Digital Commons Network^™

Intsormil 2007 Annual Report, John M. Yohe, Kimberly Christiansen, Joan Frederick

INTSORMIL Impacts and Bulletins

The 2007 INTSORMIL Six Year Report presents the progress and notable achievements by the Sorghum/Millet CRSP during the period of July 1, 2001 - June 30, 2007. These results are an outcome of partnerships between scientists at seven U.S. Land Grant Universities (Kansas State University, Mississippi State University, Ohio State University, University of Nebraska, Purdue University, Texas A&M University and West Texas A&M University), scientists of the Agricultural Research Service of the U.S. Department of Agriculture at Tifton, Georgia and the National Agricultural Research Systems (NARS) and National Universities in nineteen countries in Central America, West Africa, East Africa and …

Enhancing Fertiliser Use Efficiency For Transplanted Vegetables, Dennis Phillips, Aileen Reid, Gavin D'Adhemar

Horticulture research reports

Horticulture Australia (HAL) Project No. VG04018.

Project VG 04018 ‘Enhancing fertiliser use efficiency for transplanted vegetables’ showed that spraying highly concentrated solutions containing nitrogen over newly transplanted vegetable seedlings for between 14 and 21 days after planting can increase marketable yields by as much as 300% compared to other methods of fertiliser application. Crops that this method has been successfully tested on in this study by the W.A. Department of Agriculture and Food include iceberg and Cos lettuce, broccoli, cauliflower, Chinese cabbage (wombok), celery and cabbage.

The research was conducted on virgin sandy soils of the Swan Coastal Plain near …

Australian Temperate Strawberry Breeding Program (Western Australia) Selection Of New And Advanced Short Day Breeding Lines And Cultivars In Western Australia - 2006/2007, Aileen Reid, Dennis Phillips, Hal

Horticulture research reports

Thirty-two ‘Stage 4’ numbered selections (2004 crosses) from the Australian Temperate Strawberry Breeding Program were evaluated in unreplicated plots on a commercial property in Wanneroo for their potential under WA conditions. Seven of these have been selected as worthy of further testing. The priority selection among these was 04- 021-263, and the other six included 04-021-467, 04-037-102, 04-021-241, 04-021-172, 04-021-475 and 04-029-211.

In addition, 21 advanced numbered selections (Stages 5, 6 and 7) were also evaluated on the same grower’s property. Three of those were substantially larger plots and a later planting date. Of the first group of 18 selections, …

Ua66/5/2 Newsletter, Wku Department Of Agriculture

WKU Administration Documents

Newsletter regarding programs, events, students and alumni of the WKU Agriculture department.

Highly Variable Patterns Of Linkage Disequilibrium In Multiple Soybean Populations, D. L. Hyten, Ik-Young Choi, Qijian Song, Randy C. Shoemaker, Randall L. Nelson, Jose M. Costa, James E. Specht, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Prospects for utilizing whole-genome association analysis in autogamous plant populations appear promising due to the reported high levels of linkage disequilibrium (LD). To determine the optimal strategies for implementing association analysis in soybean (Glycine max L. Merr.), we analyzed the structure of LD in three regions of the genome varying in length from 336 to 574 kb. This analysis was conducted in four distinct groups of soybean germplasm: 26 accessions of the wild ancestor of soybean (Glycine soja Seib. et Zucc.); 52 Asian G. max Landraces, the immediate results of domestication from G. soja; 17 Asian Landrace …

Evects Of In Vivo Exposure To Deet On Blood Feeding Behavior And Fecundity In Anopheles Quadrimaculatus (Diptera: Culicidae), Rui-De Xue, Arshad Ali, Donald R. Barnard

