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Social and Behavioral Sciences Commons™
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- Keyword
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- DNA (4)
- Forensic science (4)
- Human trafficking (3)
- MicroRNA (3)
- Raman spectroscopy (3)
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- Body fluids (2)
- Cannabis (2)
- Crime scene (2)
- Exosomes (2)
- Extraction (2)
- Forensic Science (2)
- MicroRNAs (2)
- Semen (2)
- Accipitridae (1)
- Acid phosphatase (1)
- Analytics (1)
- Aquila chrysaetos (1)
- Archaeology (1)
- Australian raven (1)
- Automated particle imaging (1)
- Bald Eagle (1)
- Barium fluoride (1)
- Biomarker (1)
- Biomarkers (1)
- Blood (1)
- Blood enhancement (1)
- Bloodstains (1)
- Blunt force impact patterns (1)
- Body Fluid Identification (1)
- Body Fluids (1)
Articles 1 - 29 of 29
Full-Text Articles in Social and Behavioral Sciences
Investigating The Potential And Pitfalls Of Ev-Encapsulated Micrornas As Circulating Biomarkers Of Breast Cancer, Brian M. Moloney, Katie E. Gilligan, Doireann P. Joyce, Clodagh P. O'Neill, Killian P. O'Brien, Sonja Khan, Claire L. Glynn, Ronan M. Waldron, Ciarán M. Maguire, Emma Holian, Erin Naughton, Mohamed Elhadi, Andrea B. Grealish, Carmel Malone, Emma Mcdermott, Peter Dockery, Thomas Ritter, Adriele Prina-Mello, Michael J. Kerin, Róisín M. Dwyer
Investigating The Potential And Pitfalls Of Ev-Encapsulated Micrornas As Circulating Biomarkers Of Breast Cancer, Brian M. Moloney, Katie E. Gilligan, Doireann P. Joyce, Clodagh P. O'Neill, Killian P. O'Brien, Sonja Khan, Claire L. Glynn, Ronan M. Waldron, Ciarán M. Maguire, Emma Holian, Erin Naughton, Mohamed Elhadi, Andrea B. Grealish, Carmel Malone, Emma Mcdermott, Peter Dockery, Thomas Ritter, Adriele Prina-Mello, Michael J. Kerin, Róisín M. Dwyer
Forensic Science Publications
Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of the releasing cell. We isolated and characterized serum EVs of breast tumour-bearing animals, breast cancer (BC) patients, and healthy controls. EVs were characterized using transmission electron microscopy (TEM), protein quantification, western blotting, and nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed to analyse EV-miR-451a expression. Isolated EVs had the appropriate morphology and size. Patient sera contained significantly more EVs than did healthy controls. In tumour-bearing animals, a correlation between serum EV number and tumour burden was observed. There was no significant relationship …
Potential Applications Of Microrna Profiling To Forensic Investigations, Claire L. Glynn
Potential Applications Of Microrna Profiling To Forensic Investigations, Claire L. Glynn
Forensic Science Publications
Within the forensic science community, there is a continued push to develop novel tools to aid in criminal investigations. microRNA (miRNA) analysis has been the focus of many researcher’s attention in the biomedical field since its discovery in 1993; however, the forensic application of miRNA analysis has only been suggested within the last 10 years and has been gaining considerable traction recently. The primary focus of the forensic application of miRNA analysis has been on body fluid identification to provide confirmatory universal analysis of unknown biological stains obtained from crime scenes or evidence items. There are, however, other forensic applications …
Haplotype Analysis For Irish Ancestry, Heather Miller Coyle, Robert Whiting
Haplotype Analysis For Irish Ancestry, Heather Miller Coyle, Robert Whiting
Forensic Science Publications
Forensic haplotype analysis of the male Y chromosome is currently used to establish the number of male donors in sexual assaults, the number of male bleeders in blood pattern analysis, and for ancestry correlation to genetic founder populations in biogeographic studies. In forensic laboratory applications, its primary use is for DNA profile generation with trace amounts of male DNA in the presence of excess female DNA (e.g. spermatozoa identification, male component of fingernail scrapings). Our study supports the potential use of the Y chromosome in a “dragnet” approach (most haplotypes are unique) similar to that described by Kayser in 2017 …
