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Improvement Of The In Vitro Regeneration And Agrobacterium-Mediated Genetic Transformation Of Medicago Sativa L., Farzad Nofouzi, Muhammet Çağri Oğuz, Saber Delpasand Khabbazi, Ali̇ Ergül

Turkish Journal of Agriculture and Forestry

Alfalfa is a fodder crop that accounts for one of the best sources of protein and is widely cultivated around the world. In vitro regeneration of alfalfa has been studied earlier; however, most of the studies were almost intervened with callus formation. In this study, 3 explant sources (cotyledonary node, hypocotyl, and root crown) of two Turkish cultivars (Nimet and Savaş) were excised from young seedlings. Explants were subjected to different concentrations of BAP, BAP-IBA, and TDZ to evaluate the direct in vitro regeneration potential of selected plant parts. Moreover, we transformed the alfalfa plant with pBin19 harboring 35s.GUS-INT_35s.nptII construct …

Different Harvest Times Affect Market Quality Of Lycium Barbarum L. Berries, Ayşen Melda Çolak, Volkan Okatan, Mehmet Polat, Sultan Fi̇li̇z Güçlü

Turkish Journal of Agriculture and Forestry

Goji berry is a lesser-known fruit species and its berries have been used in traditional medicine for centuries as remedies to treat eye, liver, and kidney ailments, as well as for the prevention of cancer symptoms due to its high phytochemical content. This study aimed to investigate changes in the pomological, biochemical, and phytochemical properties of the berries of 4 goji berry genotypes harvested during 4 months (June, July, August, and September) of the same year. The results indicated that the highest berry dimensions (height and width) and soluble solid content were obtained from Genotype 2, harvested in June. The …

Effects Of Different Zinc Levels On Grain Yield And Some Phenological Characteristics Of Red Lentil (Lens Culinaris Medic.) Under Arid Conditions, Ayşe Gülgün Öktem

Turkish Journal of Agriculture and Forestry

This research aimed to determine the effects of different zinc (Zn) levels on the grain yield and some phenological characteristics of the Fırat-87 red lentil variety (Lens culinaris Medic.). The study was conducted during the winter months of 2013-2014 and 2014- 2015 in Zn-deficit areas of Şanlıurfa, Turkey. The experimental design was a randomized complete block design with 4 replicates. The Fırat-87 lentil variety and Zn sulfate (ZnSO4.7H2O) were used as the plant material and Zn source, respectively. The Zn levels used were the control (0 kg ha-1 Zn), 5 kg ha-1 Zn, 10 kg ha-1 Zn, 15 kg ha-1 …

Effects Of Cooking And Extra Virgin Olive Oil Addition On Bioaccessibility Of Carotenes Intomato Sauce, Merve Tomaş, Osman Sağdiç, Gi̇zem Çatalkaya, Derya Kahveci̇ Karincaoğlu, Esra Çapanoğlu Güven

Turkish Journal of Agriculture and Forestry

In this study, the effects of cooking and the addition of extra virgin olive oil on the in vitro bioaccessibility of carotenoids in tomato sauce were evaluated. The results indicated that total antioxidant activity, α-tocopherol, lycopene, β-carotene, lutein, and chlorophyll a contents were positively affected by the combination of cooking and addition of extra virgin olive oil. Moreover, after in vitro digestion, for intestinal phase, lycopene and Β-carotene were observed in all samples, whereas α-tocopherol and chlorophyll a were not detected in any of the samples. Lycopene (≈8.5-fold) and β -carotene (≈5-fold) were significantly improved (P < 0.05) by the effect of cooking and addition of extra virgin olive oil to the samples compared to control tomato sauce (without oil and cooking).

