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Corn Rootworm Areawide Pest Management In The Midwestern Usa, Laurence D. Chandler, James R. Coppedge, C. Richard Edwards, Jon J. Tollefson, Gerald R. Wilde, Robert M. Faust

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

For many maize (Zea mays) producers across the Midwestern USA, as well as in parts of the northern and southern plains, the corn rootworm complex (Diabrotica spp.; Coleoptera: Chrysomelidae) has represented one of the greatest challenges to efficient, quality maize-grain production over the past 50-60 years. Three species of corn rootworm are particularly troublesome: the western corn rootworm, Diabrotica virgifera virgifera, the northern corn rootworm, D. barberi and the Mexican corn rootworm, D. virgifera zeae; all have consistently been important economic pests of maize (Metcalf, 1986b). Prior to the introduction of transgenic maize varieties designed …

Central And Peripheral Administration Of Kisspeptin Activates Gonadotropin But Not Somatotropin Secretion In Prepubertal Gilts, Clay A. Lents, Neely L. Heidorn, C. Richard Barb, J. Joe Ford

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

It is well established that kisspeptin signaling is necessary for the onset of puberty in laboratory animals. However, the role that kisspeptin may have in regulating puberty in large domestic animals is unknown.We tested the hypothesis that either central or peripheral infusion of kisspeptin would stimulate gonadotropin and GH secretion in prepubertal gilts. In experiment 1, prepubertal gilts were fitted with i.c.v. cannula and indwelling jugular catheters. Animals were randomly assigned to receive 0, 10, or 100 μg kisspeptin in saline. In experiment 2, prepubertal gilts, fitted with indwelling jugular catheters, randomly received 0, 1, 2.5, or 5 mg kisspeptin …

Improvement Of Perennial Forage Species As Feedstock For Bioenergy, William Anderson, Michael Casler, Brian S. Baldwin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Both native and non-native forage species other than switchgrass are less commonly considered as potential lignocellulosic feedstocks for bioenergy in the United States. These species hold potential as bioenergy feedstocks because of the experience and infrastructure that is already in place for management and harvest, and in certain areas of the country they have greater yield potential than switchgrass or other feedstocks. The forage grasses consist of temperate cool-season (most commonly C3) grasses as well as tropical or sub-tropical warm-season (C4) grasses. Some legume species may also play a significant role in supplying useful bioenergy feedstocks. Most have been researched …

The Artiodactyl Apobec3 Innate Immune Repertoire Shows Evidence For A Multi-Functional Domain Organization That Existed In The Ancestor Of Placental Mammals, Rebecca Larue, Stefán Jónsson, Kevin Silverstein, Mathieu Lajoie, Denis Bertrand, Nadia El-Mabrouk, Isidro Hötzel, Valgerdur Andrésdóttir, Timothy Smith, Reuben Harris

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: APOBEC3 (A3) proteins deaminate DNA cytosines and block the replication of retroviruses and retrotransposons. Each A3 gene encodes a protein with one or two conserved zinccoordinating
motifs (Z1, Z2 or Z3). The presence of one A3 gene in mice (Z2–Z3) and seven in humans, A3A-H (Z1a, Z2a-Z1b, Z2b, Z2c-Z2d, Z2e-Z2f, Z2g-Z1c, Z3), suggests extraordinary evolutionary flexibility. To gain insights into the mechanism and timing of A3 gene expansion and into the functional modularity of these genes, we analyzed the genomic sequences, expressed cDNAs and activities of the full A3 repertoire of three artiodactyl lineages: sheep, cattle and pigs.
Results: …

Susceptibility Of Eggs And Adult Fecundity Of The Lesser Grain Borer, Rhyzopertha Dominica, Exposed To Methoprene, Y. Chanbang, F. H. Arthur, G. E. Wilde, J. E. Throne, Bh. Subramanyam

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A series of tests were conducted to determine the susceptibility of eggs and neonates of the lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae = Bostrychidae), exposed to the insect growth regulator, methoprene, on filter paper and on rough rice. In the first test, the hatch rate of eggs exposed on filter paper treated with methoprene at the label rate of 0.003 mg [AI] /cm² when used as a surface treatment in structures was 52.0 ± 7.3% compared to 93.0 ± 3.3% on untreated controls. In the second test, eggs were exposed to a dose-response series of 0.00003 to …

