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Comparative Genomics Of Five Valsa Species Gives Insights On Their Pathogenicity Evolution, Guangchao Sun, Shichang Xie, Lin Tang, Chao Zhao, Mian Zhang, Lili Huang

Department of Agronomy and Horticulture: Faculty Publications

Valsa is a genus of ascomycetes within the Valsaceae family. This family includes many wood destructive pathogens such as the well known Valsa mali and Valsa pyri which cause canker diseases in fruit trees and threaten the global fruit production. Lack of genomic information of this family is impeding our understandings about their evolution and genetic basis of their pathogenicity divergence. Here, we report genome assemblies of Valsa malicola, Valsa persoonii, and Valsa sordida which represent close relatives of Valsa mali and Valsa pyri with different host preferences. Comparative genomics analysis revealed that segmental rearrangements, inversions, and translocations …

Comparative Genome Analysis Of Plant Ascomycete Fungal Pathogens With Different Lifestyles Reveals Distinctive Virulence Strategies, Yansu Wang, Jie Wu, Jiacheng Yan, Ming Guo, Lei Xu, Liping Hou, Quan Zou

Department of Agronomy and Horticulture: Faculty Publications

Background: Pathogens have evolved diverse lifestyles and adopted pivotal new roles in both natural ecosystems and human environments. However, the molecular mechanisms underlying their adaptation to new lifestyles are obscure. Comparative genomics was adopted to determine distinct strategies of plant ascomycete fungal pathogens with different lifestyles and to elucidate their distinctive virulence strategies. Results: We found that plant ascomycete biotrophs exhibited lower gene gain and loss events and loss of CAZyme-encoding genes involved in plant cell wall degradation and biosynthesis gene clusters for the production of secondary metabolites in the genome. Comparison with the candidate effectome detected distinctive variations between …

Biobased Sprayable Mulch Films Suppressed Annual Weeds In Vegetable Crops, Eliott Gloeb, Sibel Irmak, Loren Isom, John L. Lindquist, Samuel E. Wortman

Department of Agronomy and Horticulture: Faculty Publications

Biobased sprayable mulch (BSM) films are a potential alternative to herbicides, polyethylene plastic mulch film, and hand weeding for specialty crops. We developed a series of BSM films using locally available biomaterials [including corn (Zea mays) starch, glycerol, keratin hydrolysate, corn gluten meal, corn zein, eggshells, and isolated soy (Glycine max) protein] and tested their effects on weeds and crop yield during a total of seven greenhouse or field trials between 2017 and 2019 in Nebraska, USA. Application rates of BSM films applied in pots (greenhouse), planting holes in plastic film (field), or bed tops (field) …

Etiology And Epidemiology Of Mini-Ring In Ultradwarf Bermudagrass Putting Greens, Lukas Dant

All Dissertations

Mini-ring is a disease in ultradwarf bermudagrass (UDBG) [Cynodon dactylon (L.) Pers. × C. transvaalensis (Burtt-Davy)] putting greens caused by Waitea zeae (Voorhees) J.A. Crouch & Cubeta, (formerly Rhizoctonia zeae). Symptoms typically resemble frog-eye patches that are 10 to 40 cm in diameter with a bronze to orange outer ring and green center. In the southeastern United States, mini-ring symptoms appear in late-summer and generally persist until UDBG dormancy in late-fall. Mini-ring is often problematic in UDBG when nitrogen (N) fertility is reduced to manage organic matter production and improve putting green performance and perceived green speed. While …

Sustainability And Health Impacts Of Pulse Crops In The United States Using Life Cycle Assessment, Prathamesh A. Bandekar

Graduate Theses and Dissertations

Environmental sustainability and human health impact of pulses produced and consumed in the United States was assessed using life cycle assessment (LCA). The study included three objectives 1) to estimate environmental impact of current production and consumption practices in the United States using attributional LCA; 2) to estimate environmental and human health impact of iso-caloric diets containing varying amounts of pulses using Hybrid-LCA and Combined Nutritional and Environmental-LCA (CONE-LCA); and 3) to estimate environmental impact of increased demand for pulses using consequential LCA. Scope of the study varied for each objective with system boundary encompassing cradle-to-grave activities for objective 1 …

Insights On The Behavior Of Nano-Copper In The Agroecosystem: Mycorrhizal Associations With Spearmint (Mentha Spicata), Suzanne Annette Apodaca

Open Access Theses & Dissertations

Nanotechnology offers significant potential benefits to our society, including the agriculture sector. With the advancement of nano-enabled agrochemicals towards sustainable and efficient agricultural practices, it is essential to address environmental issues associated with the use of nanoscale materials. The same properties that give promise to applications of nanotechnology in modern agriculture could have unintended consequences on ecosystem dynamics. A point of concern for risk management is the impact of engineered nanomaterials (ENMs) to beneficial microbial communities, which support a variety of ecosystem services.