The Potential Interference Of Body Products And Substrates To The Identification Of Ignitable Liquid Residues On Worn Clothing, Gina Guerrera, Erika Chen, Robert Powers, Brooke Weinger Kammrath
The Potential Interference Of Body Products And Substrates To The Identification Of Ignitable Liquid Residues On Worn Clothing, Gina Guerrera, Erika Chen, Robert Powers, Brooke Weinger Kammrath
Forensic Science Publications
The question of whether deposits on clothing as well as their chemical composition are being mistaken for ignitable fluids is a concern for forensic analysts. Body products and oil secretions can have similar chemical profiles to ignitable liquid residues (ILRs) as a result of comparable chemical compounds that may be found in both sources. This study investigated whether substrates of unworn and worn clothing, with endogenous body secretions and body products could interfere with ILR analysis. Sample extraction was completed by passive headspace concentration with activated charcoal strips (ACS) and desorption with carbon disulfide followed by analysis with gas chromatography-mass …
Optical Tweezers As An Effective Tool For Spermatozoa Isolation From Mixed Forensic Samples, Nicole Auka, Michael Valle, Bobby D. Cox, Peter D. Wilkerson, Tracey Dawson Cruz, Joseph E. Reiner, Sarah J. Seashols-Williams
Optical Tweezers As An Effective Tool For Spermatozoa Isolation From Mixed Forensic Samples, Nicole Auka, Michael Valle, Bobby D. Cox, Peter D. Wilkerson, Tracey Dawson Cruz, Joseph E. Reiner, Sarah J. Seashols-Williams
Forensic Science Publications
A single focus optical tweezer is formed when a laser beam is launched through a high numerical aperture immersion objective. This objective focuses the beam down to a diffraction-limited spot, which creates an optical trap where cells suspended in aqueous solutions can be held fixed. Spermatozoa, an often probative cell type in forensic investigations, can be captured inside this optical trap and dragged one by one across millimeter-length distances in order to create a cluster of cells which can be subsequently drawn up into a capillary for collection. Sperm cells are then ejected onto a sterile cover slip, counted, and …
The Effect Of Electronic Cigarette User Modifications And E-Liquid Adulteration On The Particle Size Profile Of An Aerosolized Product, Haley A. Mulder, Jesse L. Patterson, Matthew S. Halquist, Leon Kosmider, Joseph B. Mcgee Turner, Justin L. Poklis, Alphonse Poklis, Michelle R. Peace
The Effect Of Electronic Cigarette User Modifications And E-Liquid Adulteration On The Particle Size Profile Of An Aerosolized Product, Haley A. Mulder, Jesse L. Patterson, Matthew S. Halquist, Leon Kosmider, Joseph B. Mcgee Turner, Justin L. Poklis, Alphonse Poklis, Michelle R. Peace
Forensic Science Publications
Electronic cigarettes (e-cigarettes) are an alternate nicotine delivery system that generate a condensation aerosol to be inhaled by the user. The size of the droplets formed in the aerosol can vary and contributes to drug deposition and ultimate bioavailability in the lung. The growing popularity of e-cigarette products has caused an increase in internet sources promoting the use of drugs other than nicotine (DOTNs) in e-cigarettes. The purpose of this study was to determine the effect of various e-cigarette and e-liquid modifications, such as coil resistance, battery voltage, and glycol and drug formulation, on the aerosol particle size. E-liquids containing …
Is Forensic Science In Danger Of Extinction?, David San Pietro, Brooke Weinger Kammrath, Peter R. De Forest
Is Forensic Science In Danger Of Extinction?, David San Pietro, Brooke Weinger Kammrath, Peter R. De Forest
Forensic Science Publications
Observations of modern day forensic science has prompted asking the question of whether this field is in danger of extinction. Although there have undoubtedly been meaningful advancements in analytical capabilities, we have overlooked several unintended practical and philosophical consequences. This article addresses three main areas of concern: the declining role of the generalist in an era of increased specialization, the role of education in preparing the next generation of forensic scientists, and the implementation of advanced instrumentation with a focus on statistical significance and field deployable instrumentation.