Genomic Analysis Of Fusarium Verticillioides, D. W. Brown, R. A.E. Butchko, R. H. Proctor

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fusarium verticillioides (teleomorph Gibberella moniliformis) can be either an endophyte of maize, causing no visible disease, or a pathogen-causing disease of ears, stalks, roots and seedlings. At any stage, this fungus can synthesize fumonisins, a family of mycotoxins structurally similar to the sphingolipid sphinganine. Ingestion of fumonisin-contaminated maize has been associated with a number of animal diseases, including cancer in rodents, and exposure has been correlated with human oesophageal cancer in some regions of the world, and some evidence suggests that fumonisins are a risk factor for neural tube defects. A primary goal of the authors’ laboratory is to …

Microbial Community Structure Dynamics In Ohio River Sediments During Reductive Dechlorination Of Pcbs, Andres Enrique Nunez

University of Kentucky Doctoral Dissertations

The entire stretch of the Ohio River is under fish consumption advisories due to contamination with polychlorinated biphenyls (PCBs). In this study, natural attenuation and biostimulation of PCBs and microbial communities responsible for PCB transformations were investigated in Ohio River sediments.

Natural attenuation of PCBs was negligible in sediments, which was likely attributed to low temperature conditions during most of the year, as well as low amounts of available nitrogen, phosphorus, and organic carbon. Moreover, surface sediments were relatively oxidized, as indicated by the prevalence of aerobic bacteria such as beta- Proteobacteria, alpha-Proteobacteria, Sphingobacteria, and Nitrospira in 16S rRNA sediment …

Registration Of ‘Darrell’ Wheat, A. M. H. Ibrahim, S. D. Haley, P. S. Baenziger, Y. Jin, M. A. C. Langham, J. Rickertsen, S. Kalsbeck, R. Little, J. Ingemansen, O. K. Chung, B. W. Seabourn, G. H. Bai, Ming-Shun Chen, D. V. Mcvey

Department of Agronomy and Horticulture: Faculty Publications

‘Darrell’ (Reg. No. CV-1024, PI 644224) hard red winter wheat (Triticum aestivum L.) was developed by the South Dakota State University–Agricultural Experiment Station and released in 2006 to seed producers by the South Dakota State University–Agricultural Experiment Station and the University of Nebraska Agricultural Experiment Station. Darrell was selected from the cross 2076-W12-11/‘Karl 92’ (PI 564245)//NE89526. NE89526 is an experimental line from the University of Nebraska with the pedigree ‘Lancota’ (CI 17389) selection/’Siouxland’ (PI 483469)//TX792729. 2076-W12-11 is an unreleased experimental line that originated from a population with an unidentified pedigree. Darrell was selected as an F3:4 line in 1998 …

Comparison Of Nitrogen Fertilization Methods And Rates For Subsurface Drip Irrigated Corn In The Semi-Arid Great Plains, D. D. Tarkalson, J. O. Payero

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In semi‐arid areas such as western Nebraska, interest in subsurface drip irrigation (SDI) for corn is increasing due to restricted irrigation allocations. However, crop response quantification to nitrogen (N) applications with SDI and the environmental benefits of multiple in‐season (IS) SDI N applications instead of a single early‐season (ES) surface application are lacking. The study was conducted in 2004, 2005, and 2006 at the University of Nebraska‐Lincoln West Central Research and Extension Center in North Platte, Nebraska, comparing two N application methods (IS and ES) and three N rates (128, 186, and 278 kg N ha^-1) using a …

Winter Cover Crops And Vinegar For Early-Season Weed Control In Sustainable Cotton, P. J. Moran, S. M. Greenberg

