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Intracellular Ph, The Proximate Signal For Cell Volume Changes That Are Mediated By The Actin Cytoskeleton, William Pasley
Intracellular Ph, The Proximate Signal For Cell Volume Changes That Are Mediated By The Actin Cytoskeleton, William Pasley
Theses and Dissertations
The relationship between initial intracellular pH (pHi) and associated cell volume change was investigated by simultaneous measurement of pHi and cell volume with fluorescence imaging in polarized fungiform taste receptor cells (TRCs) loaded with BCECF in vitro. Ammonium pulses caused a brief, reversible alkalinization in pHi and induced cell swelling. Sodium-acetate pulses reversible decreased TRC pHi and induced cell shrinkage. Removal weak acids and return to Control Ringer's solution (CR) causedTRC pHi and volume to overshoot baseline levels before fully recovering. Replacing CR with zero-sodium solution resulted in irreversible acidification of TRC pHi and induced cell swelling. Addition of sodium …