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Full-Text Articles in Social and Behavioral Sciences

Individual Characteristics And Their Effect On Predicting Mu Rhythm Modulation, Adriane Randolph, Melody Jackson, Saurav Karmakar Aug 2014

Individual Characteristics And Their Effect On Predicting Mu Rhythm Modulation, Adriane Randolph, Melody Jackson, Saurav Karmakar

Adriane B. Randolph

Brain-computer interfaces (BCIs) offer users with severe motor disabilities a nonmuscular input channel for communication and control but require that users achieve a level of literacy and be able to harness their appropriate electrophysiological responses for effective use of the interface. There is currently no formalized process for determining a user's aptitude for control of various BCIs without testing on an actual system. This study presents how basic information captured about users may be used to predict modulation of mu rhythms, electrical variations in the motor cortex region of the brain that may be used for control of a BCI. …


Solution Structure Of Domains Iva And V Of The Tau Subunit Of Escherichia Coli Dna Polymerase Iii And Interaction With The Alpha Subunit, Xun-Cheng Su, Slobodan Jergic, Max A Keniry, Nicholas E. Dixon, Gottfried Otting Jul 2013

Solution Structure Of Domains Iva And V Of The Tau Subunit Of Escherichia Coli Dna Polymerase Iii And Interaction With The Alpha Subunit, Xun-Cheng Su, Slobodan Jergic, Max A Keniry, Nicholas E. Dixon, Gottfried Otting

Professor Nick E Dixon

The solution structure of the C-terminal Domain V of the τ subunit of E. coli DNA polymerase III was determined by nuclear magnetic resonance (NMR) spectroscopy. The fold is unique to τ subunits. Amino acid sequence conservation is pronounced for hydrophobic residues that form the structural core of the protein, indicating that the fold is representative for τ subunits from a wide range of different bacteria. The interaction between the polymerase subunits τ and α was studied by NMR experiments where α was incubated with full-length C-terminal domain (τC16), and domains shortened at the C-terminus by 11 and 18 residues, …


Effect Of Protein Stabilization On Charge State Distribution In Positive- And Negative Ion Electrospray Ionization Mass Spectra, Stephen J. Watt, Margaret Sheil, Jennifer L. Beck, Pavel Prosselkov, Gottfried Otting, Nicholas E. Dixon Jul 2013

Effect Of Protein Stabilization On Charge State Distribution In Positive- And Negative Ion Electrospray Ionization Mass Spectra, Stephen J. Watt, Margaret Sheil, Jennifer L. Beck, Pavel Prosselkov, Gottfried Otting, Nicholas E. Dixon

Professor Nick E Dixon

Changes in protein conformation are thought to alter charge state distributions observed in electrospray ionization mass spectra (ESI-MS) of proteins. In most cases, this has been demonstrated by unfolding proteins through acidification of the solution. This methodology changes the properties of the solvent so that changes in the ESI-MS charge envelopes from conformational changes are difficult to separate from the effects of changing solvent on the ionization process. A novel strategy is presented enabling comparison of ESI mass spectra of a folded and partially unfolded protein of the same amino acid sequence subjected to the same experimental protocols and conditions. …


Architecture And Conservation Of The Bacterial Dna Replication Machinery, An Underexploited Drug Target, Andrew Robinson, Rebecca J. Causer, Nicholas E. Dixon Jul 2013

Architecture And Conservation Of The Bacterial Dna Replication Machinery, An Underexploited Drug Target, Andrew Robinson, Rebecca J. Causer, Nicholas E. Dixon

Professor Nick E Dixon

"New antibiotics with novel modes of action are required to combat the growing threat posed by multi-drug resistant bacteria. Over the last decade, genome sequencing and other high-throughput techniques have provided tremendous insight into the molecular processes underlying cellular functions in a wide range of bacterial species. We can now use these data to assess the degree of conservation of certain aspects of bacterial physiology, to help choose the best cellular targets for development of new broad-spectrum antibacterials. DNA replication is a conserved and essential process, and the large number of proteins that interact to replicate DNA in bacteria are …


Defining The Structural Basis Of Human Plasminogen Binding By Streptococcal Surface Enolase, Amanda J. Cork, Slobodan Jergic, Sven Hammerschmidt, Bostjan Kobe, Vijay Pancholi, Justin L.P. Benesch, Carol V, Robinson, Nicholas E. Dixon, J Andrew Aquilina, Mark J. Walker Jul 2013