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Weeds may be suppressed by winter cover crops and the use of organic herbicides such as vinegar. Black oat (Avena strigosa) and hairy vetch (Vicia villosa) winter cover crops were planted for 2 years as part of a sustainable production system for cotton in the Lower Rio Grande Valley of Texas, and were till-killed each spring prior to cotton planting. Palmer amaranth (Amaranthus palmeri), common purslane (Portulaca oleracea), and sunflower (Helianthus annuus) were frequently-encountered winter and spring weeds. Both cover crops controlled winter weeds as well as did winter tillage …

A Landscape Perspective In Managing Vegetation For Beneficial Plant-Pestnatural Enemy Interactions: A Foundation For Areawide Pest Management, Michael J. Brewer, Takuji Noma, Norman C. Elliott

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In the USA, Europe and increasingly in other regions, cropping systems designed for high production output are significant features of the landscape. Deployment of mechanized and high-input cropping systems over the last 50 years has resulted in substantial transformation and fragmentation of major grassland, shrubland and woodland systems throughout the world. These cropping systems are typically less diverse in species composition, structure and ecological functioning than those found in the original plant community (Altieri, 2004). Decreases in plant diversity of agroecosystems (i.e. the crops themselves and surrounding remnants of the original plant system) have negatively affected ecosystem functions (Freemark, 2005). …

Synthesis And Bioassay Of Improved Mosquito Repellents Predicted From Chemical Structure, Alan R. Katritzky, Zuoquan Wang, Svetoslav Slavov, Maia Tsikolia, Dimitar Dobchev, Novruz G. Akhmedov, C. Dennis Hall, Ulrich R. Bernier, Gary G. Clark, Kenneth Linthicum

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mosquito repellency data on acylpiperidines derived from the United States Department of Agriculture archives were modeled by using molecular descriptors calculated by CODESSA PRO software. An artificial neural network model was developed for the correlation of these archival results and used to predict the repellent activity of novel compounds of similar structures. A series of 34 promising N-acylpiperidine mosquito repellent candidates (4a–4q’) were synthesized by reactions of acylbenzotriazoles 2a–2p with piperidines 3a–3f. Compounds (4a–4q’) were screened as topically applied mosquito repellents by measuring the duration of repellency after application to cloth patches worn on the arms of human volunteers. Some …

Responsive In-Season Nitrogen Management For Cereals, J.F. Shanahan, N. R. Kitchen, W. R. Raun, James S. Schepers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Current nitrogen (N) management strategies for worldwide cereal production systems are characterized by low N use efficiency (NUE), environmental contamination, and considerable ongoing debate regarding what can be done to improve N fertilizer management. Development of innovative strategies that improve NUE and minimize off-field losses is crucial to sustaining cereal-based farming. In this paper, we review the major managerial causes for low NUE, including (1) poor synchrony between fertilizer N and crop demand, (2) uniform field applications to spatially variable landscapes that commonly vary in crop N need, and (3) failure to account for temporally variable influences on crop N …

Asas Centennial Paper: Net Energy Systems For Beef Cattle – Cattles, Application, And Future Models, Calvin Ferrell, J. W. Oltjen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Development of nutritional energetics can be traced to the 1400s. Lavoisier established relationships among O₂ use, CO₂ production and heat production in the late 1700s, and the laws of thermodynamics and law of Hess were discovered during the 1840s. Those discoveries established the fundamental bases for nutritional energetics and enabled the fundamental entity ME = retained energy + heat energy to be established. Objectives became: 1) to establish relationships between gas exchange and heat energy, 2) to devise bases for evaluation of foods that could be related to energy expenditures, and 3) to establish causes of energy expenditures. …

Chapter 18. Daycent Simulated Effects Of Land Use And Climate On County Level N Loss Vectors In The Usa, S. J. Del Grosso, W. J. Parton, D. S. Ojima, C. A. Keough, T. H. Riley, A. R. Mosier

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We describe the nitrogen (N) gas (NH₃, NO_x, N₂O, N₂) emission and NO₃ leaching submodels used in the DAYCENT ecosystem model and demonstrate the ability of DAYCENT to simulate observed N₂O emission and NO₃ leaching rates for various sites representing different climate regimes, soil types, and land uses. DAYCENT simulated seven major crops, grazing lands, and potential native vegetation at the county level for the United States. At the national scale, NO₃ leaching was the major loss vector, accounting for 86%, 66%, and 56% of total N …