The Effects Of Mistletoe (Viscum Album L.) On The Physiological Properties Of Some Drupetrees In Turkey, Tamer Üstüner

Turkish Journal of Agriculture and Forestry

In the present study, the effects of mistletoe (Viscum album L.) on the physiological properties of drupe trees were investigated. In order to achieve this goal, sulfhydryl, chlorophyll a and b, total chlorophyll, ascorbic acid, total amino acid, proline, carotenoid, glucose, and fructose levels, as well as leaf proportional water contents were determined in both mistletoe and its hosts under laboratory conditions. According to the results, mistletoe caused a significant decrease in water content and reduced total ascorbic acid content in apricot, almond, and plum. Additionally, mistletoe did not affect the total amino acid, glucose, and fructose concentrations of the …

Effects Of Depth-Dependent Irrigation Regimes And Organomineral Fertilizers On Water Useand Quality Attributes Of Sugar Beet, Ali̇ Ünlükara

Turkish Journal of Agriculture and Forestry

Experiments were conducted under provincial conditions of Kayseri, Turkey, for two years in the growing seasons of 2014 and 2015 to determine the effects of irrigation water applications based on different sugar beet root zone depths and different organomineral fertilizers on sugar beet yield, root quality, water consumption, and water use efficiency. Two constant root depths (D1 = 0.9 m and D2 = 0.6 m) and one active water extraction root depth (D3= 0.4-0.9 m) were investigated. A mineral fertilizer (F1), an organomineral fertilizer (F2), and organic + mineral fertilizer (F3) were considered as the subtreatments in the experiments carried …

Deciphering Global Dna Variations And Embryo Sac Fertility In Autotetraploid Rice Line, Fozia Ghouri, Jiani Zhu, Hang Yu, Jinwen Wu, Faheem Shahzad Baloch, Xiangdong Liu, Muhammad Qasim Shahid

Turkish Journal of Agriculture and Forestry

Autotetraploid rice is a new germplasm resource obtained by doubling chromosomes through colchicine treatment. There have been many studies on the reproductive characteristics of autotetraploid rice, but little is known about global DNA variations and reasons for low embryo sac fertility in autotetraploid rice. Therefore, an autotetraploid rice line (T446) was used for resequencing and embryo sac fertility was observed. Whole-genome resequencing data revealed 87,229 SNPs and 11,022 InDels in the genome of T446 versus E246 (diploid rice), which had an average of 23.37 SNPs and 2.95 InDels per 100 kb. A total of 17,375 and 17,171 structural variations and …

Beyond Bird Feed: Proso Millet For Human Health And Environment, Saurav Das, Rituraj Khound, Meenakshi Santra, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Domesticated in 8000–10,000 BP in northern China, proso millet (Panicum miliaceum L.) is the best adaptive rotational crop for semiarid central High Plains of the USA, where average annual precipitation is 356–407 mm. Proso millet has multiple benefits when consumed as human food. Proso millet is rich in minerals, dietary fiber, polyphenols, vitamins and proteins. It is gluten-free and therefore, ideal for the gluten intolerant people. Proso millet contains high lecithin which supports the neural health system. It is rich in vitamins (niacin, B-complex vitamins, folic acid), minerals (P, Ca, Zn, Fe) and essential amino acids (methionine and cysteine). …

A Spatial Framework For Ex-Ante Impact Assessment Of Agricultural Technologies, José F. Andrade, Juan I. Rattalino Edreira, Andrew Farrow, Marloes P. Van Loon, Peter Q. Craufurd, Jairos Rurinda, Shamie Zingore, Jordan Chamberlin, Lieven Claessens, Julius Adewopo, Martin K. Van Ittersum, Kenneth G. Cassman, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Traditional agricultural research and extension relies on replicated field experiments, on-farm trials, and demonstration plots to evaluate and adapt agronomic technologies that aim to increase productivity, reduce risk, and protect the environment for a given biophysical and socio-economic context. To date, these efforts lack a generic and robust spatial framework for ex-ante assessment that: (i) provides strategic insight to guide decisions about the number and location of testing sites, (ii) define the target domain for scaling-out a given technology or technology package, and (iii) estimate potential impact from widespread adoption of the technology(ies) being evaluated. In this study, we developed …

Pursue Undergraduate Research Journal Volume 2 (Issue 1) 2019

Pursue: Undergraduate Research Journal

The scholarly journal, “PURSUE: Undergraduate Research Journal” (ISSN 2473-6201), provides undergraduates an avenue to publish their original research abstracts and articles in the following areas: psychology, sociology, biology, chemistry, physics, engineering, computer science, mathematics, humanities, agriculture, architecture, health, business, and education (this list is not exclusive).