Defining The Structural Basis Of Human Plasminogen Binding By Streptococcal Surface Enolase, Amanda J. Cork, Slobodan Jergic, Sven Hammerschmidt, Bostjan Kobe, Vijay Pancholi, Justin L.P. Benesch, Carol V, Robinson, Nicholas E. Dixon, J Andrew Aquilina, Mark J. Walker

Professor Nick E Dixon

The flesh-eating bacterium group A Streptococcus (GAS) binds and activates human plasminogen, promoting invasive disease. Streptococcal surface enolase (SEN), a glycolytic pathway enzyme, is an identified plasminogen receptor of GAS. Here we used mass spectrometry (MS) to confirm that GAS SEN is octameric, thereby validating in silico modeling based on the crystal structure of S. pneumoniae -enolase. Site-directed mutagenesis of surface-located lysine residues (SENK252+255A, SENK304A, SENK334A, SENK344E, SENK435L and SEN434-435) was used to examine their roles in maintaining structural integrity, enzymatic function and plasminogen binding. Structural integrity of the GAS SEN octamer was retained for all mutants except SENK344E, as …


Essential Biological Processes Of An Emerging Pathogen: Dna Replication, Transcription, And Cell Division In Acinetobacter Spp., Andrew Robinson, Anthony J. Brzoska, Kylie M. Turner, Ryan Withers, Elizabeth J. Harry, Peter J. Lewis, Nicholas E. Dixon Jul 2013

Essential Biological Processes Of An Emerging Pathogen: Dna Replication, Transcription, And Cell Division In Acinetobacter Spp., Andrew Robinson, Anthony J. Brzoska, Kylie M. Turner, Ryan Withers, Elizabeth J. Harry, Peter J. Lewis, Nicholas E. Dixon

Professor Nick E Dixon

Species of the bacterial genus Acinetobacter are becoming increasingly important as a source of hospital-acquired infections (31, 185, 204). Acinetobacter spp. are ubiquitous nonmotile gammaproteobacteria, typified by metabolic versatility and a capacity for natural transformation (172, 204). The species of most clinical relevance is A. baumannii; however, pathogenic strains of A. lwoffi and A. baylyi have also been described (38, 185, 215).


Incorporation Of Chlorinated Analogues Of Aliphatic Amino Acids During Cell-Free Protein Synthesis, Dannon J. Stigers, Zachary I. Watts, James E. Hennessy, Hye-Kyung Kim, Romeo Martini, Matthew C. Taylor, Kiyoshi Ozawa, Jeffrey W. Keillor, Nicholas E. Dixon, Christopher J. Easton Jul 2013

Incorporation Of Chlorinated Analogues Of Aliphatic Amino Acids During Cell-Free Protein Synthesis, Dannon J. Stigers, Zachary I. Watts, James E. Hennessy, Hye-Kyung Kim, Romeo Martini, Matthew C. Taylor, Kiyoshi Ozawa, Jeffrey W. Keillor, Nicholas E. Dixon, Christopher J. Easton

Professor Nick E Dixon

3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to 90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.


Nanometer-Scale Distance Measurements In Proteins Using Gd3+ Spin Labeling, Alexey Potapov, Hiromasa Yagi, Thomas Huber, Slobodan Jergic, Nicholas E. Dixon, Gottfried Otting, Daniella Goldfarb Jul 2013

Nanometer-Scale Distance Measurements In Proteins Using Gd3+ Spin Labeling, Alexey Potapov, Hiromasa Yagi, Thomas Huber, Slobodan Jergic, Nicholas E. Dixon, Gottfried Otting, Daniella Goldfarb

Professor Nick E Dixon

Methods for measuring nanometer-scale distances between specific sites in proteins are essential for analysis of their structure and function. In this work we introduce Gd3+ spin labeling for nanometer-range distance measurements in proteins by high-field pulse electron paramagnetic resonance (EPR). To evaluate the performance of such measurements, we carried out four-pulse double-electron electron resonance (DEER) measurements on two proteins, p75ICD and τC14, labeled at strategically selected sites with either two nitroxides or two Gd3+ spin labels. In analogy to conventional site-directed spin labeling using nitroxides, Gd3+ tags that are derivatives of dipicolinic acid were covalently attached to cysteine thiol groups. …


