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Full-Text Articles in Psychology
Is The Area Postrema Resistant To The Anesthetic Effects Of Ms-222?, Austin T. Csiszar
Is The Area Postrema Resistant To The Anesthetic Effects Of Ms-222?, Austin T. Csiszar
Cal Poly Humboldt theses and projects
MS-222 is a common fish anesthetic that dampens neural activity by inhibiting neuronal voltage-gated sodium channels (Nav) required for action potentials. Previous experiments conducted by our lab’s primary investigator using fluorescence calcium imaging in zebrafish neurons resulted in the observation that neurons in the area postrema (AP), a circumventricular organ located in the dorsal hindbrain, displayed prominent fluorescence intensity during MS-222 induced sedation. I used the genetically encoded calcium indicator GCaMP6s to conduct fluorescence calcium imaging with the goal of recording neural activity in the larval zebrafish area postrema and optic tectum to compare the anesthetic effects of …
Effects Of Atomoxetine On Circadian Rhythms And Locomotor Activity, Rhiannon Ryder Crimmins
Effects Of Atomoxetine On Circadian Rhythms And Locomotor Activity, Rhiannon Ryder Crimmins
Cal Poly Humboldt theses and projects
Background: Attention deficit hyperactivity disorder (ADHD) is a common neurological disorder characterized by inattention, hyperactivity, and impulsive behavior. Many people with ADHD who are taking pharmacological treatments also report having sleep problems, and both children and adults tend to fall asleep later than neurotypical people. Since diagnosis and treatment of ADHD are on the rise, understanding how the disorder and common medications influence circadian rhythms is becoming more important. Zebrafish are a common animal model that have been shown to reliably represent features of many human disorders, including ADHD. Hypothesis: The specific hypotheses tested were: (H1) that a …
Identifying Neuron Clusters Controlling Movement Vigor, Jay A. Strabinick
Identifying Neuron Clusters Controlling Movement Vigor, Jay A. Strabinick
Cal Poly Humboldt theses and projects
Parkinson’s disease (PD) is a neurodegenerative disease that progressively decreases dopaminergic function. Lower amounts of dopamine may cause an innate motivational shift that decreases movement vigor when performing difficult tasks. In PD patients, bradykinesia, the slowing of movement, is characteristic of this decrease in vigor. The movement vigor hypothesis proposes that dopamine neurons modulate performance aspects of movement, like speed and persistence. This paper proposes a series experiments utilizing neuron recording techniques in zebrafish that would test the movement vigor hypothesis by determining whether in fact there is a distinct group of dopamine neurons that modulate movement vigor. Core elements …
Investigating Differing Degrees Of Foxo3a Expression In Adult Neural Stem Cells Between Age Groups In Zebrafish, Francis G. Bacik
Investigating Differing Degrees Of Foxo3a Expression In Adult Neural Stem Cells Between Age Groups In Zebrafish, Francis G. Bacik
Cal Poly Humboldt theses and projects
One factor influencing organismal longevity is the activity of transcription factors of the “fork head domain” family, otherwise known as “Forkhead box” (Fox) proteins. Studies of the four isoforms of the “O” subclass of Fox proteins found in human genes have revealed a direct relationship between FoxO3a-dependent gene expression and the conservation of neural stem cell (NSC) in the adult brain, specifically in the subgranular zone of the dentate gyrus in the hippocampus and the subventricular zone of the cortex. This transcription factor has also been shown to regulate apoptosis in nervous tissue during embryonic development in zebrafish. The current …
Test Of Bpa's Estrogenic Effects On Brain Aromatase Expression, Neural Activity, And Locomotive Behavior In Zebrafish Larvae, Ryan J. Mcauley
Test Of Bpa's Estrogenic Effects On Brain Aromatase Expression, Neural Activity, And Locomotive Behavior In Zebrafish Larvae, Ryan J. Mcauley
Cal Poly Humboldt theses and projects
Bisphenol A (BPA) is a well-known endocrine disrupting chemical that mimics the effects of estrogens. Aromatase B (Cyp19a1b) is a brain-specific enzyme that converts testosterone to estrogen and is highly upregulated in response to estrogen receptor activation localized to radial glial cells. During embryonic zebrafish development, there is a small window of time denoted by an increase in neurogenesis and estrogen receptor activity. Previous studies have demonstrated that a low dose BPA exposure (0.1µM) during this window causes hyperlocomotion in larval zebrafish, yet no further explanation for this behavior change has been described. The purpose of this study was to …
Are Caffeine’S Effects On Neuronal Calcium Levels Due To The Caffeine Metabolite, Paraxanthine?, Kendra Jean Hartsuyker
Are Caffeine’S Effects On Neuronal Calcium Levels Due To The Caffeine Metabolite, Paraxanthine?, Kendra Jean Hartsuyker
Cal Poly Humboldt theses and projects
Caffeine has diverse effects on neurons including, potentially, protection against Parkinson’s-related neurodegeneration. Caffeine may protect neurons from damage by limiting mitochondrial membrane permeability through a calcium-dependent mechanism. This study was a first step investigating calcium’s role in caffeine neuroprotection in vivo using zebrafish larvae. Elavl3:GCaMP6s zebrafish, which express a genetically encoded fluorescent calcium indicator protein in most CNS neurons, received caffeine (0, 50, 125, 250 µM, bath applied) in an ascending dose series during fluorescence calcium imaging of a central catecholaminergic nucleus (a proposed zebrafish homolog of the locus coeruleus, a structure affected in Parkinson’s disease). Parallel experiments tested effects …