Investigating The Isolation And Amplification Of Micrornas For Forensic Body Fluid Identification, Kelsie R. O'Leary, Claire L. Glynn
Investigating The Isolation And Amplification Of Micrornas For Forensic Body Fluid Identification, Kelsie R. O'Leary, Claire L. Glynn
Forensic Science Publications
Background: The discovery of forensic DNA typing evolved molecular biology far beyond what could have been expected in terms of its forensic application, and now there exists other developments in molecular biology which are ready for application to forensic challenges. One such challenge is the identification of the body fluid source of stains recovered from evidence items and crime scenes. Currently there are significant efforts in the research field to develop novel methods for the molecular identification of body fluids, with microRNAs (miRNAs) revealing great potential. MiRNAs have been shown to have high tissue specificity and are less susceptible to …
Illicit And Counterfeit Drug Analysis By Morphologically Directed Raman Spectroscopy, Andrew Koutrakos, Pauline E. Leary, Brooke Weinger Kammrath
Illicit And Counterfeit Drug Analysis By Morphologically Directed Raman Spectroscopy, Andrew Koutrakos, Pauline E. Leary, Brooke Weinger Kammrath
Forensic Science Publications
Morphologically directed Raman spectroscopy (MDRS) is a novel tool for the forensic analysis of illicit and counterfeit drug samples. MDRS combines Raman microspectroscopy with automated particle imaging so that physical and chemical information about the components of a mixture sample can be obtained. Results of automated particle imaging are used to determine samples for Raman analysis. The use of MDRS for these types of samples can be employed for both forensic investigations and adjudications of cases. The method provides insight about the physical and chemical composition of the sample, as well as about manufacturing and sample history. Here, MDRS was …
Morphologically Directed Raman Spectroscopic Analysis Of Forensic Samples, Brooke Weinger Kammrath, Andrew Koutrakos, Pauline E. Leary, Josemar A. Castillo, Joe Wolfgang, Deborah Huck-Jones
Morphologically Directed Raman Spectroscopic Analysis Of Forensic Samples, Brooke Weinger Kammrath, Andrew Koutrakos, Pauline E. Leary, Josemar A. Castillo, Joe Wolfgang, Deborah Huck-Jones
Forensic Science Publications
Morphologically directed Raman spectroscopy (MDRS) is a novel and reliable tool that would enable criminalists to obtain more discriminatory information from forensic samples than their current capabilities. MDRS combines automated particle imaging and Raman spectroscopy into one instrument. Particle imaging is performed to determine particle size and shape distributions of components in a blended sample. Particle size is an important physical property of particulate samples and can be used in conjunction with Raman spectroscopy in the analysis of a range of samples of forensic interest, including illicit and counterfeit drugs, soils, gunshot residue (GSR), and white powders. Although measurement of …
Morphologically-Directed Raman Spectroscopy For Forensic Soil Analysis, Brooke Weinger Kammrath, Andrew Koutrakos, Josemar A. Castillo, Cathryn Langley, Debbie Huck-Jones
Morphologically-Directed Raman Spectroscopy For Forensic Soil Analysis, Brooke Weinger Kammrath, Andrew Koutrakos, Josemar A. Castillo, Cathryn Langley, Debbie Huck-Jones
Forensic Science Publications
Morphologically-directed Raman spectroscopy (MDRS) is a novel yet reliable analytical technique that can be used for a variety of forensic applications, enabling scientists to gain more information from samples than they obtain using more traditional methods. In soil forensics, MDRS delivers particle size distribution and microscopic morphological characteristics for the particles present, and at the same time allows secure mineral identification. In this article, we explore the benefits of utilizing soil in forensic investigations, and demonstrate the value of applying MDRS. Two case studies illustrate the real-life potential and applications of this technology.