Use of copper (Cu) products in agriculture are based on their abundance, role as a micronutrient, and …

Comparative Metabolites Profiling Of Sesamum Indicum Seeds In Context Of Their Origin, Processing And Biological Activity Using Bioinformatics Tools, Bishoy Fayez Abib

Theses and Dissertations

Sesame (Sesamum indicum), is a significant oilseed crop with great economical and nutritional value, encompassing a plethora of primary and secondary metabolites that exhibit pharmacological and therapeutic merits. The present study, using untargeted comparative metabolomics, aims to authenticate and classify sesame seed accessions from different countries of origin i.e., Egypt, Sudan and Nigeria, based on their metabolite variations and in relation to their biological activity and nutritional value such as mineral content. Also, to further assess the impact of processing on sesame’s nutritional value and biological activity using the industrial production of sesame paste as a case study. To achieve …

A Non-Destructive Approach For Measuring Rice Panicle-Level Photosynthetic Responses Using 3d-Image Reconstruction, Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Harel Bacher4, Zvi Peleg, Paul E. Staswick, Jason Hupp, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Background: Our understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impact on grain yield. This is primarily due to the lack of a suitable gas exchange methodology for panicles and non-destructive methods to accurately determine panicle surface area.

Results: To address these challenges, we have developed a custom panicle gas exchange cylinder compatible with the LiCor 6800 Infra-red Gas Analyzer. Accurate surface area measurements were determined using 3D panicle imaging to normalize the panicle-level photosynthetic measurements. We observed differential responses in …

Bison Movements Change With Weather: Implications For Their Continued Conservation In The Anthropocene, Nicholas A. Mcmillan, Samuel D. Fuhlendorf, Barney Luttbeg, Laura E. Goodman, Craig A. Davis, Brady W. Allred, Robert G. Hamilton

Department of Agronomy and Horticulture: Faculty Publications

Animal movement patterns are affected by complex interactions between biotic and abiotic landscape conditions, and these patterns are being altered by weather variability associated with a changing climate. Some animals, like the American plains bison (Bison bison L.; hereafter, plains bison), are considered keystone species, thus their response to weather variability may alter ecosystem structure and biodiversity patterns. Many movement studies of plains bison and other ungulates have focused on point-pattern analyses (e.g., resource-selection) that have provided information about where these animals move, but information about when or why these animals move is limited. For example, information surrounding the …

The Genome Of Orychophragmus Violaceus Provides Genomic Insights Into The Evolution Of Brassicaceaepolyploidizationandits Distinct Traits, Kang Zhang, Yinqing Yang, Xin Zhang, Lingkui Zhang, Yu Fu, Zhongwei Guo, Shumin Chen, Jian Wu, James C. Schnable, Keke Yi, Xiaowu Wang, Feng Cheng

Department of Agronomy and Horticulture: Faculty Publications

Orychophragmus violaceus, referred to as ‘‘eryuelan’’ (February orchid) in China, is an early-flowering ornamental plant. The high oil content and abundance of unsaturated fatty acids in O. violaceus seeds make it a potential high-quality oilseed crop. Here, we generated a whole-genome assembly for O. violaceus using Nanopore and Hi-C sequencing technologies. The assembled genome of O. violaceus was ~1.3 Gb in size, with 12 pairs of chromosomes. Through investigation of ancestral genome evolution, we determined that the genome of O. violaceus experienced a tetraploidization event from a diploid progenitor with the translocated proto-Calepineae karyotype. Comparisons between the reconstructed subgenomes …

Novel Single-Nucleotide Variants For Morpho-Physiological Traits Involved In Enhancing Drought Stress Tolerance In Barley, Ibrahim S. Elbasyoni, Shamseldeen Eltaher, Sabah Morsy, Alsayed M. Mashaheet, Ahmed M. Abdallah, Heba G. Ali, Samah A. Mariey, P. Stephen Baenziger, Katherine Anna Frels

