Legal Studies Commons^™

Extraction Of Challenging Forensic Samples Using The Microgem Dna Extraction Kit, Falyn R. Vega

Student Theses

In forensic science, DNA extraction can be a tedious and resource-intensive process. Extraction with Proteinase K is an industry standard but has its drawbacks, such as requiring multiple ionic detergents and washing steps. MicroGEM has developed a new enzyme called forensicGEM that is temperature-dependent and compatible with mesophilic enzymes, offering complete DNA extraction in about 20 minutes in a single tube, limiting contamination, loss of sample, and working time. ForensicGEM can extract DNA from highly degraded samples, potentially leading to more complete STR profiles. Highly degraded tissue and bone samples were collected and extracted with the forensicGEM …

Developing A Maceration Method For Isolation Of Dna From Cancellous Bones, Bryan Bernal

Student Theses

Prior to isolating DNA, it is necessary to remove any biological materials that may be present on commingled remains. Manual maceration of the outer surface is time-consuming. This study will address this issue by developing a simple method for processing bone samples prior to DNA isolation. A liquid-based technique was applied to macerate bone fragments by incubating bone fragments in an enzyme solution, removing potential contaminants, followed by a bone tissue disruption. Swine (Sus scrofa) bones were used to simulate human bones. Microscopic analysis suggested this method was effective for surface material removal. The methods effect on the …

Semi-Quantitative Detection Of Signature Peptides In Body Fluids By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Tebah N. Browne

Student Theses

This study covers a modified semi-quantitative approach for liquid chromatography mass spectrometry (LC-MS/MS) signature peptide detection for body fluid identification. Peptide concentrations were measured based on synthetic peptide standards. Samples were processed with varying trypsin digestion and purification protocols, including a three-hour trypsin digestion and Microcon membrane filtration. The Microcon filtration method generates polymerase chain reaction (PCR) compatible DNA and peptide fractions that can be analyzed without any further purification. Preliminary validation tests covered stains on different substrates, semen/ saliva mixtures, minimum sample volume, and repeatability. All signature peptides in the multiplex were present at different concentrations and varied amongst …

Compound-Specific Isotope Analysis Of Amino Acids In Biological Tissues: Applications In Forensic Entomology, Food Authentication And Soft-Biometrics In Humans, Mayara Patricia Viana De Matos

Graduate Theses, Dissertations, and Problem Reports

In this work we demonstrate the power of compound-specific isotope analysis (CSIA) to analyze proteinaceous biological materials in three distinct forensic applications, including: 1) linking necrophagous blow flies in different life stages to their primary carrion diet; 2) identifying the harvesting area of oysters for food authentication purposes; and 3) the ability to predict biometric traits about humans from their hair.

In the first application, we measured the amino-acid-level fractionation that occurs at each major life stage of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) blow flies. Adult blow flies oviposited on raw pork muscle, beef muscle, or chicken liver. Larvae, pupae …

Furthering Understanding Of Forensic Units: A Detailed Examination Of Knoxville Police Department's Crime Scene Unit., Cassandra Christina Rausch

Electronic Theses and Dissertations

Throughout the past three decades, the criminal justice system has decidedly employed new technologies for the purposes of establishing guilt or innocence. Forensic science, with its synthesis of scientific methodology and investigative considerations of law enforcement, ushered in the development of DNA profiling and forever changed the process of crime scene investigation.This shift in focus also led to a shift in the individuals involved in investigation, producing the widespread formation of stand-alone crime scene units. Utilizing both civilian and sworn employees of a law enforcement agency, these units became responsible for the documentation, collection, and preservation of evidence that would …

Exploring Various Techniques To Process And Identify Latent Friction Ridge Details From Fingers On Semi-Porous Surfaces, Emily Campo

Honors Theses

Numerous terrorist groups in the Middle East have been recently making detonators for IEDs (Improvised Explosive Devices) using items as basic as flip flops. Because of the semi-porous nature of flip flops, military forensic response teams have been unable to find an effective method through which they can identify, process, and lift latent fingerprints from the surface of a flip flop. The present study aims to use three fingerprint principles to formulate a reliable method that can be used for such purposes. This study specifically investigated the potential use of powders and cyanoacrylate fuming as processing techniques. The case study …

Forensic Markers Of Lampricide Toxicity In Oncorhynchsus Mykiss, Chris White

Theses and Dissertations (Comprehensive)

