Thinopyrum ponticum), 'Potomac' orchardgrass (Dactylis glomerata), and 'Mustang' altai wildrye (Leymus angustus). Tolerance to herbicides, salinity, and flooding varied significantly among grass species. Herbicide tolerance was tested using four herbicides at five …

Effect Of Different Schedules Of Baby Corn (Zea Mays L.) Harvests On Baby Corn Yield, Grain Yield, And Economic Profit Value, Zheng Wang

Masters Theses & Specialist Projects

Maize (Zea mays L.) ranks third as a food crop after wheat and rice and is characterized not only as a cereal crop but also as a vegetable. Maize used as a vegetable is known as “baby corn”. Baby corn consists of unfertilized young ears harvested 2 or 3 days after silk emergence. The present study was implemented in 2009 at Western Kentucky University Agriculture Research and Education Center (36.93 N, 86.47 E) in Bowling Green, Kentucky. The purpose of the study was to compare the effect of different schemes of harvest on baby corn (BC) yield, grain maize (GM) …

No-Till Tomato Production, Bradley L. Booker

Master's Theses

Tillage increases erosion rates and diminishes the quality of soils but has traditionally been a way to manage weeds and prepare a seedbed. No-till vegetable production can ameliorate the ill-effects of tillage in an economically effective way but has not been studied much in California. The objective of this thesis was to determine the viability of no-till vegetable cropping on the Central Coast of California. Tomatoes were grown in no-till and conventionally tilled treatments. Total yield, fruit weight, weed emergence, soil bulk density, soil aggregate stability, and soil organic carbon content were measured and compared. Yields and soil tests in …

Summaries Of Arkansas Cotton Research 2008, Derrick M. Oosterhuis

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Impact Of Weed Management Practices On Grapevine Growth, Yield Components, Plant And Arthropod Abundance, And Carabid Seed Predation In Paso Robles Vineyard, Paolo Sanguankeo

Master's Theses

In the Central Coast of California, USA, wine grape growers are making efforts to identify weed control practices that promote biodiversity in their vineyards while maintaining yields. A field study was conducted in Paso Robles, CA in 2006 and 2007 evaluating the effect on Zinfandel grape-vine growth and production, groundcover plant, and ground dwelling arthropod communities of five weed control practices: 1) flumioxazin, 2) simazine, 3) cultivation, 4) cover crop, and 5) untreated control.

The herbicide treatments had the lowest weed biomass followed by the cultivation, being approximately 10 and 2 times lower than the weed biomass of either the …

Effect Of Water On Micronutrient Content And Yield In Rice (Oryza Sativa L.), Sheila Lorraine Baker

Master's Theses

Rice (Oryza sativa L.) is an important crop in many countries. According to the IRRI, more than 2 billion people globally depend on rice as a staple food (Dawe, et al. 2003). Studies on micronutrient content in rice and the effect of water in availability of the nutrients may aid in decreasing global nutrient deficiencies. Rice is grown under different water regimes such as AWD and intermittent flooding, sprinkler and furrow irrigation. A greenhouse pot experiment of rice utilizing a split plot design under different water regimes was conducted to assess the affect of water on (1) DTPA extractable soil …

Effect Of Environmental Density And Buoyancy On Growth And Gravitropic Response In Maize Roots, Jessy Robbins

All-Inclusive List of Electronic Theses and Dissertations

The mechanism by which plants sense gravity is not fully understood. The statolith theory states that plants sense gravity based on the sedimentation of starch granules called statoliths. However, not all plants contain statoliths. The hydrostatic model was proposed to explain gravitropic sensing in systems which lack statoliths. This model states that the gravity sensor in a plant cell is the entire protoplast; gravitropic sensing depends on the buoyancy of the protoplast relative to the density of its external environment. The ability of maize roots to sense gravity when grown in media of differing densities is examined in these studies. …

Estimating Water Application Efficiency For Drip Irrigation Emitter Patterns On Banana, Alisson Jadavi Pereira Da Silva, Eugênio Ferreira Coelho, Jarbas Honorio De Miranda, Stephen Ray Workman

Biosystems and Agricultural Engineering Faculty Publications

The objective of this work was to evaluate root and water distribution in irrigated banana (Musa sp.), in order to determine the water application efficiency for different drip irrigation emitter patterns. Three drip emitter patterns were studied: two 4-L h^-1 emitters per plant (T1), four 4-L h^-1 emitters per plant (T2), and five 4-L h^-1 emitters per plant (T3). The emitters were placed in a lateral line. In the treatment T3, the emitters formed a continuous strip. The cultivated area used was planted with banana cultivar BRS Tropical, with a 3-m spacing between rows and a …

