Digital Commons Network^™

Dehydration Of Pollock Skin Prior To Gelatin Production, Cindy K. Bower, Roberto J. Avena-Bustillos, Katie A. Hietala, Cristina Bilbao-Sainz, Carl W. Olsen, Tara H. Mchugh

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Alaska pollock (Theragra chalcogramma) is the U.S.A.’s largest commercial fishery, with an annual catch of over 1 million tons. During pollock processing, the skins are discarded ormade into fish meal, despite their value for gelatin production. The absence of gelatin-processing facilities in Alaska necessitates drying of the skins before transport to decrease the moisture content, but conventional hot-air drying is expensive. This study evaluated a less energy-intensive technology, the use of desiccants for reducing water weight in pollock skins prior to shipment. To ensure that the functional properties of gelatin obtained from dried pollock skins were not affected …

Performance Of Larval Florida Pompano Fed Nauplii Of The Calanoid Copepod Pseudodiaptomus Pelagicus, Eric J. Cassiano, Cortney L. Ohs, Charles R. Weirich, Nancy E. Breen, Andrew L. Rhyne

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Florida pompano Trachinotus carolinus is a highly prized marine fish species, the larviculture of which currently includes the feeding of live rotifers and nauplii of brine shrimp Artemia spp. However, no previous studies have evaluated the feeding of copepod nauplii. In this study, the growth and survival of Florida pompano larvae fed nauplii of the calanoid copepod Pseudodiaptomus pelagicus were compared with those of larvae fed the standard reference diet of enriched rotifers Brachionus plicatilis. Experiments were conducted during the first 7–9 d posthatch (DPH), a period preceding the provision of Artemia nauplii. Treatments included feeding only copepod …

Whole-Body And Muscle Protein Metabolism Are Not Affected By Acute Deviations From Habitual Protein Intake In Older Men: The Hormonal Regulators Of Muscle And Metabolism In Aging (Horma) Study, Kevin E. Yarasheski, Carmen Castaneda-Sceppa, Jiaxiu He, Miwa Kawakubo, Shalender Bhasin, Ellen F. Binder, E. Todd Schroeder, Ronenn Roubenoff, Stan P. Azen, Fred R. Sattler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background:

Acute deviations in protein intake before the quantification of protein kinetics in older humans may explain the controversy over the effects of older age on muscle protein synthesis and proteolysis rates.

Objective:

We hypothesized that an acute decrease in protein intake from the habitual intake is associated with lower muscle protein synthesis and higher proteolysis rates, whereas an acute increase in protein intake from the habitual intake is associated with higher muscle protein synthesis and lower proteolysis rates.

Design:

In 112 community-dwelling healthy men aged 65–90 y, we quantified resting whole-body [1,2-¹³C₂]leucine kinetics, muscle mixed …

Fluid Fertilizer’S Role In Sustaining Soils Used For Bio-Energy Feedstock Production, John L. Kovar, Douglas L. Karlen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The use of corn (Zea mays L.) as a bio-energy feedstock has attracted the attention of many producers. Recently, the focus has shifted from grain-based to cellulose-based ethanol production. In addition to biological conversion of corn stover to ethanol, thermal conversion (pyrolysis) of stover is being explored. Regardless of post-harvest processing, the short- and long-term effects of both increasing grain yields and removing stover on soil nutrient cycling, physical properties, and biological activity must be understood to ensure that soil productivity and ecosystem services are maintained. Our objectives for 2010 were to evaluate: (i) the use of surface or …

Evaluation Of Waxy Grain Sorghum For Ethanol Production, Shuping Yan, Xiaorong Wu, Scott R. Bean, Jeffery F. Pedersen, Tesfaye Tesso, Yuanhong Chen, Donghai Wang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The objective of this research was to investigate the fermentation performance of waxy grain sorghum for ethanol production. Twenty-five waxy grain sorghum varieties were evaluated with a laboratory dry-grind procedure. Total starch and amylose contents were measured following colorimetric procedures. Total starch and amylose contents ranged from 65.4 to 76.3% and from 5.5 to 7.3%, respectively. Fermentation efficiencies were in the range of 86.0-92.2%, corresponding to ethanol yields of 2.61-3.03 gallons/bushel. The advantages of using waxy sorghums for ethanol production include easier gelatinization and low viscosity during liquefaction, higher starch and protein digestibility, higher free amino nitrogen (FAN) content, and …

Farmers' Markets In Kentucky: A Geospatial, Statistical, And Cultural Analysis, Elizabeth Ann Schmitz

Masters Theses & Specialist Projects

To assess what factors are driving the exponential growth of farmers’ markets in Kentucky, geospatial and statistical analysis of a database of 121 farmers’ markets was conducted. A statewide survey of market leaders and a case study of a single farmers’ market both identified reasons for growing support of farmers’ markets in Kentucky.

