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Articles 1 - 30 of 83

Full-Text Articles in Social and Behavioral Sciences

Mental Help-Seeking: Barriers Among African Americans: The Role Of Technology In Addressing Those Barriers, Charneka Edwards May 2024

Mental Help-Seeking: Barriers Among African Americans: The Role Of Technology In Addressing Those Barriers, Charneka Edwards

Electronic Theses, Projects, and Dissertations

The topic of mental health among the African American community is not often examined on its own, despite nuanced differences contributing to their reluctance to seek mental health services. The study is to examine, through data collection, the various barriers that have contributed to the prevention and reluctance of African Americans regarding mental health help-seeking, and how technology can circumvent those barriers. African Americans make up 12% of the US population and, of the 47 million who identified as African American, only 8.6% sought traditional mental health services, while 56% reported underutilizing mental health services in the United States due …


Neural Correlates Of Audiovisual Integration, Jonathan Conrady Oct 2023

Neural Correlates Of Audiovisual Integration, Jonathan Conrady

Theses and Dissertations

Successfully integrating multiple streams of information is paired with a variety of behavioral enhancements. Multisensory research over the past few decades has demonstrated the criticality of spatial proximity and stimulus effectiveness. Here, we focused on the temporal aspects and underlying neural activity of frequency-varying, audiovisual stimuli using scalp recorded electroencephalography (EEG). Our set of stimuli was particularly chosen because humans are most sensitive to the auditory sound and visual spatial frequencies that make up speech. Recent work has shown that audiovisual integration effects of low-level, simple stimuli are largest when both modalities fall within, compared to outside of, their respective …


Structural Analysis And Interpretation Of Deformation Along The Keweenaw Fault System From Lake Linden To Mohawk, Michigan, Nolan G. Gamet Jan 2023

Structural Analysis And Interpretation Of Deformation Along The Keweenaw Fault System From Lake Linden To Mohawk, Michigan, Nolan G. Gamet

Dissertations, Master's Theses and Master's Reports

The Keweenaw fault is likely the most significant and most studied fault associated with the Midcontinent Rift System. The fault roughly bisects the Keweenaw Peninsula and places Portage Lake Volcanics (~1.1 Ga) over much younger Jacobsville Sandstone (~1.0 Ga). Published bedrock geology maps with cross sections from the 1950s show the fault as a single continuous trace that is locally associated with smaller cross faults and splays. The accompanying cross-sections show hanging-wall volcanic strata having a well-defined, listric geometry with dip decreasing away from the fault to the northwest.

This M.S. thesis presents a structural analysis and interpretation of the …


What Are The Barriers Experienced By Self-Represented Litigants In Civil Court?, Darwin Fitzgerald Rice Jan 2022

What Are The Barriers Experienced By Self-Represented Litigants In Civil Court?, Darwin Fitzgerald Rice

Walden Dissertations and Doctoral Studies

The Judiciary Act of 1789 permits parties to plead and manage their cases personally or by a defense counsel. The legal provision laid the foundation for self-representation guidelines adopted by the courts. Despite self-representation becoming widely accepted in the legal system, there is limited understanding of the barriers faced by litigants. John Rawls’ theory of Justice written in 1971 guided an in-depth analysis of these experiences. The theory states that with the subject of justice things are just or unjust. With the need to answer the research questions that sought to explore the lived experiences of self-represented litigants, attorneys, and …


An Evaluation Of Knot Placement Strategies For Spline Regression, William Klein Jan 2021

An Evaluation Of Knot Placement Strategies For Spline Regression, William Klein

CMC Senior Theses

Regression splines have an established value for producing quality fit at a relatively low-degree polynomial. This paper explores the implications of adopting new methods for knot selection in tandem with established methodology from the current literature. Structural features of generated datasets, as well as residuals collected from sequential iterative models are used to augment the equidistant knot selection process. From analyzing a simulated dataset and an application onto the Racial Animus dataset, I find that a B-spline basis paired with equally-spaced knots remains the best choice when data are evenly distributed, even when structural features of a dataset are known …