Department of Agronomy and Horticulture: Faculty Publications

Barley (Hordeum vulgare L.) thrives in the arid and semi-arid regions of the world; never-theless, it suffers large grain yield losses due to drought stress. A panel of 426 lines of barley was evaluated in Egypt under deficit (DI) and full irrigation (FI) during the 2019 and 2020 growing seasons. Observations were recorded on the number of days to flowering (NDF), total chlorophyll content (CH), canopy temperature (CAN), grain filling duration (GFD), plant height (PH), and grain yield (Yield) under DI and FI. The lines were genotyped using the 9K Infinium iSelect single nucleotide polymorphisms (SNP) genotyping platform, which …

Effects Of Sowing Date And Phosphorus Fertiliser Application On Winter Survival Of Lucerne Cv. Aohan In The Northern Semi-Arid Region Of China, Z. L. Wang, Q. Zh. Sun, Y. W. Wang, Zh. Y. Li, Sh. F. Zhao

IGC Proceedings (1977-2023)

In the northern semi-arid region of China, winter survival is always a limiting factor for lucerne production, because low temperatures and a dry climate in winter (Zhou et al., 1993; Ma, 2000; Sun & Gui, 2001; Sun et al., 2003). An experiment was conducted to find an appropriate sowing date and P application rate in order to improve lucerne winter survival.

Ear Emergence Of Different Grass Species Under Finnish Growing Conditions, M. Niskanen, O. Niemeläinen, L. Jauhiainen

IGC Proceedings (1977-2023)

Timothy is the most commonly cultivated grass species in Finland. Swards cultivated for silage of hay are of pure timothy or timothy is the dominant species of the mixture. Successful timing of harvesting of the primary growth is very critical in Northern latitudes where the stand develops very rapidly at daylengths of above 18 hours with a daily mean temperature close to 20 °C. The primary growth has to be harvested within a very short period to obtain a yield of high and uniform quality. Short harvesting periods require high capacity harvest machinery which lead to high costs. If it …

Reasons For The Premature Decline In Astragalus Adsurgens Stands In Kerqin Sandy Land, Q. Zh. Sun, Z. L. Wang, J. G. Han, Y. W. Wang, G. R. Liu

IGC Proceedings (1977-2023)

Diseases partly account for reductions in Astragalus adsurgens, stand longevity. The effect of some cultural practices on the control of pests and diseases have been reported (Hou, 1986; Nan, 1996), but few reports have detailed the relationship among soil fertiliser status, diseases and premature stand decline. This study was conducted to investigate these relationships in order to extend the longevity of Astragalus adsurgens stands.

The Influence Of Fertiliser Application To Strip-Sown Grasslands On Herbage Production And Quality, A. Kohoutek, P. Komárek, V. Odstrčilová, P. Nerušil

IGC Proceedings (1977-2023)

To increase the productivity of dairy cattle in the Czech Republic requires an improvement in herbage quality and an increase in the net energy of herbage. The decrease in cattle numbers by 50 % and the expansion of the grassland area both result in a surplus of feedstuffs of low quality. Introduction of strip-seeding of legumes and grasses into grasslands in interaction with fertilisation provides a possible solution to this problem.

Range-Based Livestock Production In Turkmenistan, R. H. Behnke, G. Davidson

IGC Proceedings (1977-2023)

Turkmenistan retains a centralized system of livestock production in which many critical assets are owned by the state. Though technically in the temperate zone, the country's climate is harsh and unstable. Groundwater resources are unevenly distributed, leaving many potential grazing areas seasonally inaccessible due to lack of drinking water for livestock. This paper summarizes the results of a three-year study of rangelands, livestock production, flock economics and land tenure at two study sites, one in central and the other in eastern Turkmenistan. The results of this study suggest that pastoral communities in Turkmenistan have coped remarkably well with the institutional …

Green Dairy, A Project For Environmental Friendly And Sustainable Dairy Systems In The Atlantic Area, H. Chambaut, A. Pflimlin, C. Raison

IGC Proceedings (1977-2023)

Green Dairy is an European Interreg III B project which runs over 3 years. It brings together 10 research and development partners from 5 countries and 11 regions of the Atlantic Area which range from Scotland to Portugal. It aims to provide a better understanding of the impact of intensive dairy systems on the quality of the environment in order to develop ways of improving practices in the different regions. This is expected to encourage more rapid responses to the problems of deteriorating water and air quality and a more appropriate response within or between regional contexts that could be …