A Comparative Analysis Of Protein And Peroxidase Blood Enhancement Reagents Following Laundering And Their Impact On Dna Recovery, Gabrielle Hartley, Claire L. Glynn
A Comparative Analysis Of Protein And Peroxidase Blood Enhancement Reagents Following Laundering And Their Impact On Dna Recovery, Gabrielle Hartley, Claire L. Glynn
Forensic Science Publications
Blood is a commonly encountered biological fluid in criminal investigations concerning a violent incident, and visual traces of the fluid on a suspect’s clothing can be diminished through laundering. This study aims to analyze the effects of laundering and the application of commercially available blood enhancement reagents commonly used to improve visualization of dilute bloodstains and their impact on DNA recovery. Enhancement reagents Hungarian Red, Coomassie Blue, Amido Black, luminol, Bluestar® Forensic Magnum, and aqueous Leuco Crystal Violet (LCV) were used to enhance human blood on cotton, polyester, denim, and wool following laundering. DNA was extracted from these samples using …
The Effect Of Fabric Type And Laundering Conditions On The Detection Of Semen Stains, Tyler G. Schlagetter, Claire L. Glynn
The Effect Of Fabric Type And Laundering Conditions On The Detection Of Semen Stains, Tyler G. Schlagetter, Claire L. Glynn
Forensic Science Publications
There has been little research into the effect of fabric type and different laundering conditions on the ability to detect semen stains on washed fabrics. This study aimed to investigate three potential factors affecting semen identification on laundered clothing: fabric type, water temperature during washing, and whether the stain was dry at the time of washing. Following laundering, semen stains on four fabric types (cotton, polyester, denim, and wool) were examined and tested with three common methods used to detect semen; screening with an alternate light source, acid phosphatase press test, and histological staining of spermatazoa. It was determined that …
The Use Of Raman Spectroscopy For The Identification Of Forensically Relevant Body Fluid Stains, Tyler Schlagetter, Claire L. Glynn, Brooke Weinger Kammrath
The Use Of Raman Spectroscopy For The Identification Of Forensically Relevant Body Fluid Stains, Tyler Schlagetter, Claire L. Glynn, Brooke Weinger Kammrath
Forensic Science Publications
Raman spectroscopy is fast becoming a popular technique in the forensic science discipline, and more recently its focus has turned to biological samples. This study reveals the ability of Raman spectroscopy to identify some forensically relevant body fluids, both individually and within mixed samples, that can be crucial in some forensic investigations. This study also further demonstrates the capabilities of Raman as a means for human blood identification in simulated crime scene samples to include bloodstains on a variety of fabrics, at varying dilutions, following laundering, and with the use of various blood-enhancement reagents. The impact of washing and blood-enhancement …
Collecting Quality Infrared Spectra From Microscopic Samples Of Suspicious Powders In A Sealed Cell, Brooke Weinger Kammrath, Pauline E. Leary, John A. Reffner
Collecting Quality Infrared Spectra From Microscopic Samples Of Suspicious Powders In A Sealed Cell, Brooke Weinger Kammrath, Pauline E. Leary, John A. Reffner
Forensic Science Publications
The infrared (IR) microspectroscopical analysis of samples within a sealed-cell containing barium fluoride is a critical need when identifying toxic agents or suspicious powders of unidentified composition. The dispersive nature of barium fluoride is well understood and experimental conditions can be easily adjusted during reflection–absorption measurements to account for differences in focus between the visible and IR regions of the spectrum. In most instances, the ability to collect a viable spectrum is possible when using the sealed cell regardless of whether visible or IR focus is optimized. However, when IR focus is optimized, it is possible to collect useful data …
