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Host Restriction Factors In The Replication Of Tombusviruses: From Rna Helicases To Nucleocytoplasmic Shuttling, Cheng-Yu Wu

Theses and Dissertations--Plant Pathology

Positive-stranded (+)RNA viruses replicate inside cells and depend on many cellular factors to complete their infection cycle. In the meanwhile, (+)RNA viruses face the host innate immunity, such as cell-intrinsic restriction factors that could block virus replication.

Firstly, I have established that the plant DDX17-like RH30 DEAD-box helicase conducts strong inhibitory function on tombusvirus replication when expressed in plants and yeast surrogate host. This study demonstrates that RH30 blocks the assembly of viral replicase complex, the activation of RNA-dependent RNA polymerase function of p92pol and viral RNA template recruitment.

In addition, the features rendering the abundant plant DEAD-box helicases either …

Southeast South Dakota Experiment Farm Annual Progress Report, 2019, Southeast South Dakota Research Farm

Agricultural Experiment Station and Research Farm Annual Reports

This is an annual report of the research program at the Southeast South Dakota Research Farm in cooperation with South Dakota Agricultural Experiment Station and the SDSU College of Agriculture, Food, and Environmental Sciences and has special significance for those engaged in agriculture and the agriculturally related businesses in the ten county area of Southeast South Dakota. The results shown are not necessarily complete or conclusive. Interpretations given are tentative because additional data resulting from continuation of these experiments may result in conclusions different from those based on any one year

Microalgae And Zinc Source Supplementation Of Broiler Breeder Diets Affects Broiler Breeder Skeletal Development And Reproduction With Transgenerational Impacts On Offspring Performance And Skeletal Characteristics, Marquisha A. Paul

Theses and Dissertations--Animal and Food Sciences

Leg problems and disorders are of major concern for the poultry industry and animal welfare. Previous studies suggest that it may be possible to improve skeletal characteristics through nutrition. Omega-3 fatty acids, including Docosahexaenoic acid (DHA), are essential nutrients and are known to play an important role in bone tissue development. FORPLUS™ (Alltech, Inc.) is an unextracted, whole-cell microalgae (Aurantiochytrium limacinum CCAP 4087/2) that contains 64% fat and 16% DHA. Zinc (Zn) is a trace element and essential nutrient for growth and skeletal development. Bioplex^® Zn (Alltech, Inc.) is a Zn proteinate that has been shown to be …

Assessing The Value Of Grazed Corn Residue For Crop And Cattle Producers, Daren Redfearn, Jay Parsons, Mary Drewnoski, Marty Schmer, Rob Mitchell, James Macdonald, Jaymelynn Farney, Alexander Smart

Department of Agronomy and Horticulture: Faculty Publications

Integrated crop–livestock systems have included grazing perennial grasses during the spring and summer and corn (Zea mays L.) residues during the winter. Our objectives were to identify opportunities for expanded corn residue use through grazing and provide an economic assessment for value-added grazing. We estimated the economic value to the crop sector through grazing leased corn residue at over $95 million for Nebraska, South Dakota, Kansas, and North Dakota under current management. Additionally, gross economic value to the livestock sector in these states was estimated at greater than $191 million. Advantages for increased grazing use of corn residue include …

Climate-Smart Practices For Improvement Of Crop Yields In Mid-Hills Of Nepal, Roshan Subedi, Laxmi Dutt Bhatta, Erica Udas, Nand Kishor Agrawal, Keshab Datta Joshi, Dinesh Panday

Department of Agronomy and Horticulture: Faculty Publications

Farming in Nepal mostly represents the hill farming system with the dominance of small-holder farmers. In recent days, farmers in the country are impacted by climate change. Events of surface runoff, landslides, and soil erosions, along with changes in rainfall pattern and intensity have elevated a decline in crop productivity and soil fertility. Considering the situation, a pilot project on Resilient Mountain Village was implemented in Kavrepalanchowk district of Nepal from 2014–2016 with a participatory approach to demonstrate climate-smart practices. These practices include the application of locally prepared bio-fertilizer (named as “jholmal”), green manuring in rice (Oryza sativa L.) …