Proteomic Dissection Of Dna Polymerization, Jennifer L. Beck, Thitima Urathamakul, Stephen James Watt, Margaret Sheil, Patrick M. Schaeffer, Nicholas E. Dixon Jul 2013

Proteomic Dissection Of Dna Polymerization, Jennifer L. Beck, Thitima Urathamakul, Stephen James Watt, Margaret Sheil, Patrick M. Schaeffer, Nicholas E. Dixon

Professor Nick E Dixon

DNA polymerases replicate the genome by associating with a range of other proteins that enable rapid, high-fidelity copying of DNA. This complex of proteins and nucleic acids is called the replisome. Proteins of the replisome must interact with other networks of proteins, such as those involved in DNA repair. Many of the proteins involved in DNA polymerisation and the accessory proteins are known, but the array of proteins they interact with, and the spatial and temporal arrangement of these interactions is a current research topic. Mass spectrometry is a technique that can be used to identify the sites of these …


Helicase-Binding To Dnai Exposes A Cryptic Dna-Binding Site During Helicase Loading In Bacillus Subtilis, Charikleia Ioannou, Patrick M. Schaeffer, Nicholas E. Dixon, Panos Soultanas Jul 2013

Helicase-Binding To Dnai Exposes A Cryptic Dna-Binding Site During Helicase Loading In Bacillus Subtilis, Charikleia Ioannou, Patrick M. Schaeffer, Nicholas E. Dixon, Panos Soultanas

Professor Nick E Dixon

The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal domain (Nd) that interacts with the replicative ring helicase. A Zn21-binding module mediates the interaction with the helicase and C67, C70 and H84 are involved in the coordination of the Zn21. DnaI binds ATP and exhibits ATPase activity that is not stimulated by ssDNA, because the DNAbinding site on Cd is masked by Nd. The ATPase activity resides on the …


Ultrasensitive Detection Of Antibodies Using A New Tus-Ter-Lock Immunopcr System, Isabelle Morin, Nicholas E. Dixon, Patrick M. Schaeffer Jul 2013

Ultrasensitive Detection Of Antibodies Using A New Tus-Ter-Lock Immunopcr System, Isabelle Morin, Nicholas E. Dixon, Patrick M. Schaeffer

Professor Nick E Dixon

A system consisting of a protein LG coated surface for the capture of mammalian antibodies (target), and an antigen fused to Tus and stoichiometrically linked to a DNA template via the Tus-Ter-lock sequence allowed the ultrasensitive detection of 5.5 attomol of target by real-time immunoPCR in complex media. © The Royal Society of Chemistry 2010.


The Unstructured C-Terminus Of The Tau Subunit Of Escherichia Coli Dna Polymerase Iii Holoenzyme Is The Site Of Interaction With The Alpha Subunit, Slobodan Jergic, Kiyoshi Ozawa, Neal K. Williams, Xun-Cheng Su, Daniel D. Scott, Samir M. Hamdan, Jeffrey A. Crowther, Gottfried Otting, Nicholas E. Dixon Jul 2013

The Unstructured C-Terminus Of The Tau Subunit Of Escherichia Coli Dna Polymerase Iii Holoenzyme Is The Site Of Interaction With The Alpha Subunit, Slobodan Jergic, Kiyoshi Ozawa, Neal K. Williams, Xun-Cheng Su, Daniel D. Scott, Samir M. Hamdan, Jeffrey A. Crowther, Gottfried Otting, Nicholas E. Dixon

Professor Nick E Dixon

The τ subunit of Escherichia coli DNA polymerase III holoenzyme interacts with the α subunit through its C-terminal Domain V, τC16. We show that the extreme C-terminal region of τC16 constitutes the site of interaction with α. The τC16 domain, but not a derivative of it with a C-terminal deletion of seven residues (τC16Δ7), forms an isolable complex with α. Surface plasmon resonance measurements were used to determine the dissociation constant (KD) of the α−τC16 complex to be ∼260 pM. Competition with immobilized τC16 by τC16 derivatives for binding to α gave values of KD of 7 μM for the …


High-Yield Cell-Free Protein Synthesis For Site-Specific Incorporation Of Unnatural Amino Acids At Two Sites, Kiyoshi Ozawa, Karin V. Loscha, Kekini V. Kuppan, Choy Theng Loh, Nicholas E. Dixon, Gottfried Otting Jul 2013

