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Articles 271 - 280 of 280
Full-Text Articles in Social and Behavioral Sciences
Considerations For Capping Metal-Contaminated Sediments In Dynamic Estuarine Environments, Stuart L. Simpson, Ian D. Pryor, Ben R. Mewburn, Graeme E. Batley, Dianne F. Jolley
Considerations For Capping Metal-Contaminated Sediments In Dynamic Estuarine Environments, Stuart L. Simpson, Ian D. Pryor, Ben R. Mewburn, Graeme E. Batley, Dianne F. Jolley
Faculty of Science - Papers (Archive)
The effects of tides, bioturbating organisms and periods of anoxia on metal fluxes from contaminated harbour sediments in a shallow tidal estuarine bay were studied, together with capping technology options for the containment of metal contaminants. Zinc fluxes from the sediments were high, ranging from 10 to 89 mg Zn /m2/day. In the absence of capping, experiments in corer reactors showed that simulated tidal processes increased zinc fluxes 5- fold. Fluxes were also greater in the presence of sediment-dwelling organisms. If organisms were removed, and recolonising organisms later added, their bioturbation activities initially lowered zinc fluxes, but fluxes …
Clusterin Is An Extracellular Chaperone That Specifically Interacts With Slowly Aggregating Proteins On Their Off-Folding Pathway, Stephen Poon, T. M. Treweek, Mark R. Wilson, Simon B. Easterbrook-Smith, John A. Carver
Clusterin Is An Extracellular Chaperone That Specifically Interacts With Slowly Aggregating Proteins On Their Off-Folding Pathway, Stephen Poon, T. M. Treweek, Mark R. Wilson, Simon B. Easterbrook-Smith, John A. Carver
Faculty of Science - Papers (Archive)
Clusterin is an extracellular mammalian chaperone protein which inhibits stress-induced precipitation of many different proteins. The conformational state(s) of proteins that interact with clusterin and the stage(s) along the folding and off-folding (precipitation-bound) pathways where this interaction occurs were previously unknown. We investigated this by examining the interactions of clusterin with different structural forms of α-lactalbumin, γ-crystallin and lysozyme. When assessed by ELISA and native gel electrophoresis, clusterin did not bind to various stable, intermediately folded states of α-lactalbumin nor to the native form of this protein, but did bind to and inhibit the slow precipitation of reduced α-lactalbumin. Reduction-induced …
Synthesis Of (+/-)Epipentenomycin I And Iii, Weerachai Phutdhawong, Stephen G. Pyne, Apiwat Baramee, Duang Buddhasukh, Brian W. Skelton, Allan H. White
Synthesis Of (+/-)Epipentenomycin I And Iii, Weerachai Phutdhawong, Stephen G. Pyne, Apiwat Baramee, Duang Buddhasukh, Brian W. Skelton, Allan H. White
Faculty of Science - Papers (Archive)
A synthesis of (±) epipentenomycin I and III is reported from a regioselective epoxidation of racemic 3-hydroxy- and 3-acetoxy-2-methylene-4-cyclopentenone, respectively, with dimethyldioxirane followed by hydrolytic ring-opening of the resulting epoxide.
Mildly Acidic Ph Activates The Extracellular Molecular Chaperone Clusterin, Stephen Poon, M. S. Rybchyn, S. B. Easterbrook-Smith, J. A. Carver, G. J. Pankhurst, Mark R. Wilson
Mildly Acidic Ph Activates The Extracellular Molecular Chaperone Clusterin, Stephen Poon, M. S. Rybchyn, S. B. Easterbrook-Smith, J. A. Carver, G. J. Pankhurst, Mark R. Wilson
Faculty of Science - Papers (Archive)
Many features of the chaperone action of clusterin are similar to those of the intracellular small heat shock proteins (sHSPs) which, like clusterin, exist in solution as heterogeneous aggregates. Increased temperature induces dissociation of some sHSP aggregates and an enhanced chaperone action, suggesting that a dissociated form is the active chaperone species. We recently reported that clusterin aggregates dissociate at mildly acidic pH. To further explore the similarities between clusterin and the sHSPs, we tested the effects of temperature and pH on the structure of clusterin and its chaperone action. Our results demonstrate that increased temperature does not induce dissociation …
Detection Of P2x Purinergic Receptors On Human B Lymphocytes, Ronald Sluyter, J A. Barden, James Wiley
Detection Of P2x Purinergic Receptors On Human B Lymphocytes, Ronald Sluyter, J A. Barden, James Wiley
Faculty of Science - Papers (Archive)
B lymphocytes are known to synthesise the P2X(7) subtype of the P2X purinergic receptor family; however, the identification of the other six P2X subtypes on these cells has been limited by the absence of specific antibodies. In this study, we used a panel of anti-P2X polyclonal antibodies and confocal microscopy to examine the presence of each P2X receptor on human B lymphocytes. We observed that P2X(1), P2X(2), P2X(4) and P2X(7) subtypes, but not P2X(3), P2X(5) and P2X(6) subtypes, are present on B lymphocytes.
