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Full-Text Articles in Physics
Nanopore Thermodynamics Via Infrared Laser Heating, Christopher Angevine
Nanopore Thermodynamics Via Infrared Laser Heating, Christopher Angevine
Theses and Dissertations
Single molecule nanopore spectroscopy is a label-free method for characterizing a wide variety of water-soluble molecules. Recently there have been efforts to expand nanopore sensing to new areas of study. Forensic investigators require an easy to deploy method to identify an unknown number of contributors in a solution. Currently there is no easily available method to distinguish between a single or multiple contributor solution of DNA before being processed by more advanced analytical techniques which has led to wasted time and resources increasing the backlog of samples waiting to be processed. In this work we present a new nanopore technique …
Cluster Enhanced Nanopore Spectrometry, Amy Chavis
Cluster Enhanced Nanopore Spectrometry, Amy Chavis
Theses and Dissertations
Nanopore sensing is a label-free method used to characterize water-soluble molecules. Recent work describes how Au25(SG)18 clusters improve the single molecule nanopore spectrometry (SMNS) technique when analyzing polyethylene glycol (PEG). This thesis will further study and optimize the enhancement effect resulting from a cluster’s presence. Additionally, a model describing the interaction between a cluster and PEG is developed to assist in understanding this mechanism of enhancement. This thesis will also discuss expanding the SMNS method to detect peptides, using Au25(SG)18 for enhancement, and adjusting solution conditions to improve the sensitivity of the SMNS system …
Characterization Of Individual Charged Au25(Sg)18 Clusters And Their Enhancement Of Single Molecule Mass Spectrometry, Christopher Angevine
Characterization Of Individual Charged Au25(Sg)18 Clusters And Their Enhancement Of Single Molecule Mass Spectrometry, Christopher Angevine
Theses and Dissertations
Metallic quantum clusters are stable structures that can exhibit many useful magnetic, chemical, and optical properties. Developing clusters for specific applications requires accurate methods for characterizing their physical and chemical properties. Most cluster characterization methods are ensemble-based measurements that can only measure the average values of the cluster properties. Single cluster measurements improve upon this by yielding information about the distribution of cluster parameters. This investigation describes the initial results on a new approach to detecting and characterizing individual gold nanoclusters (Au25(SG)18) in an aqueous solution with nanopore-based resistive pulse sensing. We also present a new application where the clusters …
Analysis Of Polyethylene Glycol In The Α-Hemolysin Nanopore, David M. Dancho
Analysis Of Polyethylene Glycol In The Α-Hemolysin Nanopore, David M. Dancho
Theses and Dissertations
Nanopores have been shown to be a useful analytical tool for single molecule detection. They have been used to study the composition of DNA and other molecules of interest. These pores are usually α-hemolysin which is a toxin from Staphylococcus aureus or more recently nanoscale synthetic solid state pores. Now we are beginning to look at other molecules or proteins by sending them into the nanopores and measuring a characteristic partial current blockade. In this thesis we look at polyethylene glycol (PEG) as it enters and blocks current through a single alpha hemolysin pore. We report the effects of ionic …