High-Yield Cell-Free Protein Synthesis For Site-Specific Incorporation Of Unnatural Amino Acids At Two Sites, Kiyoshi Ozawa, Karin V. Loscha, Kekini V. Kuppan, Choy Theng Loh, Nicholas E. Dixon, Gottfried Otting

Professor Nick E Dixon

Using aminoacyl-tRNA synthetase/suppressor tRNA pairs derived from Methanocaldococcus jannaschii, an Escherichia coli cell-free protein production system affords proteins with site-specifically incorporated unnatural amino acids (UAAs) in high yields through the use of optimized amber suppressor tRNACUA opt and optimization of reagent concentrations. The efficiency of the cell-free system allows the incorporation of trifluoromethyl-phenylalanine using a polyspecific synthetase evolved previously for p-cyanophenylalanine, and the incorporation of UAAs at two different sites of the same protein without any re-engineering of the E. coli cells used to make the cell-free extract.


Site-Specific Covalent Attachment Of Dna To Proteins Using A Photoactivatable Tus-Ter Complex, Dahdah B. Dahdah, Isabelle Morin, Morgane Moreau, Nicholas E. Dixon, Patrick M. Schaeffer Jul 2013

Site-Specific Covalent Attachment Of Dna To Proteins Using A Photoactivatable Tus-Ter Complex, Dahdah B. Dahdah, Isabelle Morin, Morgane Moreau, Nicholas E. Dixon, Patrick M. Schaeffer

Professor Nick E Dixon

Investigations into the photocrosslinking kinetics of the protein Tus with various bromodeoxyuridine-substituted Ter DNA variants highlight the potential use of this complex as a photoactivatable connector between proteins of interest and specific DNA sequences.


Synthesis And Applications Of Covalent Protein-Dna Conjugates, Patrick M. Schaeffer, Nicholas E. Dixon Jul 2013

Synthesis And Applications Of Covalent Protein-Dna Conjugates, Patrick M. Schaeffer, Nicholas E. Dixon

Professor Nick E Dixon

Synthetic protein-DNA conjugates are valuable tools with applications in fields including nanobiotechnology, bioanalytical chemistry, and molecular diagnostics, and various synthetic methods for their production have been developed during the past three decades. The present article reviews current methodologies for the synthesis of covalent protein-DNA conjugates with particular focus on the regiospecificity and stoichiometry of these reactions.


The Proofreading Exonuclease Subunit E Of Escherichia Coli Dna Polymerase Iii Is Tethered To The Polymerase Subunit A Via A Flexible Linker, Kiyoshi Ozawa, Slobodan Jergic, Ah-Young Park, Nicholas E. Dixon, Gottfried Otting Jul 2013

The Proofreading Exonuclease Subunit E Of Escherichia Coli Dna Polymerase Iii Is Tethered To The Polymerase Subunit A Via A Flexible Linker, Kiyoshi Ozawa, Slobodan Jergic, Ah-Young Park, Nicholas E. Dixon, Gottfried Otting

Professor Nick E Dixon

Escherichia coli DNA polymerase III holoenzyme is composed of 10 different subunits linked by noncovalent interactions. The polymerase activity resides in the α-subunit. The ε-subunit, which contains the proofreading exonuclease site within its N-terminal 185 residues, binds to α via a segment of 57 additional C-terminal residues, and also to θ, whose function is less well defined. The present study shows that θ greatly enhances the solubility of ε during cell-free synthesis. In addition, synthesis of ε in the presence of θ and α resulted in a soluble ternary complex that could readily be purified and analyzed by NMR spectroscopy. …


A Novel Zinc-Binding Fold In The Helicase Interaction Domain Of The Bacillus Subtilis Dnal Helicase Loader, Karin V. Loscha, Kristaps Jaudzems, Charikleia Ioannou, Xun-Cheng Su, Flynn R. Hill, Gottfried Otting, Nicholas E. Dixon, Edvards Liepinsh Jul 2013

A Novel Zinc-Binding Fold In The Helicase Interaction Domain Of The Bacillus Subtilis Dnal Helicase Loader, Karin V. Loscha, Kristaps Jaudzems, Charikleia Ioannou, Xun-Cheng Su, Flynn R. Hill, Gottfried Otting, Nicholas E. Dixon, Edvards Liepinsh