Drought Stress Tolerance In Wheat And Barley: Advances In Physiology, Breeding And Genetics Research, Ahmed Sallam, Ahmad M. Alqudah, Mona F. A. Dawood, P. Stephen Baenziger, Andreas Borner

Department of Agronomy and Horticulture: Faculty Publications

Climate change is a major threat to most of the agricultural crops grown in tropical and sub-tropical areas globally. Drought stress is one of the consequences of climate change that has a negative impact on crop growth and yield. In the past, many simulation models were proposed to predict climate change and drought occurrences, and it is extremely important to improve essential crops to meet the challenges of drought stress which limits crop productivity and production. Wheat and barley are among the most common and widely used crops due to their economic and social values. Many parts of the world …

Nebraska Farm Real Estate Market Highlights 2018-2019, Jim Jansen, Jeffrey Stokes

Extension Farm and Ranch Management News

Introduction

The Nebraska Farm Real Estate Market Highlights 2018-2019 report represents the 41st edition to the annual series. These reports provide an important insight on agricultural land market dynamics for stakeholders across Nebraska. In today’s market, where market transactions exceeding a million dollars are the norm, objective market information and analysis is more critical than ever. The focus of the report continues to provide unbiased information on agricultural land values and rental rates so industry participants can make educated and informed decisions.

This year, the February 2019 survey of nearly 125 expert-panel members from across the state provided current information …

Hydrodynamic Cavitation As In-Line Process To Control Common Dairy Sporeformers, Pratibha Chaudhary

Electronic Theses and Dissertations

Sporeforming bacteria and their endospores have the ability to survive thermal treatments and proliferate in the milk and dairy products to cause spoilages. We applied the effect of hydrodynamic cavitation in controlling these bacteria and their spores at pilot scale level. Our aim was to investigate a technology that would improve the microbial quality of milk and dairy products, and is capable of being scaled up to be adapted at industrial level. Cavitation damages the cell walls of the bacteria and their endospores (releasing dipicolinic acid, responsible for endospore resistance to extreme conditions), thereby making them susceptible to thermal treatments …

Preservice Agricultural Education Majors’ Perceptions Of Integrating Science Into Agriculture, Kasey Trocke

Electronic Theses and Dissertations

Agricultural science has seen a rise in secondary agricultural classrooms and many studies have been done with regards to integrating science into agriculture. Several recommendations have been to assist preservice agriculture education majors with science integration. Preservice agricultural education majors could influence the future of agricultural science due to their perceptions of integrating science into agriculture into the classroom. The purpose of the study was to assess preservice teachers’ perceptions of integrating science into agriculture curriculum based on the theory of planned behavior. An online survey was administered to the population of agricultural education majors at South Dakota State University. …

Sunflower Pollinators In South Dakota: An Evaluation Of Species Composition, Abundance And Influence On Yield, Mackenzie Mattern

Electronic Theses and Dissertations

In 2017 and 2018, South Dakota was the largest producer of sunflowers, Helianthus annuus L. (Asterales: Asteraceae) in the US, grossing approximately $178 million per year. In South Dakota, both confection and oilseed sunflower cultivars are grown. Despite breeding efforts to increase sunflower's ability to self-pollinate there is evidence that pollinator visitation results in increased sunflower yields. In addition, there is evidence that sunflower pollen is beneficial for pathogen resistance in various bee species, suggesting the importance of a mutualistic relationship between pollinators and sunflowers. In some areas, observed increases in sunflower yield is attributed to the presence of Apis …

Maximizing Ecosystem Services Provided To The New Oil Crop Brassica Carinata Through Landscape And Arthropod Diversity, Shane Stiles

