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Full-Text Articles in Social and Behavioral Sciences
Bovine Non-O157 Shiga Toxin 2-Containing Escherichia Coli Isolates Commonly Possess Stx2-Edl933 And/Or Stx2vhb Subtypes, Kim Brett, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven Djordjevic
Bovine Non-O157 Shiga Toxin 2-Containing Escherichia Coli Isolates Commonly Possess Stx2-Edl933 And/Or Stx2vhb Subtypes, Kim Brett, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven Djordjevic
Faculty of Science, Medicine and Health - Papers: part A
stx2 genes from 138 Shiga toxin-producing Escherichia coli (STEC) isolates, of which 127 were of bovine origin (58 serotypes) and 11 of human origin (one serotype; O113:H21), were subtyped. The bovine STEC isolates from Australian cattle carried ehxA and/or eaeA and predominantly possessed stx2-EDL933 (103 of 127; 81.1%) either in combination with stx2vhb (32 of 127; 25.2%) or on its own (52 of 127; 40.4%). Of 22 (90.9%) bovine isolates of serotype O113:H21, a serotype increasingly recovered from patients with hemolytic uremic syndrome (HUS) or hemorrhagic colitis, 20 contained both stx2-EDL933 and stx2vhb; 2 …
Stx1c Is The Most Common Shiga Toxin 1 Subtype Among Shiga Toxin-Producing Escherichia Coli Isolates From Sheep But Not Among Isolates From Cattle, Kim Brett, Vidiya Ramachandran, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven P. Djordjevic
Stx1c Is The Most Common Shiga Toxin 1 Subtype Among Shiga Toxin-Producing Escherichia Coli Isolates From Sheep But Not Among Isolates From Cattle, Kim Brett, Vidiya Ramachandran, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven P. Djordjevic
Faculty of Science, Medicine and Health - Papers: part A
Unlike Shiga toxin 2 (stx2) genes, most nucleotide sequences of Shiga toxin 1 (stx1) genes from Shiga toxin-producing Escherichia coli (STEC), Shigella dysenteriae, and several bacteriophages (H19B, 933J, and H30) are highly conserved. Consequently, there has been little incentive to investigate variants of stx1 among STEC isolates derived from human or animal sources. However stx1OX3, originally identified in an OX3:H8 isolate from a healthy sheep in Germany, differs from other stx1 subtypes by 43 nucleotides, resulting in changes to 12 amino acid residues, and has been renamed stx1c …
Flexibility Revealed By The 1.85 Å Crystal Structure Of The Β Sliding-Clamp Subunit Of Escherichia Coli Dna Polymerase Iii, Aaron J. Oakley, Pavel Prosselkov, Gene Wijffels, Jennifer L. Beck, Matthew Cj Wilce, Nicholas E. Dixon
Flexibility Revealed By The 1.85 Å Crystal Structure Of The Β Sliding-Clamp Subunit Of Escherichia Coli Dna Polymerase Iii, Aaron J. Oakley, Pavel Prosselkov, Gene Wijffels, Jennifer L. Beck, Matthew Cj Wilce, Nicholas E. Dixon
Faculty of Science, Medicine and Health - Papers: part A
The subunit of the Escherichia coli replicative DNA polymerase III holoenzyme is the sliding clamp that interacts with the (polymerase) subunit to maintain the high processivity of the enzyme. The protein is a ring-shaped dimer of 40.6 kDa subunits whose structure has previously been determined at a resolution of 2.5 Å [Kong et al. (1992), Cell, 69, 425-437]. Here, the construction of a new plasmid that directs overproduction of to very high levels and a simple procedure for large-scale purification of the protein are described. Crystals grown under slightly modified conditions diffracted to beyond 1.9 Å at 100 …
Distribution Of Intimin Subtypes Among Escherichia Coli Isolates From Ruminant And Human Sources, Vidiya Ramachandran, Kim Brett, Michael A Hornitzky, Mark Dowton, Karl A Bettelheim, Mark J. Walker, Steven P. Djordjevic
Distribution Of Intimin Subtypes Among Escherichia Coli Isolates From Ruminant And Human Sources, Vidiya Ramachandran, Kim Brett, Michael A Hornitzky, Mark Dowton, Karl A Bettelheim, Mark J. Walker, Steven P. Djordjevic
Faculty of Science, Medicine and Health - Papers: part A
The intimin gene eae, located within the locus of enterocyte effacement pathogenicity island, distinguishes enteropathogenic Escherichia coli (EPEC) and some Shiga toxin-producing E. coli (STEC) strains from all other pathotypes of diarrheagenic E. coli. EPEC is a leading cause of infantile diarrhea in developing countries, and intimin-positive STEC isolates are typically associated with life-threatening diseases such as hemolytic-uremic syndrome and hemorrhagic colitis. Here we describe the development of a PCR-restriction fragment length polymorphism (RFLP) assay that reliably differentiates all 11 known intimin types (α1, α2, β, γ, κ, ɛ, η, ι, λ, θ, and ζ) and three new …