Legal Studies Commons^™

Quality Management And Oversight Of Texas Forensic Science Service Providers, Sarah P. Chu

Dissertations, Theses, and Capstone Projects

Forensic science oversight in the U.S. largely relies upon voluntary third-party forensic laboratory accreditation programs. Without a national system of regulation and given the highly fragmented local systems of control, few forensic science service providers (FSSPs) are subject to regulatory oversight beyond their third-party accreditors. Texas is unique in its establishment of a robust statewide oversight system and a strong governmental culture of transparency, permitting this study of forensic quality management. This study consisted of two parts. The first part of this dissertation characterized and analyzed quality incident reports (QIRs) published by the Texas Department of Public Safety Crime Laboratory …

Examining The Relationship Between Legal Systems And Forensic Science: Current Issues And Proposed Solutions, Rowan Dunton

Themis: Research Journal of Justice Studies and Forensic Science

Forensics and legal systems are often seen working together, but the relationship between the two disciplines is thornier and more complex than it may appear on the surface. This paper will examine why courtrooms struggle to accommodate forensic fields, how the nature of science can impede its utilization in court, and where legal education may fall short in educating students about the forensic sciences. After the literature is reviewed, solutions will be proposed to address each area of concern. Where possible, these solutions expand on existing infrastructure and ideas to make them easier to incorporate. Since the greater-scope issue is …

The Crisis Of Femicides In Mexico, Alejandra Vela

Themis: Research Journal of Justice Studies and Forensic Science

Femicides are a growing issue in Mexico. These murders are intertwined with the incompetence of authorities, poverty, corruption, and organized crime. Authorities tend to blame victims by not taking missing reports seriously, nor do they conduct searches in these urgent situations. Authorities do not have DNA databases, space in morgues, and have little forensic science training. Ways of identifying remains involve odontology, bitemarks, and saliva. These provide a means of identifying through individual characteristics and DNA. Evidence is usually mishandled and not tested, or purposely lost to stop the investigation, thus, involving corruption. Criminal organizations, known as cartels, bribe authorities …

Evaluation Of Direct-To-Amplification Cell Lysis Techniques For Forensically Relevant Non-Sperm Cells, Rhea Arya

Master of Science in Forensic Science Directed Research Projects

While efforts have been made to reduce the pervasive backlog of sexual assault evidence collection kits, the actual laboratory process remains very time-consuming due to the requirement of a differential lysis step before DNA purification, as well as intricate mixture analysis towards the end of the DNA workflow. Previous work in the Dawson Green laboratory at VCU has developed alternative solutions for differential extractions with sexual assault samples using both an in-tube and microdevice assay. Prior work led to the identification of an alternative sperm lysis method – alkaline lysis using 1M NaOH. However, the current lysis method used for …

Optimization Of Microfluidic Device Methodology For Optical Trapping Epithelial Cells In A Mixture, Brittney N. Hackworth

Master of Science in Forensic Science Directed Research Projects

Evidence containing biological mixtures can make DNA interpretation more difficult due to ambiguous allele calls and artifacts that may be present within electropherograms. Separating out these cell mixtures can be advantageous to the analysts’ analysis methods for identifying STR profiles with higher accuracy. A potential cell separation method that could be implemented in the beginning of the forensic DNA workflow is optical trapping.

This research utilized a 5W ytterbium linearly polarized laser split into two optical traps, paired with a microfluidic device to isolate spermatozoa and vaginal epithelial cells from a 1:1 mixture of vaginal fluid and 1:20 diluted seminal …

Horizontal And Vertical Transmission Of Microbes Associated With Blow Flies Of Forensic Importance, Gabrielle Alisa Burton

Master of Science in Forensic Science Directed Research Projects

Entomological and microbiological evidence can be used in the estimation of postmortem intervals (PMI). This study observes the horizontal and vertical transmission of the microbial community associated with blow flies of forensic importance. Variable region four (V4) of the 16S ribosomal DNA (16S rDNA) was amplified and sequenced from various life stages of blow flies (n=72) using dual-index high throughput sequencing strategy on the MiSeq FGx platform. No significant difference in bacterial community structure was observed between blowfly adults with carrion access and blowfly adults with no carrion access. Dysgonomonas, unclassified Proteobacteria, Escherichia, Ignatzschia, Providencia, …

Comparison Of Cell Counts To Dna Yield In Mock Biological Evidence Samples, Jacob Sewell

Master of Science in Forensic Science Directed Research Projects

The implementation of rapid DNA analysis systems is of interest to forensic DNA casework, as this technology exhibits a sample-to-profile time of approximately two hours with limited handling of samples. This decreases the likelihood of contamination, and can potentially reduce DNA backlogs; however, current rapid DNA analysis systems do not execute one crucial step of the forensic DNA analysis workflow: human DNA quantitation. This step is necessary for DNA profiles to be compliant with the FBI’s Quality Assurance Standard 9.4, allowing DNA profiles to be uploaded and searched within the CODIS database. Coupling traditional qPCR methods with rapid DNA analysis …

Development And Validation Of Microrna Markers For Forensic Body Fluid Identification Using Rt-Qpcr, Jane Goble

Master of Science in Forensic Science Directed Research Projects

Body fluid identification is one of the first and most fundamental steps in the forensic DNA workflow. It allows analysts to determine where a sample might exist on an item of evidence, as well as the type of sample that they are dealing with. However, current serological methods have been documented to produce false or inaccurate results in some cases, and may consume large amounts of sample during processing. Molecular methods, such as the use of microRNA and microbial profiles have been introduced as a means to increase specificity of testing, and have even been combined into a singular assay …

Dna Transfer And Persistence On Non-Porous Surfaces Submerged In Spring Water [Preliminary Study], Morgan L.. Korzik, Josep De Alcaraz-Fossoul, Michael S. Adamowicz, David San Pietro

Forensic Science: Faculty and Staff Publications

Submerged items are often thought to lack evidentiary value. However, previous studies have shown the ability to recover DNA from submerged porous items for upwards of six weeks. The crevices or interweaving fibers in porous items are thought to protect DNA from being washed away. It is hypothesized that, because non-porous surfaces do not have the same traits that might aid in DNA retention, then DNA quantities and the number of donor alleles recovered would decrease over longer submersion periods. Additionally, it is hypothesized that DNA quantity and the number of alleles would be negatively affected by flow conditions. Neat …

Assessment Of The Acidified Hydrogen Peroxide Method On Uncontrolled Fired Cartridge Case Samples, Kylie S. Richardson

Master of Science in Forensic Science Directed Research Projects

The use of fingerprints to identify individuals in forensic cases has been useful for many years due to the persistence and individuality of the prints. In addition, the presence of firearms in forensic casework is steadily increasing over the years. This study aims to expand upon prior research in the development and recovery of latent prints on fired, brass ammunition using an acidified hydrogen peroxide method. Although latent prints are left on the cartridge casing of ammunition during the handling and loading of ammunition into the firearm, it is hypothesized that the pressure within the barrel of the firearm during …