Species Identification Of Golden And Bald Eagle Talons Using Morphometrics, Avery J. Appleton, R. Christopher O'Brien, Pepper W. Trail
Species Identification Of Golden And Bald Eagle Talons Using Morphometrics, Avery J. Appleton, R. Christopher O'Brien, Pepper W. Trail
Forensic Science Publications
The Golden Eagle (Aquila chrysaetos) and Bald Eagle (Haliaeetus leucocephalus) are the largest avian predators in North America, and are thus species of great ecological importance and cultural significance. There is a long history of human use of eagle body parts, and this use continues today: Bald and Golden eagles are among the North American birds most affected by the illegal wildlife trade. Detached eagle talons are often recovered in both law enforcement and archaeological contexts, but data to allow morphological identification of these talons have been lacking. This study documents measureable differences in the morphology of Bald Eagle and …
Screening Of Exosomal Micrornas From Colorectal Cancer Cells, Cillian Clancy, Sonja Khan, Claire L. Glynn, Emma Holian, Peter Dockery, Pierce Lalor, James A.L. Brown, Myles Joyce, Michael J. Kerin, Roisin M. Dwyer
Screening Of Exosomal Micrornas From Colorectal Cancer Cells, Cillian Clancy, Sonja Khan, Claire L. Glynn, Emma Holian, Peter Dockery, Pierce Lalor, James A.L. Brown, Myles Joyce, Michael J. Kerin, Roisin M. Dwyer
Forensic Science Publications
BACKGROUND: Cells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown. OBJECTIVE: To identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRNAs. METHODS: Exosomes were isolated from HCT-116 and HT-29 cell lines. RNA was extracted from exosomes and microRNA array performed. Cells were engineered to express miR-379 (HCT-116-379) or a non-targeting control (HCT-116-NTC) and functional effects were determined. Exosomes secreted by engineered cells were transferred to recipient cells and the …
Overcoming Human Trafficking Via Operations Research And Analytics: Opportunities For Methods, Models, And Applications, Renata A. Konrad, Andrew C. Trapp, Timothy Palmbach
Overcoming Human Trafficking Via Operations Research And Analytics: Opportunities For Methods, Models, And Applications, Renata A. Konrad, Andrew C. Trapp, Timothy Palmbach
Forensic Science Publications
Human trafficking is a transnational complex societal and economic issue. While human trafficking has been studied in a variety of contexts, including criminology, sociological, and clinical domains, to date there has been very little coverage in the operations research (OR) and analytics community. This paper highlights how operations research and analytics techniques can be used to address the growing issue of human trafficking. It is intended to give insight to operations research and analytics professionals into the unique concerns, problems, and challenges in human trafficking; the relevance of OR and analytics to key pillars of human trafficking including prevention, protection, …
Touch Dna In A Complicated Alleged Child Abuse Case, Heather Miller Coyle
Touch Dna In A Complicated Alleged Child Abuse Case, Heather Miller Coyle
Forensic Science Publications
Touch DNA can be of use in establishing what may have occurred through reconstruction of events based on biological evidence transfer. However, interpretation of results and patterns must be approached with some caution as in the alleged child abuse case detailed here. This case was brought forward as a touch DNA and body fluid case where the male in question was a father reported to have forced a young child to perform oral sex on him. Her pajamas were collected and evaluated for presence of body fluids and associated DNA. The sleeves of the pajamas tested positive for amylase, a …
An Experimental Study To Quantify Error Rates Resulting From Measurement Deviation In Area Of Origin Reconstructions Of Blunt Force Impact Patterns, Mark Davison, Timothy Palmbach