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study determined the effects of contact with DEET on guinea pig skin on mortality, probing time, blood feeding rate, engorgement time, and fecundity responses in female Anopheles quadrimaculatus Say. Exposure, in this manner, to 10% DEET (in ethanol) for 5min, resulted in 98% mortality in mosquitoes after 24 h. The median probing time (PT₅₀) required by females, when exposed to 0.1%, 1.0%, and 10% DEET, was significantly (P< 0.0001) longer (12.5, 12.1, and 19.1 s, respectively) than the 6.8 s required by females to probe ethanol-treated skin (control). Similarly, mean blood feeding rates in populations of females exposed to 1.0% DEET for ≤5min (14.4%) was 6x lower (P <0.001) (85.5%) than in females exposed to ethanol-treated skin, whereas the mean engorgement time on skin treated with 1.0% DEET (66.3 s) was significantly shorter (P<0.0001) than for females feeding on the control guinea pigs (105.9 s). The mean number of mature oöcytes per female (fecundity) in treatment (1.0% DEET) and control mosquitoes was not significantly different. The responses to DEET observed in this study suggest that repeated exposure of female A. quadrimaculatus populations to this repellent, in laboratory bioassays, could result in confounding of toxicant and repellent effects and inaccurate estimates of DEET repellency.

Fish And Wildlife Benefits Associated With Wetland Establishment Practices, Charles A. Rewa

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Efforts to establish wetlands through restoration and creation actions have increased in recent decades in response to regulatory and voluntary incentive programs. This paper summarizes the findings of studies conducted to document fish and wildlife response to these practices. The majority of published studies describe bird response to wetland restoration, with most reporting bird communities in restored wetlands to be similar to those of natural reference wetlands. Studies also indicate that invertebrates and amphibians generally respond quickly to and colonize newly established wetland habitats. Key factors reported as correlated with wildlife species richness include wetland size, availability of nearby wetlands …

Effect Of Drying On Phosphorus Distribution In Poultry Manure, Heidi W. Dail, Zhongqi He, M. Susan Erich, C. Wayne Honeycutt

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Laboratory drying may alter manure phosphorus (P) distribution. The effects of freeze, air (22 °C), and oven (65 °C) drying on sequentially fractioned poultry manure P were examined. Higher drying temperatures resulted in lower percentage of dry matter. Increased H₂O- and decreased sodium bicarbonate (NaHCO₃)-extractable P with drying provided evidence that drying increases poultry manure P solubility. Labile fractions were predominantly inorganic P (P_i), whereas sodium hydroxide (NaOH) and hydrochloric acid (HCl) fractions had significant amounts of organic P (P_o). Drying altered H₂O- and NaHCO₃-extractable P_i but …

Efficiency Of Gene Silencing In Arabidopsis : Direct Inverted Repeats Vs. Transitive Rnai Vectors, Sergei A. Filichkin, Stephen P. Difazio, Amy M. Brunner, John M. Davis, Zamin K. Yang, Udaya C. Kalluri, R. S. Arias, Elizabeth Etherington, Gerald A. Tuskan, Steven H. Strauss

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We investigated the efficiency of RNA interference (RNAi) in Arabidopsis using transitive and homologous inverted repeat (hIR) vectors. hIR constructs carry self-complementary intron-spliced fragments of the target gene whereas transitive vectors have the target sequence fragment adjacent to an intron-spliced, inverted repeat of heterologous origin. Both transitive and hIR constructs facilitated specific and heritable silencing in the three genes studied (AP1 , ETTIN and TTG1). Both types of vectors produced a phenotypic series that phenocopied reduction of function mutants for the respective target gene. The hIR yielded up to fourfold higher proportions of events with strongly manifested reduction …

Pollination Biology Of A Disjunct Population Of The Endangered Sandhills Endemic Penstemon Haydenii S. Wats. (Scrophulariaceae) In Wyoming, Usa, V. J. Tepedino, T. L. Griswold, T.R. Toler, B. A. Bradley, J. L. Hawk

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We studied the breeding system and flower visitors of the endangered plant, Penstemon haydenii, at several south-central Wyoming, USA occurrences. In agreement with earlier studies of the species 300 km to the east in Nebraska, we found Wyoming plants to be self-incompatible and pollinator-dependent for sexual reproduction. Flower visitors were several species of native bees in the families Apidae (particularly bumblebees), Halictidae (small sweat bees), and Megachilidae (especially in the genus Osmia); and the masarid wasp Pseudomasaris vespoides. Especially important was Osmia brevis, an abundant megachilid bee, and one of only two species (the sweat bee …

