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Full-Text Articles in Physics
Inhibition Of The Dape-Encoded N-Succinyl- ʟ, ʟ-Diaminopimelic Acid Desuccinylase From Neisseria Meningitidis By ʟ-Captopril, Anna Starus, Boguslaw Nocek, Brian Bennett, James A. Larrabee, Daniel L. Shaw, Wisath Sae-Lee, Marie T. Russo, Danuta M. Gillner, Magdalena Makowska-Grzyska, Andzrej Joachimiak, Richard C. Holz
Inhibition Of The Dape-Encoded N-Succinyl- ʟ, ʟ-Diaminopimelic Acid Desuccinylase From Neisseria Meningitidis By ʟ-Captopril, Anna Starus, Boguslaw Nocek, Brian Bennett, James A. Larrabee, Daniel L. Shaw, Wisath Sae-Lee, Marie T. Russo, Danuta M. Gillner, Magdalena Makowska-Grzyska, Andzrej Joachimiak, Richard C. Holz
Physics Faculty Research and Publications
Binding of the competitive inhibitor ʟ-captopril to the dapE-encoded N-succinyl-ʟ, ʟ-diaminopimelic acid desuccinylase from Neisseria meningitidis (NmDapE) was examined by kinetic, spectroscopic, and crystallographic methods. ʟ-Captopril, an angiotensin-converting enzyme (ACE) inhibitor, was previously shown to be a potent inhibitor of the DapE from Haemophilus influenzae (HiDapE) with an IC50 of 3.3 μM and a measured Ki of 1.8 μM and displayed a dose-responsive antibiotic activity toward Escherichia coli. ʟ-Captopril is also a competitive inhibitor of NmDapE with a Ki of 2.8 μM. To examine the nature of the interaction …
Correlations Between The Electronic Properties Of Shewanella Oneidensis Cytochrome C Nitrite Reductase (Ccnir) And Its Structure: Effects Of Heme Oxidation State And Active Site Ligation, Natalia Stein, Daniel Love, Evan T. Judd, Sean J. Elliot, Brian Bennett, A. Andrew Pacheco
Correlations Between The Electronic Properties Of Shewanella Oneidensis Cytochrome C Nitrite Reductase (Ccnir) And Its Structure: Effects Of Heme Oxidation State And Active Site Ligation, Natalia Stein, Daniel Love, Evan T. Judd, Sean J. Elliot, Brian Bennett, A. Andrew Pacheco
Physics Faculty Research and Publications
The electrochemical properties of Shewanella oneidensis cytochrome c nitrite reductase (ccNiR), a homodimer that contains five hemes per protomer, were investigated by UV–visible and electron paramagnetic resonance (EPR) spectropotentiometries. Global analysis of the UV–vis spectropotentiometric results yielded highly reproducible values for the heme midpoint potentials. These midpoint potential values were then assigned to specific hemes in each protomer (as defined in previous X-ray diffraction studies) by comparing the EPR and UV–vis spectropotentiometric results, taking advantage of the high sensitivity of EPR spectra to the structural microenvironment of paramagnetic centers. Addition of the strong-field ligand cyanide led to a 70 mV …
Conformational Dynamics Of Metallo-Β-Lactamase Ccra During Catalysis Investigated By Using Deer Spectroscopy, Mahesh Aitha, Lindsay Moritz, Indra D. Sahu, Omar Sanyurah, Zahilyn Roche, Robert Mccarrick, Gary A. Lorigan, Brian Bennett, Michael W. Crowder
Conformational Dynamics Of Metallo-Β-Lactamase Ccra During Catalysis Investigated By Using Deer Spectroscopy, Mahesh Aitha, Lindsay Moritz, Indra D. Sahu, Omar Sanyurah, Zahilyn Roche, Robert Mccarrick, Gary A. Lorigan, Brian Bennett, Michael W. Crowder
Physics Faculty Research and Publications
Previous crystallographic and mutagenesis studies have implicated the role of a position-conserved hairpin loop in the metallo-β-lactamases in substrate binding and catalysis. In an effort to probe the motion of that loop during catalysis, rapid-freeze-quench double electron–electron resonance (RFQ-DEER) spectroscopy was used to interrogate metallo-β-lactamase CcrA, which had a spin label at position 49 on the loop and spin labels (at positions 82, 126, or 233) 20–35 Å away from residue 49, during catalysis. At 10 ms after mixing, the DEER spectra show distance increases of 7, 10, and 13 Å between the spin label at position 49 and the …
Epr Methods For Biological Cu(Ii): L-Band Cw And Nars, Brian Bennett, Jason M. Kowalski
Epr Methods For Biological Cu(Ii): L-Band Cw And Nars, Brian Bennett, Jason M. Kowalski
Physics Faculty Research and Publications
Abstract: Copper has many roles in biology that involve the change of coordination sphere and/or oxidation state of the copper ion. Consequently, the study of copper in heterogeneous environments is an important area in biophysics. EPR is a primary technique for the investigation of paramagnetic copper, which is usually the isolated Cu(II) ion, but sometimes as Cu(II) in different oxidation states of multitransition ion clusters. The gross geometry of the coordination environment of Cu(II) can often be determined from a simple inspection of the EPR spectrum, recorded in the traditional X-band frequency range (9–10 GHz). Identification and quantitation of the …