Electronic Theses and Dissertations

Prairies, once spanning the Upper Midwest, have now largely been replaced by agriculture. The lack of resources available to pollinators in agricultural fields and the practices employed by farmers to maximize yield has led to a decline in insect and pollinator diversity. There is a need to better understand how ecosystem services provided by a diverse insect community scale to current farming practices as they relate to crop yield. We sought to explain how landscape heterogeneity relates to insect and pollinator diversity, as well as how insect diversity relates to crop yield across common farming practices. To evaluate how farming …

Parents In Urbanized Areas And Their Perception Of 4-H Youth Development Programs, Matthew Ryan Olson

Electronic Theses and Dissertations

This study examined the perceptions held by parents living in the urbanized area of Rapid City, South Dakota, to determine if artificial barriers to joining 4-H PYD programs existed. Through snowball sampling methods, nine participants were recruited to engage in semi-structured interviews. Transcripts from the interviews were coded utilizing in vivo and axial coding methods. Four themes emerged from the interviews: characteristics of youth development programs, program promotion through school flyers, barriers to joining youth development programs, and 4-H and its connection to agriculture. The major recommendation for 4-H professionals is to focus on leadership, life skills, and character development …

Letter From The Dean, Deacue Fields

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

A Message From The Associate Dean Of The Honors College And Co-Chair Of The Service Learning Initiative, Jennie Popp

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 20 2019, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Environmentally Friendly Fertilizers Can Enhance Yield And Bioactive Compounds Inchinese Cabbage (Brassica Rapa Ssp. Pekinensis), Jeongyeo Lee, Young-Hee Noh, Kun-Hyang Park, Dae-Soo Kim, Han Taek Jeong, Haeng-Soon Lee, Sung Ran Min, Hyeran Kim

Turkish Journal of Agriculture and Forestry

The objective of this study was to evaluate the performance of 3 environmentally friendly fertilizers and their effect on the growth of Chinese cabbage and its contents of bioactive compounds. The following fertilizers were applied: purslane extract, 5% sulfur, and 15% phosphoric acid with 5% calcium. A total of 9, 9, and 11 components were detected for fatty acids, carotenoids, and glucosinolates, respectively. The contents of cis-7 hexadecenoic acid, linoleic acid (C18:2n6c+t), and linolenic acid (C183n3) were generally increased, and also carotenoid amounts of violaxanthin, antheraxanthin, lutein, 13-cis-ß-carotene, α-carotene, ß-carotene, and 9-cis-ß-carotene were significantly increased by fertilizer treatments. All fertilization …

Molecular Characterization Of Apple (Malus × Domestica Borkh.) Genotypes Originatingfrom Three Complementary Conservation Strategies, Anca Butiuc, Ana Coste, Anca Farkas, Victoria Cristea, Valentina Isac, Adela Halmagyi

Turkish Journal of Agriculture and Forestry

Apple (Malus × domestica Borkh.) genotypes originating from different plant collections (field collection, in vitro plant collections undergoing or not undergoing cryopreservation) were screened and characterized by SSR markers. Shoot tips excised from plants grown in vitro were successfully cryopreserved by encapsulation-dehydration. The highest regrowth frequency (69%, cultivar Goldrush) of cryopreserved apices was achieved after 24 h of osmoprotection in 0.5 M sucrose, 3 h of desiccation, and 24% water content of alginate beads. No differences in morphological characteristics including shoot length and number and length of roots were observed between controls and plants recovered after cryopreservation. SSR markers were …

Louisiana Agriculture Winter, 2019, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Undoing Gender: An Analysis Of How Women Communicate Within The Agricultural Industry, Shala R. Larson

Electronic Theses and Dissertations

In recent history, women have entered more business-like positions in the agricultural industry. This thesis seeks to understand how women in agriculture describe their workplace communication and whether they feel they are part of a muted group. Twelve women were interviewed regarding their experiences as women in agriculture and were asked whether they feel valued in their positions and how they communicate with their male co-workers. Specifically, interviewees were asked if they ever felt the need to alter their communication to complement, emulate, or otherwise adjust to masculine communication styles. The majority of women interviewed reported having to strategically choose …