An Experimental Study To Quantify Error Rates Resulting From Measurement Deviation In Area Of Origin Reconstructions Of Blunt Force Impact Patterns, Mark Davison, Timothy Palmbach
Forensic Science Publications
The intent of this study was to attempt to quantify error associated with the measurements required in area of origin reconstructions resulting from the analysis of blunt force impact patterns. Mathematical tables were constructed in order to examine trends associated with changing width and length ratios and the influence of impact angle change and area of convergence deviations. The analysis of the trends enabled informed stain selection, mitigating potential error. The analysis of the influence of stain measurement error and gamma angle error was conducted by reconstructing experimentally created blunt force impact patterns using the Tangent Method, comparing the resulting …
Development Of A Quantitative Real-Time Polymerase Chain Reaction (Rt-Pcr) Assay For Plant Species, Kayla Curtis, Heather Miller Coyle
Development Of A Quantitative Real-Time Polymerase Chain Reaction (Rt-Pcr) Assay For Plant Species, Kayla Curtis, Heather Miller Coyle
Forensic Science Publications
In order to facilitate optimal plant DNA quantitation and identification, an assay has been developed that uses generic plant PCR primers that amplify a region in the chloroplast genome of plant samples. The assay uses the SYBR green detection dye to detect the PCR product with a universal PCR primer set to the large subunit of ribulose bisphosphate carboxylase, rbcL, but can be used with any of the universal barcode primers for land plants (rbcL, matK, trnH, psbA). Standard dilutions of control wheat DNA of varying concentrations were tested to create a standard curve. Several plant DNA extractions of different …
Impact Of Tumour Epithelial Subtype On Circulating Micrornas In Breast Cancer Patients, Peadar S. Waters, Roisin M. Dwyer, Cathy Brougham, Claire L. Glynn, Deidre Wall, Peter Hyland, Maria Duignan, Mark Mcloughlin, John Newell, Michael J. Kerin
Impact Of Tumour Epithelial Subtype On Circulating Micrornas In Breast Cancer Patients, Peadar S. Waters, Roisin M. Dwyer, Cathy Brougham, Claire L. Glynn, Deidre Wall, Peter Hyland, Maria Duignan, Mark Mcloughlin, John Newell, Michael J. Kerin
Forensic Science Publications
While a range of miRNAs have been shown to be dysregulated in the circulation of patients with breast cancer, little is known about the relationship between circulating levels and tumour characteristics. The aim of this study was to analyse alterations in circulating miRNA expression during tumour progression in a murine model of breast cancer, and to detemine the clinical relevance of identified miRNAs at both tissue and circulating level in patient samples. Athymic nude mice received a subcutaneous or mammary fat pad injection of MDA-MB-231 cells. Blood sampling was performed at weeks 1, 3 and 6 following tumour induction, and …
Utilizing Dna Analysis To Combat The World Wide Plague Of Present Day Slavery – Trafficking In Persons, Timothy Palmbach, Jeffrey Bloom, Emily Hoynes, Dragan Primorac, Mario Thomas Gaboury
Utilizing Dna Analysis To Combat The World Wide Plague Of Present Day Slavery – Trafficking In Persons, Timothy Palmbach, Jeffrey Bloom, Emily Hoynes, Dragan Primorac, Mario Thomas Gaboury
Forensic Science Publications
A study was conducted to determine if modern forensic DNA typing methods can be properly employed throughout the world with a final goal of increasing arrests, prosecutions, and convictions of perpetrators of modern day trafficking in persons while concurrently reducing the burden of victim testimony in legal proceedings. Without interruption of investigations, collection of samples containing DNA was conducted in a variety of settings. Evidentiary samples were analyzed on the ANDE Rapid DNA system. Many of the collected swabs yielded informative short tandem repeat profiles with Rapid DNA technology.