Professor Nick E Dixon

The helicase loader protein DnaI (the Bacillus subtilis homologue of Escherichia coli DnaC) is required to load the hexameric helicase DnaC (the B. subtilis homologue of E. coli DnaB) onto DNA at the start of replication. While the C-terminal domain of DnaI belongs to the structurally well-characterized AAA+ family of ATPases, the structure of the N-terminal domain, DnaI-N, has no homology to a known structure. Three-dimensional structure determination by nuclear magnetic resonance (NMR) spectroscopy shows that DnaI presents a novel fold containing a structurally important zinc ion. Surface plasmon resonance experiments indicate that DnaI-N is largely responsible for binding of …


Inhomogeneities In Yba 2cu 3o 7 Thin Films With Reduced Thickness, O V. Shcherbakova, A V. Pan, S K. Gorman, S A. Fedoseev, I A. Golovchanskiy, S. X. Dou Jun 2013

Inhomogeneities In Yba 2cu 3o 7 Thin Films With Reduced Thickness, O V. Shcherbakova, A V. Pan, S K. Gorman, S A. Fedoseev, I A. Golovchanskiy, S. X. Dou

Shi Xue Dou

Morphology and physical properties of mono- (YBa2Cu3O7) and multilayered (YBa2Cu3O7/SmBa2Cu3O7/ YBa2Cu3O7) superconducting thin films with thickness ranging from 90 nm to 28 nm have been investigated. For both types of samples, the superconducting properties degraded with reduction of film thickness. Structural and electromagnetic properties were visualized through scanning electron microscopy and magneto-optical imaging, respectively, and revealed high level of inhomogeneity for thinner (<58 >nm) samples. However, samples with thickness above 58 nm showed enhanced homogeneity, which …


Progress In Optical Dating Of Guano-Rich Sediments Associated With The Deep Skull, West Mouth Of The Great Cave Of Niah, Sarawak, Borneo, Mark Stephens, Richard Roberts, O Lian, Hiroyuki Yoshida Mar 2013

Progress In Optical Dating Of Guano-Rich Sediments Associated With The Deep Skull, West Mouth Of The Great Cave Of Niah, Sarawak, Borneo, Mark Stephens, Richard Roberts, O Lian, Hiroyuki Yoshida

Richard G Roberts

The West Mouth of the Great Cave of Niah is one of the major archaeological sites in Southeast Asia; the radiocarbon chronology of this site currently places the earliest presence of Homo sapiens (a Deep Skull find) in Southeast Asia at about 45 ka BP. An optical dating programme using sand-sized quartz was initiated in the West Mouth to complement the radiocarbon chronology. This paper reports on the progress in dating a key sample (sample 376r) that was taken from deposits containing the Deep Skull. A somewhat novel procedure was developed to separate and clean quartz grains from the unusual …


Further Evidence For Small-Bodied Hominins From The Late Pleistocene Of Flores Indonesia, Michael Morwood, P. Brown, Jatmiko, T. Sutikna, E.W. Saptomo, Kira Westaway, R. A. Due, Richard Roberts, T Maeda, S Wasisto, T Djubiantono Mar 2013

Further Evidence For Small-Bodied Hominins From The Late Pleistocene Of Flores Indonesia, Michael Morwood, P. Brown, Jatmiko, T. Sutikna, E.W. Saptomo, Kira Westaway, R. A. Due, Richard Roberts, T Maeda, S Wasisto, T Djubiantono

Richard G Roberts

Homo floresiensis was recovered from Late Pleistocene deposits on the island of Flores in eastern Indonesia, but has the stature, limb proportions and endocranial volume of African Pliocene Australopithecus 1. The holotype of the species (LB1), excavated in 2003 from Liang Bua, consisted of a partial skeleton minus the arms. Here we describe additional H. floresiensis remains excavated from the cave in 2004. These include arm bones belonging to the holotype skeleton, a second adult mandible, and postcranial material from other individuals. We can now reconstruct the body proportions of H. floresiensis with some certainty. The finds further demonstrate …


Sediment Mixing At Nonda Rock: Investigations Of Stratigraphic Integrity At An Early Archaeological Site In Northern Australia And Implications For The Human Colonisation Of The Continent, Richard Roberts, C White, L K Fifield, Christian Turney, M Bird, John Tibby, Bruno David, John Magee, Jerome Mialanes Mar 2013

Sediment Mixing At Nonda Rock: Investigations Of Stratigraphic Integrity At An Early Archaeological Site In Northern Australia And Implications For The Human Colonisation Of The Continent, Richard Roberts, C White, L K Fifield, Christian Turney, M Bird, John Tibby, Bruno David, John Magee, Jerome Mialanes

Richard G Roberts

No abstract provided.