Market distribution, vendor levels, and gross sales were mapped against a backdrop of county urban classification, median household income, and education levels. Kruskal-Wallace analysis was used to identify if Kentucky’s rural, micropolitan, and metropolitan markets differ significantly in terms of their age, number of vendors, and market …

Enhancing Out-Of-Season Production Of Tomatoes And Lettuce Using High Tunnels, Britney L. Hunter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The growing season for vegetable crops is limited by freezing temperatures in arid high elevation climates such as northern Utah. Logan, Utah (41.73 N, 111.83 W, 1382 m elevation) has a short, variable growing season with an average frost-free period of 135 days. Extending the growing season provides growers with an opportunity to extend revenue into a normally unproductive period and benefit from out-of-season price premiums. High tunnels have been used to effectively extend the growing season for numerous crops by providing cold temperature protection. However, limited high tunnel research has been performed in arid high elevation regions that experience …

Susceptibility Of Isofamilies Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) To Cry1ac And Cry1fa Proteins Of Bacillus Thuringiensis, Carlos A. Blanco, Maribel Portilla, Juan Luis Jurat-Fuentes, Jaime F. Sanchez, Diego Viteri, Paulina Vega-Aquino, Antonio P. Teran-Vargas, Ausencio Azuara-Dominguez, Juan D. Lopez Jr., R. S. Arias, Yu-Cheng Zhu, David Lugo-Barreras, Ryan Jackson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The fall armyworm, Spodoptera frugiperda (J. E. Smith), is one of the most important insect pests on the American continent. Its control has relied primarily on multiple applications of insecticides that can amount to 1,000 g of active ingredient per hectare on some of approximately 30 crops the insect damages. The use of genetically engineered crops that express Bacillus thuringiensis (Bt) Berliner toxins, Bt-corn, Zea mays L.; and Bt-cotton, Gossypium hirsutum L.; are other ways to control this insect. However, fall armyworm is one of the Lepidoptera species least susceptible to Bt proteins, and a case of high tolerance to …

B. R. Wells Rice Research Studies 2009, R. J. Norman, K. A. K. Moldenhauer

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Growing Biserrula To Improve Grain And Livestock Production, Angelo Loi, Natalie Hogg, Clinton Revell, Diana Fedorenko

Bulletins 4000 -

Biserrula pelecinus is a new genus of pasture legume which was developed for commercial agriculture by a team of Perth-based legume pasture scientists in the early 1990s. Biserrula is an annual pasture legume that is found in natural grasslands of the Mediterranean Basin, frequently on acidic soils derived from granite. It was this feature that first caught the eye of researchers who were attempting to develop species that could tolerate the soil stresses of acidity and low moisture-holding capacity. These stresses are common in WA and when they occur together they are problematical for the survival of annual medics and …

Poisonous Rangeland Plants In San Luis Obispo County, Sara Litten, Amanda Ou

Animal Science

Poisonous Rangeland Plants in San Luis Obispo County is a comprehensive educational guide to rangeland plants that are toxic to domestic livestock. This guide begins with an exploration of how the biological systems are affected by the poisonous plant toxins. The biochemistry behind these toxins is included in the discussion. Next, reference material for fourteen plants that inhabit San Luis Obispo County is provided. This information includes specific toxins found in poisonous plants, affected animals, symptoms of poisoning, stages of growth, lethal dose, and distribution of the plant in California. This section of the guide is filled with helpful photos …

Good Agricultural Practices For Food Safety Of Fresh Produce, Laurie Hodges

Department of Agronomy and Horticulture: Faculty Publications

What are the guiding principles of food safety for fresh produce?

• Once contaminated, removing or killing pathogens on produce is very difficult.

• Preventing microbial contamination at all steps, from production to distribution, is strongly favored over treatments to eliminate contamination that may have occurred.