Diversifying Cornfields By Interseeding Cover Crops: Practical Implications And The Response Of Invertebrate Communities, Michael Bredeson

Electronic Theses and Dissertations

The lack of suitable habitat conditions for beneficial organisms in simplified agroecosystems leads to unstable invertebrate communities and overreliance on chemical control of herbivores. It is possible to manage pest populations without agrichemicals by manipulating farmland so that herbivores are impaired by plant-driven bottom-up and enemy-driven top-down antagonisms. Interseeding cover crops between established crop rows is a method used by farmers to improve habitat suitability for natural enemies and hinder host-finding, feeding and movement by herbivores. Here I address three important research gaps related to interseeding cover crops. A calcium carbonate seed coating, used to improve seed-broadcasting efficiency, was tested …

Evaluating And Improving Consumer Perceptions Of The Agriculture Industry, Katelyn Berndt

Schultz-Werth Award Papers

Consumer perceptions of the agriculture industry are increasingly important as alternative labeling and non-conventional options become more common. Consumers are seeking a more dominant role in the conversation about their food. This study explores how the agriculture industry can respond in a way that improves consumer perceptions and builds trust. The study also seeks to answer the question of whether country of birth or background affects consumer perceptions of agriculture. The research model includes a QuestionPro survey completed by students at South Dakota State University, representing consumers in the Millennial generation who are more likely to respond positively to transparent …

Capacity To Elicit Cytotoxic Cd8 T Cell Activity Against Mycobacterium Avium Subsp. Paratuberculosis Is Retained In A Vaccine Candidate 35 Kda Peptide Modified For Expression In Mammalian Cells, Valentina Franceschi, Asmaa H. Mahmoud, Gaber S. Abdellrazeq, Giulia Tebaldi, Francesca Macchi, Luca Russo, Lindsay M. Fry, Mahmoud M. Elnaggar, John Bannantine, Kun-Taek Park, Victoria Hulubei, Sandro Cavirani, William C. Davis, Gaetano Donofrio

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Studies focused on development of an attenuated vaccine against Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of paratuberculosis (Ptb) in cattle and other species, revealed that deletion of relA, a global gene regulator, abrogates the ability of Map to establish a persistent infection. In the absence of relA, cattle develop CD8 cytotoxic T cells (CTL) with the ability to kill intracellular bacteria. Analysis of the recall response to a relA mutant, Map/􏰀relA, with cells from a vaccinated steer demonstrated that a 35-kDa membrane peptide (MMP) is one of the targets of the response. This observation suggested that it might …

Phenotypes Of Macrophages Present In The Intestine Are Impacted By Stage Of Disease In Cattle Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Caitlin J. Jenvey, Adrienne L. Shircliff, J. P. Bannantine, Judith R. Stabel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Macrophages play an important role in the host immune response to Mycobacterium avium subsp. paratuberculosis (MAP) infection, however, MAP is able to disrupt normal macro- phage functions to avoid destruction. It is unclear whether the phenotypes of macrophages present in the target tissue play a role in the inability to clear MAP infection. The aim of this study was to identify macrophage phenotypes (host defense or resolution and repair) present within the bovine ileum of naturally infected cattle, as well as to ascertain abundance of each macrophage phenotype present during different stages of MAP infection. Immunofluo- rescent (IF) labeling was …

Predicting Spatial Risk Of Wolf-Cattle Encounters On Rugged And Extensive Grazing Lands, Patrick R. Clark, Joe Chigbrow, Douglas E. Johnson, John Williams, Larry L. Larson, Tyanne Roland

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cattle grazing lands in the mountainous western United States are rugged, complex, and extensive. Terrain, vegetation, and other landscape features vary greatly across space. Risk of wolf-cattle encounters and potential for depredation loss certainly differ spatially as consequence of this variability. Yet, our understanding of this spatial risk is quite poor and this knowledge gap severely hampers our abilities to manage wolf-livestock interactions and mitigate conflicts. During 2009-2011, a research study was conducted at four study areas (USFS cattle grazing allotments) in western Idaho to evaluate and predict risk of wolf-cattle encounters. Each year, a random sample of 10 lactating …

