Digital Commons Network^™

Mushroom Inoculation On Switchgrass Feedstock During Storage: Effects Of Subsequent Pre-Processing For Intended Biofuels Production, Amandeep Singh Turay

Graduate Theses and Dissertations

The objective of this project was to examine the effect of fungal treatment and liquid hot water pretreatment of switchgrass combine in view of increasing glucose release. The fungal treatment consisted of incubating Pleurotus ostreatus in square switchgrass bales, at 50% moisture content for 25 days, 54 days, and 82 days. The digestibility of the switchgrass biomass was subsequently evaluated using Accelerase 1500 enzyme. Lignin is an important barrier to enzymatic hydrolysis, and it was stipulated that incubation with P. ostreatus would disrupt plant cell walls, resulting in enhanced saccharification. Three different concentrations of P. ostreatus were evaluated: 0%, 2%, …

Evaluation Of Screening Methods To Detect Heat Stress In Diverse Cotton Genotypes, Mathilda M. Van Der Westhuizen

Graduate Theses and Dissertations

Cotton (Gossypium hirsutum L.) is sensitive to heat stress (HS) during reproductive development. The objective of this study was to evaluate different screening methods for identification of heat tolerance in cotton genotypes. Three growth chamber studies and four field trials were conducted from 2014 to 2017 using genotypes Arkot 9704, VH260, DP 210 B2RF and DP393. Measurements were made of membrane leakage (ML), chlorophyll fluorescence (ChlF), glutathione reductase (GR), and sucrose concentration. In the growth chambers, measurements were made at 30 and 40°C and at 2, 4 and 6 hours of HS, as well as 3 and 7 days after …

B. R. Wells Arkansas Rice Research Studies 2016, R. J. Norman, K.A. K. Moldenhauer

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Molecular And Culture-Based Assessment Of Root/Crown Rot Fungal/Oomycete Complex Of Bean In Mozambique, Suzana Vanessa Ismael Fernandes

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Dry edible beans (Phaseolus vulgaris) play an important role in food security, alleviating hunger and malnutrition and adding value to the economy in Mozambique. Recently, root/crown rot (RCR) has caused yield losses in bean fields in Mozambique. A diverse taxa of fungi and oomycetes have been associated with RCR. However, in Mozambique little information on root rot is available. The purpose of this study is to use Illumina sequencing platform, polymerase chain reaction (PCR) and culture-based methods to identify the primary pathogens associated with RCR in Mozambique. A total of 88 plants showing symptoms of RCR disease were …

Induction And Suppression Of Antiviral Rna Silencing By Tomato Spotted Wilt Virus, Sergio M. Gabriel Peralta

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Tomato spotted wilt virus (TSWV) is an emerging pathogen with wide host range and one of the most important viruses of plants. Information regarding processing of negative single stranded RNA viruses such as TSWV in the RNA silencing pathway remains limited. In nature TSWV is only transmitted by thrips as vectors and since infection occurs in both thrips and plants, an experimental system to transmit using thrips and the detection of TSWV were established. In order to understand the processing of TSWV in the RNA silencing pathway, Arabidopsis thaliana as a model plant was used in the genetic analysis against …

Early Growth Stages Salinity Stress Tolerance In Cm72 X Gairdner Doubled Haploid Barley Population, Tefera T. Angessa, Xiao-Qi Zhang, Gaofeng Zhou, Sue Broughton, Wenying Zhang, Chengdao Li

Grain and Other Field Crops Research Articles

A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H–6H each …

Genomic Approaches For Improvement Of Understudied Grasses, Keenan Amundsen, Gautam Sarath, Teresa Donze-Reiner

Biology Faculty Publications

No abstract provided.

Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff

Department of Agronomy and Horticulture: Faculty Publications

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.

Geometry-Based Mass Grading Of Mango Fruits Using Image Processing, M. A. Momin, Md Towfiqur Rahman, M. S. Sultana, C. Igathinathane, A. T. M. Ziauddin, T. E. Grift

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Mango (Mangifera indica) is an important, and popular fruit in Bangladesh. However, the post-harvest processing of it is still mostly performed manually, a situation far from satisfactory, in terms of accuracy and throughput. To automate the grading of mangos (geometry and shape), we developed an image acquisition and processing system to extract projected area, perimeter, and roundness features. In this system, images were acquired using a XGA format color camera of 8-bit gray levels using fluorescent lighting. An image processing algorithm based on region based global thresholding color binarization, combined with median filter and morphological analysis was developed …