Unmasking Cancer As A Consequence Of Human Trafficking: A Multidisciplinary Challenge, Barbara Moynihan, Katherine Olive
Unmasking Cancer As A Consequence Of Human Trafficking: A Multidisciplinary Challenge, Barbara Moynihan, Katherine Olive
Forensic Science Publications
This article will focus on the development of cancer as a potential consequence of human trafficking. Various subtle sequelae of trafficking, such as the insidious development of cancer, may not be seen until well after the victim has been freed. There are a myriad of factors that contribute to missed or inadequate health care for victims and survivors of human trafficking. These health care needs (both medical as well as mental health) may be overlooked until many months or years post-trafficking. We will address the risk factors consistent with human trafficking that should be considered by health care professionals who …
Ground Penetrating Radar Use In Three Contrasting Soil Textures In Southern Ontario, Amanda C. Lowe, David V. Beresford, David O. Carter, Franco Gaspari, R. Christopher O'Brien, Shari L. Forbes
Ground Penetrating Radar Use In Three Contrasting Soil Textures In Southern Ontario, Amanda C. Lowe, David V. Beresford, David O. Carter, Franco Gaspari, R. Christopher O'Brien, Shari L. Forbes
Forensic Science Publications
Ground penetrating radar (GPR) is a non-invasive, geophysical tool that can be used for the identification of clandestine graves. GPR operates by detecting density differences in soil by the transmission of high frequency electromagnetic waves from an antenna. Domestic pig (Sus scrofa domesticus) carcasses were clothed in 100% cotton t-shirts and 50% cotton/50% polyester briefs, and buried at a consistent depth at three field sites of contrasting soil texture (silty clay loam, fine sand and fine sandy loam) in southern Ontario. GPR was used to detect and monitor the graves for a period of 14 months post-burial. Analysis of collected …
Isolation Of Secreted Micrornas (Mirnas) From Cell-Conditioned Media, Claire L. Glynn, Sonja Khan, Michael J. Kerin, Roisin M. Dwyer
Isolation Of Secreted Micrornas (Mirnas) From Cell-Conditioned Media, Claire L. Glynn, Sonja Khan, Michael J. Kerin, Roisin M. Dwyer
Forensic Science Publications
MicroRNAs (miRNAs) have been found to be stable in the circulation of cancer patients raising their potential as circulating biomarkers of disease. The specific source and role, however, of miRNAs in the circulation is unknown and requires elucidation to determine their true potential. In this study, along with primary tissue explants and primary stromal cells, three breast cancer cell lines were employed, including T47D, MDA-MB-231 and SK-BR-3. Tissue explants were harvested in theatre, with informed patient consent, and included tumour, tumour associated normal, and diseased lymph node samples. Cell-conditioned media containing all factors secreted by the cells were harvested. MiRNAs …
Field Testing Of Collection Cards For Cannabis Sativa Samples With A Single Hexanucleotide Dna Marker, Lindsey Allgeier, John Hemenway, Nicholas Shirley, Tommy Lanier, Heather Miller Coyle
Field Testing Of Collection Cards For Cannabis Sativa Samples With A Single Hexanucleotide Dna Marker, Lindsey Allgeier, John Hemenway, Nicholas Shirley, Tommy Lanier, Heather Miller Coyle
Forensic Science Publications
Abstract: The validity and feasibility of using DNA collection cards in the field for preservation and analysis of Cannabis sativa genotypes were investigated using a highly specific hexanucleotide marker. Collection cards were submitted to the National Marijuana Initiative, which selectively trained and managed the collection of specific types of samples from a variety of participating agencies. Samples collected at seizure sites included fresh marijuana leaf samples, dried “dispensary” samples, U.S. border seizures, and hashish. Using a standardized PCR kit with custom-labeled oligonucleotide primers specific to marijuana, collection cards produced eight genotypes and 13 different alleles, extremely low baselines, and no …
The Scavenging Behaviour Of The Australian Raven (Corvus Coronoides): Patterns And Influencing Factors, R. Christopher O'Brien, Alexande Larcombe, Jan Meyer, Shari L. Forbes, Ian Dadour
The Scavenging Behaviour Of The Australian Raven (Corvus Coronoides): Patterns And Influencing Factors, R. Christopher O'Brien, Alexande Larcombe, Jan Meyer, Shari L. Forbes, Ian Dadour
Forensic Science Publications
The Australian Raven (Corvus coronoides) is a widespread, abundant corvid which is often considered a pest species, due to the thought that it predates on livestock, ruin crops, and is often seen feeding on refuse, in both urban and rural areas. The species is known to feed on a range of material from seeds in ploughed fields to human refuse and decomposing organic material. A large proportion of its diet consists of carrion, and as such, the Australian Raven is an effective detrivorous species capable of removing and consuming dead and decomposing carcasses. This research examined the scavenging …
Analysis Of Minerals Using Linearly Polarized Infrared Microspectroscopy, Brooke Weinger Kammrath, Pauline E. Leary, John A. Reffner
Analysis Of Minerals Using Linearly Polarized Infrared Microspectroscopy, Brooke Weinger Kammrath, Pauline E. Leary, John A. Reffner
Forensic Science Publications
This is an extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009.