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Full-Text Articles in Social and Behavioral Sciences
The Thioflavin T Fluorescence Assay For Amyloid Fibril Detection Can Be Biased By The Presence Of Exogenous Compounds, Sean A. Hudson, Heath Ecroyd, Tak W. Kee, John A. Carver
The Thioflavin T Fluorescence Assay For Amyloid Fibril Detection Can Be Biased By The Presence Of Exogenous Compounds, Sean A. Hudson, Heath Ecroyd, Tak W. Kee, John A. Carver
Faculty of Science - Papers (Archive)
Thioflavin T (ThT) dye fluorescence is used regularly to quantify the formation and inhibition of amyloid fibrils in the presence of anti-amyloidogenic compounds such as polyphenols. However, in this study, it was shown, using three polyphenolics (curcumin, quercetin and resveratrol), that ThT fluorescence should be used with caution in the presence of such exogenous compounds. The strong absorptive and fluorescent properties of quercetin and curcumin were found to significantly bias the ThT fluorescence readings in both in situ real-time ThT assays and single time-point dilution ThT-type assays. The presence of curcumin at concentrations as low as 0.01 and 1 uM …
Visually Directed Walking To Briefly Glimpsed Targets Is Not Biased Toward Fixation Location, John W. Philbeck
Visually Directed Walking To Briefly Glimpsed Targets Is Not Biased Toward Fixation Location, John W. Philbeck
Faculty of Social Sciences - Papers (Archive)
When observers indicate the magnitude of a previously viewed spatial extent by walking without vision to each endpoint, there is little evidence of the perceptual collapse in depth associated with some other methods (eg visual matching). One explanation is that both walking and matching are perceptually mediated, but that the perceived layout is task-dependent. In this view, perceived depth beyond 2 - 3 m is typically distorted by an equidistance effect, whereby the egocentric distances of nonfixated portions of the depth interval are perceptually pulled toward the fixated point. Action-based responses, however, recruit processes that enhance perceptual accuracy as the …