Transcriptomic Resources For The Medicinal Legume Mucuna Pruriens: De Novo Transcriptome Assembly, Annotation, Identification And Validation Of Est-Ssr Markers, N. Sathyanarayana, Ranjith Kumar Pittala, Pankaj Kumar Tripathi, Ratan Chopra, Heikham Russiachand Singh, Vikas Belamkar, Pardeep Kumar Bhardwaj, Jeff J. Doyle, Ashley N. Egan

Department of Agronomy and Horticulture: Faculty Publications

Background:

The medicinal legume Mucuna pruriens (L.) DC. has attracted attention worldwide as a source of the anti-Parkinson’s drug L-Dopa. It is also a popular green manure cover crop that offers many agronomic benefits including high protein content, nitrogen fixation and soil nutrients. The plant currently lacks genomic resources and there is limited knowledge on gene expression, metabolic pathways, and genetics of secondary metabolite production. Here, we present transcriptomic resources for M. pruriens, including a de novo transcriptome assembly and annotation, as well as differential transcript expression analyses between root, leaf, and pod tissues. We also develop microsatellite markers …

A Comprehensive Analysis Of Alternative Splicing In Paleopolyploid Maize, Wenbin Mei, Sanzhen Liu, James C. Schnable, Cheng-Ting Yeh, Nathan M. Springer, Patrick S. Schnable, William Brad Barbazuk

Department of Agronomy and Horticulture: Faculty Publications

Identifying and characterizing alternative splicing (AS) enables our understanding of the biological role of transcript isoform diversity. This study describes the use of publicly available RNA-Seq data to identify and characterize the global diversity of AS isoforms in maize using the inbred lines B73 and Mo17, and a related species, sorghum. Identification and characterization of AS within maize tissues revealed that genes expressed in seed exhibit the largest differential AS relative to other tissues examined. Additionally, differences in AS between the two genotypes B73 and Mo17 are greatest within genes expressed in seed. We demonstrate that changes in the level …

Effects Of Drought Conditions On Microbial Communities In Native Rangelands, Abbie L. Lasater, Tiffany Carter, Charles Rice

Chemical Engineering Undergraduate Honors Theses

Climate change is a result of greenhouse gases released into the atmosphere. These changes are expected to cause extreme weather conditions, including severe storms. Large amounts of rain will fall in shorter periods of time, leading to heavy runoff, and increasing the severity of drought conditions within the soil (Zeglin et al. 2013).

Native grasslands occupy almost a quarter of the earth’s land surface and are valuable ecological resources. They contain soils with high concentrations of organic matter and play a key role in mitigating greenhouse gas emissions through carbon sequestration. There are a variety of grassland management techniques including …

Temporal Evaluation Of Corn Respiration Rates Using Pressure Sensors, Gagandeep Singh Ubhi

Graduate Theses and Dissertations

High respiration rate of a grain indicates faster degradation of its dry matter. Proper grain management requires chronological and precise measurements of carbon dioxide evolved from grain respiration during the postharvest storage duration. Therefore the main goal of this research was to develop a new technique that evaluates temporal corn respiration rate using pressure sensors. It was based on measuring pressure drop associated with the grain respiration in a closed container and using it to calculate the grain respiration rates.

Dry corn (Zea Mays L.) was procured from a local farmer and stored at 4ºC. Corn rewetting technique was applied …

Evaluation Of Benzobicyclon For Use In Midsouthern Rice (Oryza Sativa) Systems, Mason Luke Young

Graduate Theses and Dissertations

A new herbicide site of action (SOA) is needed by rice (Oryza sativa L.) producers in the Midsouth for the control of problematic and herbicide-resistant weeds. Currently, six problematic weeds are relevant to Midsouthern rice producers because of resistance to at least one SOA, if not more. Gowan Company is in the process of commercializing benzobicyclon for use in Midsouthern rice systems. Benzobicyclon, a Group 27 post-flood herbicide, controls a broad spectrum of aquatics, broadleaves, grasses, and sedges, including those currently resistant to Group 2 herbicides. This will be the first 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicide commercially available in US rice …

The Effect Of Spray Parameters On The Application Of Enlist Duo, Matthew R. Nelson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The emergence of new weed control challenges, along with shifts in weed management strategies and cultural practices, has resulted in an increased reliance on chemical weed control in United States (US) cropping systems. As a result, numerous weed species have evolved resistance to herbicides such as glyphosate, thus prompting the development of new weed control systems designed to aid growers in managing resistant weeds. While these new weed control options may give growers additional management options, the high sensitivity of broadleaf crops, fruits, and vegetables to products containing 2,4-D or dicamba increases the potential for herbicide drift resulting from application …