An Integrated Approach To Model The Establishment, Water Use And Growth Of New Perennial Pasture Species, Lalith D B Suriyagoda, M H. Ryan, Daniel Real, Hans Lambers, Michael Renton

Conference papers and presentations

Perennial pastures increase the sustainability of Australian cropping systems by increasing out of season water use, improving ground cover and helping to avoid dryland salinity. Medicago sativa (lucerne) is the most common perennial legume pasture and is adapted to a wide range of environments, but it does not persist well in areas with acid soils or low summer rainfall (Cocks 2001). Under these scenarios, native herbaceous perennial legumes, such as Cullen australasicum (Cocks 2001), and the exotic perennial pasture legume Bituminaria bituminosa var. albomarginata (common name albo-tedera) are potential alternatives to the currently limited perennial forage options. However, the growth …

Best Management Practices For Corn Production In South Dakota: Websites With Related Information, David E. Clay, Kurtis D. Reitsma

SDSU Extension Circulars

No abstract provided.

Best Management Practices For Corn Production In South Dakota: Recordkeeping, James A. Wilson, Kurtis D. Reitsma, David E. Clay

SDSU Extension Circulars

Recordkeeping is an important component of all crop production systems. The time that is spent maintaining careful records can help to improve the production, profit, and overall efficiency of the production enterprise. Records provide information needed to identify successes and failures.

Best Management Practices For Corn Production In South Dakota: Corn Grain Harvest, Daniel S. Humberg, Richard E. Nicolai, Kurtis D. Reitsma

SDSU Extension Circulars

Corn (grain) harvest can begin when grain moisture drops below 30%. However, most producers will allow corn to dry in the field until grain moisture is between 18 to 25%. Harvesting corn when grain moisture levels are high can result in excessive drying costs, kernel damage, and harvest loss from improper threshing. Allowing corn to stay in the field too long can result in excess harvest loss from stalk lodging, ear drop, or kernel shattering. An optimal harvest depends not only on the condition of the crop but also on the proper maintenance and adjustment of harvest and grain handling …

Best Management Practices For Corn Production In South Dakota: Corn Insect Pests, Michael A. Catangui, Billy W. Fuller, B. Wade French

SDSU Extension Circulars

Historically, the major corn insect pests have been corn rootworms (northern and western), European corn borer, and black cutworm. Bt-corn hybrids are effective against most of these pests. However, Bt-corn hybrids are not effective against corn leaf aphid, corn root aphid, sap beetles, corn rootworm adults, grasshoppers, white grubs, wireworms, seed corn beetle, and seed corn maggots. These insect pests can reduce corn yields. This chapter discusses the management and biology of important corn insect pests commonly observed in South Dakota.

Best Management Practices For Corn Production In South Dakota: Soil Fertility, David E. Clay, Kurtis D. Reitsma

SDSU Extension Circulars

Corn requires sufficient amounts of at least 14 nutrients for optimal production (fig. 7.1). Soil fertility strategies should consider soil residual plant nutrients, cost of fertilizer relative to the value of corn, and management techniques that increase efficiency.

Best Management Practices For Corn Production In South Dakota : Corn Calendar And Troubleshooting Guide, Troy Bauder, Dwayne L. Beck, Sue L. Blodgett, C. Gregg Carlson, Michael A. Catangui, David E. Clay, Sharon A. Clay, Darrell L. Deneke, Martin A. Draper, B. Wade French, Billy W. Fuller, Robert G. Hall, Curt A. Hoffbeck, Daniel S. Humburg, Marie A. Langham, Douglas D. Malo, Mike J. Moechnig, Richard E. Nicolai, Kurtis D. Reitsma, Bradley E. Ruden, Thomas E. Schumacher, Dennis P. Todey, Todd P. Trooien, Hal D. Werner, James A. Wilson, Howard J. Woodard, Leon J. Wrage

SDSU Extension Circulars

No abstract provided.

Best Management Practices For Corn Production In South Dakota : Corn Drying And Storage, Troy Bauder, Dwayne L. Beck, Sue L. Blodgett, C. Gregg Gregg

SDSU Extension Circulars

The goal for a corn drying and storage system is to maintain grain quality without impeding harvesting or shipping. This chapter describes the factors that influence grain quality, proper handling techniques, drying procedures, storage management, and safety precautions.