Growth And Pubertal Development Of F1 Bulls From Hereford, Angus, Norwegian Red, Swedish Red And White, Friesian, And Wagyu Sires, E. Casas, D. Lunstra, Larry V. Cundiff, Johny Joe Ford

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The objective of the study was to characterize body growth, testicular development, and puberty from 8 to 14 mo of age in bulls (n = 120) produced by mating sires from Hereford, Angus, Norwegian Red, Swedish Red and White, Friesian, and Wagyu breeds to MARC III (¹⁄₄ Hereford, ¹⁄₄ Angus, ¹⁄₄ Red Poll, and ¹⁄₄ Pinzgauer) cows. Traits evaluated were birth weight, weaning weight (at 215 d), yearling weight, ADG from 8 to 14 mo of age, paired testicular volume growth from 8 to 14 mo of age, age at puberty (determined by production of 50 × 10⁶ sperm …

Mitigation Of Shallow Groundwater Nitrate In A Poorly Drained Riparian Area And Adjacent Cropland, Jennifer H. Davis, Stephen M. Griffith, William R. Horwath, Jeffrey J. Steiner, David D. Myrold

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Riparian ecosystems, through their unique position in the agricultural landscape and ability to influence nutrient cycles, can potentially reduce NO₃ loading to surface and ground waters. The purpose of this study was to determine the fate of NO₃ in shallow groundwater moving along a lateral flowpath from a grass seed cropping system through an undisturbed mixed-species herbaceous riparian area. Soil A (30–45 cm) and C horizon (135–150 cm) NO₃, dissolved oxygen, and nitrous oxide concentrations were significantly higher in the cropping system than the adjacent riparian area. Nitrate concentrations in both horizons of the riparian soil …

The Original Usda-Ars Experimental Watersheds In Texas And Ohio: Contributions From The Past And Visions For The Future, R. D. Harmel, J. V. Bonta, C. W. Richardson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The USDA Soil Conservation Service (USDA‐SCS) realized the importance of understanding hydrologic processes on agricultural fields and watersheds in the mid‐1930s. Based on this realization, the research program of the Hydrologic Division of SCS established three experimental watersheds across the U.S. to analyze the impact of landuse practices on soil erosion, flood events, water resources, and the agricultural economy. Two of the original watersheds remain in operation today within the USDA Agricultural Research Service (USDA‐ARS): the Blacklands Experimental Watershed (now the Grassland, Soil and Water Research Laboratory) near Riesel, Texas, and the North Appalachian Experimental Watershed near Coshocton, Ohio. These …

Dry Weight And Nitrogen Content Of Chickpea And Winter Wheat Grown In Pots For Three Rotations, James E. Bidlack, Charles T. Mackown, Srinivas C. Rao

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Chickpea [Cicer arietinum (L.)] cultivars ‘ICCV-2’ and ‘Sarah’ were studied along with a control, multistrain, TAL 1148, and TAL 480 Bradyrhizobium strains to determine the effect(s) of cultivar and inoculum on dry weight (DW) and nitrogen (N) content of the legume, as well as soil mineral N, DW, and N content of wheat [Triticum aestivum (L.) emend. Thell.] in a continuous wheat-legume rotation. Chickpeas were planted during the summer and harvested in the fall of 1992, 1993, and 1994. Vegetative growth from chickpeas was incorporated into the soil prior to wheat planting, and soil cores were taken at …

Comparison Of Ground-Based Remote Sensors For Evaluation Of Corn Biomass Affected By Nitrogen Stress, Soon-Dal Hong, James Schepers, Dennis D. Francis, Michael R. Schlemmer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The nondestructive determination of plant biomass is not possible; however, crop-canopy sensors that determine the normalized difference vegetation index have the potential to estimate living biomass, which is indicative of plant vigor and stress. Pot experiments using sand culture were conducted in 2002 and 2003 under greenhouse conditions to evaluate the effect of nitrogen (N) deficiency on corn biomass and reflectance. Stress was imposed by implementing six to eight levels of N in Hoagland’s nutrient solution. Canopy reflectance measurements made at three growth stages with a variety of handheld spectral sensors (active and passive) were closely correlated with dry weight …