Evaluation Of Safening Effects To Herbicides Conferred Via Insecticide Seed Treatments In Soybean (Glycine Max) And Grain Sorghum (Sorghum Bicolor), Nicholas Robert Steppig

Graduate Theses and Dissertations

Interactions between herbicides and insecticides in crop production have been documented for a number of years. Research has shown that applications of some organophosphate insecticides at planting can reduce cotton injury following applications of the soil-applied herbicide clomazone. Additionally, recent research has shown that, when applied as seed treatments prior to planting, some neonicotinoid insecticides can safen rice to drift from both glyphosate and imazethapyr. Since insecticide seed treatments are commonly used in many crop production systems throughout the Midsouth, exploring their ability to reduce injury from herbicides in other crops besides rice is of great interest. Presently no research …

Value Of Cover Crops In Suppressing Weeds And Protecting Cotton Yields And Likelihood Of Residual Herbicide Carryover To Cover Crops, Matheus Gabriel Palhano

Graduate Theses and Dissertations

Weed-resistance management has become a topic of concern for modern agriculture. Cost related to herbicide usage has increased greatly due to evolution and proliferation of resistant weeds. Therefore, experiments were conducted to investigate the potential for using cover crops to suppress problematic weeds in cotton as well as chemical options for cover crop dessication, and sensitivity of cover crops to residual herbicides were evaluated. No differences were observed for cereal rye biomass production and consequently weed suppression between broadcast and drilled planting methods. Total amount of cover crop biomass was vital to effectively suppress weeds. Hence, of the cover crops …

Characterization Of Candidate Compound For Control Of Barnyardgrass And Other Troublesome Weeds In Rice, Michael Ryan Miller

Graduate Theses and Dissertations

Barnyardgrass remains one of the most difficult weeds to manage in rice. As herbicide resistance in this species, as well as others, continues to increase new control options are needed. Dow AgroSciences recently discovered and is developing a new herbicide for rice which is the primary focus of this research. Experiments were conducted to characterize this new herbicide for its use and effectiveness in midsouthern U.S. rice systems. The research conducted herein covers various aspects of herbicide characterization including: barnyardgrass dose response, accession testing, spectrum of control and tank-mix capability, application optimization, soil carryover and plant back determination, drift onto …

Evaluation And Association Analysis Of Cowpea Salt Tolerance, Waltram Second Ravelombola

Graduate Theses and Dissertations

Cowpea [Vigna unguiculata (L.) Walp.] (2n=2x=22) is a leguminous crop providing inexpensive protein for human consumption that can be grown worldwide. Salinity is one of the major threats to cowpea production, particularly in semi-arid regions of Africa. Salinity significantly affects seed germination and decreases pod yield in cowpea. However, little has been done to provide farmers salt-tolerant cowpea cultivars. Knowledge on the genetics of cowpea salt tolerance is very limited. This study aimed to:(1) evaluate cowpea salt tolerance at germination stage, (2) evaluate cowpea salt tolerance at seedling stage, (3) conduct an association study for cowpea salt tolerance at germination …

Determining The Effects Of Sulfur Fertility Levels On Edamame Soybean [ Glycine Max ( L. ) Merrill ] Protein Components, Theoneste Nzaranyimana

Theses and Dissertations

Edamame [Glycine max (L.) Merr.] a vegetable type soybean is harvested and consumed before reaching its maturity stage (R6). Its health properties drew attention to U.S. consumers because of its high protein content. Proteins are the major structural component of all cells in the living organisms. They are made of essential and non-essential amino acids which play a big role in growth and development and are source of energy. Proteins act as enzymes, antibodies, and hormones and blood protein. Sulfur (S) is a component of the amino acids cysteine (Cys) and methionine (Met) which are the building blocks for proteins. …

Respons Pertumbuhan Tanaman Aren (Arenga Pinnata Merr.) Dengan Pemberian Pupuk Organik Dan Pupuk Anorganik Berbeda Dosis, Mira Ariyanti, Mochamad Arief Soleh, Yudithia Maxiselly

