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Alternating Current Studies And Kinetic Analysis Of Valinomycin Mediated Charge-Transport Through Lipid Bilayer Membranes, Kenneth Lee Cox

Dissertations and Theses

In this study we have investigated the frequency dependence of bilayer lipid membranes for a series of glycerylmonoolein/n-decane bilayers in various aqueous ionic solutions containing the ionophore valinomycin. Reliable values of membrane capacitance and conductance were obtained over the frequency range 0.2 - 200 KHz using an automatic balancing bridge under the control of a microprocessor unit. The admittance data was then normalized and curve-fitted to produce relaxation times and amplitudes from which the kinetic rate parameters, as deduced from a single slab dielectric membrane model, were calculated. These ac experimental rate constants were then compared with those obtained from …