Design And Testing Of A Closed, Stirring Respirometer For Measuring Oxygen Consumption Of Channel Catfish Eggs, Eugene L. Torrans

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A closed, stirring respirometer was designed to agitate small egg mass samples of channel catfish Ictalurus punctatus while measuring oxygen consumption by the eggs. Egg mass samples ranging in size from 15 to 46 g were placed on a screen platform above a magnetic stir bar in the respirometer; the degree of agitation was controlled with a magnetic stir plate. Dissolved oxygen (DO) concentration and temperature were measured with a luminescent DO sensor and meter. The respirometer was used to determine routine metabolic rate and limiting oxygen concentration (the presumed hypometabolic transition zone) on egg mass samples from 10 different …

Fertility Restoration Of The Sorghum A3 Male-Sterile Cytoplasm Through A Sporophytic Mechanism Derived From Sudangrass, Hoang V. Tang, Jeffrey F. Pedersen, Christine D. Chase, Daryl R. Pring

Department of Agronomy and Horticulture: Faculty Publications

Fertility restoration of sorghum [Sorghum bicolor (L.) Moench] lines carrying the IS1112C (A3 group) male-sterile cytoplasm has been documented as a two-gene gametophytic mechanism involving complementary action of restoring alleles designated Rf3 and Rf4, as derived from IS1112C. Fertility restoration capability has also been reported from sudangrass (S. bicolor subsp. drummondii) populations. We describe characteristics of a fertility restoration system derived from sudangrass, in which male-sterile individuals were observed at high frequency in backcross and F₂-F₃ segregating populations. Segregation analyses were consistent with a sporophytic restoration system involving two complementary genes. Pollen iodine …

Technological Change And The Design Of Plant Variety Protection Regimes, Mark D. Janis, Stephen Smith

Articles by Maurer Faculty

No abstract provided.

C3–C4 Composition And Prior Carbon Dioxide Treatment Regulate The Response Of Grassland Carbon And Water Fluxes To Carbon Dioxide, H. W. Polley, W. A. Dugas, P. C. Mielnick, H. B. Johnson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

During May, July and October 2000, we measured the effects of temporarily increasing or decreasing CO₂ concentration by 150–200 μmol mol⁻¹ on daytime net ecosystem CO₂ exchange (NEE) and water flux (evapotranspiration, ET) of C₃–C₄ grassland in central Texas, USA that had been exposed for three growing seasons to a CO₂ gradient from 200 to 560 μmol mol⁻¹. Grassland grown at subambient CO₂ (< 365 μmol mol⁻¹) was exposed for 2 days to an elevated CO₂ gradient (> 365 μmol mol⁻¹). Grassland grown at elevated CO₂ was …

Landholder Information For The Safe Use And Management Of 1080, Renee Donovan, David Goodall

Biosecurity published reports

This manual outlines your roles and responsibilities for using and managing 1080. In particular this manual outlines the essential information you need to know in regard to:

• The legislative requirements for 1080 control in Western Australia
• The properties and mode of action of 1080
• How to lodge an application to lay 1080 baits How to safely store and transport 1080
• The procedures and notification required to carry out a baiting proposal
• Personal safety, identifying signs of poisoning & effecting appropriate first aid treatment
• Your responsibilities for notifying DAFWA and the Police of any accidents or incidents
• Methods for evaluating the …

Publishing Ltd Whole-Plant Mineral Partitioning Throughout The Life Cycle In Arabidopsis Thaliana Ecotypes Columbia, Landsberg Erecta, Cape Verde Islands, And The Mutant Line Ysl1ysl3, Brian M. Waters, Michael A. Grusak

Department of Agronomy and Horticulture: Faculty Publications

• Minimal information exists on whole-plant dynamics of mineral flow through Arabidopsis thaliana or on the source tissues responsible for mineral export to developing seeds. Understanding these phenomena in a model plant could help in the development of nutritionally enhanced crop cultivars. • A whole-plant partitioning study, using sequential harvests, was conducted to characterize growth and mineral concentrations and contents of rosettes, cauline leaves, stems, immature fruit, mature fruit hulls, and seeds of three WT lines (Col-0, Ler, and Cvi) and one mutant line (Col-0::ysl1ysl3). • Shoot mineral content increased throughout the life cycle for all minerals, although tissue-specific mineral …