The lampricide 3-trifluoromethyl-4-nitrophenol (TFM) has been used for decades to control invasive sea lamprey (Petromyzon marinus) populations in the Great Lakes, normally with little harm to other fish populations. However, due to the nature of lampricide treatments and the chemical properties of TFM, adverse effects to non-target fishes and non-target mortality occasionally occur. However, investigations of non-target mortality can be complex, particularly if fish deaths are not noticed immediately, due to decomposition of the carcasses. The objectives of this thesis were to expose rainbow trout (Oncorhynchsus mykiss) to TFM in order to: (i) identify forensic markers …

"It's Not About You": Exploring The Liminal Experiences Of Graduate Forensic Coaches, Christopher Paul Outzen

All Graduate Theses, Dissertations, and Other Capstone Projects

The following document is a capstone thesis project focusing on the unique experiences of graduate forensic coaches through lens of liminality, a performance theory used to describe a sense of being between social identities when going through a rite of passage. The author contends that this liminal experience has unique characteristics which are important to consider in the context of identity and forensic culture. In order to gather data, the author utilized qualitative, semi-structured interviews with current graduate forensic coaches. The resulting interviews were interpreted using a process of open coding to determine key themes of the experience. The author …

Development Of Micro Volume Dna And Rna Profiling Assays To Identify The Donor And Tissue Source Of Origin Of Trace Forensic Biological Evidence, Brittany Morgan

Electronic Theses and Dissertations

In forensic casework analysis it is necessary to obtain genetic profiles from increasingly smaller amounts of biological material left behind by perpetrators of crime. The ability to obtain profiles from trace biological evidence is demonstrated with so-called ‘touch DNA evidence’ which is perceived to be the result of DNA obtained from shed skin cells transferred from donor to an object or person during physical contact. However, the current method of recovery of trace DNA involves cotton swabs or adhesive tape to sample an area of interest. This "blindswabbing" approach may result in the recovery of biological material from different individuals …

A Forensic Identification Utility To Create Facial Approximations Using Cone-Beam Computed Tomography Of 100 Hispanic Females: A Pilot Study, Behzad Nejat

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction:Estimation of facial soft tissue appearance from human skeletal remains is often necessary in forensic identification. This process has been referred to as facial reconstruction or facial approximation and is a branch of forensic facial anthropology. Original methods for facial approximation originated in nineteenth century Europe and consisted of artists shaping clay over skull models using average soft tissue depths measured in cadavers. The last two decades have introduced numerous computerized techniques that have digitized this process while attempting to accurately and objectively define the relationship between a skull and its overlying soft tissue. This pilot study describes a method …

Real World Computer Forensics, Jessica Riccio

Liberal Arts and Engineering Studies

No abstract provided.

Older, Wiser, Novice: An Autoethnographic Study Of Nontraditional Students' Participation In Collegiate Forensics, Laura Kathleen Pelletier

All Graduate Theses, Dissertations, and Other Capstone Projects

There is a growing trend in nontraditional college student enrollments in the United States. Older, nontraditional students are currently the majority on many college campuses. Due to the constraints on nontraditional students' time, they are often unable to spend as much time on campus as traditional students and are unable to fully partake in campus life and socialization. Cocurricular activities, such as collegiate forensics, can be time consuming activities which for nontraditional students, especially those who have children, may seem like an impossible fit for their already busy schedules. Because college demographics continue to change and there are a growing …

Forensics And The Basic Communication Course: A New Path To Satisfying Learning Outcomes, Benjamin Walker

All Graduate Theses, Dissertations, and Other Capstone Projects

Forensic scholars have long written about the educational benefits of forensics, but very few have attempted to tie the activity to learning objectives from the curriculum. This thesis seeks to determine if collegiate forensics can offer the same learning opportunities as one of the most common and fundamental communication classes in the discipline: the basic communication course. This research uses experiential learning as a pedagogical framework for forensics in attempting to answer if forensics can offer the same learning opportunities of the basic communication course, and if so, how the activity does this and what the students actually learn. Likert …

Defining Success: Examining What It Means To Be Good In Forensics, David J. Brennan

All Graduate Theses, Dissertations, and Other Capstone Projects

For decades college students have been competing in forensic activity (forensics), spending countless hours researching, writing, and performing speeches. Forensic scholars have never created an overarching definition of what it means to be successful in forensics. A survey was created and sent out on the individual events listserv, asking competitors and coaches to define success. Analysis reveals both competitors and coaches believe success in forensics is a combination of competitive achievement, building communication skills, and personal growth.