Spiritual Knight Mission In Novel Asmara Djibrat Ludira, Fahmi Iqbal Ibrahim,, Darmoko Darmoko Jul 2020

Spiritual Knight Mission In Novel Asmara Djibrat Ludira, Fahmi Iqbal Ibrahim,, Darmoko Darmoko

International Review of Humanities Studies

One result of culture is literary work, but broadly speaking literature is the work of individuals, only the object delivered will not be separated from the culture and social life of the community. The close relationship between literature and culture can produce literary works that have a function as cultural preservation. A complex culture can be reflected in a literary work. If traced carefully, it can be known that some authors have included a tradition and culture of an area in their literary work. One type of imaginary story is a novel. A novel is a term for a long, …


The Scopes Of Discourse, H. S. Matnazarova Mar 2019

The Scopes Of Discourse, H. S. Matnazarova

Central Asian Problems of Modern Science and Education

This article deals with the classification problems of discourse in English and considers different hypothesis on the matter. Discourse is analyzed as an important aspect of cognition and cognitive linguistics. Its analysis takes into consideration communicative conditions and functions.


The Scopes Of Discourse, H. S. Matnazarova Jan 2019

The Scopes Of Discourse, H. S. Matnazarova

Central Asian Problems of Modern Science and Education

This article deals with the classification problems of discourse in English and considers different hypothesis on the matter. Discourse is analyzed as an important aspect of cognition and cognitive linguistics. Its analysis takes into consideration communicative conditions and functions.


Perceptions Of Vulnerability To Flooding, Hurricanes, And Climate Change On Grand Isle, Louisiana’S Only Inhabited Barrier Island, Lauren Miller Jan 2019

Perceptions Of Vulnerability To Flooding, Hurricanes, And Climate Change On Grand Isle, Louisiana’S Only Inhabited Barrier Island, Lauren Miller

Graduate Student Theses, Dissertations, & Professional Papers

This study used in-depth interviews of permanent residents on Grand Isle, Louisiana, a remote barrier island, to better understand their perceptions of structural flood measures, non-structural responses to flooding and hurricanes, and perceptions of vulnerability to flooding, hurricanes, and climate change on a remote barrier island-Grand Isle, Louisiana. Residents' perceptions regarding the various structural measures implemented by the federal, state, and local government appeared mixed. Non-structural responses to flooding risks implemented at the household, community, state, and federal level continue to strengthen resiliency on Grand Isle. According to interviewees, aspects of environmental, rural, and economic vulnerability on Grand Isle impact …


Structural Principles Analysis Of Host-Pathogen Protein-Protein Interactions: A Structural Bioinformatics Survey, Huaming Chen, William Guo, Jun Shen, Lei Wang, Jiangning Song Jan 2018

Structural Principles Analysis Of Host-Pathogen Protein-Protein Interactions: A Structural Bioinformatics Survey, Huaming Chen, William Guo, Jun Shen, Lei Wang, Jiangning Song

Faculty of Engineering and Information Sciences - Papers: Part B

No abstract provided.


Impact Of Structural Design Solutions On The Energy And Thermal Performance Of An Australian Office Building, Mehdi Robati, Georgios Kokogiannakis, Timothy J. Mccarthy Jan 2017

Impact Of Structural Design Solutions On The Energy And Thermal Performance Of An Australian Office Building, Mehdi Robati, Georgios Kokogiannakis, Timothy J. Mccarthy

Faculty of Engineering and Information Sciences - Papers: Part B

Concrete is a heavyweight construction material whose high thermal mass could increase the thermal storage capacity of a building envelope and in turn affect indoor thermal comfort. Selecting an appropriate method for concrete construction and form could also affect the total energy performance and thermal comfort of a building, a fact that is often overlooked by structural engineers. This study presents the results of energy simulations of the potential impact that concrete construction forms, in particular two slab types, and structural materials have on the energy consumption of archetypal commercial office buildings in five major Australia cities (Sydney, Melbourne, Canberra, …