• Document the implementation of prevention programs, and educate workers at all levels of the agricultural and packing environments on food safety.

Key Signatures of a Credible Food Safety Program

The following is a condensed checklist of practices and conditions that reduce the potential for fresh produce to be exposed to pathogenic microbes. Review …

Intsormil Helps Improve Varieties Of Grains In Developing Countries, Lori Mcginnis

INTSORMIL Impacts and Bulletins

International efforts to aid food production in developing countries are based within the Institute of Agriculture and Natural Resources at the University of Nebraska–Lincoln.

The International Sorghum and Millet Collaborative Research Support Program (INTSORMIL CRSP) was established at UNL’s East Campus in 1979. Its goal is to improve the devel- opment of sorghum, millet, and other grains in Central America and Africa, said John Yohe, director since 1988.

Genome Sequencing And Analysis Of The Model Grass Brachypodium Distachyon, John Vogel, David Garvin, Todd C. Mockler, Jeremy Schmutz, Dan Rokhsar, Michael Bevan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Three subfamilies of grasses, the Ehrhartoideae, Panicoideae and Pooideae, provide the bulk of human nutrition and are poised to become major sources of renewable energy. Here we describe the genome sequence of the wild grass Brachypodium distachyon (Brachypodium), which is, to our knowledge, the first member of the Pooideae subfamily to be sequenced. Comparison of the Brachypodium, rice and sorghum genomes shows a precise history of genome evolution across a broad diversity of the grasses, and establishes a template for analysis of the large genomes of economically important pooid grasses such as wheat. The high-quality genome sequence, coupled …

Fluid Fertilizer’S Role In Sustaining Soils Used For Bio-Fuels Production, John L. Kovar, Douglas L. Karlen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The short- and long-term effects on soil nutrient cycling, physical properties, and biological activity of striving for higher grain yields and removing crop residues for bio-fuels production must be understood to provide more quantitative crop and soil management guidelines. This study focuses primarily on potassium (K) and sulfur (S) response by corn (Zea mays L.) grown for bio-energy feedstock production. Our objectives for 2009 were to evaluate (i) the performance of several S fertilizers, including liquid ammonium thiosulfate (12-0-0-26S), for corn grown in Iowa, and (ii) the use of surface or subsurface bands of N-P-K-S fluid fertilizers to optimize …

Procedures For Publishing In Non-Usda Media (Outside Publishing)

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This P&P is an update of a previous version dated March 10, 1998. This P&P outlines revised responsibilities of the ARS author, Research Leader (RL), and other ARS officials. The most salient features of the new P&P are that the manuscript approval authority and associated responsibilities are delegated to the RL. ARS Form 115 (ARS-115) is revised accordingly (see Exhibit 1). Among the essential RL responsibilities are to determine the need and method for manuscript peer review, approve the technical and interpretive content of the manuscript, notify upper management about manuscripts containing prominent issues, obtain proper clearance/approval from upper management …

U.S. Meat Animal Research Center Clay Center, Ne

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mission

Research Units

U.S. Meat Animal Research Center Animal Health Research Unit

U.S. Meat Animal Research Center Environmental Management Research Unit

U.S. Meat Animal Research Center Genetics & Breeding Research Unit

U.S. Meat Animal Research Center Meat Safety and Quality Research Unit

U.S. Meat Animal Research Center Nutrition Research Unit

U.S. Meat Animal Research Center Reproduction Research Unit

Effects Of A New Waste-Processing By-Product On Soil And Vegetation At Fort Campbell, Tennessee, R. R. Busby, D. L. Gebhart, H. A. Torbert, J. O. Dawson, G. A. Bollero, K. N. Potter, D. R. Curtin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A garbage-processing technology has been developed that sterilizes and separates inorganic and organic components of municipal solid waste. A study was initiated to evaluate the uncomposted organic by-product of this process as a soil amendment for establishing native prairie grasses on disturbed Army training lands. The waste was incorporated into a silt loam soil at Fort Campbell Military Reservation in the central United States. The waste material was applied at rates of 0, 4.5, 9, 18, and 36 Mg ha^-1 and seeded with native prairie grasses to assess its effects on vegetation for two growing seasons, with an additional …