Jurnal Kultivasi

Tanaman aren (Arenga pinnata Merr.) adalah salah satu tanaman perkebunan yang bernilai ekonomis tinggi dan potensial untuk terus dikembangkan. Salah satu upaya untuk meningkatkan produktivitas aren di Indonesia adalah dengan pemupukan. Pupuk anorganik yang lazim diaplikasikan pada tanaman aren memiliki kecenderungan untuk merusak kualitas tanah. Oleh karena itu, perlu dilakukan usaha untuk untuk mengembalikan kesehatan tanah dengan cara mengimbangi pemberian pupuk anorganik dengan pupuk organik. Penelitian ini dilakukan di kebun percobaan Ciparanje, Fakultas Pertanian Universitas Padjadjaran, Kecamatan Jatinangor Kabupaten Sumedang, Jawa Barat pada Oktober 2016 - Desember 2016. Penelitian menggunakan rancangan acak kelompok terdiri dari enam kombinasi perlakuan dan diulang …

Pengaruh Jarak Tanam Berbeda Dan Berbagai Dosis Pupuk Kandang Ayam Terhadap Pertumbuhan Dan Hasil Tanaman Hanjeli Pulut (Coix Lacryma-Jobi L.) Di Dataran Tinggi Punclut, Aep Wawan Irwan, Tati Nurmala, Tia Dian Nira

Jurnal Kultivasi

Penelitian ini bertujuan untuk melihat bagaimana pengaruh jarak tanam yang berbeda dan berbagai dosis pupuk kandang ayam serta memperoleh salah satu kombinasi jarak tanam dengan dosis pupuk kandang ayam yang memberikan pengaruh terbaik pada pertumbuhan dan hasil hanjeli pulut. Percobaan dilaksanakan dari bulan Desember 2015 sampai bulan Mei 2016 di Punclut, Lembang, Kabupaten Bandung dengan ketinggian tempat mencapai 1095 m di atas permukaan laut, dengan tanah Latosol dan tipe iklim C2 menurut klasifikasi Oldeman. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok (RAK) yang terdiri dari 9 perlakuan dan diulang sebanyak 3 kali, dengan perlakuan kombinasi jarak tanam …

Respons Tanaman Jagung (Zea Mays L.) Akibat Pemberian Pupuk Fosfat Dan Waktu Aplikasi Pupuk Hayati Mikroba Pelarut Fosfat Pada Ultisols Jatinangor, Agus Wahyudin, Betty Natalie Fitriatin, Fiky Yulianto Wicaksono, Ruminta, A. Rahadiyan

Jurnal Kultivasi

Jagung merupakan salah satu komoditas tanaman pangan yang mempunyai peranan strategis dalam pembangunan pertanian dan perekonomian Indonesia karena memiliki potensi dalam kebutuhan pangan, pakan, bahan baku industri, dan kerajinan tangan. Penelitian ini bertujuan untuk mengetahui pengaruh jenis pupuk fosfat dan waktu pengaplikasian pupuk hayati mikroba pelarut fosfat (MPF) terhadap pertumbuhan dan hasil tanaman jagung pada Ultisols Jatinangor. Percobaan dilaksanakan di Kebun Percobaan Ciparanje Fakultas Pertanian Universitas Padjadjaran, Jatinangor, Kabupaten Sumedang, Jawa Barat dengan ketinggian tempat +_ 750 meter di atas permukaan laut dan ordo tanah Ultisol, curah hujan rata-rata termasuk tipe C3 menurut Oldeman, dan temperatur udara berkisar antara 22,00 …

Modeling Gross Primary Production Of Midwest Maize And Soybean Croplands With Satellite And Gridded Weather Data, Gunnar Malek-Madani

Department of Geography: Dissertations, Theses, and Student Research

The gross primary production (GPP) metric is useful in determining trends in the terrestrial carbon cycle. Models that determine GPP utilizing the light use efficiency (LUE) approach in conjunction with biophysical parameters that account for local weather conditions and crop specific factors are beneficial in that they combine the accuracy of the biophysical model with the versatility of the LUE model. One such model developed using in situ data was adapted to operate with remote sensing derived leaf area index (LAI) data and gridded weather datasets. The model, known as the Light Use Efficiency GPP Model (EGM), uses a four …

Emergence, Competition, And Management Of Glyphosate-Resistant Common Ragweed (Ambrosia Artemisiifolia L.) In Nebraska Soybean, Ethann R. Barnes