Towards Elucidating The Structural Principles Of Host-Pathogen Protein-Protein Interaction Networks: A Bioinformatics Survey, Huaming Chen, Jiangning Song, Geng Sun, Jun Shen, Lei Wang Jan 2017

Towards Elucidating The Structural Principles Of Host-Pathogen Protein-Protein Interaction Networks: A Bioinformatics Survey, Huaming Chen, Jiangning Song, Geng Sun, Jun Shen, Lei Wang

Faculty of Engineering and Information Sciences - Papers: Part B

The ultimate goal of systems biology research area is to accurately predict the behavior of biological systems through the construction of computational models, using the related molecular-level data as the input, especially when the structural information of such biological system is available. Combining the three-dimensional (3D) structural information of the cohort of macromolecules underpinning the biological system, the researchers are poised with an unprecedented opportunity to gain a full understanding on how the molecules interact with each other, particularly for an interaction network, e.g. protein-protein interaction networks. Specifically, there are currently a limited number of studies focused on the reconstruction …


Biaxial Creep Test Study On The Influence Of Structural Anisotropy On Rheological Behaviour Of Hard Rock, Chuangzhou Wu, Qingsheng Chen, Sudip Basack, Riqing Xu, Zhenming Shi Jan 2016

Biaxial Creep Test Study On The Influence Of Structural Anisotropy On Rheological Behaviour Of Hard Rock, Chuangzhou Wu, Qingsheng Chen, Sudip Basack, Riqing Xu, Zhenming Shi

Faculty of Engineering and Information Sciences - Papers: Part A

Rheological characteristics are one of most important properties needed to be considered for the designing and construction for the long term stability and serviceability of underground structures in the rock mass. Up to date, although extensive studies on the rheological properties of rocks are available in the literature, most of existing studies reported the strain-time data for the axial deformation through compression rheological method and did not mention the lateral deformation, and mainly focused on the soft rocks at shallow depth. Thus, very limited attention has been paid to the rheological properties of deep and hard rock, neglecting the effects …


Structural Behaviours Of Railway Prestressed Concrete Sleepers (Crossties) With Hole And Web Openings, Sakdirat Kaewunruen, Erosha Kahawatta Gamage, Alex M. Remennikov Jan 2016

Structural Behaviours Of Railway Prestressed Concrete Sleepers (Crossties) With Hole And Web Openings, Sakdirat Kaewunruen, Erosha Kahawatta Gamage, Alex M. Remennikov

Faculty of Engineering and Information Sciences - Papers: Part A

As the crosstie beam in railway track systems, the prestressed concrete sleepers (or railroad ties) are principally designed in order to carry wheel loads from the rails to the ground. Their design takes into account static and dynamic loading conditions. It is evident that prestressed concrete has played a significant role as to maintain the high endurance of the sleepers under low to moderate repeated impact loads. In spite of the most common use of the prestressed concrete sleepers in railway tracks, there have always been considerable demands from rail and track engineers to improve serviceability and functionality of concrete …


Online Coordinated Voltage Control In Distribution Systems Subjected To Structural Changes And Dg Availability, Dothinka Ranamuka Rallage, Ashish P. Agalgaonkar, Kashem M. Muttaqi Jan 2016

Online Coordinated Voltage Control In Distribution Systems Subjected To Structural Changes And Dg Availability, Dothinka Ranamuka Rallage, Ashish P. Agalgaonkar, Kashem M. Muttaqi