‘Food For Life’: Looking Beyond The Horizon, Charles A. Francis, John W. Doran

Department of Agronomy and Horticulture: Faculty Publications

Our real challenge appears to be anticipating what the major challenges and constraints will be to food production and distribution a decade or more into the future. This could be called ‘Beyond the Horizon’ thinking. Today there is little disagreement over the massive depletion on a global scale of two essential inputs to agriculture: fossil fuels and fresh water. We also recognize that phosphorus is found in concentrated form in only a few deposits in nature, and that agriculture is rapidly using this limited resource and dispersing it through harvested products and soil loss from fields in forms that make …

Nutrient Accumulation And Photosynthesis In Glyphosate-Resistant Soybeans Is Reduced Under Glyphosate Use, Luiz Henrique Saes Zobiole, Rubem Silvério De Oliveira Jr., Robert John Kremer, Antonio Saraiva Muniz, Adilson De Oliveira Jr.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Global production of glyphosate-resistant (GR) soybean [Glycine max (L.) Merr.] continues to increase annually; however, there are no particular specific fertilizer recommendations for the transgenic varieties used in this system largely because reports of glyphosate effects on mineral nutrition of GR soybeans are lacking. Several metabolites or degradation products of glyphosate have been identified or postulated to cause undesirable effects on GR soybeans. In this work we used increasing glyphosate rates in different application on cv. ‘BRS 242 GR’ in order to evaluate photosynthetic parameters, macro- and micronutrient uptake and accumulation and shoot and root dry …

Cloning, Expression, And Immunogenicity Of Flavobacterium Columnare Heat Shock Protein Dnaj, Oscar Olivares-Fuster, Jeffery S. Terhune, Craig A. Shoemaker, Covadonga R. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Flavobacterium columnare heat shock protein (HSP) gene dnaJ* was isolated, cloned, expressed, and used as an antigen in a recombinant vaccine strategy for channel catfish Ictalurus punctatus. The F. columnare dnaJ* sequence was obtained from genomovars I and II and showed intraspecies variability. Recombinant protein was expressed and purified from Escherichia coli cultures and injected intraperitoneally (12 lg of purified DnaJ/fish) into fingerling channel catfish. In addition, induced (expressing the recombinant DnaJ) and uninduced (no recombinant protein being produced) E. coli cultures were also used to immunize fish. At 28 d postimmunization, antibody response was evaluated and the …

Production Characteristics And Body Composition Of Juvenile Cobia Fed Three Different Commercial Diets In Recirculating Aquaculture Systems, Charles R. Weirich, Paul S. Wills, Richard M. Baptiste, Marty A. Riche

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The effect of feeding three commercial diets on production characteristics and body composition of juvenile cobia Rachycentron canadum reared using recirculating aquaculture systems (RASs) was assessed in a 56-d growth trial. Juvenile cobia (mean weight ± SE, 29.2 ± 0.7 g) were stocked into three 8-m³ tanks in each of four RASs at an initial density of 1.2 kg/m³. After stocking, fish were fed one of three commercial diets: Hybrid Striped Bass diet (HSB; 44% protein, 12% lipid), Finfish Gold diet (GOLD; 42% protein, 16% lipid), or Marine Grower diet (MG; 50% protein, 15% lipid), all at …

Effects Of Prescribed Fire And Herbicide Application On Cattle Grazing And Herbage Production From Yellow Bluestem Pastures, S. A. Gunter, R. L. Gillen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Forage management practices influence the quantity and quality of forage available to grazing cattle. The objectives of this experiment were to determine the effects of prescribed fire or herbicides on the density of forbs, the production and nutritive value of the herbage, and the performance of beef steers grazing Yellow bluestem [Bothriochloa ischaemum (L.) Keng var. ischaemum (Hack.) Celarier and Harlan] pastures. The experiment was conducted in northwestern Oklahoma from 1998 through 2000, and the untreated pastures (control) were not burned or sprayed. The prescribed-fire treatment (PFT) occurred annually between March 27 and April 10. The herbicide treatment (HT) consisted …

Comparison Of Broiler Litter And Commercial Fertilizer At Equivalent N Rates On Soil Properties, Ardeshir Adeli, Haile Tewolde, Karamat Sistani, Dennis Rowe

