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Advancing Vibrational Spectroscopy For Cellular And Sub Cellular Analysis: Raman Spectroscopy As A Novel In Vitro Nanotoxicological Assessment Protocol, Esen Efeoglu

Doctoral

This work is designed to establish a ‘High content Nanotoxicological Screening method’ using in vitro Raman microspectroscopy. The undeniable increase of nanotechnology based products has brought challenges in terms of determining their toxicological properties. Considering the total time and cost of screening nanomaterials by conventional methods, the need for a rapid, label-free technique which will provide a wide range of information on multiple parameters is unquestionable. This study investigated the applicability of Raman microspectroscopy as a High Content Screening technique to clarify cell-nanoparticle interaction by determining the localisation of the nanoparticles and consequent effects in these localised areas in terms …