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Pressure Induced Structural Changes And Gas Diffusion Pathways In Monomeric Fluorescent Proteins, Yuba R. Bhandari
Pressure Induced Structural Changes And Gas Diffusion Pathways In Monomeric Fluorescent Proteins, Yuba R. Bhandari
FIU Electronic Theses and Dissertations
Fluorescent proteins (FPs) are extremely valuable biochemical markers which have found a wide range of applications in cellular and molecular biology research. The monomeric variants of red fluorescent proteins (RFPs), known as mFruits, have been especially valuable for in vivo applications in mammalian cell imaging. Fluorescent proteins consist of a chromophore caged in the beta-barrel protein scaffold. The photophysical properties of an FP is determined by its chromophore structure and its interactions with the protein barrel.
Application of hydrostatic pressure on FPs results in the modification of the chromophore environment which allows a systematic study of the role of the …