Physics Commons^™

Inhibition Of The Aminopeptidase From Aeromonas Proteolytica By L-Leucinethiol: Kinetic And Spectroscopic Characterization Of A Slow, Tight-Binding Inhibitor–Enzyme Complex, David L. Bienvenue, Brian Bennett, Richard C. Holz

Physics Faculty Research and Publications

The peptide inhibitor l-leucinethiol (LeuSH) was found to be a potent, slow-binding inhibitor of the aminopeptidase from Aeromonas proteolytica (AAP). The overall potency (K_I^*) of LeuSH was 7 nM while the corresponding alcohol l-leucinol (LeuOH) was a simple competitive inhibitor of much lower potency (K_I=17 μM). These data suggest that the free thiol is likely involved in the formation of the E·I and E·I^* complexes, presumably providing a metal ligand. In order to probe the nature of the interaction of LeuSH and LeuOH with the dinuclear active site of AAP, we have …