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A 3-year study was conducted to determine the effects of broiler litter relative to inorganic fertilizer on soil nutrient content and quality in an upland Loring silt loam soil. Treatments included annual broiler litter rates of 0, 2.2, 4.5, 5.6, 6.7, 10.1, and 13.4 Mg ha⁻¹ y⁻¹ and commercial fertilizer rates of 34, 68, 90, 112, 134, and 168 kg nitrogen (N) ha⁻¹ y⁻¹. Broiler litter application linearly increased soil total carbon (C), microbial biomass C, extractable soil phosphorus (P), potassium (K), soil cation exchange capacity (CEC), and the stability of soil aggregate. At the …

Overseeding Unimproved Warm-Season Pasture With Cool- And Warm-Season Legumes To Enhance Forage Productivity, P. W. Bartholomew, R. D. Williams

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Overseeding forage legumes into existing warm-season pasture may help to reduce cool-season forage deficit on small and resource-limited small farms in the southern Great Plains of the United States. Unimproved warm-season grass pastures were overseeded with Korean lespedeza (Lespedeza stipulacea Maxim) were not overseeded with summer legume. These same plots were subsequently overseeded with hairy vetch (Vicia villosa Roth), crownvetch (Coronilla varia L.), black medic (Medicago lupulina L.) or ladino white clover (Trifolium repens L.) or, not overseeded with cool-season legume. Including lespedeza in a forage mixture increased total forage yield by an average of …

High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of …

Horticulture Of Ribes, K. E. Hummer, A. Dale

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The genus Ribes L., known as currants and gooseberries, contains more than 150 diverse species indigenous throughout the northern hemisphere and along the Rocky Mountain, Sierra Nevada and Sierra Madres in North America through mountain ranges of Central America to the Andes in South America. Beginning in the 1400s, four main crop types, black currants (Ribes, subgenus: Ribes, section: Botrycarpum), red and white currants (Ribes, subgenus: Ribes, section: Ribes) and gooseberries (Ribes, subgenus: Grossularia) were domesticated from European species. American and Eurasian species were selected and combined into …

Yeasts Associated With Nectarines And Their Potential For Biological Control Of Brown Rot, W. J. Janisiewicz, C. P. Kurtzman, J. S. Buyer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Resident fruit microflora has been the source of biocontrol agents for the control of postharvest decay of fruits and the active ingredient in commercialized biocontrol products. With the exception of grapes and apples, information on the resident microflora of other fruits is only fragmentary, but greater knowledge in this area can be very helpful in developing biocontrol strategies. We characterized the yeast microflora of nectarines (‘Croce del Sud’) from the early stages of fruit development until harvest. The fruit samples were collected from trees in an unmanaged orchard. The resident fruit microflora was separated from the occasionally deposited microorganisms by …

Current And Future Food Demand In Western Australia. Module 1 Scoping Study, Andrew Weinert, Robert R. Rouda

Farm Systems and Economics published reports

Maintaining and growing our food industries are priorities of the Western Australian Government and its Department of Agriculture and Food, Western Australia (DAFWA). Understanding our current food consumption levels, imports, exports and production limitations is critical to the formulation of effective agricultural and food polices.

The WA food industries are affected by several factors that include previous domestic policies and market instruments; general lack of scale; geographical isolation; access to suitable land and water; the availability and use of new technology, communication, processing and transport infrastructure that facilitate efficient marketing; sales and administration; social factors; international tariffs and other trade …

Wheat (Triticum Aestivum) Nam Proteins Regulate The Translocation Of Iron, Zinc, And Nitrogen Compounds From Vegetative Tissues To Grain, Brian M. Waters, Cristobal Uauy, Jorge Dubcovsky, Michael A. Grusak

Department of Agronomy and Horticulture: Faculty Publications

The NAM-B1 gene is a NAC transcription factor that affects grain nutrient concentrations in wheat (Triticum aestivum). An RNAi line with reduced expression of NAM genes has lower grain protein, iron (Fe), and zinc (Zn) concentrations. To determine whether decreased remobilization, lower plant uptake, or decreased partitioning to grain are responsible for this phenotype, mineral dynamics were quantified in wheat tissues throughout grain development. Control and RNAi wheat were grown in potting mix and hydroponics. Mineral (Ca, Cu, Fe, K, Mg, Mn, P, S, and Zn) and nitrogen (N) contents of organs were determined at regular intervals to …