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Determination Of A Molecular Weight Distribution For Intermediate Degradation Products Of Bovine Plasma Albumin, Sherwin M. Beck

Master's Theses

Within the last decade several important studies have been made on the large degradation of products of proteins. However, little work has been done on the intermediate degradation products. This study is concerned with the chromatographic separation of intermediate fragments from chrymotryptie hydrolysates of bovine plasma albumin and the determination of the molecular weights of the fragments. An automatic recording differential refractometer employing a differential cell of original design permitted the observation of chromatographic components as they were eluted f rom a column of Sephadex G-50. A fraction collector of original design collected 3 ml. fractions of the effluent. Three …