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Full-Text Articles in Physics
The Gene Silencing Protein Morc-1 Topologically Entraps Dna And Forms Multimeric Assemblies To Cause Dna Compaction., Hyeongjun Kim, Linda Yen, Somsakul P. Wongpalee, Jessica A. Kirshner, Nicita Mehta, Yan Xue, Jonathan B. Johnston, Alma L. Burlingame, John K. Kim, Joseph J. Loparo, Steve E. Jacobsen
The Gene Silencing Protein Morc-1 Topologically Entraps Dna And Forms Multimeric Assemblies To Cause Dna Compaction., Hyeongjun Kim, Linda Yen, Somsakul P. Wongpalee, Jessica A. Kirshner, Nicita Mehta, Yan Xue, Jonathan B. Johnston, Alma L. Burlingame, John K. Kim, Joseph J. Loparo, Steve E. Jacobsen
Physics and Astronomy Faculty Publications and Presentations
Highlights
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Caenorhabditis elegans MORC-1 traps DNA loops
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Recruitment of additional MORC-1s cause further loop trapping and DNA compaction
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MORC-1 assemblages become topologically entrapped on DNA
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MORC-1 forms discrete foci in vivo and can phase transition in vitro
Summary
Microrchidia (MORC) ATPases are critical for gene silencing and chromatin compaction in multiple eukaryotic systems, but the mechanisms by which MORC proteins act are poorly understood. Here, we apply a series of biochemical, single-molecule, and cell-based imaging approaches to better understand the function of the Caenorhabditis elegans MORC-1 protein. We find that MORC-1 binds to DNA in a length-dependent but sequence non-specific …