Faculty of Engineering and Information Sciences - Papers: Part A

The responses of multiple DG units and voltage regulating devices such as tap changers and capacitor banks for correcting the voltage may lead to operational conflicts and oscillatory transients, where distribution systems are subjected to network reconfiguration and changes in availability of the DG units. Therefore, coordinated voltage control is required to minimize control interactions while accounting for the impact of structural changes associated with the network. This paper proposes a strategy for coordinating the operation of multiple voltage regulating devices and DG units in medium voltage (MV) distribution systems, under structural changes and DG availability, for effective voltage control. …


Coupled Grain Boundary Motion In Aluminium: The Effect Of Structural Multiplicity, Kuiyu Cheng, Liang Zhang, Cheng Lu, Anh Kiet Tieu Jan 2016

Coupled Grain Boundary Motion In Aluminium: The Effect Of Structural Multiplicity, Kuiyu Cheng, Liang Zhang, Cheng Lu, Anh Kiet Tieu

Faculty of Engineering and Information Sciences - Papers: Part A

The shear-induced coupled grain boundary motion plays an important role in the deformation of nanocrystalline (NC) materials. It has been known that the atomic structure of the grain boundary (GB) is not necessarily unique for a given set of misorientation and inclination of the boundary plane. However, the effect of the structural multiplicity of the GB on its coupled motion has not been reported. In the present study we investigated the structural multiplicity of the symmetric tilt Σ 5(310) boundary in aluminium and its influence on the GB behaviour at a temperature range of 300 K-600 K using molecular dynamic …


Combined Bearing And Shear-Out Capacity Of Structural Steel Bolted Connections, Lip H. Teh, Mehmet Eren Uz Jan 2016

Combined Bearing And Shear-Out Capacity Of Structural Steel Bolted Connections, Lip H. Teh, Mehmet Eren Uz

Faculty of Engineering and Information Sciences - Papers: Part A

This study is concerned with the strength limit state of serial bolted connections in structural steel plates. It points out that the ultimate load capacity of a serial bolted connection failing in combined bearing and shear-out cannot be computed as the simple sum of the respective ultimate bearing and ultimate shear-out capacities, which is implicitly permitted in design specifications worldwide. Based on the laboratory test results of 10 hot-rolled steel plate specimens composed of three different grades with nominal thicknesses ranging from 5 to 8 mm, the paper first establishes the ultimate bearing coefficient of a 20-mm bolted connection in …


Structural, Functional, And Behavioral Alterations To The Dopamine System In The Female Hiv-1 Transgenic Rat, Robert Francis Roscoe Jr. Jan 2016

Structural, Functional, And Behavioral Alterations To The Dopamine System In The Female Hiv-1 Transgenic Rat, Robert Francis Roscoe Jr.

Theses and Dissertations

HIV-associated cognitive disorders continue to affect approximately 25 million individuals worldwide, and its prevalence is expected to increase as the lifespan of HIV-1 infected individuals continues to improve. The HIV-1 transgenic rat expresses 7 of the 9 genes that encompass the virus, and is an appropriate model for studying chronic HIV-1 infection at a level that is controlled through combined antiretroviral therapy. Psychostimulant abuse is known to exacerbate HAND symptomology, but the psychostimulant methylphenidate is a first line of treatment for HIV associated cognitive inhibition. Dopamine transport abnormalities in the HIV-1 Tg rat have been well characterized through behavioral testing; …


Structural Evolution Of Electrodes In The Ncr And Cgr Cathode-Containing Commercial Lithium-Ion Batteries Cycled Between 3.0 And 4.5 V: An Operando Neutron Powder-Diffraction Study, Wei Kong Pang, Moshiul Alam, Vanessa K. Peterson, Neeraj Sharma Jan 2015

Structural Evolution Of Electrodes In The Ncr And Cgr Cathode-Containing Commercial Lithium-Ion Batteries Cycled Between 3.0 And 4.5 V: An Operando Neutron Powder-Diffraction Study, Wei Kong Pang, Moshiul Alam, Vanessa K. Peterson, Neeraj Sharma