The original research articles included in this journal are peer reviewed and selected by the journal’s Editorial Board. The review process allows undergraduate researchers to receive feedback from notable scientists in their field of study and teach them about the publication process. Publication of their work will not only inform the scientific community; it …

The Genome Of Broomcorn Millet, Changsong Zou, Leiting Li, Daisuke Miki, Delin Li, Qiming Tang, Lihong Xiao, Santosh Rajput, Ping Deng, Li Peng, Wei Jia, Ru Huang, Meiling Zhang, Yidan Sun, Jiamin Hu, Xing Fu, Patrick S. Schnable, Yuxiao Chang, Feng Li, Hui Zhang, Baili Feng, Xinguang Zhu, Renyi Liu, James C. Schnable, Jian-Kang Zhu, Heng Zhang

Department of Agronomy and Horticulture: Faculty Publications

Broomcorn millet (Panicum miliaceum L.) is the most water-efficient cereal and one of the earliest domesticated plants. Here we report its high-quality, chromosome-scale genome assembly using a combination of short-read sequencing, single-molecule real-time sequencing, Hi-C, and a high-density genetic map. Phylogenetic analyses reveal two sets of homologous chromosomes that may have merged ~5.6 million years ago, both of which exhibit strong synteny with other grass species. Broomcorn millet contains 55,930 proteincoding genes and 339 microRNA genes. We find Paniceae-specific expansion in several subfamilies of the BTB (broad complex/tramtrack/bric-a-brac) subunit of ubiquitin E3 ligases, suggesting enhanced regulation of protein dynamics …

Applicability Of The Dual Isotopes Δ15n And Δ18o To Identify Nitrate In Groundwater Beneath Irrigated Cropland, R. F. Spalding, A. J. Hirsh, M. E. Exner, N. A. Little, K. L. Kloppenborg

Department of Agronomy and Horticulture: Faculty Publications

Identification of the nitrate sources that adversely impact groundwater quality is a necessary first step in the control of this major worldwide pollutant. The impact of nitrate leachate from urea-ammonium nitrate (UAN) (50% urea-N, 25% ammonium-N, 25% nitrate-N) fertilizer, whose use has increased dramatically in the last three decades largely because it can be applied through sprinkler irrigation systems to corn in all growth stages, is investigated. The dual isotopes δ¹⁵N_NO3 and δ¹⁸O_NO3 were measured in groundwater samples from 39 irrigation wells in two intensively sprinkler-irrigated, corn-growing areas of Nebraska with nitrate-contaminated (N …

Evaluation Of Heritage Beans In West Virginia, Lewis Jett, Silas Childs

Midwest Vegetable Trial Reports

No abstract provided.

Plant Spacing Influence On Sweet Corn Yield And Quality, Ron G. Goldy, Kyle Ferrantella

Midwest Vegetable Trial Reports

No abstract provided.

Irrigated Pinto Bean Crop Stress And Yield Assessment Using Ground Based Low Altitude Remote Sensing Technology, Rakesh Ranjan, Abhilash K. Chandel, Lav R. Khot, Haitham Y. Bahlol, Jianfeng Zhou, Rick A. Boydston, Phillip N. Miklas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The pinto bean is one of widely consumed legume crop that constitutes over 42% of the U.S dry bean production. However, limited studies have been conducted in past to assess its quantitative and qualitative yield potentials. Emerging remote sensing technologies can help in such assessment. Therefore, this study evaluates the role of ground-based multispectral imagery derived vegetation indices (VIs) for irrigated the pinto bean stress and yield assessments. Studied were eight cultivars of the pinto bean grown under conventional and strip tillage treatments and irrigated at 52% and 100% of required evapotranspiration. Imagery data was acquired using a five-band multispectral …