Characterization Of Ethanol Extracted Cell Wall Components Of Mycobacterium Avium Subsp. Paratuberculosis, John P. Bannantinea, Ashutosh Wadhwa, Judith R. Stabel, Shigetoshi Eda

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Antigens extracted using ethanol (EtOH) and incorporated in the EtOH vortex ELISA (EVELISA) test have previously shown high specificity and sensitivity for detecting Mycobacterium avium subspecies paratuberculosis (Map) and M. bovis infections in cattle. The objective of this study is to define the components present in the EtOH extract. We show that this extract is composed of lipid, carbohydrate, and proteins on the surface of the bacilli, and that EtOH removes the outer layer structure of Map which comprise these elements. To identify proteins, polyclonal antibodies to the EtOH prep were produced and used to screen a Map genomic expression …

Effects Of Moisture Content, Internode Region, And Oblique Angle On The Mechanicalproperties Of Sainfoin Stem, Mustafa Gökalp Boydaş, Mustafa Çomakli, Bahadir Sayinci, Mazhar Kara

Turkish Journal of Agriculture and Forestry

The effects of moisture content, internode region, and oblique angle on several mechanical properties of sainfoin stem were determined. The selected mechanical properties used were the shearing stress, specific shearing energy, bending stress, and modulus of elasticity when bending. The experiments were carried out with wet basis (WB) moisture contents of 71.76%, 45.57%, and 25.57%, at the third and second internode regions from the bottom up to the top, and 2 oblique angles (0° and 28°). The research results showed that the shearing stress of sainfoin stem increased as the moisture content increased, but decreased towards the upper internode of …

Nebline, January 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

2018 Extension highlights

Benefit to people

Teaching, growing and making a difference

36,520 youth engaged in 4-H programs

Extension’s volunteers worth nearly $700,000

New Year, new you: Nutrition tips to a healthier you

RECIPE OF THE MONTH

Pesticide applicator 2019 training dates

Ag Estate and Succession Planning workshop, Jan. 23 & March 5

Successful Farmer Series

Upcoming green industry conferences

Extension’s 2019 Master Gardener trainings, two sites available

Garden Guide Things to Do This Month

Sticky situation: Using glue boards to monitor pests

HEART OF 4-H Volunteer Award: Susan Bulling

IT’S TIME TO RE-ENROLL IN 4-H!

Meet 2018–2019 4-H Council …

2019 Annual Report, Ben Bohall, John Erixson

Nebraska Forest Service: Publications

No abstract provided.

Perceptions Of Wyoming Agriculture Teachers About The Importance Of Sae’S, Casey Styvar

Electronic Theses and Dissertations

Agricultural education in the United States after the Smith Hughes Act has always included a component of the home-based project method, which now is known as a supervised agricultural experience program (SAEP). This method of instruction teaches and provides students with a hands-on learning experience inside and outside of the classroom. The benefits that students receive with this form of the curriculum are second to none. Agriculture educators realize the benefits of SAE programs and how their students expand and grow in their education and skills from having an SAE. Due to changes in student and teacher culture, it is …

Greenhouse Aquaponics: Custom Aquaponic Systems At Home, Jesse L. Blanchard, David G. Hyatt, Jennie Popp, Leah English

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Taking advantage of inherent natural systems, aquaponic practices hold the potential to serve as an educational, sustainable, and profitable hobby for home gardeners facing common constraints such as temperature, space, and pests. The goal of this research was to assess the feasibility of implementing a small scale (4,542-liter) home -based aquaponic system in a small (48.768 square meter) greenhouse to produce fresh produce and fish protein. System construction and maintenance costs were compared to the value of crops and fish produced to determine whether this aquaponic system is a feasible option for the home grower. It was hypothesized that this …