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Full-Text Articles in Physics
Role Of Beat Noise In Limiting The Sensitivity Of Optical Coherence Tomography, Richard C. Haskell, David Liao, Adam E. Pivonka, Tera L. Bell, Brendan R. Haberle, Barbara M. Hoeling, Daniel C. Petersen
Role Of Beat Noise In Limiting The Sensitivity Of Optical Coherence Tomography, Richard C. Haskell, David Liao, Adam E. Pivonka, Tera L. Bell, Brendan R. Haberle, Barbara M. Hoeling, Daniel C. Petersen
All HMC Faculty Publications and Research
The sensitivity and dynamic range of optical coherence tomography (OCT) are calculated for instruments utilizing two common interferometer configurations and detection schemes. Previous researchers recognized that the performance of dual-balanced OCT instruments is severely limited by beat noise, which is generated by incoherent light backscattered from the sample. However, beat noise has been ignored in previous calculations of Michelson OCT performance. Our measurements of instrument noise confirm the presence of beat noise even in a simple Michelson interferometer configuration with a single photodetector. Including this noise, we calculate the dynamic range as a function of OCT light source power, and …
Transverse Priority Phase Sensitive Optical Coherence Tomography, Jeff Fingler, Jon Williams, Zahid Yaqoob, Changhuei Yang, Richard C. Haskell, Scott E. Fraser
Transverse Priority Phase Sensitive Optical Coherence Tomography, Jeff Fingler, Jon Williams, Zahid Yaqoob, Changhuei Yang, Richard C. Haskell, Scott E. Fraser
All HMC Faculty Publications and Research
A variation on the standard time domain optical coherence tomography (TDOCT) system is presented. Using an inexpensive piezoelectric stack to modulate the reference mirror position, the amplitude and phase of the sample reflection is determined without scanning. With the primary scan in the transverse direction, en face and B-scan OCT images can be readily produced with phase information. This project plans to use the dynamic phase information to add an extra level of contrast to the images, based on the motion of the scatterers.
Visualizing Early Frog Development With Motion-Sensitive 3-D Optical Coherence Microscopy, Richard C. Haskell, Mary E. Williams, Daniel C. Petersen, Barbara M. Hoeling, Andrew J. Schile, J. D. Pennington, M. G. Seetin, J. M. Castelaz, Scott E. Fraser, Cyrus Papan, Hongwu Ren, Johannes F. De Boer, Zhongping Chen
Visualizing Early Frog Development With Motion-Sensitive 3-D Optical Coherence Microscopy, Richard C. Haskell, Mary E. Williams, Daniel C. Petersen, Barbara M. Hoeling, Andrew J. Schile, J. D. Pennington, M. G. Seetin, J. M. Castelaz, Scott E. Fraser, Cyrus Papan, Hongwu Ren, Johannes F. De Boer, Zhongping Chen
All HMC Faculty Publications and Research
A motion-sensitive en-face-scanning 3-D optical coherence microscope (OCM) has been designed and constructed to study critical events in the early development of plants and animals. We describe the OCM instrument and present time-lapse movies of frog gastrulation, an early developmental event in which three distinct tissue layers are established that later give rise to all major organ systems. OCM images constructed with fringe-amplitude data show the mesendoderm migrating up along the blastocoel roof, thus forming the inner two tissue layers. Motion-sigma data, measuring the random motion of scatterers, is used to construct complementary images that indicate the presence of Brownian …
Limits To Performance Improvement Provided By Balanced Interferometers And Balanced Detection In Oct/Ocm Instruments, David Liao, Adam E. Pivonka, Brendan R. Haberle, Daniel C. Petersen, Barbara M. Hoeling, Richard C. Haskell
Limits To Performance Improvement Provided By Balanced Interferometers And Balanced Detection In Oct/Ocm Instruments, David Liao, Adam E. Pivonka, Brendan R. Haberle, Daniel C. Petersen, Barbara M. Hoeling, Richard C. Haskell
All HMC Faculty Publications and Research
We compare the dynamic range of OCT/OCM instruments configured with unbalanced interferometers, e.g., Michelson interferometers, with that of instruments utilizing balanced interferometers and balanced photodetection. We define the dynamic range (DR) as the ratio of the maximum fringe amplitude achieved with a highly reflecting surface to the root-mean-square (rms) noise. Balanced systems achieve a dynamic range 2.5 times higher than that of a Michelson interferometer, enabling an image acquisition speed roughly 6 times faster. This maximum improvement occurs at light source powers of a few milliwatts. At light source powers higher than 30 mW, the advantage in acquisition speed of …
An Optical Coherence Microscope For 3-Dimensional Imaging In Developmental Biology, Barbara M. Hoeling, Andrew D. Fernandez, Richard C. Haskell, Eric Huang, Whittier R. Myers, Daniel C. Petersen, Sharon E. Ungersma, Ruye Wang, Mary E. Williams, Scott E. Fraser
An Optical Coherence Microscope For 3-Dimensional Imaging In Developmental Biology, Barbara M. Hoeling, Andrew D. Fernandez, Richard C. Haskell, Eric Huang, Whittier R. Myers, Daniel C. Petersen, Sharon E. Ungersma, Ruye Wang, Mary E. Williams, Scott E. Fraser
All HMC Faculty Publications and Research
An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 10 mm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.