Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Legal Studies
Evaluation Of Direct-To-Amplification Cell Lysis Techniques For Forensically Relevant Non-Sperm Cells, Rhea Arya
Master of Science in Forensic Science Directed Research Projects
While efforts have been made to reduce the pervasive backlog of sexual assault evidence collection kits, the actual laboratory process remains very time-consuming due to the requirement of a differential lysis step before DNA purification, as well as intricate mixture analysis towards the end of the DNA workflow. Previous work in the Dawson Green laboratory at VCU has developed alternative solutions for differential extractions with sexual assault samples using both an in-tube and microdevice assay. Prior work led to the identification of an alternative sperm lysis method – alkaline lysis using 1M NaOH. However, the current lysis method used for …