A Soybean Transcript Map: Gene Distribution, Haplotype And Single-Nucleotide Polymorphism Analysis, Ik-Young Choi, D. L. Hyten, Lakshmi K. Matukumalli, Qijian Song, Julian M. Chaky, Charles V. Quigley, Kevin Chase, K. Gordon Lark, Robert S. Reiter, Mun-Sup Yoon, Eun-Young Hwang, Seung-In Yi, Nevin D. Young, Randy C. Shoemaker, Curtis P. Van Tassell, James E. Specht, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

The first genetic transcript map of the soybean genome was created by mapping one SNP in each of 1141 genes in one or more of three recombinant inbred line mapping populations, thus providing a picture of the distribution of genic sequences across the mapped portion of the genome. Singlenucleotide polymorphisms (SNPs) were discovered via the resequencing of sequence-tagged sites (STSs) developed from expressed sequence tag (EST) sequence. From an initial set of 9459 polymerase chain reaction primer sets designed to a diverse set of genes, 4240 STSs were amplified and sequenced in each of six diverse soybean genotypes. In the …

Mycotoxin Occurrence And Aspergillus Flavus Soil Propagules In A Corn And Cotton Glyphosate-Resistant Cropping Systems, K. N. Reddy, H. K. Abbas, R. M. Zablotowicz, C. A. Abel, C. H. Koger

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The effects of cotton–corn rotation and glyphosate use on levels of soil-borne Aspergillus flavus, aflatoxin and fumonisin contamination in corn and cotton seed were determined during 2002–2005 in Stoneville, Mississippi (USA). There were four rotation systems (continuous cotton, continuous corn, cotton–corn and corn–cotton) for both glyphosate-resistant (GR) and non-GR cultivars–herbicide system arranged in a randomized complete block design with four replications. Aspergillus flavus populations in surface (5-cm depth) soil, sampled before planting (March/April), mid-season (June) and after harvest (September), ranged from 1.47 to 2.99 log (10) cfu g^-1 soil in the four rotation systems. Propagules of A. flavus …

First Report Of Yersinia Ruckeri Biotype 2 In The Usa, Cova R. Arias, Oscar Olivares-Fuster, Karl Hayden, Craig A. Shoemaker, John M. Grizzle, Phillip H. Klesius

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A polyphasic characterization of atypical isolates of Yersinia ruckeri (causative agent of enteric redmouth disease in trout) obtained from hatchery-reared brown trout Salmo trutta in South Carolina was performed. The Y. ruckeri isolates were biochemically and genetically distinct from reference cultures, including the type strain, but were unequivocally ascribed to the species Y. ruckeri, based on API 20E, VITEK, fatty acid methyl ester profiles, and 16S rRNA gene sequencing analysis. These isolates were nonmotile and unable to hydrolyze Tween 20/80 and were therefore classified as Y. ruckeri biotype 2. Genetic fingerprint typing of the isolates via enterobacterial repetitive intergenic …

Mineralization Of Nitrogen In Soils Amended With Dairy Manure As Affected By Wetting/Drying Cycles, D. B. Watts, H. A. Torbert, S. A. Prior

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Interest in manure management and its effects on nitrogen (N) mineralization has increased in recent years. The focus of this research was to investigate the N-mineralization rates of different soil types in Coastal Plain soils and compare them to a soil from Illinois. Soils with and without dairy composted manure addition were subjected to different wetting/drying cycles [constant moisture at 60% waterfilled pore space (WFPS) and cycling moisture from 60 to 30% WFPS] under laboratory conditions at three different temperatures (11 °C, 18 °C, and 25 °C). Samples were collected from three different soil types: Catlin (Mollisols), Bama (Ultisols), and …

Non-Composted Municipal Solid Waste Processing Byproduct Effect On Soil Reclamation, H. Allen Torbert, Dick L. Gebhart, Ryan R. Busby, Kenneth N. Potter, Deborah R. Curtain

