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Characterization Of The Atcc Α-Tc1-6 Pancreatic Alpha Cell Line To Study Glucagon Secretion, Larissa M. Ikenouye
Characterization Of The Atcc Α-Tc1-6 Pancreatic Alpha Cell Line To Study Glucagon Secretion, Larissa M. Ikenouye
Electronic Theses and Dissertations
The endocrine pancreas is responsible for maintaining glycemic equilibrium in the body. Given the importance of this blood-glucose homeostasis and the implication an unbalance has on Diabetes mellitus, the study of the glucose-sensing alpha and beta cells in the pancreas is a popular field for scientific researchers. In this study, we use immunofluorescence, qPCR analysis, intracellular calcium experiments, and biochemical glucagon secretion assays to determine if the commercially available tumor cell line clone, α-TC1-6 obtained from American Type Culture Collection, is an appropriate model system for glucagon secretion in pancreatic alpha cells. We confirm the production of the hormone peptide …