Effects Of Solids Removal On Water Quality And Channel Catfish Production In A Biofloc Technology Production System, Bartholomew W. Green, Kevin K. Schrader, Matthew Mcentire

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Total suspended solids control was evaluated in a channel catfish (Ictalurus punctatus) biofloc technology production system. Settling chamber flow rates were 0.9 (LO) or 2.9 (HI) L/min to reduce total suspended solids to 300 mg/L; solids were not removed from control tanks. Channel catfish yields (7.6–8.7 kg/m3) were not affected significantly, but control fish were skewed toward smaller size classes. Control treatment channel catfish tolerated 1,410 mg/L total suspended solids without adverse effects. LO- and HI-treatment fillet geosmin concentrations were high enough to be designated as off-flavor. Water quality results suggested that nitrification was affected by solids removal.

Development Of Chlorophyll-Meter-Index-Based Dynamic Models For Evaluation Of High-Yield Japonica Rice Production In Yangtze River Reaches, Ke Zhang, Xiaojun Liu, Syed Tahir Ata-Ul-Karim, Songyang Li, Brian Krienke, Songyang Li, Qiang Cao, Yan Zhu, Weixing Cao, Yongchao Tian

Department of Agronomy and Horticulture: Faculty Publications

Accurate estimation of the nitrogen (N) spatial distribution of rice (Oryza sativa L.) is imperative when it is sought to maintain regional and global carbon balances. We systematically evaluated the normalized differences of the soil and plant analysis development (SPAD) index (the normalized difference SPAD indexes, NDSIs) between the upper (the first and second leaves from the top), and lower (the third and fourth leaves from the top) leaves of Japonica rice. Four multi-location, multi-N rate (0–390 kg ha^-1) field experiments were conducted using seven Japonica rice cultivars (9915, 27123, Wuxiangjing14, Wunyunjing19, Wunyunjing24, Liangyou9, and Yongyou8). Growth …

Engineering Linear, Branched-Chain Triterpene Metabolism In Monocots, Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J. Sato, Zhengxiang Ge, Thomas E. Clemente, Joseph Chappell

Department of Agronomy and Horticulture: Faculty Publications

Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to yield farnesyl diphosphate (FPP), catalyzed by FPP synthase (FPS). Squalene synthase (SQS) condenses two molecules of FPP to generate the symmetrical product squalene, the first committed precursor to sterols and most other triterpenes. In the green algae Botryococcus braunii, two FPP molecules can also be condensed in an asymmetric manner yielding the more highly branched triterpene, botryococcene. Botryococcene …

Spatial Variability Of Soil Properties Under Different Land Use In The Dang District Of Nepal, Dinesh Panday, Roshan Babu Ojha, Devraj Chalise, Saurav Das, Bikesh Twanabasu

Department of Agronomy and Horticulture: Faculty Publications

Increased nutrient mining, soil erosion, and limited nutrient management has led to declines in soil quality and reduced productivity in many parts of Nepal. A study was conducted in the eastern part of the Dang district of Nepal in 2015 to assess the variability of selected soil properties of three different land use types (agricultural, agroforestry, and grassland) and to map their spatial distribution. A total of 120 soil samples were collected from 0–15 cm depth and analyzed for soil fertility parameters: pH, organic matter (OM), nitrogen (N), phosphorus (P), potassium (K), boron (B), and zinc (Zn). Results revealed that …

Flue Gas Desulfurization Gypsum As Soil Amendment, Dinesh Panday, Richard Ferguson, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Flue gas desulfurization (FGD) gypsum is one of the by-products of a coal-fired power generation plant. Coal is the world’s most abundant and widely distributed fossil fuel. After natural gas, coal is the second primary source of energy to generate electricity globally (more than 25%) and remains a key component of the fuel mix for power generation to meet electricity demand in most of the developing countries. The U.S., China and India are the top coal producers and consumers (for production of electricity from coal sources) in the world (OECD/IEA, 2014; IEA, 2016). However, in the U.S., its contribution to …

