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Full-Text Articles in Social and Behavioral Sciences
Evidence Of Animal Mtdna Recombination Between Divergent Populations Of The Potato Cyst Nematode Globodera Pallida, Angelique H. Hoolahan, Vivian C. Blok, Tracey Gibson, Mark Dowton
Evidence Of Animal Mtdna Recombination Between Divergent Populations Of The Potato Cyst Nematode Globodera Pallida, Angelique H. Hoolahan, Vivian C. Blok, Tracey Gibson, Mark Dowton
Faculty of Science - Papers (Archive)
Recombination is typically assumed to be absent in animal mitochondrial genomes (mtDNA). However, the maternal mode of inheritance means that recombinant products are indistinguishable from their progenitor molecules. The majority of studies of mtDNA recombination assess past recombination events, where patterns of recombination are inferred by comparing the mtDNA of different individuals. Few studies assess contemporary mtDNA recombination, where recombinant molecules are observed as direct mosaics of known progenitor molecules. Here we use the potato cyst nematode, Globodera pallida, to investigate past and contemporary recombination. Past recombination was assessed within and between populations of G. pallida, and contemporary …
Poly(T) Variation In Heteroderid Nematode Mitochondrial Genomes Is Predominantly An Artefact Of Amplification, Angelique H. Riepsamen, Tracey A. Gibson, Janet Rowe, David J. Chitwood, Sergei Subbotin, Mark P. Dowton
Poly(T) Variation In Heteroderid Nematode Mitochondrial Genomes Is Predominantly An Artefact Of Amplification, Angelique H. Riepsamen, Tracey A. Gibson, Janet Rowe, David J. Chitwood, Sergei Subbotin, Mark P. Dowton
Faculty of Science - Papers (Archive)
We assessed the rate of in vitro polymerase errors at polythymidine [poly(T)] tracts in the mitochondrial DNA (mtDNA) of a heteroderid nematode (Heterodera cajani). The mtDNA of these nematodes contain unusually high numbers of poly(T) tracts, and have previously been suggested to contain biological poly(T) length variation. However, using a cloned molecule, we observed that poly(T) variation was generated in vitro at regions containing more than six consecutive Ts. This artefactual error rate was estimated at 7.3 × 10−5 indels/poly(T) tract >6 Ts/cycle. This rate was then compared to the rate of poly(T) variation detected after the amplification of a …