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Common ragweed (Ambrosia artemisiifolia L.) is a competitive annual broadleaf weed in soybean (Glycine max) production fields throughout North America. The recent confirmation of glyphosate-resistant common ragweed in Nebraska justified the need to assess the emergence pattern and competitive ability of common ragweed in soybean and to evaluate alternative herbicide programs for effective management. The objectives of this research were to: 1) evaluate the effect of tillage and develop a predictive model for the emergence pattern of common ragweed in Nebraska; 2) model the competitive interaction between soybean and common ragweed as influenced by density and irrigation …

Detecting, Cloning, And Screening For Suppressors Of Rna Silencing In Maize Chlorotic Mottle Virus And Sugarcane Mosaic Virus, Nicole E. Bacheller

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Maize lethal necrosis disease (MLND) is one of the most important viral diseases of maize. MLND occurs when Maize chlorotic mottle virus (MCMV) co-infects the same plant with one of several potyviruses, including Sugarcane mosaic virus, Wheat streak mosaic virus or Maize dwarf mosaic virus. Originally prevalent in the Midwest and Peru in the 1970s, the disease was called corn lethal necrosis (CLN) and was controlled through breeding and sanitation. Recently, the disease has re-emerged in East Africa and is rapidly spreading and threatening the food sources of subsistence-farming populations. This re-emergence has raised several questions about the unknown …

High Throughput In Vivo Analysis Of Plant Leaf Chemical Properties Using Hyperspectral Imaging, Piyush Pandey, Yufeng Ge, Vincent Stoerger, James C. Schnable

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The possibility of predicting plant leaf chemical properties using hyperspectral images was studied. Sixty maize and 60 soybean plants were used, and two experiments were conducted: one with water limitation and the second with nutrient limitation, with the purpose of creating wide ranges of these chemical properties in plant leaf tissues. A hyperspectral imaging system with a spectral range from 550 to 1700 nm was used to acquire plant images in a high throughput fashion (plants placed on an automated conveyor belt). Leaf chemical properties were measured in the laboratory. Partial least squares regression was implemented on spectral data to …

Biomass Yield Of Switchgrass Cultivars Under High- Versus Low-Input Conditions, Michael D. Casler, Sergio Sosa, Lindsey Hoffman, Hilary Mayton, Calvin Ernst, Paul R. Adler, Arvid R. Boe, Stacy A. Bonos

Agronomy, Horticulture and Plant Science Faculty Publications

Switchgrass (Panicum virgatum L.) is undergoing development as a biomass crop to support conversion of cellulosic biomass to energy. To avoid the competition of biomass with food or feed crops, most commercialization proposals suggest that switchgrass should be grown exclusively on marginal lands that are not fit for food or feed production. The objective of this study was to investigate the potential for cultivar x environment interactions that would affect the methods and approaches for breeding and evaluating switchgrass cultivars, including both upland and lowland types, for high-input versus low-input types of environments. Biomass yield was measured on 14 cultivars …

Assessing Anther Extrusion And Its Effect On Us Hard Winter Wheat (Triticum Aestivum L.) Hybrid Seed Production, Nicholas Garst

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The promise of higher grain yields as a result of the development and production of hybrid wheat (Triticum aestivum L.) has not been fully realized primarily due to the high cost of seed production. Anther extrusion is a key trait that improves pollen availability, and thus, is expected to enhance hybrid wheat seed production yields. Hard winter wheat germplasm adapted to the US Great Plains was visually assessed for anther extrusion in the field and greenhouse environments. Significant genotypic differences were detected and high broad-sense heritability was calculated (ranging from 0.62 to 0.85) for anther extrusion in the field. …

The Lx Report Of The Bean Improvement Cooperative No. 60, March 2017.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

CONTENTS

LX Annual Report of the Bean Improvement Cooperative xi

BIC Coordinating Committee Membership - 1957 to 2017 . xii

BIC Meritorious Service and Distinguished Achievement Award Recipients - 1957 to 2015 xiii

BIC Awards Nomination Request for 2017 xvi

BIC/NAPIA Meeting in 2017 . xix

In Memory of Guillermo E. Galvez-Enriquez xx

RESEARCH PAPERS FOR 2017

OPTIMIZING SPORULATION OF Pseudocercospora griseola IN VITRO • Paula F. de Pádua1, Rafael Pereira1, Luanna B. W. Gomes1 and Elaine A. de Souza1* 1

FINE MAPPING THE BROAD SPECTRUM ANTHRACNOSE RESISTANCE GENE IN AMENDOIM CAVALO 3 • Gilio, T.A.S.1, Oscar P. Hurtado-Gonzales2, Giseli …