Expression Of The Arabidopsis Wrinkled 1 Transcription Factor Leads To Higher Accumulation Of Palmitate In Soybean Seed, Pamela A. Vogel, Shen Bayon De Noyer, Hyunwoo Park, Hanh Nguyen, Lili Hou, Taity Changa, Hoang Le Khang, Ozan N. Ciftci, Tong Wang, Edgar B. Cahoon, Thomas Elmo Clemente

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max [L.] Merr.) is a commodity crop highly valued for its protein and oil content. The high percentage of polyunsaturated fatty acids in soybean oil results in low oxidative stability, which is a key parameter for usage in baking, high temperature frying applications, and affects shelf life of packaged products containing soybean oil. Introduction of a seed-specific expression cassette carrying the Arabidopsis transcription factor WRINKLED1 (AtWRI1) into soybean, led to seed oil with levels of palmitate up to approximately 20%. Stacking of the AtWRI1 transgenic allele with a transgenic locus harbouring the mangosteen steroyl-ACP thioesterase (GmFatA) resulted …

A World Of Cobenefits: Solving The Global Nitrogen Challenge, Benjamin Z. Houlton, Maya Almaraz, Viney Aneja, Amy T. Austin, Edith Bai, Kenneth G. Cassman, Jana E. Compton, Eric A. Davidson, Jan Willem Erisman, James N. Galloway, Baojing Gu, Guolin Yao, Luiz A. Martinelli, Kate Scow, William H. Schlesinger, Thomas P. Tomich, Chao Wang, Xin Zhang

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen is a critical component of the economy, food security, and planetary health. Many of the world's sustainability targets hinge on global nitrogen solutions, which, in turn, contribute lasting benefits for (i) world hunger; (ii) soil, air, and water quality; (iii) climate change mitigation; and (iv) biodiversity conservation. Balancing the projected rise in agricultural nitrogen demands while achieving these 21st century ideals will require policies to coordinate solutions among technologies, consumer choice, and socioeconomic transformation.

The Mitochondrial Genome Of Eleusine Indica And Characterization Of Gene Content Within Poaceae, Nathan D. Hall, Hui Zhang, Jeffrey P. Mower, Joseph Scott Mcelroy, Leslie R. Goertzen

Department of Agronomy and Horticulture: Faculty Publications

Plant mitochondrial (mt) genome assembly provides baseline data on size, structure, and gene content, but resolving the sequence of these large and complex organelle genomes remains challenging due to fragmentation, frequent recombination, and transfers of DNA from neighboring plastids. Themt genome for Eleusine indica (Poaceae: goosegrass) is comprehensibly analyzed here, providing key reference data for an economically significant invasive species that is also the maternal parent of the allotetraploid crop Finger millet (Eleusine coracana). The assembled E. indica genome contains 33 protein coding genes, 6 rRNA subunits, 24 tRNA, 8 large repetitive regions 15 kb of transposable elements across a …

United States Midwest Soil And Weather Conditions Influence Anaerobic Potentially Mineralizable Nitrogen, Jason D. Clark, Kristen S. Veum, Fabian G. Fernandez, James J. Camberato, Paul R. Carter, Richard B. Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, Carrie A. M. Laboski, Emerson D. Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen provided to crops through mineralization is an important factor in N management guidelines. Understanding of the interactive effects of soil and weather conditions on N mineralization needs to be improved. Relationships between anaerobic potentially mineralizable N (PMN_an) and soil and weather conditions were evaluated under the contrasting climates of eight US Midwestern states. Soil was sampled (0–30 cm) for PMN_an analysis before pre-plant N application (PP_0N) and at the V5 development stage from the pre-plant 0 (V5_0N) and 180 kg N ha−1 (V5_180N) rates and incubated for 7, 14, and …

Agronomic And Quality Response Of Hard Red Spring Wheat Genotypes To Management Systems In South Dakota, Jonathan Kleinjan