Faculty of Engineering and Information Sciences - Papers: Part A

The dissimilar lattice-evolution of the isostructural layered Li(Ni,Co,Al)O2 (NCR) and Li(Ni,Co,Mn)O2 (CGR) cathodes in commercial lithium-ion batteries during overcharging/discharging was examined using operando neutron powder-diffraction. The stacking axis (c parameter) of both cathodes expands on initial lithiation and contracts on further lithiation. Although both the initial increase and later decrease are smaller for the CGR cathode, the overall change between battery charged and discharged states of the c parameter is larger for the CGR (1.29%) than for the NCR cathode (0.33%). We find these differences are correlated to the transition metal to oxygen bond (as measured through …


Early Integration Of The Individual Student In Academic Activities: A Novel Classroom Concept For Graduate Education In Molecular Biophysics And Structural Biology, Sanford H. Leuba, Sean M. Carney, Elizabeth M. Dahlburg, Rebecca J. Eells, Harshad Ghodke, Naveena Yanamala, Grant Schauer, Judith Klein-Seetharaman Jan 2014

Early Integration Of The Individual Student In Academic Activities: A Novel Classroom Concept For Graduate Education In Molecular Biophysics And Structural Biology, Sanford H. Leuba, Sean M. Carney, Elizabeth M. Dahlburg, Rebecca J. Eells, Harshad Ghodke, Naveena Yanamala, Grant Schauer, Judith Klein-Seetharaman

Faculty of Science, Medicine and Health - Papers: part A

Background: A key challenge in interdisciplinary research is choosing the best approach from a large number of techniques derived from different disciplines and their interfaces.

Results: To address this challenge in the area of Biophysics and Structural Biology, we have designed a graduate level course to teach students insightful use of experimental biophysical approaches in relationship to addressing biological questions related to biomolecular interactions and dynamics. A weekly seminar and data and literature club are used to compliment the training in class. The course contains wet-laboratory experimental demonstration and real-data analysis as well as lectures, grant proposal preparation and assessment, …


Personal Best Goal And Self-Regulation As Predictors Of Mathematics Achievement: A Multilevel Structural Equation Model, Magdalena Mo Ching Mok, Michael Ying Wah Wong, Michael Ronald Su, Jim S. Tognolini, Gordon Stanley Jan 2014

Personal Best Goal And Self-Regulation As Predictors Of Mathematics Achievement: A Multilevel Structural Equation Model, Magdalena Mo Ching Mok, Michael Ying Wah Wong, Michael Ronald Su, Jim S. Tognolini, Gordon Stanley

Faculty of Social Sciences - Papers (Archive)

This study aimed to identify personal best goal and self-regulation as predictors of mathematics achievement for primary students. The sample comprised 3,821 (2,021 males and 1,800 females) students between Grades 3 to Grade 5 at 26 primary schools in Hong Kong. Students' personal best goals and self-regulation were used to predict their mathematics achievement six months later. Multilevel structural equation models were fitted to the data using the MPLUS software. Results showed that after controlling for student gender and grade level, students' personal best goal predicted their subsequent mathematics achievement. In contrast, self-regulation had no direct effect on students' mathematics …


Structural Equation Modelling To Assess Relationships Between Event-Related Potential Components, Heart Rate And Skin Conductance In The Context Of Emotional Stimuli, Susan Thomas, Peter Leeson, Craig Gonsalvez, Stuart Johnstone Jan 2014

Structural Equation Modelling To Assess Relationships Between Event-Related Potential Components, Heart Rate And Skin Conductance In The Context Of Emotional Stimuli, Susan Thomas, Peter Leeson, Craig Gonsalvez, Stuart Johnstone

Faculty of Social Sciences - Papers (Archive)

Abstract of a poster presented at the 17th World Congress of Psychophysiology (IOP2014) of the International Organization of Psychophysiology (IOP) Hiroshima, Japan, September 23rd to 27th, 2014.