Rangeland Ecological Management Counter-Measures Study Of Xinjiang, H. X. Cui, J. Li, S. Asiya, J. L. Zhang, Jialin

IGC Proceedings (1977-2023)

Xinjiang is located in the hinterland of the Asian-Euro continental area and far from the ocean. It is surrounded by mountains and forms a physiognomic landscape of two basins located in three mountains. This results in an extremely droughty continental climate and a harsh environment in the Temperate Zone. However, the geographical environment is diverse in the Xinjiang Autonomous region, the three high mountains and complicated geographical structure results in changes in ecological conditions with altitude and a diverse ecological environment. Because of the drought characteristics the desert area has developed different types of vegetation, and the complexity of the …

Management Options To Reduce N-Losses From Ploughed Grass-Clover, J. De Wit, G. J. Van Der Burgt, N. Van Eekeren

IGC Proceedings (1977-2023)

Nitrate (NO₃ -) leaching from grassland can be kept at acceptable levels, but is often high after ploughing for grassland renewal or for silage maize/grain production. In on-farm research with several organic farmers, management options are being explored to save scarce organic manure and to reduce N-losses.

Mechanical Aeration And Liquid Dairy Manure: Application Impacts On Grassland Runoff Water Quality And Yield, T. J. Basden, S. B. Shah, J. L. Miller

IGC Proceedings (1977-2023)

Wet weather on heavy soils reduces oxygen availability in the root zone and reduces forage yields. Mechanical aeration can improve forage yield in these soil conditions. Research has shown that under certain conditions, mechanical aeration can increase yield by improving drainage and aeration (Davies et al., 1989); aeration can also increase depression, storage and infiltration thus reducing surface runoff and improving nutrient distribution in the root zone. Aeration on sloping, fertilised grassland can provide environmental (Douglas et al., 1995) and agronomic benefits. The objectives of this study were to evaluate the runoff water quality and agronomic impacts of …

Nitrogen Dynamics Following The Break-Up Of Grassland On Three Different Sandy Soils, Manfred Kayser, K. Seidel, J. Müller

IGC Proceedings (1977-2023)

Nitrogen (N) is accumulated under grassland depending on factors such as soil type, management, and fertiliser input. Break-up of grassland stimulates mineralisation of organic N and may lead to increased soil mineral N and leaching losses (Lloyd, 1992). The objective of this study was to find out how site factors, e.g. soil, previous management and sward age, the following crop and the new level of N fertiliser affect the amount of inorganic N in autumn and the preceding mineralisation processes when grassland is ploughed in spring.

Fine Colloids ‘Carry’ Diffuse Water Contaminants From Grasslands, P. M. Haygarth, A. L. Heathwaite

IGC Proceedings (1977-2023)

The transport of diffuse pollutants from grassland has traditionally been described by the operationally defined threshold of greater, or smaller than a nominated membrane filter size. Most commonly this has been a 0.45 μm threshold to define ‘solute’ and ‘particulate’ transport. In this paper we shall use phosphorus (P) to help provide an example of the importance of colloid-facilitated transport.

Leaching Losses Of N, P And K From Grazed Legume Based Swards: Some Preliminary Results, E. R. Dixon, A. C. Stone, D. Scholefield, D. J. Hatch

IGC Proceedings (1977-2023)

There is increasing interest in sustainable agricultural systems because of environmental concerns. Animal production which utilises mixed grass and legume swards could be an effective measure in increasing the efficiency of nutrient utilisation, and investigation into different legume species is necessary. Leaching losses of N, P and K from 3 legume species under grazing by sheep were measured using Teflon-coated suction cups. The experiment took place on the UK site of the EU-funded, multi-site experiment – LEGGRAZE.