Electronic Theses and Dissertations

Intensive cereals management techniques such as multiple fungicide applications and in-season split N applications have been used to successfully improve wheat yields in Europe and in some winter wheat production areas of the USA. However, research on the effects of these management practices and their interaction with genotypes is limited in the hard red spring wheat (HRSW) production areas of the USA. The objectives of this study were to: (i) compare management treatments and (ii) quantify any interaction effects between management and genotype on the agronomic characteristics and relevant flour and dough properties of locally-adapted HRSW genotypes. A randomized complete …

Farm To Label: A Critique Of Consumer Activism In The Sustainable Food Movement, Olivia Whitener

Pomona Senior Theses

“Local,” “organic,” “natural,” and “Fairtrade” are just several of the many claims adorning the food products that line grocery store shelves. These promises of environmental sustainability and social responsibility are pillars of the “good food revolution” sweeping the nation as consumers demand alternatives to the products of the industrial food system. Green consumerism, the premise that consumer demand for environmentally sustainable goods will bring about ecologically beneficial outcomes, is at the heart of the sustainable food movement. This thesis takes a critical look at the operation of green consumerism in the food system. It explores the ideology and shortcomings of …

Attainment And Maintenance Of Pubertal Cyclicity May Predict High A4 Cows With Reduced Fertility, Sarah Nafziger

Department of Animal Science: Dissertations, Theses, and Student Research

Puberty attainment was investigated in heifers. Blood samples were collected from weaning until breeding during 6 years from heifers born in 2012-2017. Plasma progesterone concentrations ≥ 1 ng/ml were used to indicate cyclicity, and heifers were categorized into one of four puberty groups using SAS: 1) Early heifers reached puberty before March 12 and cycled continuously during the sampling period, 2) Typical heifers reached puberty on or after March 12 and cycled continuously, 3) Start-Stop heifers had at least one occurrence of progesterone ≥ 1 ng/ml but discontinued cycling during the sampling period, and 4) Non-Cycling heifers had no occurrence …

Growing South Dakota (Winter 2019), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This is issue contains the South Dakota Agricultural Experiment Station 2018 Annual Report, and the SDSU Extension 2018 Annual Report.

[Page] 2 Setting Into role as Dean
[Page] 4 Raven Precision Ag Center Groundbreaking Ceremony
[Page] 10 Collaborating for Research-Based Solutions
[Page] 12 From the SDSU Extension Director
[Page] 14 Empowering South Dakotans Through Training and Certifications
[Page] 18 Mental Health and Opioid Education
[Page] 21 Science of Agriculture
[Page] 23 Community Gardens Bring Hope to Pine Ridge Indian Reservation
[Page] 26 Energize! Conference
[Page} 28 Disaster Preparedness Training
[Page] 30 Bush Foundation Change Network
[Page] 32 SDSU to Participate …

Determinants Of Maternal Health Care Utilization In The Philippines: Implications On Information Dissemination Strategies, Nancy M. Landicho

Journal of Public Affairs and Development

From 2000 to 2017, maternal deaths in the Philippines considerably decreased. With COVID-19, the mechanisms to ensure safe pregnancy and childbirth for mothers have been greatly challenged. To find possible means by which women can access health care services remotely, the study looked into the factors affecting Filipino women’s utilization of maternal health care services using the 2017 Philippine National Demographic and Health Survey. Place of residence and employment status significantly affect the frequency by which women read newspaper or magazine, listen to the radio, and access the internet. Wealth status is the sole socio-demographic characteristic that has significant relationship …

Production And Marketing Of Indigenous Cash Crops: The Experience Of Women Farmer-Entrepreneurs In Mountain Province, Philippines, Joyce D. Cuyangoan

Journal of Public Affairs and Development

This study focused on the production factors and marketing mix of five indigenous cash crops cultivated by 67 women farmer- entrepreneurs in the municipalities of Tadian and Lower Bauko in Mountain Province, Philippines. The study used descriptive statistics and applied profit analysis in the analysis of its findings. Results of this study show that the factors that limit the respondents’ production are limited access to transportation, rainfed irrigation, and limited access to agricultural inputs and loans. In marketing their produce, it was found that the respondents have limited knowledge on processing, packaging, labeling, promoting and financial record keeping. They can …