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A New Approach To The Toxicity Testing Of Carbon-Based Nanomaterials: The Clonogenic Assay, Eva Herzog, Alan Casey, Fiona Lyng, Gordon Chambers, Hugh Byrne, Maria Davoren
A New Approach To The Toxicity Testing Of Carbon-Based Nanomaterials: The Clonogenic Assay, Eva Herzog, Alan Casey, Fiona Lyng, Gordon Chambers, Hugh Byrne, Maria Davoren
Articles
The cellular toxicity of three types of carbon nanoparticles, namely HiPco® single-walled carbon nanotubes (SWCNT), arc dischargeSWCNTand Printex 90 carbon black nanoparticles,was studied on three different cell models including the human alveolar carcinoma epithelial cell line (A549), the normal human bronchial epithelial cell line (BEAS-2B) and the human keratinocyte cell line (HaCaT) using the clonogenic assay. Carbon nanomaterials are known to interact with colorimetric indicator dyes frequently used in cytotoxicity assays. By employing the clonogenic assay, any such interactions could be avoided, allowing a more reliable method for the in vitro toxicity assessment of carbon-based nanoparticles. It could be shown …