Chapter 15. Proven Practices And Innovative Technologies For On-Farm Crop Nitrogen Management, N. R. Kitchen, K. W. T. Goulding, J.F. Shanahan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen (N) from soil, fertilizer, and manure sources is generally inefficiently used (30-60%) in most crop production systems. As a consequence, unused inorganic N can move off crop fields and contaminate surface and groundwater resources. Local and national governments have responded with guidelines, standards, regulations, and in some cases fines when off-field losses of N have not been reduced. Along with these environmental pressures, soaring energy costs have resulted in commensurate increased costs for N fertilizers. These factors are real for crop producers and are compelling them to scrutinize their crop N management more closely than in previous decades. Numerous …

Chapter 4. Relationship Of Environmental Nitrogen Metabolism To Human Health, J. R. Follett, R. F. Follett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The need for humans to produce and consume food and other agricultural products is increasing. This need is directly related to increasing world populations, demands for goods and services, and expectations. Nitrogen (N) is contained in all of the amino acids and proteins in the foods consumed by humans. The use of N to produce food and other products is generally increasing as human needs increase. Proteins are an essential component of the human diet because, unlike plants, humans are unable to utilize more simple forms of N and rely on food sources for protein which can then be digested …

Initial Response Of Evapotranspiration From Tallgrass Prairie Vegetation To Co2 At Subambient To Elevated Concentrations, H. W. Polley, H. B. Johnson, P. A. Fay, J. Sanabria

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A series of weighing lysimeters in which tallgrass prairie species had been planted were exposed to a subambient to elevated gradient in CO₂ in a field chamber. Lysimeters with intact monoliths of three soil types were represented along the CO₂ gradient. We used regression analysis to determine how CO₂ effects on evapotranspiration per unit of soil surface area (ET_soil) and per unit of leaf area (ET_la) depended on variation in leaf area index (LAI) and diurnal changes in environmental variables during the initial 6 weeks of CO₂ treatment.

Hydraulic Core Extraction: Cutting Device For Soil–Root Studies, T. S. Kornecki, S. A. Prior, G. B. Runion, H. H. Rogers, D. C. Erbach

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A critical objective of belowground research is to collect and process representative soil samples. Mechanical devices have been developed to quickly take soil cores in the field; however, techniques to rapidly process large-diameter soil cores are lacking. Our objective was to design and construct a soil extraction–cutting system that could effectively reduce processing time. Soil cores were extracted from large diameter steel core tubes using a custom hydraulic cylinder device that vertically pushes the soil core to a desired depth increment before cutting in a horizontal direction with another hydraulically driven device. As many as eight large cores per hour …

Growth And Feed Efficiency Of Juvenile Channel Catfish Reared At Different Water Temperatures And Fed Diets Containing Various Levels Of Fish Meal, Menghe H. Li, Edwin H. Robinson, Brian C. Peterson, Terry D. Bates

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Channel catfish Ictalurus punctatus do not feed well at low temperatures. It is generally thought that a diet containing fish meal enhances feed palatability at low temperatures since fish meal is highly palatable to fish. There is a lack of information on the effects of fish meal levels on the growth performance of channel catfish reared at low temperatures. Therefore, a study was conducted in a recirculating system to examine the effects of fish meal levels on the feed consumption, weight gain, and feed efficiency of juvenile channel catfish reared at various temperatures. Fish with an initial weight of 9.6 …

Nationally Coordinated Evaluation Of Soil Nitrogen Mineralization Rate Using A Standardized Aerobic Incubation Protocol, T. S. Griffin, C. W. Honeycutt, S. L. Albrecht, K. R. Sistani, H. A. Torbert, B. J. Wienhold, B. L. Woodbury, R. K. Hubbard, J. M. Powell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aerobic incubation methods have been widely used to assess soil nitrogen (N) mineralization, but standardized protocols are lacking. A single silt loam soil (Catlin silt loam; fine-silty, mixed, superactive, mesic, Oxyaquic Arguidoll) was subjected to aerobic incubation at six USDA-ARS locations using a standardized protocol. Incubations were conducted at multiple temperatures, which were combined based on degree days (DD). Soil water was maintained at 60% waterfilled pore space (WFPS; constant) or allowed to fluctuate between 60 and 30% WFPS (cycle). Soil subsamples were removed periodically and extracted in 2 M potassium chloride (KCl); nitrate (NO₃) and ammonium (NH …

