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Social and Behavioral Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Life Sciences

City University of New York (CUNY)

Student Theses

2018

Sexual Assault Kits

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The Y-Screen Assay: Validation And Evaluation Of Subsequent Str Success, Kassandra Luyando May 2018

The Y-Screen Assay: Validation And Evaluation Of Subsequent Str Success, Kassandra Luyando

Student Theses

There are hundreds of untested sexual assault kits in police custody. As of 2017, New York State passed a law stating that all untested kits must be turned over to forensic laboratories to be tested, causing backlogs at laboratories to increase. The Y-Screen assay is a DNA extraction protocol which utilizes NaOH to rapidly lyse epithelial and sperm cells, and the Quantifiler Trio DNA quantification kit to detect the presence or absence of male DNA prior to standard sample processing. The Y-Screen assay is a commercial product used in this research to test a defined set of validation samples with …