Reliability-Based Conversion Of A Structural Design Code For Railway Prestressed Concrete Sleepers, A M. Remennikov, Martin Murray, Sakdirat Kaewunruen Oct 2013

Reliability-Based Conversion Of A Structural Design Code For Railway Prestressed Concrete Sleepers, A M. Remennikov, Martin Murray, Sakdirat Kaewunruen

Alex Remennikov

"Ballasted railway track is very suitable for heavy-rail networks because of its many superior advantages in design, construction, short- and long-term maintenance, sustainability, and life cycle cost. An important part of the railway track system, which distributes the wheel load to the formation, is the railway sleeper. Improved knowledge has raised concerns about design techniques for prestressed concrete (PC) sleepers. Most current design codes for these rely on allowable stresses and material strength reductions. However, premature cracking of PC sleepers has been found in railway tracks. The major cause of cracking is the infrequent but high-magnitude wheel loads produced by …


Structural Re-Assignment Of The Mono- And Bis-Addition Products From The Addition Reactions Of N-(Diphenylmethylene)Glycinate Esters To [60]Fullerene Under Bingel Conditions, Graham E Ball, Glenn Ashley Burley, Leila Chaker, William Hawkins, James Williams, Paul A. Keller, Stephen G. Pyne Sep 2013

Structural Re-Assignment Of The Mono- And Bis-Addition Products From The Addition Reactions Of N-(Diphenylmethylene)Glycinate Esters To [60]Fullerene Under Bingel Conditions, Graham E Ball, Glenn Ashley Burley, Leila Chaker, William Hawkins, James Williams, Paul A. Keller, Stephen G. Pyne

Paul Keller

The addition of N-(diphenylmethylene)glycinate esters (Ph2C=NCH2CO2R) to [60]fullerene under Bingel conditions gives [60]fullerenyldihydropyrroles and not methano[60]fullerenyl iminoesters [C60C(CO2R)(N=CPh2)] as previously reported. Unequivocal evidence for the structure of C60C(CO2Et)(N=CPh2) was provided by INADEQUATE NMR studies on 13C enriched material. New mechanistic details are proposed to account for the formation of [60]fullerenyldihydropyrroles and their reductive ring-opening reactions.


Project-Based Section 8 Housing Participants' Perception Of Cultural And Structural Factors To Explain Barriers To Economic Self-Sufficiency, Yolanda Javette Clark Aug 2013

Project-Based Section 8 Housing Participants' Perception Of Cultural And Structural Factors To Explain Barriers To Economic Self-Sufficiency, Yolanda Javette Clark

Dissertations

The Project-Based Section 8 program began as temporary housing of the last resort for people who experience temporary setbacks. However, due to participants' inability to become economically self-sufficient, the anti-poverty program has become permanent housing from which few families have been able to escape. Recent studies related to escaping poverty and becoming economically self-sufficient suggest cultural and structural factors are equally important and collectively impact upward economic mobility.

Similarly, the purpose of this study was to determine if Project-Based Section 8 housing participants' characteristics, described as cultural and structural barriers to economic self-sufficiency, interrelate and collectively predict ability to escape …


Identifying Structural Breaks In The Lebanese Economy 1970-2003: An Application Of The Zivot And Andrews Test, Charles Harvie, Mosayeb Pahlavani, Ali S. Saleh Jul 2013

Identifying Structural Breaks In The Lebanese Economy 1970-2003: An Application Of The Zivot And Andrews Test, Charles Harvie, Mosayeb Pahlavani, Ali S. Saleh

Charles Harvie

During the 1960s and early 1970s the Lebanese economy was characterized by low inflation, high growth, sizeable balance of payments surpluses and small public sector deficits, which made it a highly attractive business centre. During this period the country was described as the Switzerland or Paris of the East. This macroeconomic stability did not last long, however, as the economy subsequently underwent fundamental structural changes during most years after the mid 1970s. The aim of this paper is to identify the timing of major structural breaks in the Lebanese economy by applying the Zivot and Andrews (ZA) (1992) procedure, using …


Structural Breaks, Unit Roots And Postwar Slowdowns In Iranian Economy, Mosayeb Pahlavani, Ed Wilson Jul 2013

Structural Breaks, Unit Roots And Postwar Slowdowns In Iranian Economy, Mosayeb Pahlavani, Ed Wilson

Edgar Wilson

This paper employs annual time series data (1970-2003) using Perron (1997) approaches to determine endogenously the more likely time of major structural breaks in various macroeconomic variables of the Iranian economy.