Holocene Palaeofire Records In A High-Level, Proximal Valley-Fill (Wilson Bog), Mount Lofty Ranges, South Australia, Solomon Buckman, Katherine Brownlie, Robert Bourman, C. Murray-Wallace, Rowena Morris, Terry Lachlan, Richard Roberts, Lee Arnold, J. Cann Mar 2013

Holocene Palaeofire Records In A High-Level, Proximal Valley-Fill (Wilson Bog), Mount Lofty Ranges, South Australia, Solomon Buckman, Katherine Brownlie, Robert Bourman, C. Murray-Wallace, Rowena Morris, Terry Lachlan, Richard Roberts, Lee Arnold, J. Cann

Richard G Roberts

No abstract provided.


Times Of Sand: Sedimentary History And Archaeology At The Sigatoka Dunes, Fiji, Richard Roberts, Geoffrey Hope, Antoine De Biran, Patrick Nunn, Atholl Anderson, William Dickinson, David Burley, Geoffrey Clark Mar 2013

Times Of Sand: Sedimentary History And Archaeology At The Sigatoka Dunes, Fiji, Richard Roberts, Geoffrey Hope, Antoine De Biran, Patrick Nunn, Atholl Anderson, William Dickinson, David Burley, Geoffrey Clark

Richard G Roberts

No abstract provided.


Equivalent Dose Distributions From Single Grains Of Quartz At Sibudu, South Africa: Context, Causes And Consequences For Optical Dating Of Archaeological Deposits, Richard Roberts, Zenobia Jacobs, Geoffrey Duller, Ann Wintle Mar 2013

Equivalent Dose Distributions From Single Grains Of Quartz At Sibudu, South Africa: Context, Causes And Consequences For Optical Dating Of Archaeological Deposits, Richard Roberts, Zenobia Jacobs, Geoffrey Duller, Ann Wintle

Richard G Roberts

No abstract provided.


Mammalian Responses To Pleistocene Climate Change In Southeastern Australia, Richard Roberts, Jonathon Olley, John Hellstrom, Dirk Megirian, Gavin Prideaux, Kira Westaway Mar 2013

Mammalian Responses To Pleistocene Climate Change In Southeastern Australia, Richard Roberts, Jonathon Olley, John Hellstrom, Dirk Megirian, Gavin Prideaux, Kira Westaway

Richard G Roberts

No abstract provided.


Optically Stimulated Luminescence (Osl) Dating Of Perenially Frozen Deposits In North-Central Siberia: Osl Characteristics Of Quartz Grains And Methodological Considerations Regarding Their Suitability For Dating, Lee Arnold, Richard Roberts Mar 2013

Optically Stimulated Luminescence (Osl) Dating Of Perenially Frozen Deposits In North-Central Siberia: Osl Characteristics Of Quartz Grains And Methodological Considerations Regarding Their Suitability For Dating, Lee Arnold, Richard Roberts

Richard G Roberts

Optically stimulated luminescence (OSL) dating of coarse-grained quartz is increasingly being used as the main chronological tool in late Quaternary palaeoenvironmental reconstructions of Siberia. However, relatively little information has been published on the suitability of OSL dating for the various types of perennially frozen sediments found in this region. Here we provide a systematic examination of the quartz luminescence characteristics of 21 perennially frozen samples from the Taimyr Peninsula and adjacent coastal lowlands of north-central Siberia, and discuss their implications for the reliability of single-grain and multi-grain OSL chronologies in such contexts. The results of this study suggest that the …


Late-Surviving Megafauna In Tasmania Australia, Implicate Human Involvement In Their Extinction, Christian Turney, T Flannery, Richard Roberts, Craig Reid, Keith Fifield, T Higham, Zenobia Jacobs, Noel Kemp, Eric Colhoun, R.M. Kalin Mar 2013