Enhanced Tumor Growth In Uv-Irradiated Skin Is Associated With An Influx Of Inflammatory Cells Into The Epidermis, Ronald Sluyter, Gary M. Halliday
Enhanced Tumor Growth In Uv-Irradiated Skin Is Associated With An Influx Of Inflammatory Cells Into The Epidermis, Ronald Sluyter, Gary M. Halliday
Faculty of Science - Papers (Archive)
UV radiation causes a number of cellular changes within the skin which play a role in tumor outgrowth, including immunosuppression and production of growth-enhancing cytokines, Both of these enable tumors to grow but their relative importance in carcinogenesis is poorly defined. In this study, C3H/HeN mice were exposed to a single inflammatory dose of 410 mJ/cm(2) UVB radiation (plus 100 mJ/cm2 UVA radiation) followed by the inoculation of a regressor squamous cell carcinoma into or the painting of oxazolone onto the treated skin. Tumors transplanted 2 or 3 but not 4 days after irradiation had a significantly higher growth rate …
Electrospray Ionisation Mass Spectrometry Of Ruthenium And Palladium Complexes With Oligonucleotides, Jennifer L. Beck, Amy Humphries, Margaret Sheil, Stephen F. Ralph
Electrospray Ionisation Mass Spectrometry Of Ruthenium And Palladium Complexes With Oligonucleotides, Jennifer L. Beck, Amy Humphries, Margaret Sheil, Stephen F. Ralph
Faculty of Science - Papers (Archive)
No abstract provided.
Synthesis Of Thiazole Analogues Of The Immunosuppressive Agent (1r,2s,3r)-2-Acetyl-4(5)-(1,2,3,4-Tetrahydroxybutyl)Imidazole, George R. Jeoffreys, Alison T. Ung, Stephen G. Pyne, Brian W. Skelton, Allan H. White
Synthesis Of Thiazole Analogues Of The Immunosuppressive Agent (1r,2s,3r)-2-Acetyl-4(5)-(1,2,3,4-Tetrahydroxybutyl)Imidazole, George R. Jeoffreys, Alison T. Ung, Stephen G. Pyne, Brian W. Skelton, Allan H. White
Faculty of Science - Papers (Archive)
The synthesis of four of the diastereoisomers of 2-acetyl-5-(1,2,3,4-tetrahydroxybutyl)thiazole and two of the diastereoisomers of 2-acetyl-5-(1,2,3,4,5-pentahydroxypentyl)thiazole and 2-acetyl-4-(1,2,3,4,5-pentahydroxypentyl)thiazole are reported. These syntheses involve the condensation of 5- or 4-metallated 2-(1,1-dimethoxyethyl)thiazoles with 2,3-O-isopropylidene-D-erythrono-1,4-lactone or 5-O-(tert-butyldimethylsilyl)-2,3-O-isopropylidene-D-ribonolactone followed by reductive ring-opening of the resulting lactols. The stereochemistries and structures of some key compounds have been determined by single crystal X-ray structural analysis.
[60]Fullerene Amino Acids And Related Derivatives, Glenn A. Burley, Paul A. Keller, Stephen G. Pyne
[60]Fullerene Amino Acids And Related Derivatives, Glenn A. Burley, Paul A. Keller, Stephen G. Pyne
Faculty of Science - Papers (Archive)
This paper is a review of the literature concerning the preparation of [60]fullerene amino acid and peptide derivatives. The structure and applications of these derivatives to the biological and material sciences is also presented.
Effect Of Organic Solvents On The Separation Of Benzoic Acids By Capillary Electrophoresis, Young J. Lee, William E. Price, Margaret Sheil
Effect Of Organic Solvents On The Separation Of Benzoic Acids By Capillary Electrophoresis, Young J. Lee, William E. Price, Margaret Sheil
Faculty of Science - Papers (Archive)
The effect of organic modifiers on the separation of a number of closely related isomeric benzoic acids by capillary electrophoresis is described. It is shown that while a single modifier concentration cannot help resolve the entire electropherogram, organic modifiers do significantly enhance the resolution of parts of the separation system by comparison with 40 mmol l-1 phosphate buffer. The effects on separation and retention times are discussed in terms of the effects on electroosmotic flow and the electrophoretic mobilities of the charged solutes. The effects were found to be modifier specific, although the trends were in the same direction (ie., …