Advanced Capacity Cost Management Models: Making Visible The Structural Limits Of Capacity, Ted Watts, C J. Mcnair-Connolly, Vicki Baard, Lidija Polutnik Jul 2013

Advanced Capacity Cost Management Models: Making Visible The Structural Limits Of Capacity, Ted Watts, C J. Mcnair-Connolly, Vicki Baard, Lidija Polutnik

Ted Watts

Purpose This paper fills the gap between defining and measuring the productive limits of a machine or system, and the impact of various assumptions about the productive potential of the nature and informativeness of capacity cost management systems. The authors focused on the various ways in which multi-dimensional limits (for example, time, space, volume and/or value-creating ability) can be used to define productive capacity. Specifically, this research suggests that the limits used in establishing the capacity cost management system restricts the amount and nature of the information the system is capable of providing to management.Justification Two reasons are identified for …


Defining The Structural Basis Of Human Plasminogen Binding By Streptococcal Surface Enolase, Amanda J. Cork, Slobodan Jergic, Sven Hammerschmidt, Bostjan Kobe, Vijay Pancholi, Justin L.P. Benesch, Carol V, Robinson, Nicholas E. Dixon, J Andrew Aquilina, Mark J. Walker Jul 2013

Defining The Structural Basis Of Human Plasminogen Binding By Streptococcal Surface Enolase, Amanda J. Cork, Slobodan Jergic, Sven Hammerschmidt, Bostjan Kobe, Vijay Pancholi, Justin L.P. Benesch, Carol V, Robinson, Nicholas E. Dixon, J Andrew Aquilina, Mark J. Walker

Professor Nick E Dixon

The flesh-eating bacterium group A Streptococcus (GAS) binds and activates human plasminogen, promoting invasive disease. Streptococcal surface enolase (SEN), a glycolytic pathway enzyme, is an identified plasminogen receptor of GAS. Here we used mass spectrometry (MS) to confirm that GAS SEN is octameric, thereby validating in silico modeling based on the crystal structure of S. pneumoniae -enolase. Site-directed mutagenesis of surface-located lysine residues (SENK252+255A, SENK304A, SENK334A, SENK344E, SENK435L and SEN434-435) was used to examine their roles in maintaining structural integrity, enzymatic function and plasminogen binding. Structural integrity of the GAS SEN octamer was retained for all mutants except SENK344E, as …


Structural Characterization Of Clusterin-Chaperone Client Protein Complexes, Amy Wyatt, Justin Yerbury, Mark Wilson May 2013

Structural Characterization Of Clusterin-Chaperone Client Protein Complexes, Amy Wyatt, Justin Yerbury, Mark Wilson

Mark R Wilson

Clusterin (CLU) is a potent extracellular chaperone that inhibits protein aggregation and precipitation otherwise caused by physical or chemical stresses (e.g. heat, reduction). This action involves CLU forming soluble high molecular weight (HMW) complexes with the client protein. Other than their unquantified large size, the physical characteristics of these complexes were previously unknown. In this study, HMW CLU-citrate synthase (CS), HMW CLU-fibrinogen (FGN), and HMW CLU-glutathione S-transferase (GST) complexes were generated in vitro, and their structures studied using size exclusion chromatography (SEC), ELISA, SDS-PAGE, dynamic light scattering (DLS), bisANS fluorescence, and circular dichroism spectrophotometry (CD). Densitometry of …