Areawide Suppression Of Fire Ants, M. D. Aubuchon, R. K. Vander Meer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The imported fire ants, Solenopsis invicta and S. richteri, were inadvertently introduced into the USA in the early 1900s and currently inhabit over 129 million ha in Puerto Rico and 12 southern states, from Texas to Virginia (Callcott and Collins, 1996; USDA-APHIS map). Imported fire ants have also become established in isolated sites in California, Arizona, New Mexico and Maryland. Strict quarantine procedures have limited the spread of this pest (Lockley and Collins, 1990), but eventually populations will expand westward in increasing numbers in New Mexico, Arizona and California. They will also move upward along the Pacific coast, southward …

The Hawaii Fruit Fly Areawide Pest Management Programme, Roger I. Vargas, Ronald F. L. Mau, Eric B. Jang, Robert M. Faust, Lyle Wong

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fruit flies (Diptera: Tephritidae) are among the most economically important pests attacking soft fruits worldwide (White and Elson-Harris, 1992). Four invasive species Mediterranean fruit fly or medfly (Ceratitis capitata), melon fly (Bactrocera cucurbitae), oriental fruit fly (Bactrocera dorsalis) and the so-called Malaysian fruit fly or solanaceous fruit fly (Bactrocera latifrons) - have been devastating to Hawaiian agriculture for over 100 years by infesting more than 400 different host plants. These fruit flies:

• Jeopardize development of a diversified tropical fruit and vegetable industry.

• Require that commercial fruits undergo quarantine treatment prior …

Short Communication- An Update On The Diversity Of Wolbachia In Spalangia Spp. (Hymenoptera: Pteromalidae), K.D. Floate, P.C. Coghlin, D. B. Taylor

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Results from 13 additional host populations improves resolution on the diversity of Wolbachia bacteria in Spalangia spp. (Hymenoptera: Pteromalidae). These bacteria are of interest because they can profoundly affect their host’s reproduction. Manipulating Wolbachia infections may provide a method to improve the efficacy of biocontrol agents including Spalangia spp.

Parasitoid wasps (Pteromalidae: Hymenoptera) are of interest as biocontrol agents for pest flies (Diptera: Muscidae) of livestock confinements; e.g. dairies, cattle feedlots, poultry houses, swine barns. Many of these wasps are infected with Wolbachia bacteria that can profoundly affect their host’s reproduction. Wolbachia induce parthenogenesis in Muscidifurax uniraptor Kogan & Legner …

Volatile Compounds From The Integument Of White Leghorn Chickens (Gallus Gallus Domesticus L.): Candidate Attractants Of Ornithophilic Mosquito Species, Ulrich R. Bernier, Sandra A. Allan, Brian Quinn, Daniel Kline, Donald Barnard, Gary Clark

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Candidate kairomones of ornithophilic mosquito species are reported from GC/MS analysis of compounds from the skin on the back, the feet, and feathers from the back of White Leghorn chickens (Gallus gallus domesticus). Hexane and ether extracts of chicken feathers differ significantly in attractiveness of Culex spp. mosquitoes. The active (hexane) extracts contained an abundance of alcohols, ketones, and diones. The inactive (ether) extracts contained aldehydes, which also were in the hexane extracts. Analysis of hexane extracts from chicken feet, skin, and feathers demonstrated the qualitative similarity in the compounds collected with subtle differences observed in the quantitative …