Late-Surviving Megafauna In Tasmania Australia, Implicate Human Involvement In Their Extinction, Christian Turney, T Flannery, Richard Roberts, Craig Reid, Keith Fifield, T Higham, Zenobia Jacobs, Noel Kemp, Eric Colhoun, R.M. Kalin

Richard G Roberts

Establishing the cause of past extinctions is critical if we are to understand better what might trigger future occurrences and how to prevent them. The mechanisms of continental late Pleistocene megafaunal extinction, however, are still fiercely contested. Potential factors contributing to their demise include climatic change, human impact, or some combination. On the Australian mainland, 90% of the megafauna became extinct by ≈46 thousand years (ka) ago, soon after the first archaeological evidence for human colonization of the continent. Yet, on the neighboring island of Tasmania (which was connected to the mainland when sea levels were lower), megafaunal extinction appears …


Evolutionary Anthropology - Homo 'Incendius', Richard Roberts, Michael Bird Mar 2013

Evolutionary Anthropology - Homo 'Incendius', Richard Roberts, Michael Bird

Richard G Roberts

Humans have long been captivated by the flickering flames of the campfire. But when did our ancestors first master the use of fire, and which ancient human species was the first to do so? In Proceedings of the National Academy of Sciences, Berna and colleagues1 report that they have found fragments of burnt bone and ashed plants in one-million-year-old sediments at Wonderwerk Cave, Northern Cape province, South Africa. This evidence of fire occurs in the same sedimentary layers as Acheulian stone tools, usually considered the handiwork of Homo erectus. Their discovery more than doubles the accepted antiquity of the habitual …


The Cyrenaican Prehistory Project 2008: The Second Season Of Investigations Of The Haua Fteah Cave And Its Landscape, And Further Results From The Initial (2007) Fieldwork, Richard Roberts, Zenobia Jacobs, Johnny Morales, M. Morley, R. Grun, L Farr, C Hunt, G Barker, R Rabett, T Reynolds, Victoria Leitch, Robyn Inglis, Roisin Hamilton, John Davison, Hwedi El-Rishi, Alex Pryor, David Simpson, Marijke Van Der Veen, Chris Stimpson, Steven Pawley, Mohammed Touati, Caroline Cartwright, Franca Cole, Iain Morley, Lucilla Burn, Ian Brooks, Laura Basell Mar 2013

The Cyrenaican Prehistory Project 2008: The Second Season Of Investigations Of The Haua Fteah Cave And Its Landscape, And Further Results From The Initial (2007) Fieldwork, Richard Roberts, Zenobia Jacobs, Johnny Morales, M. Morley, R. Grun, L Farr, C Hunt, G Barker, R Rabett, T Reynolds, Victoria Leitch, Robyn Inglis, Roisin Hamilton, John Davison, Hwedi El-Rishi, Alex Pryor, David Simpson, Marijke Van Der Veen, Chris Stimpson, Steven Pawley, Mohammed Touati, Caroline Cartwright, Franca Cole, Iain Morley, Lucilla Burn, Ian Brooks, Laura Basell

Richard G Roberts

No abstract provided.


New Excavations Of Middle Stone Age Deposits At Apollo 11 Rockshelter, Namibia: Stratigraphy, Archaeology, Chronology And Past Environments, Ralf Vogelsang, Jurgen Richter, Zenobia Jacobs, Barbara Eichhorn, Veerle Linseele, Richard Roberts Mar 2013

New Excavations Of Middle Stone Age Deposits At Apollo 11 Rockshelter, Namibia: Stratigraphy, Archaeology, Chronology And Past Environments, Ralf Vogelsang, Jurgen Richter, Zenobia Jacobs, Barbara Eichhorn, Veerle Linseele, Richard Roberts

Richard G Roberts

This paper presents new information obtained from a recent excavation and reassessment of the stratigraphy, chronology, archaeological assemblages and environmental context of the Apollo 11 rockshelter, which contains the longest late Pleistocene and Holocene archaeological sequence in Namibia. The Middle Stone Age (MSA) industries represented at the site include an early MSA, Still Bay, Howieson's Poort and late MSA. Optically stimulated luminescence (OSL) dating of individual quartz grains yielded numerical ages for the Still Bay and Howieson's Poort, and indicated the presence of a post-Howieson's Poort phase. OSL dating also verified conventional and accelerator mass spectrometry radiocarbon ages for a …