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Full-Text Articles in Physics
Identification Of An Intermediate Species Along The Nitrile Hydratase Reaction Pathway By Epr Spectroscopy, Wasantha Lankathilaka Karunagala Pathiranage, Natalie Gumataotao, Adam T. Fiedler, Richard C. Holz, Brian Bennett
Identification Of An Intermediate Species Along The Nitrile Hydratase Reaction Pathway By Epr Spectroscopy, Wasantha Lankathilaka Karunagala Pathiranage, Natalie Gumataotao, Adam T. Fiedler, Richard C. Holz, Brian Bennett
Physics Faculty Research and Publications
A new method to trap catalytic intermediate species was employed with Fe-type nitrile hydratase from Rhodococcus equi TG328-2 (ReNHase). ReNHase was incubated with substrates in a 23% (w/w) NaCl/H2O eutectic system that remained liquid at −20 °C, thereby permitting the observation of transient species that were present at electron paramagnetic resonance (EPR)-detectable levels in samples frozen while in the steady state. FeIII-EPR signals from the resting enzyme were unaffected by the presence of 23% NaCl, and the catalytic activity was ∼55% that in the absence of NaCl at the optimum pH of 7.5. …
Examination Of The Catalytic Role Of The Axial Cystine Ligand In The Co-Type Nitrile Hydratase From Pseudonocardia Thermophila Jcm 3095, Irene Anyango Ogutu, Martin St. Maurice, Brian Bennett, Richard C. Holz
Examination Of The Catalytic Role Of The Axial Cystine Ligand In The Co-Type Nitrile Hydratase From Pseudonocardia Thermophila Jcm 3095, Irene Anyango Ogutu, Martin St. Maurice, Brian Bennett, Richard C. Holz
Physics Faculty Research and Publications
The strictly conserved αSer162 residue in the Co-type nitrile hydratase from Pseudonocardia thermophila JCM 3095 (PtNHase), which forms a hydrogen bond to the axial αCys108-S atom, was mutated into an Ala residue. The αSer162Ala yielded two different protein species: one was the apoform (αSerA) that exhibited no observable activity, and the second (αSerB) contained its full complement of cobalt ions and was active with a kcat value of 63 ± 3 s−1 towards acrylonitrile at pH 7.5. The X-ray crystal structure of was determined at 1.85 Å resolution and contained no detectable …
Laser Cooling Of Antihydrogen Atoms, C. J. Baker, W. Bertsche, A. Capra, C. Carruth, C. L. Cesar, M. Charlton, A. Christensen, R. Collister, A. Cridland Mathad, S. Eriksson, A. Evans, N. Evetts, J. Fajans, T. Friesen, M. C. Fujiwara, D. R. Gill, P. Grandemange, P. Granum, J. S. Hangst, W. N. Hardy, M. E. Hayden, D. Hodgkinson, E. D. Hunter, C. A. Isaac, M. A. Johnson, J. M. Jones, S. A. Jones, S. Jonsell, A. Khramov, P. Knapp, L. Kurchaninov, N. Madsen, D. Maxwell, J. T. K. Mckenna, S. Menary, J. M. Michan, T. Momose, P. S. Mullan, K. Olchanski, A. Olin, J. Peszka, A. Powell, P. Pusa, C. O. Rasmussen, F. Robicheaux, R. L. Sacramento, M. Sameed, E. Sarid, D. M. Silveira, D, M. Starko, C. So, G. Stutter, Timothy Tharp, A. Thibeault, R. I. Thompson, D. P. Van Der Werf, J. S. Wurtele
Laser Cooling Of Antihydrogen Atoms, C. J. Baker, W. Bertsche, A. Capra, C. Carruth, C. L. Cesar, M. Charlton, A. Christensen, R. Collister, A. Cridland Mathad, S. Eriksson, A. Evans, N. Evetts, J. Fajans, T. Friesen, M. C. Fujiwara, D. R. Gill, P. Grandemange, P. Granum, J. S. Hangst, W. N. Hardy, M. E. Hayden, D. Hodgkinson, E. D. Hunter, C. A. Isaac, M. A. Johnson, J. M. Jones, S. A. Jones, S. Jonsell, A. Khramov, P. Knapp, L. Kurchaninov, N. Madsen, D. Maxwell, J. T. K. Mckenna, S. Menary, J. M. Michan, T. Momose, P. S. Mullan, K. Olchanski, A. Olin, J. Peszka, A. Powell, P. Pusa, C. O. Rasmussen, F. Robicheaux, R. L. Sacramento, M. Sameed, E. Sarid, D. M. Silveira, D, M. Starko, C. So, G. Stutter, Timothy Tharp, A. Thibeault, R. I. Thompson, D. P. Van Der Werf, J. S. Wurtele
Physics Faculty Research and Publications
The photon—the quantum excitation of the electromagnetic field—is massless but carries momentum. A photon can therefore exert a force on an object upon collision1. Slowing the translational motion of atoms and ions by application of such a force2,3, known as laser cooling, was first demonstrated 40 years ago4,5. It revolutionized atomic physics over the following decades6,7,8, and it is now a workhorse in many fields, including studies on quantum degenerate gases, quantum information, atomic clocks and tests of fundamental physics. However, this technique has not yet been applied to antimatter. Here we …