Discovery Of Diurnal Resting Sites Of Phlebotomine Sand Flies In A Village In Southern Egypt, Jerome Hogsette, Hanafi A. Hanafi, Ulrich R. Bernier, Daniel L. Kline, Emad Y. Fawaz, Barry D. Furman, David F. Hoel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In an attempt to find diurnal resting sites of adult phlebotomine sand flies, potential phlebotomine adult habitats were aspirated in the village of Bahrif in Aswan, Egypt. During this survey, sand flies were aspirated from low (30–45 cm high) irregular piles of mud bricks found under high date palm canopies between the village and the Nile River. There were 5 ♂♂and 7 ♀♀ of Phlebotomus papatasi and 3♂♂ of Sergentomyia schwetzi. Six of the 7 aspirated females were engorged with blood. A total of 78 sand flies was captured on 3 glue boards placed overnight on the ground next …

Enteric Bacteria Of Field-Collected Colorado Potato Beetle Larvae Inhibit Growth Of The Entomopathogens Photorhabdus Temperata And Beauveria Bassiana, Michael B. Blackburn, Dawn E. Gundersen-Rindal, Donald C. Weber, Phyllis A. W. Martin, Robert R. Farrar Jr.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The nematode Heterorhabditis marelatus fails to reproduce in the Colorado potato beetle, Leptinotarsa decemlineata, possibly due to interference from the enteric bacteria of the beetle. Specifically, the enteric bacteria inhibit the growth of Photorhabdus temperata, the enteric symbiont of the nematode, in vitro. However, previous work was based on a laboratory culture of L. decemlineata, and we wished to determine if similar bacteria were present in the field. Therefore, we cultured the enteric bacteria of fourth-instar larvae collected from the field at two locations in Maryland and Virginia. Representatives of the genera Pantoea, Enterobacter, Pseudomonas …

The Genetic Diversity, Relationships, And Potential For Biological Control Of The Lobate Lac Scale, Paratachardina Pseudolobata Kondo & Gullan (Hemiptera: Coccoidea: Kerriidae), S. Schroer, R. W. Pemberton, L. G. Cook, T. Kondo, P. J. Gullan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The lobate lac scale Paratachardina pseudolobata Kondo & Gullan (Kerriidae) is a polyphagous pest of woody plants in Florida (U.S.A.) the Bahamas, Christmas Island (Australia) and it has been reported from Cuba. Its recent appearance as a pest in these places indicates that this scale is introduced; however, its native range is unknown. Until 2006, this pest species was identified mistakenly as Paratachardina lobata (Chamberlin) [now P. silvestri (Mahdihassan)], which is native to India and Sri Lanka. Quarantine laboratory acceptance trials with Indian P. silvestri parasitoids indicated a strong immune response from P. pseudolobata. Gregarious development of encyrtid wasps …

A Core Set Of Microsatellite Markers For Western Corn Rootworm (Coleoptera: Chrysomelidae) Population Genetics Studies, Kyung Seok Kim, Uwe Stolz, Nicholas J. Miller, Miller R. Waits, Thomas Guillermaud, Douglas V. Sumerford, Thomas W. Sappington

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Interest in the ecological and population genetics of the western corn rootworm, Diabrotica virgifera virgifera LeConte, has grown rapidly in the last few years in North America and Europe. This interest is a result of a number of converging issues related to the increasing difficulty in managing this pest and the need to characterize and understand gene ßow in the context of insect resistance management. One of the key components needed for successful population genetics studies is the availability of suitable molecular markers. Using a standard group of microsatellite markers enables researchers from different laboratories to directly compare and share …

Entomopathogenic Fungal Endophytes, Fernando E. Vega, Francisco Posada, M. Catherine Aime, Monica Pava-Ripoll, Francisco Infante, Stephen A. Rehner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fungal endophytes are quite common in nature and some of them have been shown to have adverse effects against insects, nematodes, and plant pathogens.
Our research program is aimed at using fungal endophytes-mediated plant defense as a novel biological control mechanism against the coffee berry borer, the most devastating pest of coffee throughout the world. A survey of fungal endophytes in coffee plants from Hawaii, Colombia, Mexico, and Puerto Rico has revealed the presence of various genera of fungal entomopathogens, including Acremonium, Beauveria, Cladosporium, Clonostachys, and Paecilomyces. Two of these, B. bassiana and Clonostachys rosea …