Soil Aggregate Dynamics, Particulate Organic Matter And Phosphate Under Dryland And Irrigated Pasture, J. T. Scott, L. M. Condron, R. W. Mcdowell

IGC Proceedings (1977-2023)

Soil aggregate formation and turnover affects the rate of occlusion or release of soil organic matter and therefore the availability for mineralisation or stabilisation of soil carbon (C) and phosphorus (P). Furthermore, differences in soil type, management and the quantity and quality of organic inputs can affect aggregate turnover rates (Six et al., 2000). Under pastoral farming the ratio of coarse particulate organic matter (inter-POM) inside macroaggregates but outside microaggregates to fine POM (intra-POM) within microaggregates may provide an indication of physical processes influencing mineralisation and stabilisation of soil C and organic P (Po). Our aim was to determine …

Fire And Nutrient Cycling In Shortgrass Steppe Of The Southern Great Plains, Usa, P. L. Ford, C. S. White

IGC Proceedings (1977-2023)

Fire in semi-arid grasslands releases nutrients bound up in organic matter and accelerates the rate of decomposition in the soil. This research experimentally tested effects of season and frequency of fire on nutrient cycling dynamics in shortgrass steppe. The objective was to identify if fire treatments have the ability to increase potential grassland productivity relative to untreated ‘reference condition’ grassland. Many such studies focus on short-term, direct effects of fire. However, this study is part of a long-term, 18-year study examining both direct, and indirect effects of fire in the growing vs. dormant season at return intervals of 3, 6 …

Maximising Slurry Crop Available Nitrogen Utilisation In Grassland Systems, J. R. Williams, E. Sagoo, B. J. Chambers, J. Laws, D. R. Chadwick

IGC Proceedings (1977-2023)

In the UK, approximately 90 million tonnes of animal manure containing ca 450,000 tonnes of nitrogen (N) are recycled to agricultural land each year. The efficient utilisation of manure N can save farmers money and reduce diffuse air (ammonia) and water (nitrate) pollution. For slurries, bandspreading techniques (e.g. trailing shoe and trailing hose) can improve N utilisation by reducing ammonia volatilisation losses compared with conventional broadcast applications. They also provide increased spreading opportunities in spring/summer as slurry is placed in a band on the soil surface limiting herbage contamination, which can reduce the need to apply slurry in the autumn/early …

Phosphorus Transfer To River Water From Grassland Catchments In Ireland, H. Tunney, P. Jordan, G. Kiely, R. Moles, G. Morgan, P. Byrne, W. Menary, K. Daly

IGC Proceedings (1977-2023)

In Ireland it is estimated that at least half of phosphorus (P) loss to water is from agricultural sources and National and European Union policy and legislation aim at reducing phosphorus (P) loss to water in order to reduce eutrophication. In Ireland, the average soil test P (STP) levels increased ten-fold, from less than 1 to over 8 mg Morgan P per l soil over the past 50 years, reflecting increased P inputs in fertiliser and animal feed. One of the main objectives of this three-year research programme, started in 2001, was to investigate P loss to water in grassland …

Denitrification Under Pastures On Permeable Soils Helps Protect Ground Water Quality, M. P. Russelle, B. A. Browne, N. B. Turyk, B. Pearson

IGC Proceedings (1977-2023)

Pastures have been implicated in ground water contamination by nitrate, especially in humid regions with thin or sandy soils (Stout et al., 2000). Significant losses can occur even under low N input, because available N from excreta patches often exceeds plant uptake capacity. Lack of evidence that appreciable nitrate leaching was occurring in established Midwestern USA pastures led us to test the hypothesis that denitrification was preventing or remediating nitrate loading. Higher denitrification rates have been found in the relatively limited number of trials since Ball & Ryden (1984) first reported the significance of this process in pastures.

Assessment Of Nitrogen Nutrition Status Of Grasses Under Water Deficit And Recovery, V. G. Dugo, Jean-Louis Durand, F. Gastal

IGC Proceedings (1977-2023)

Grasslands are rarely irrigated. They are therefore systematically submitted to more or less severe water deficits: as well as mineral deficiencies, water scarcity often also results in a reduction of nitrogen (N) status. Although identified some time ago, qualitatively, the interaction with N still remains difficult to take into account in quantitative analyses of crop physiology under water deficits. This paper illustrates how the nitrogen (N) status of the crop changes under water deficits. A N nutrition index (INN) was defined as the ratio of the actual N concentration of forage with the theoretical N concentration under optimal conditions, the …

Improving Nutrient Supply Of Grassland Soil, G. Füleky, M. Orbán

IGC Proceedings (1977-2023)

In the Southern corner of the Eastern Carpathian Mountains is located the hilly region of Barcaság. The total agricultural area is 180 thousand ha of which 90 thousand ha is grassland. The maximum yield of natural grassland does not exceed 1.2 t ha^-1 hay. The aim of this work is to find appropriate fertiliser and liming rates to increase the hay yield and improve the soil nutrient supply.