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A new garbage processing technology has been developed that sterilizes and separates inorganic and organic components of municipal solid waste. The non-composted byproduct of this process, Fluff ®, has the potential to be utilized as a soil amendment to improve soil conditions in highly degraded soils. A study was initiated to evaluate Fluff as a soil amendment for establishing native grasses on disturbed US Army training lands. The Fluff was incorporated into a sandy loam soil at Fort Benning Military Reservation, GA on two sites: a moderately degraded and a highly degraded soil. The Fluff was incorporated at rates of …

Alkaline Phosphatase Reactivity In The Vagina And Uterovaginal Junctionsperm-Storage Tubules Of Turkeys In Egg Production: Implications Forsperm Storage, M. R. Bakst, V. Akuffo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

1. Currently there remains contradictory intortliation on the localisation and possible roleof alkaline phosphatase (AP) in the chicken and Japanese quail oviducts.

2.Using turkeys with a hard-shelled egg in their uteri, vaginal and uterovaginal junction mucosae werestretched and fixed as whole mounts prior to the Imistochemical localisation of All activity.

3.Scattered All reactive cells were observed in the vaginal and uteiovaginal junction surface epitheliaand intense All reactivity of the sperm-storage tubule (SST) epithelium, localised to its apical border.

4.We suggest that such All in hens in egg production may reflect cell dif.ftreiitiation andproliferation in the vagina and SST and possibly …

Adrenomedullin (Am) And Adrenomedullin Binding Protein (Am-Bp) In The Bovine Mammary Gland And Milk: Effects Of Stage Of Lactation And Experimental Intramammary E. Coli Infection, Ted H. Elsasser, Anthony V. Capuco, Thomas J. Caperna, Alfredo Martínez, Frank Cuttitta, Stanislaw Kahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Adrenomedullin (AM) has been characterized as an endogenous tissue survival factor and modulator of many inflammatory processes. Because of the increased susceptibility of the mammary gland to infection during the time surrounding parturition in the cow, we investigated how milk and tissue content of AM and its binding protein (AM-BP) might be affected by the stage of lactation and the udder health status. Milk and mammary biopsy samples were obtained from Holstein cows 21 days prior to and at various times after calving to represent the dry period and early and midstages of lactation. Additional cows received an intramammary challenge …

Identification And Characterization Of Aquaporin-9 (Aqp9) In Porcine Hepatic Tissue And Hepatocytes In Monolayer Culture, Thomas J. Caperna, Amy E. Shannon, Mark P. Richards, Wesley M. Garrett, Neil C. Talbot

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aquaporins (AQPs) are members of a large family of integral membrane proteins involved in the rapid movement of water and neutral solutes across cell membranes. In this study, we have prepared an affinity-purified porcine-specific polyclonal antiserum to AQP9 and have investigated the distribution and expression of AQP9 in pig liver tissue and in hepatocytes in primary culture. Immunocytochemical analysis showed that AQP9 was primarily localized in the membrane structures of hepatocytes and was not associated with intrahepatic bile ducts or blood vessels. Western blot analysis indicated that AQP9 ranged in apparent molecular mass between 27 and 38 kD in whole …

Isolation And Characterization Of Porcine Visceral Endoderm Cell Lines Derived From In Vivo 11-Day Blastocysts, Neil C. Talbot, Le Ann Blomberg, Ayesha Mahmood, Thomas J. Caperna, Wesley M. Garrett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two porcine cell lines of yolk-sac visceral endoderm, designated as PE-1 and PE-2, were derived from in vivo 11-d porcine blastocysts that were either ovoid (PE-1) or at the early tubular stage of elongation (PE-2). Primary and secondary culture of the cell lines was done on STO feeder cells. The PE-1 and PE-2 cells morphologically resembled visceral endoderm previously cultured from in vivo-derived ovine and equine blastocysts and from in vitro-derived bovine blastocysts. Analysis of the PE-1- and PE-2-conditioned medium by 2D-gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flightmass spectrometry demonstrated that they produced serum proteins. Reverse transcriptase polymerase chain …